FC930767

Overview
NameFC930767
Unique NameFC930767
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length704
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig11318 contig Ccv1_Contig11318:467..1171. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: ACON_RICTY (Aconitate hydratase OS=Rickettsia typhi GN=acnA PE=3 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 1.535e-47
Identity = 89/159 (55.97%), Postives = 114/159 (71.70%), Query Frame = 3 
Query:  159 IEKLPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTRSENAVKANMEFEFQRNKERFAFLKWGSSAFHNMLVVPPGSGIVHQVNLEYLGRVVF 635
            + KLPYS+R+LLE+ +R   N    KE++    +W  +     EI F PARVL+QDFTGVPA+VDLA MRDAM K+G +  KINPL+PVDLVIDHSV VD    + + + N++ E +RN ER+ FLKWG  AF+N  VVPPG+GI HQVNLEYL +VV+
Sbjct:   36 LNKLPYSLRVLLENVLRTSGN----KENLLVFKEWLKTKKSNTEIDFMPARVLMQDFTGVPAIVDLAAMRDAMQKIGCNPLKINPLIPVDLVIDHSVSVDSYGLKESFEQNVQMEMKRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEYLSKVVW 190

HSP 2 Score: 30.4166 bits (67), Expect = 1.535e-47
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 2 
Query:  644 GMLYPDSVVGTDSHT 688
            G  YPDS+VGTDSHT
Sbjct:  194 GTAYPDSLVGTDSHT 208
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: ACON_STAAR (Aconitate hydratase OS=Staphylococcus aureus (strain MRSA252) GN=acnA PE=3 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 4.426e-47
Identity = 94/177 (53.11%), Postives = 123/177 (69.49%), Query Frame = 3 
Query:  111 GEFGKFYSLPALNDP---RIEKLPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTRSENAVKANMEFEFQRNKERFAFLKWGSSAFHNMLVVPPGSGIVHQVNLEYLGRVV 632
            G+   +Y L A+ +    ++ KLPYSIR+LLES +R  D+F +  + I+ +  +      + E+PFKP+RV+LQDFTGVPAVVDLA +R AM+ +G D  KINP VPVDLVIDHSVQVD   +  A++ NM+ EF+RN ER+ FL W + AF N   VPP +GIVHQVNLEYL  VV
Sbjct:   17 GQSYTYYDLKAVEEQGITKVSKLPYSIRVLLESLLRQEDDFVITDDHIKALSQFGKDG-NEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVV 192

HSP 2 Score: 26.5646 bits (57), Expect = 4.426e-47
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 2 
Query:  653 YPDSVVGTDSHTP*LMG 703
            +PD++VGTDSHT  + G
Sbjct:  204 FPDTLVGTDSHTTMING 220
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: ACON_RICPR (Aconitate hydratase OS=Rickettsia prowazekii GN=acnA PE=3 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 5.770e-47
Identity = 89/167 (53.29%), Postives = 116/167 (69.46%), Query Frame = 3 
Query:  159 IEKLPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTRSENAVKANMEFEFQRNKERFAFLKWGSSAFHNMLVVPPGSGIVHQVNLEYLGRVVFNTTACYTP 659
            ++KLPYS+R+LLE+ +R   N    KE++    +W  +     EI F PARVL+QDFTGVPA+VDLA MRDAM K+G +  KINPL+PVDLVIDHSV VD   ++ +   N+  E +RN ER+ FLKWG  AF+N  VVPPG+GI HQVNLE+L +VV++      P
Sbjct:   36 LKKLPYSLRVLLENVLRTNGN----KENLLVFKEWLKTKKSNTEIDFMPARVLMQDFTGVPAIVDLAAMRDAMQKIGCNPLKINPLIPVDLVIDHSVSVDSYGNKESFDQNVHMEMKRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEFLSQVVWHNDGTAYP 198

HSP 2 Score: 30.4166 bits (67), Expect = 5.770e-47
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 2 
Query:  644 GMLYPDSVVGTDSHT 688
            G  YPDS+VGTDSHT
Sbjct:  194 GTAYPDSLVGTDSHT 208
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: ACON_STAAW (Aconitate hydratase OS=Staphylococcus aureus (strain MW2) GN=acnA PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 1.277e-46
Identity = 95/177 (53.67%), Postives = 122/177 (68.93%), Query Frame = 3 
Query:  111 GEFGKFYSLPALNDPRIEK---LPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTRSENAVKANMEFEFQRNKERFAFLKWGSSAFHNMLVVPPGSGIVHQVNLEYLGRVV 632
            G+   +Y L A+ +  I K   LPYSIR+LLES +R  D+F +  + I+ +  +      + E+PFKP+RV+LQDFTGVPAVVDLA +R AM+ +G D  KINP VPVDLVIDHSVQVD   +  A++ NM+ EF+RN ER+ FL W + AF N   VPP +GIVHQVNLEYL  VV
Sbjct:   17 GQSYTYYDLKAVEEQGITKVSNLPYSIRVLLESLLRQEDDFVITDDHIKALSQFGKDG-NEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVV 192

HSP 2 Score: 26.5646 bits (57), Expect = 1.277e-46
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 2 
Query:  653 YPDSVVGTDSHTP*LMG 703
            +PD++VGTDSHT  + G
Sbjct:  204 FPDTLVGTDSHTTMING 220
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: ACON_STAAS (Aconitate hydratase OS=Staphylococcus aureus (strain MSSA476) GN=acnA PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 1.277e-46
Identity = 95/177 (53.67%), Postives = 122/177 (68.93%), Query Frame = 3 
Query:  111 GEFGKFYSLPALNDPRIEK---LPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTRSENAVKANMEFEFQRNKERFAFLKWGSSAFHNMLVVPPGSGIVHQVNLEYLGRVV 632
            G+   +Y L A+ +  I K   LPYSIR+LLES +R  D+F +  + I+ +  +      + E+PFKP+RV+LQDFTGVPAVVDLA +R AM+ +G D  KINP VPVDLVIDHSVQVD   +  A++ NM+ EF+RN ER+ FL W + AF N   VPP +GIVHQVNLEYL  VV
Sbjct:   17 GQSYTYYDLKAVEEQGITKVSNLPYSIRVLLESLLRQEDDFVITDDHIKALSQFGKDG-NEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVV 192

HSP 2 Score: 26.5646 bits (57), Expect = 1.277e-46
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 2 
Query:  653 YPDSVVGTDSHTP*LMG 703
            +PD++VGTDSHT  + G
Sbjct:  204 FPDTLVGTDSHTTMING 220
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: ACON_STAAN (Aconitate hydratase OS=Staphylococcus aureus (strain N315) GN=acnA PE=1 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 1.277e-46
Identity = 95/177 (53.67%), Postives = 122/177 (68.93%), Query Frame = 3 
Query:  111 GEFGKFYSLPALNDPRIEK---LPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTRSENAVKANMEFEFQRNKERFAFLKWGSSAFHNMLVVPPGSGIVHQVNLEYLGRVV 632
            G+   +Y L A+ +  I K   LPYSIR+LLES +R  D+F +  + I+ +  +      + E+PFKP+RV+LQDFTGVPAVVDLA +R AM+ +G D  KINP VPVDLVIDHSVQVD   +  A++ NM+ EF+RN ER+ FL W + AF N   VPP +GIVHQVNLEYL  VV
Sbjct:   17 GQSYTYYDLKAVEEQGITKVSNLPYSIRVLLESLLRQEDDFVITDDHIKALSQFGKDG-NEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVV 192

HSP 2 Score: 26.5646 bits (57), Expect = 1.277e-46
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 2 
Query:  653 YPDSVVGTDSHTP*LMG 703
            +PD++VGTDSHT  + G
Sbjct:  204 FPDTLVGTDSHTTMING 220
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: ACON_STAAM (Aconitate hydratase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=acnA PE=1 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 1.277e-46
Identity = 95/177 (53.67%), Postives = 122/177 (68.93%), Query Frame = 3 
Query:  111 GEFGKFYSLPALNDPRIEK---LPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTRSENAVKANMEFEFQRNKERFAFLKWGSSAFHNMLVVPPGSGIVHQVNLEYLGRVV 632
            G+   +Y L A+ +  I K   LPYSIR+LLES +R  D+F +  + I+ +  +      + E+PFKP+RV+LQDFTGVPAVVDLA +R AM+ +G D  KINP VPVDLVIDHSVQVD   +  A++ NM+ EF+RN ER+ FL W + AF N   VPP +GIVHQVNLEYL  VV
Sbjct:   17 GQSYTYYDLKAVEEQGITKVSNLPYSIRVLLESLLRQEDDFVITDDHIKALSQFGKDG-NEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVV 192

HSP 2 Score: 26.5646 bits (57), Expect = 1.277e-46
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 2 
Query:  653 YPDSVVGTDSHTP*LMG 703
            +PD++VGTDSHT  + G
Sbjct:  204 FPDTLVGTDSHTTMING 220
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: ACON_STAAC (Aconitate hydratase OS=Staphylococcus aureus (strain COL) GN=acnA PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 1.277e-46
Identity = 95/177 (53.67%), Postives = 122/177 (68.93%), Query Frame = 3 
Query:  111 GEFGKFYSLPALNDPRIEK---LPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTRSENAVKANMEFEFQRNKERFAFLKWGSSAFHNMLVVPPGSGIVHQVNLEYLGRVV 632
            G+   +Y L A+ +  I K   LPYSIR+LLES +R  D+F +  + I+ +  +      + E+PFKP+RV+LQDFTGVPAVVDLA +R AM+ +G D  KINP VPVDLVIDHSVQVD   +  A++ NM+ EF+RN ER+ FL W + AF N   VPP +GIVHQVNLEYL  VV
Sbjct:   17 GQSYTYYDLKAVEEQGITKVSNLPYSIRVLLESLLRQEDDFVITDDHIKALSQFGKDG-NEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVV 192

HSP 2 Score: 26.5646 bits (57), Expect = 1.277e-46
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 2 
Query:  653 YPDSVVGTDSHTP*LMG 703
            +PD++VGTDSHT  + G
Sbjct:  204 FPDTLVGTDSHTTMING 220
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: ACON_MYCAV (Aconitate hydratase OS=Mycobacterium avium GN=acn PE=3 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 3.992e-45
Identity = 89/184 (48.37%), Postives = 120/184 (65.22%), Query Frame = 3 
Query:  108 GGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTRSENAVKANMEFEFQRNKERFAFLKWGSSAFHNMLVVPPGSGIVHQVNLEYLGRVVFNTTACYTP 659
            G +  + Y L A+  P  EKLPYS+++L E+ +RN D   + K+ IE I +W+  A   +EI + PARV++QDFTGVP +VDLA MR+A+  LG +  K+NPL P DLVIDHSV  D+  + +A + N+E E+QRN ER+ FL+W   AF +  VVPP   IVHQVN+EYL RVV        P
Sbjct:   44 GDKSYQIYRLDAV--PNTEKLPYSLKVLAENLLRNEDGSNITKDHIEAIANWDPKAGASIEIQYTPARVVMQDFTGVPCIVDLATMREAIADLGGNPEKVNPLAPADLVIDHSVIADLFGTADAFERNVEIEYQRNGERYQFLRWLQGAFSDFKVVPP-RRIVHQVNIEYLARVVMERVGVAYP 224

HSP 2 Score: 29.261 bits (64), Expect = 3.992e-45
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 2 
Query:  644 GMLYPDSVVGTDSHT 688
            G+ YPD+ VGTDSHT
Sbjct:  220 GVAYPDTCVGTDSHT 234
[Close]
BLAST of FC930767 vs. ExPASy Swiss-Prot
Match: IREB2_MOUSE (Iron-responsive element-binding protein 2 OS=Mus musculus GN=Ireb2 PE=2 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 1.764e-32
Identity = 65/137 (47.45%), Postives = 95/137 (69.34%), Query Frame = 3 
Query:   57 AAEHPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDIEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVTR 467
            +A + F+ ++  L    G    KF+++P L   + + LPYSIR+LLE+A+RNCD F +KKED+  I+DW+      VE+PF PARV+LQDFTG+PA+VD A MR+A+  LG D  K++P  P DL +DHS+Q+D ++
Sbjct:    5 SAGYTFEYLIETL---NGNSQKKFFNVPKLGGTKYDILPYSIRVLLEAAVRNCDGFLMKKEDVMNILDWKTKQ-SNVEVPFFPARVVLQDFTGIPAMVDFAAMREAVKTLGGDPKKVHPACPTDLTVDHSLQIDFSK 137
The following BLAST results are available for this feature:
BLAST of FC930767 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 47
Match NameE-valueIdentityDescription
ACON_RICTY1.535e-4755.97Aconitate hydratase OS=Rickettsia typhi GN=acnA PE... [more]
ACON_STAAR4.426e-4753.11Aconitate hydratase OS=Staphylococcus aureus (stra... [more]
ACON_RICPR5.770e-4753.29Aconitate hydratase OS=Rickettsia prowazekii GN=ac... [more]
ACON_STAAW1.277e-4653.67Aconitate hydratase OS=Staphylococcus aureus (stra... [more]
ACON_STAAS1.277e-4653.67Aconitate hydratase OS=Staphylococcus aureus (stra... [more]
ACON_STAAN1.277e-4653.67Aconitate hydratase OS=Staphylococcus aureus (stra... [more]
ACON_STAAM1.277e-4653.67Aconitate hydratase OS=Staphylococcus aureus (stra... [more]
ACON_STAAC1.277e-4653.67Aconitate hydratase OS=Staphylococcus aureus (stra... [more]
ACON_MYCAV3.992e-4548.37Aconitate hydratase OS=Mycobacterium avium GN=acn ... [more]
IREB2_MOUSE1.764e-3247.45Iron-responsive element-binding protein 2 OS=Mus m... [more]

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Properties
Property NameValue
Genbank descriptionC34108G11EF PostHarvP1 Citrus clementina cDNA clone C34108G11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930767 ID=FC930767; Name=FC930767; organism=Citrus clementina; type=EST; length=704bp
AGATCAGAACCGTTGCTCCTGCCATCGAACGGCTCGAACGCGCCTTTGCT
ACTATGGCTGCTGAACATCCATTCAAGGAGATCTTGACCGCTCTTCCAAA
GCCAGGAGGTGGCGAGTTTGGAAAGTTCTATAGCCTTCCTGCACTCAATG
ATCCAAGAATTGAGAAGCTGCCATACTCTATCAGAATCCTGCTTGAATCT
GCTATTCGTAATTGTGACAACTTTCAAGTTAAGAAGGAAGATATCGAGAA
AATTATTGACTGGGAAAATTCTGCACCAAAGCAAGTTGAAATTCCCTTCA
AGCCTGCTCGTGTATTGTTGCAGGATTTTACTGGAGTACCAGCCGTGGTT
GACCTTGCCTGCATGCGTGATGCTATGAACAAGCTTGGCAGTGATTCCAA
CAAGATCAATCCTCTTGTTCCTGTAGATCTTGTCATTGATCATTCGGTTC
AGGTTGATGTAACAAGATCGGAAAATGCAGTTAAGGCAAATATGGAATTT
GAATTTCAGAGGAACAAGGAGAGATTCGCTTTCCTCAAATGGGGATCTAG
TGCTTTCCATAACATGCTTGTTGTTCCTCCTGGTTCTGGTATTGTGCATC
AGGTCAATCTTGAATATCTGGGACGGGTTGTTTTCAATACCACGGCATGC
TATACCCCGACAGTGTGGTTGGTACTGATTCTCATACACCATGATTGATG
GGTT
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