FC930940

Overview
NameFC930940
Unique NameFC930940
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length518
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA_ASHGO (Glutamine synthetase OS=Ashbya gossypii GN=GLN1 PE=3 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 3.022e-24
Identity = 51/97 (52.58%), Postives = 66/97 (68.04%), Query Frame = 2 
Query:  149 IIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAPAKNFQ 439
            +IAEYIW+  +G  +RSK RTL   V    +LP+WN+DGSSTGQAPG DS+V L P A + DPFRRG+NI+V+ + +   G P   N RH  AK F+
Sbjct:   23 VIAEYIWVDSAG-HLRSKGRTLQRRVESVDELPEWNFDGSSTGQAPGHDSDVYLKPVAFYPDPFRRGDNIIVLAECWNNDGTPNKYNHRHEAAKLFE 118

HSP 2 Score: 26.1794 bits (56), Expect = 3.022e-24
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:  456 AEEPWYGIEQEYTLL-HXD 509
            A + W+G+EQEYTL  H D
Sbjct:  122 AADIWFGLEQEYTLFDHND 140
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA_TUBBO (Glutamine synthetase OS=Tuber borchii GN=GLN1 PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 3.935e-24
Identity = 52/94 (55.32%), Postives = 64/94 (68.09%), Query Frame = 2 
Query:  149 IIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAPAK 430
            I+AEYIWI G+   +RSK +TL    S    LP+WN+DGSSTGQAPGEDS++ L P AIF DPFR G+NILV+ + +   G P   N RH  AK
Sbjct:   26 ILAEYIWIDGAN-GVRSKTKTLFKKPSSVEDLPEWNFDGSSTGQAPGEDSDIYLRPVAIFPDPFRMGDNILVLAECWNADGSPNKFNHRHECAK 118

HSP 2 Score: 28.8758 bits (63), Expect = 3.935e-24
Identity = 9/13 (69.23%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  459 EEPWYGIEQEYTL 497
            ++PW+G+EQEYTL
Sbjct:  126 QKPWFGLEQEYTL 138
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA_COLGL (Glutamine synthetase OS=Colletotrichum gloeosporioides GN=GLN1 PE=3 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.468e-23
Identity = 50/94 (53.19%), Postives = 61/94 (64.89%), Query Frame = 2 
Query:  149 IIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAPAK 430
            I+AEYIWI   G + RSK+RTL      P  LP WN+DGSSTGQAPG++S+V L P A+F DPFR   NILV+ + +   G P   N RH  AK
Sbjct:   26 IMAEYIWIDADG-ETRSKSRTLKEKEYTPEDLPMWNFDGSSTGQAPGDNSDVYLKPVAVFPDPFRGSPNILVLSECWNADGTPNKYNYRHECAK 118

HSP 2 Score: 32.7278 bits (73), Expect = 1.468e-23
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 3 
Query:  456 AEEPWYGIEQEYTLL 500
            A EPW+G+EQEYTLL
Sbjct:  125 AHEPWFGLEQEYTLL 139
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA_DEBHA (Glutamine synthetase OS=Debaryomyces hansenii GN=GLN1 PE=3 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.233e-23
Identity = 48/95 (50.53%), Postives = 63/95 (66.32%), Query Frame = 2 
Query:  146 KIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAPAK 430
            K++AEY+WI   G + RSK +TL    S    LP+WN+DGSSTGQAPG DS+V L P A + DPFR+G+NI+V+ + +   G P   N RH  AK
Sbjct:   22 KVLAEYVWIDADG-NSRSKCKTLDKKPSSVEDLPEWNFDGSSTGQAPGHDSDVYLRPVAFYADPFRKGDNIIVLTECWNNDGTPNKFNHRHESAK 115

HSP 2 Score: 28.1054 bits (61), Expect = 3.233e-23
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  459 EEPWYGIEQEYTL 497
            EE W+G+EQEYTL
Sbjct:  123 EEVWFGLEQEYTL 135
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA_SUIBO (Glutamine synthetase OS=Suillus bovinus GN=GLNA PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 3.236e-23
Identity = 50/96 (52.08%), Postives = 63/96 (65.62%), Query Frame = 2 
Query:  143 DKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAPAK 430
            DK+ AEY+WI G G  +RSK  T+    +D  +L  W++DGSST QAPG DS+V L P AIFKDPFR G+NILV+ + +   G P  TN RH   K
Sbjct:   20 DKVQAEYVWIDGDG-GLRSKTMTMTKKTTDIGQLRIWDFDGSSTNQAPGGDSDVYLRPAAIFKDPFRGGDNILVLAETFNNDGTPNRTNHRHHTKK 114

HSP 2 Score: 29.261 bits (64), Expect = 3.236e-23
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 3 
Query:  465 PWYGIEQEYTLLHXD 509
            PW+G+EQEYTL   D
Sbjct:  124 PWFGLEQEYTLFDAD 138
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA_AMAMU (Glutamine synthetase OS=Amanita muscaria GN=GLN1 PE=2 SV=2)

HSP 1 Score: 98.5969 bits (244), Expect = 3.236e-23
Identity = 51/95 (53.68%), Postives = 62/95 (65.26%), Query Frame = 2 
Query:  146 KIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAPAK 430
            K  AEY+WI G G  +RSK  T+   V+D S+L  W++DGSST QAP  +S+V L P AIFKDPFR G NILV+ + Y   G P  TN RH  AK
Sbjct:   21 KFHAEYVWIDGDG-GLRSKTTTVDQKVTDISQLRVWDFDGSSTNQAPSGNSDVYLRPSAIFKDPFRGGENILVLSETYNNDGTPNRTNHRHHTAK 114

HSP 2 Score: 30.8018 bits (68), Expect = 3.236e-23
Identity = 12/17 (70.59%), Postives = 13/17 (76.47%), Query Frame = 3 
Query:  459 EEPWYGIEQEYTLLHXD 509
            E PW+GIEQEYTL   D
Sbjct:  122 EIPWFGIEQEYTLFDAD 138
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA_EMENI (Glutamine synthetase OS=Emericella nidulans GN=glnA PE=3 SV=2)

HSP 1 Score: 99.3673 bits (246), Expect = 3.238e-23
Identity = 48/94 (51.06%), Postives = 64/94 (68.09%), Query Frame = 2 
Query:  149 IIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAPAK 430
            ++AEYIWI   G   RSK +TL    S   +LP+WN+DGSST QAPG++S+V L P A++ DPFRRG+NILV+C+ +   G P   N RH  A+
Sbjct:   12 VMAEYIWIDAHG-GTRSKTKTLSKAPSSVDELPEWNFDGSSTAQAPGDNSDVYLRPVAMYPDPFRRGDNILVLCETWDSDGSPNKFNYRHDCAR 104

HSP 2 Score: 30.0314 bits (66), Expect = 3.238e-23
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  459 EEPWYGIEQEYTLLHXD 509
            EE W+G+EQEYTLL  D
Sbjct:  112 EEFWFGLEQEYTLLGPD 128
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA_YEAST (Glutamine synthetase OS=Saccharomyces cerevisiae GN=GLN1 PE=1 SV=2)

HSP 1 Score: 106.686 bits (265), Expect = 6.706e-23
Identity = 51/97 (52.58%), Postives = 67/97 (69.07%), Query Frame = 2 
Query:  146 KIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAPAKNF 436
            +IIAEY+WI G+G ++RSK RTL   ++   +LP+WN+DGSST QAPG DS++ L P A + DPFRRG+NI+V+   Y   G P   N RH  AK F
Sbjct:   23 RIIAEYVWIDGTG-NLRSKGRTLKKRITSIDQLPEWNFDGSSTNQAPGHDSDIYLKPVAYYPDPFRRGDNIVVLAACYNNDGTPNKFNHRHEAAKLF 118
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA_KLULA (Glutamine synthetase OS=Kluyveromyces lactis GN=GLN1 PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.144e-22
Identity = 51/97 (52.58%), Postives = 65/97 (67.01%), Query Frame = 2 
Query:  149 IIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAPAKNFQ 439
            IIAEY+WI   G  +RSK RTL   V+    LP+WN+DGSSTGQAPG DS++ L P A + DPFRRG+NI+V+ + +   G P   N RH  AK F+
Sbjct:   26 IIAEYVWIDSEG-GLRSKGRTLNKKVTSVDSLPEWNFDGSSTGQAPGHDSDIYLKPVAFYPDPFRRGDNIVVLAECWNNDGTPNKFNHRHEAAKLFE 121
[Close]
BLAST of FC930940 vs. ExPASy Swiss-Prot
Match: GLNA1_DROME (Glutamine synthetase 1, mitochondrial OS=Drosophila melanogaster GN=Gs1 PE=2 SV=3)

HSP 1 Score: 93.2041 bits (230), Expect = 1.204e-22
Identity = 44/94 (46.81%), Postives = 64/94 (68.09%), Query Frame = 2 
Query:  143 DKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRG-NNILVMCDAYTPAGEPIPTNKRHA 421
            +++ A Y+WI G+G ++R K R L    S    LP W YDGSST QA GE+S+  L P+AI++DPF+ G N+++V+CD Y+  G+P  +NKR A
Sbjct:   60 NRVQATYLWIDGTGENIRLKDRVLDKVPSSVEDLPDWQYDGSSTYQAHGENSDTTLKPRAIYRDPFKPGKNDVIVLCDTYSADGKPTASNKRAA 153

HSP 2 Score: 34.2686 bits (77), Expect = 1.204e-22
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:  459 EEPWYGIEQEYTLLHXD 509
            +EPW+GIEQEYTLL  D
Sbjct:  164 QEPWFGIEQEYTLLDVD 180
The following BLAST results are available for this feature:
BLAST of FC930940 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 84
Match NameE-valueIdentityDescription
GLNA_ASHGO3.022e-2452.58Glutamine synthetase OS=Ashbya gossypii GN=GLN1 PE... [more]
GLNA_TUBBO3.935e-2455.32Glutamine synthetase OS=Tuber borchii GN=GLN1 PE=2... [more]
GLNA_COLGL1.468e-2353.19Glutamine synthetase OS=Colletotrichum gloeosporio... [more]
GLNA_DEBHA3.233e-2350.53Glutamine synthetase OS=Debaryomyces hansenii GN=G... [more]
GLNA_SUIBO3.236e-2352.08Glutamine synthetase OS=Suillus bovinus GN=GLNA PE... [more]
GLNA_AMAMU3.236e-2353.68Glutamine synthetase OS=Amanita muscaria GN=GLN1 P... [more]
GLNA_EMENI3.238e-2351.06Glutamine synthetase OS=Emericella nidulans GN=gln... [more]
GLNA_YEAST6.706e-2352.58Glutamine synthetase OS=Saccharomyces cerevisiae G... [more]
GLNA_KLULA1.144e-2252.58Glutamine synthetase OS=Kluyveromyces lactis GN=GL... [more]
GLNA1_DROME1.204e-2246.81Glutamine synthetase 1, mitochondrial OS=Drosophil... [more]

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Properties
Property NameValue
Genbank descriptionC34110F09EF PostHarvP1 Citrus clementina cDNA clone C34110F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930940 ID=FC930940; Name=FC930940; organism=Citrus clementina; type=EST; length=518bp
GAAGTCTCTGTGTAGCACATTAACATCCCTTCAATCACAGCAATCTCATT
ACCAGTTTCTTTGTTGTGTTAAGAGACTAAAGAGTCTTCGAAACATGTCT
CTGCTCTCAGATCTCCTTAACTTTAACCTCTCAGAATCCACTGACAAGAT
CATCGCTGAGTACATATGGATCGGTGGATCTGGCATGGACATGAGGAGCA
AAGCAAGGACTCTTCCCGGTCCCGTTAGTGATCCTTCCAAGCTGCCTAAG
TGGAATTATGATGGTTCCAGCACAGGCCAAGCACCTGGAGAAGATAGTGA
AGTGATCCTCTATCCTCAAGCTATTTTCAAGGATCCATTCAGGAGAGGCA
ACAATATTCTTGTTATGTGTGATGCTTACACTCCTGCGGGAGAACCAATT
CCAACAAACAAAAGACATGCGCCTGCCAAAAATTTTCAGCCATTCTCAAG
TTGTGGCTGAAGAACCATGGTATGGTATTGAGCAGGAATACACCTTACTG
CACAANGATGTTAAATGG
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