FC930944

Overview
NameFC930944
Unique NameFC930944
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length682
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_STAAS (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain MSSA476) GN=sucD PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.263e-28
Identity = 43/92 (46.74%), Postives = 55/92 (59.78%), Query Frame = 2 
Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            G+PVFN+V EAK ET A  SVIYVP PF            LD+V+CITE IP  DMV+VK  L  + KTRL+GP        ++C++ +  G
Sbjct:   50 GVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVKRYLQGR-KTRLVGPNCPGVITADECKIGIMPG 140

HSP 2 Score: 66.2402 bits (160), Expect = 4.263e-28
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            +VF+DKNT+V+ QGITG    FHT+Q ++YGTK+V GVTP KGG
Sbjct:    2 SVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKGG 45
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_STAAR (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain MRSA252) GN=sucD PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.263e-28
Identity = 43/92 (46.74%), Postives = 55/92 (59.78%), Query Frame = 2 
Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            G+PVFN+V EAK ET A  SVIYVP PF            LD+V+CITE IP  DMV+VK  L  + KTRL+GP        ++C++ +  G
Sbjct:   50 GVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVKRYLQGR-KTRLVGPNCPGVITADECKIGIMPG 140

HSP 2 Score: 66.2402 bits (160), Expect = 4.263e-28
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            +VF+DKNT+V+ QGITG    FHT+Q ++YGTK+V GVTP KGG
Sbjct:    2 SVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKGG 45
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_STAAN (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain N315) GN=sucD PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.263e-28
Identity = 43/92 (46.74%), Postives = 55/92 (59.78%), Query Frame = 2 
Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            G+PVFN+V EAK ET A  SVIYVP PF            LD+V+CITE IP  DMV+VK  L  + KTRL+GP        ++C++ +  G
Sbjct:   50 GVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVKRYLQGR-KTRLVGPNCPGVITADECKIGIMPG 140

HSP 2 Score: 66.2402 bits (160), Expect = 4.263e-28
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            +VF+DKNT+V+ QGITG    FHT+Q ++YGTK+V GVTP KGG
Sbjct:    2 SVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKGG 45
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_STAAM (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=sucD PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.263e-28
Identity = 43/92 (46.74%), Postives = 55/92 (59.78%), Query Frame = 2 
Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            G+PVFN+V EAK ET A  SVIYVP PF            LD+V+CITE IP  DMV+VK  L  + KTRL+GP        ++C++ +  G
Sbjct:   50 GVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVKRYLQGR-KTRLVGPNCPGVITADECKIGIMPG 140

HSP 2 Score: 66.2402 bits (160), Expect = 4.263e-28
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            +VF+DKNT+V+ QGITG    FHT+Q ++YGTK+V GVTP KGG
Sbjct:    2 SVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKGG 45
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_STAAC (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain COL) GN=sucD PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.263e-28
Identity = 43/92 (46.74%), Postives = 55/92 (59.78%), Query Frame = 2 
Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            G+PVFN+V EAK ET A  SVIYVP PF            LD+V+CITE IP  DMV+VK  L  + KTRL+GP        ++C++ +  G
Sbjct:   50 GVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVKRYLQGR-KTRLVGPNCPGVITADECKIGIMPG 140

HSP 2 Score: 66.2402 bits (160), Expect = 4.263e-28
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            +VF+DKNT+V+ QGITG    FHT+Q ++YGTK+V GVTP KGG
Sbjct:    2 SVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKGG 45
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_STAES (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus epidermidis (strain ATCC 12228) GN=sucD PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 7.226e-28
Identity = 42/92 (45.65%), Postives = 56/92 (60.87%), Query Frame = 2 
Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            G+PV+N+V EAK ET AN SV+YVP PF            LD+V+CITE IP  DMV+VK  L  + KTRL+GP        ++C++ +  G
Sbjct:   50 GVPVYNTVEEAKNETGANVSVVYVPAPFAADSIIEAADADLDMVICITEHIPVVDMVKVKRYLQGR-KTRLVGPNCPGVITADECKIGIMPG 140

HSP 2 Score: 64.6994 bits (156), Expect = 7.226e-28
Identity = 27/44 (61.36%), Postives = 36/44 (81.82%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            +VF+DKNT+V+ QGITG    FHT+Q ++YGT++V GVTP KGG
Sbjct:    2 SVFIDKNTKVMVQGITGSTALFHTKQMLDYGTQIVAGVTPGKGG 45
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_STAEQ (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=sucD PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 7.226e-28
Identity = 42/92 (45.65%), Postives = 56/92 (60.87%), Query Frame = 2 
Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            G+PV+N+V EAK ET AN SV+YVP PF            LD+V+CITE IP  DMV+VK  L  + KTRL+GP        ++C++ +  G
Sbjct:   50 GVPVYNTVEEAKNETGANVSVVYVPAPFAADSIIEAADADLDMVICITEHIPVVDMVKVKRYLQGR-KTRLVGPNCPGVITADECKIGIMPG 140

HSP 2 Score: 64.6994 bits (156), Expect = 7.226e-28
Identity = 27/44 (61.36%), Postives = 36/44 (81.82%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            +VF+DKNT+V+ QGITG    FHT+Q ++YGT++V GVTP KGG
Sbjct:    2 SVFIDKNTKVMVQGITGSTALFHTKQMLDYGTQIVAGVTPGKGG 45
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD2_ARCFU (Succinyl-CoA ligase [ADP-forming] subunit alpha 2 OS=Archaeoglobus fulgidus GN=sucD2 PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.810e-27
Identity = 37/81 (45.68%), Postives = 49/81 (60.49%), Query Frame = 2 
Query:  374 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGP 616
            +G TE LG+PV++SV EA  E  ANASVI+VP PF            + ++VCITEGIP HD +++   +     T LIGP
Sbjct:   43 KGGTEVLGVPVYDSVKEAVREADANASVIFVPAPFAADAVMEAADAGIKVIVCITEGIPVHDELKMYWRVKEAGAT-LIGP 122

HSP 2 Score: 62.7734 bits (151), Expect = 3.810e-27
Identity = 27/44 (61.36%), Postives = 34/44 (77.27%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            A+ VD+ T+V+ QGITG  G FHTE+ + YGTK+V GVTP KGG
Sbjct:    2 AIIVDERTKVVVQGITGYQGKFHTERMLNYGTKIVAGVTPGKGG 45

HSP 3 Score: 30.8018 bits (68), Expect = 3.810e-27
Identity = 13/22 (59.09%), Postives = 16/22 (72.73%), Query Frame = 1 
Query:  616 HCPGIIKPGEM*IGIMPGYIHK 681
            +CPGII PG+  +GIMP  I K
Sbjct:  123 NCPGIISPGKTHLGIMPVQIFK 144
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD1_ARCFU (Succinyl-CoA ligase [ADP-forming] subunit alpha 1 OS=Archaeoglobus fulgidus GN=sucD1 PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.128e-25
Identity = 36/81 (44.44%), Postives = 50/81 (61.73%), Query Frame = 2 
Query:  374 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGP 616
            +G +E  G+PV+N+V EA AET A+ S+IYVP  F            L LVVC+TEGIP +D + +   L R+ K+ L+GP
Sbjct:   46 KGGSEVHGIPVYNTVEEAVAETNADTSIIYVPARFATDAIFEAFDAGLRLVVCVTEGIPLYDEMLIHRKL-REVKSMLLGP 125

HSP 2 Score: 63.5438 bits (153), Expect = 1.128e-25
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            A+ VD+NTRVI QGITG  G+F  ++ ++YGTK+VGGVTP KGG
Sbjct:    5 AILVDENTRVIVQGITGFQGSFQAKRMLDYGTKVVGGVTPGKGG 48

HSP 3 Score: 26.9498 bits (58), Expect = 1.128e-25
Identity = 8/17 (47.06%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:  616 HCPGIIKPGEM*IGIMP 666
            +CPG+I PG   +G++P
Sbjct:  126 NCPGVISPGRSKVGLLP 142
[Close]
BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: MTKB_METEA (Malate--CoA ligase subunit alpha OS=Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) GN=mtkB PE=3 SV=2)

HSP 1 Score: 64.3142 bits (155), Expect = 9.063e-25
Identity = 35/84 (41.67%), Postives = 46/84 (54.76%), Query Frame = 2 
Query:  374 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNR---QSKTRLIGP 616
            +G   H G+PVFN+V EA   T A  S+ +V PPF            L LV  IT+GIP  DM+RVK  L R   + +T ++GP
Sbjct:   43 KGGKTHCGVPVFNTVKEAVEATGATTSITFVAPPFAADAIMEAADAGLKLVCSITDGIPAQDMMRVKRYLRRYPKEKRTMVVGP 126

HSP 2 Score: 57.7658 bits (138), Expect = 9.063e-25
Identity = 23/44 (52.27%), Postives = 33/44 (75.00%), Query Frame = 3 
Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            ++ +D+ T ++ QGITG  GTFH ++ I YG+ +VGGVTP KGG
Sbjct:    2 SILIDEKTPILVQGITGDKGTFHAKEMIAYGSNVVGGVTPGKGG 45

HSP 3 Score: 31.5722 bits (70), Expect = 9.063e-25
Identity = 12/21 (57.14%), Postives = 17/21 (80.95%), Query Frame = 1 
Query:  616 HCPGIIKPGEM*IGIMPGYIH 678
            +C GII PG+  +GIMPG+I+
Sbjct:  127 NCAGIISPGKSMLGIMPGHIY 147
The following BLAST results are available for this feature:
BLAST of FC930944 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45
Match NameE-valueIdentityDescription
SUCD_STAAS4.263e-2846.74Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAAR4.263e-2846.74Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAAN4.263e-2846.74Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAAM4.263e-2846.74Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAAC4.263e-2846.74Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAES7.226e-2845.65Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAEQ7.226e-2845.65Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD2_ARCFU3.810e-2745.68Succinyl-CoA ligase [ADP-forming] subunit alpha 2 ... [more]
SUCD1_ARCFU1.128e-2544.44Succinyl-CoA ligase [ADP-forming] subunit alpha 1 ... [more]
MTKB_METEA9.063e-2541.67Malate--CoA ligase subunit alpha OS=Methylobacteri... [more]

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Properties
Property NameValue
Genbank descriptionC34110G01EF PostHarvP1 Citrus clementina cDNA clone C34110G01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930944 ID=FC930944; Name=FC930944; organism=Citrus clementina; type=EST; length=682bp
CGGGGGAGAATCTTTTTCTTCAATATATTTTGGGAAAAAAGAGAGAGACG
AACTCGAGGGAGAAAATCAGCGAATCAGATTAAAGTGAAGTGAATCAAAT
GGCTCGCCAAGCCTTGGCCAAGCTGATCGGCTCGATCGGATGTCGCAGAC
CCTCTACTGCTTCTTCCGTTTCACATTACAGATTATCAACGTCGGCGGCG
CCGGCGGCCCACTCTCGATATTATGGGTCGTCGGCGGCGGCGTCTCAGCC
GGCAGTTTTCGTCGACAAAAACACTCGAGTTATATGCCAAGGCATCACCG
GAAAAAACGGCACGTTCCATACCGAACAAGCCATCGAATACGGCACCAAA
ATGGTGGGTGGCGTGACGCCGAAGAAGGGTGGACTGAGCACTTGGGACTT
CCTGTATTCAACTCGGTTGCTGAAGCTAAGGCTGAAACGAAGGCTAATGC
TTCTGTCATTTATGTTCCTCCGCCATTTGCTGCTGCTGCTATCATGGAGG
CTATGGAGGCTGAGTTGGATCTTGTCGTCTGTATCACCGAAGGAATCCCT
CAACACGATATGGTGCGAGTGAAAGCTGCTCTAAACAGGCAATCTAAAAC
TCGGCTGATTGGTCCCACTGCCCTGGTATCATCAAACCTGGAGAAATGTA
GATTGGCATTATGCCTGGGTATATTCACAAAC
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