FC930974

Overview
NameFC930974
Unique NameFC930974
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length513
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK_OSTTA (S-adenosylmethionine synthetase OS=Ostreococcus tauri GN=METK PE=3 SV=1)

HSP 1 Score: 283.878 bits (725), Expect = 2.968e-76
Identity = 136/170 (80.00%), Postives = 150/170 (88.24%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            TPELMPL+HVLATKLG +LT VRK+GTCPW+ PDGKTQVT+EY NE GAM+P RVHT+LISTQH E V+N++IA DL   VIK V+P+KYLD  TIFHLNPSGRFVIGGP GDAGLTGRKIIIDTYGGW AHGGGAFSGKDPTKVDRSGAYI RQ AKSIVA+GLARR +
Sbjct:  133 TPELMPLTHVLATKLGHRLTVVRKDGTCPWVLPDGKTQVTIEYENEGGAMIPKRVHTILISTQHIEGVSNEKIAEDLMNEVIKKVVPEKYLDADTIFHLNPSGRFVIGGPDGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQVAKSIVAAGLARRAL 302
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK_DICDI (S-adenosylmethionine synthetase OS=Dictyostelium discoideum GN=metK PE=1 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 1.130e-67
Identity = 123/170 (72.35%), Postives = 141/170 (82.94%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            TP LMPL+H LA++L  KLTE+R NGT  W RPD KTQVTVEY  E   + PVRVHT++ISTQHDE VTN++I ADLK+ VIK V+P +YLDD TI+HLNPSGRFVIGGP GD+GLTGRKIIID+YGGW AHGGGAFSGKD TKVDRSGAY  R  AKS+VA+GLA RC+
Sbjct:  133 TPTLMPLTHFLASELVNKLTELRHNGTLAWARPDAKTQVTVEYKKEGHKITPVRVHTIVISTQHDEKVTNEQIRADLKELVIKAVVPAQYLDDNTIYHLNPSGRFVIGGPMGDSGLTGRKIIIDSYGGWGAHGGGAFSGKDATKVDRSGAYAARWIAKSLVAAGLADRCL 302
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK_ACACA (S-adenosylmethionine synthetase OS=Acanthamoeba castellanii GN=metK PE=2 SV=1)

HSP 1 Score: 243.817 bits (621), Expect = 3.403e-64
Identity = 117/170 (68.82%), Postives = 134/170 (78.82%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            TPE MP++HVLAT+L  ++TEVR N    W+RPD KTQVTVEY NENG ++P+R HTV+IS QH E VTN+EI   L + VIKP IP   LD +TIFHLNPSGRFVIGGP G+AGLTG KIIIDTYGGW AHGGGAFSGKDP+KVDRS AY  R  AKS+VA GLA R +
Sbjct:  135 TPEFMPMTHVLATRLCQRMTEVRNNSILSWMRPDAKTQVTVEYRNENGTLIPLRRHTVVISVQHSEDVTNEEIRKRLMEEVIKPSIPAHLLDGETIFHLNPSGRFVIGGPQGEAGLTGGKIIIDTYGGWGAHGGGAFSGKDPSKVDRSAAYAARWIAKSLVARGLANRAL 304
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK2_PONAB (S-adenosylmethionine synthetase isoform type-2 OS=Pongo abelii GN=MAT2A PE=2 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 4.445e-64
Identity = 117/170 (68.82%), Postives = 135/170 (79.41%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            T E MPL+ VLA KL AKL E+R+NGT PWLRPD KTQVTV+Y  + GA++P+RVHT++IS QHDE V  DE+   LK+ VIK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGW AHGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +
Sbjct:  146 TEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVL 315
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK2_MOUSE (S-adenosylmethionine synthetase isoform type-2 OS=Mus musculus GN=Mat2a PE=2 SV=2)

HSP 1 Score: 243.432 bits (620), Expect = 4.445e-64
Identity = 117/170 (68.82%), Postives = 135/170 (79.41%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            T E MPL+ VLA KL AKL E+R+NGT PWLRPD KTQVTV+Y  + GA++P+RVHT++IS QHDE V  DE+   LK+ VIK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGW AHGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +
Sbjct:  146 TEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVL 315
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK2_MACFA (S-adenosylmethionine synthetase isoform type-2 OS=Macaca fascicularis GN=MAT2A PE=2 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 4.445e-64
Identity = 117/170 (68.82%), Postives = 135/170 (79.41%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            T E MPL+ VLA KL AKL E+R+NGT PWLRPD KTQVTV+Y  + GA++P+RVHT++IS QHDE V  DE+   LK+ VIK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGW AHGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +
Sbjct:  146 TEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVL 315
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK2_HUMAN (S-adenosylmethionine synthetase isoform type-2 OS=Homo sapiens GN=MAT2A PE=1 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 4.445e-64
Identity = 117/170 (68.82%), Postives = 135/170 (79.41%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            T E MPL+ VLA KL AKL E+R+NGT PWLRPD KTQVTV+Y  + GA++P+RVHT++IS QHDE V  DE+   LK+ VIK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGW AHGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +
Sbjct:  146 TEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVL 315
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK2_RAT (S-adenosylmethionine synthetase isoform type-2 OS=Rattus norvegicus GN=Mat2a PE=2 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 5.805e-64
Identity = 116/170 (68.24%), Postives = 135/170 (79.41%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            T E MPL+ VLA KL AKL E+R+NGT PWLRPD KTQVTV+Y  + GA++P+RVHT++IS QHDE V  DE+   LK+ +IK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGW AHGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +
Sbjct:  146 TEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVIPIRVHTIVISVQHDEEVCLDEMRDALKEKLIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVL 315
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK1_HUMAN (S-adenosylmethionine synthetase isoform type-1 OS=Homo sapiens GN=MAT1A PE=1 SV=2)

HSP 1 Score: 235.728 bits (600), Expect = 9.268e-62
Identity = 107/170 (62.94%), Postives = 137/170 (80.59%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            T E MPL+ +LA KL A++ ++R++G  PWLRPD KTQVTV+Y  +NGA++PVR+HT++IS QH+E +T +E+   LK+ VI+ V+P KYLD+ T++HL PSGRFVIGGP GDAG+TGRKII+DTYGGW AHGGGAFSGKD TKVDRS AY  R  AKS+V +GL RR +
Sbjct:  146 TEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVL 315
[Close]
BLAST of FC930974 vs. ExPASy Swiss-Prot
Match: METK1_RAT (S-adenosylmethionine synthetase isoform type-1 OS=Rattus norvegicus GN=Mat1a PE=1 SV=2)

HSP 1 Score: 234.572 bits (597), Expect = 2.065e-61
Identity = 109/170 (64.12%), Postives = 135/170 (79.41%), Query Frame = 3 
Query:    3 TPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWXAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCI 512
            T E MPL+ VLA KL  ++ ++R++G  PWLRPD KTQVTV+Y  +NGA++PVRVHT++IS QH+E +T + +   LK+ VIK V+P KYLD+ TI+HL PSGRFVIGGP GDAG+TGRKII+DTYGGW AHGGGAFSGKD TKVDRS AY  R  AKS+V +GL RR +
Sbjct:  147 TEECMPLTIVLAHKLNTRMADLRRSGVLPWLRPDSKTQVTVQYVQDNGAVIPVRVHTIVISVQHNEDITLEAMREALKEQVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVL 316
The following BLAST results are available for this feature:
BLAST of FC930974 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_OSTTA2.968e-7680.00S-adenosylmethionine synthetase OS=Ostreococcus ta... [more]
METK_DICDI1.130e-6772.35S-adenosylmethionine synthetase OS=Dictyostelium d... [more]
METK_ACACA3.403e-6468.82S-adenosylmethionine synthetase OS=Acanthamoeba ca... [more]
METK2_PONAB4.445e-6468.82S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_MOUSE4.445e-6468.82S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_MACFA4.445e-6468.82S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_HUMAN4.445e-6468.82S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_RAT5.805e-6468.24S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK1_HUMAN9.268e-6262.94S-adenosylmethionine synthetase isoform type-1 OS=... [more]
METK1_RAT2.065e-6164.12S-adenosylmethionine synthetase isoform type-1 OS=... [more]

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Properties
Property NameValue
Genbank descriptionC34100A09EF PostHarvP1 Citrus clementina cDNA clone C34100A09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930974 ID=FC930974; Name=FC930974; organism=Citrus clementina; type=EST; length=513bp
GGACTCCTGAGTTGATGCCTTTGAGTCATGTGCTTGCAACAAAGCTTGGT
GCTAAGCTTACAGAAGTGAGGAAGAATGGGACCTGTCCGTGGCTGAGGCC
AGATGGCAAAACACAAGTCACTGTTGAGTATTACAATGAGAATGGCGCCA
TGGTTCCTGTTCGTGTCCATACTGTGCTCATCTCAACTCAGCACGATGAG
ACTGTAACTAATGATGAGATTGCTGCAGATCTCAAGCAACATGTGATCAA
GCCTGTGATTCCTGACAAGTACTTGGATGACAAAACTATTTTCCATCTTA
ACCCATCTGGCCGTTTTGTGATTGGTGGCCCTCACGGTGATGCTGGGCTC
ACCGGCCGGAAGATTATTATTGATACTTATGGTGGCTGGNGGGCACACGG
TGGTGGGGCTTTCTCAGGGAAAGATCCTACCAAGGTTGATAGAAGCGGTG
CTTACATTGTAAGGCAAGCTGCTAAGAGTATTGTGGCAAGCGGGCTTGCC
AGGAGATGCATTG
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