FC931374

Overview
NameFC931374
Unique NameFC931374
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length736
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCD_ECOLI (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli (strain K12) GN=sucD PE=1 SV=2)

HSP 1 Score: 128.257 bits (321), Expect = 8.960e-46
Identity = 64/110 (58.18%), Postives = 77/110 (70.00%), Query Frame = 1 
Query:  370 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            +G T HLGLPVFN+V EA A T A ASVIYVP PF            + L++ ITEGIP  DM+ VK  L+ ++  R+IGPNCPG+I PGECKIGI PG+IHKPG+ GIV
Sbjct:   43 KGGTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPTLDMLTVKVKLD-EAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIV 151

HSP 2 Score: 77.0258 bits (188), Expect = 8.960e-46
Identity = 33/44 (75.00%), Postives = 38/44 (86.36%), Query Frame = 2 
Query:  248 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            ++ +DKNT+VICQG TG  GTFH+EQAI YGTKMVGGVTP KGG
Sbjct:    2 SILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGG 45
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCD_ECOL6 (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O6 GN=sucD PE=3 SV=2)

HSP 1 Score: 128.257 bits (321), Expect = 8.960e-46
Identity = 64/110 (58.18%), Postives = 77/110 (70.00%), Query Frame = 1 
Query:  370 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            +G T HLGLPVFN+V EA A T A ASVIYVP PF            + L++ ITEGIP  DM+ VK  L+ ++  R+IGPNCPG+I PGECKIGI PG+IHKPG+ GIV
Sbjct:   43 KGGTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPTLDMLTVKVKLD-EAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIV 151

HSP 2 Score: 77.0258 bits (188), Expect = 8.960e-46
Identity = 33/44 (75.00%), Postives = 38/44 (86.36%), Query Frame = 2 
Query:  248 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            ++ +DKNT+VICQG TG  GTFH+EQAI YGTKMVGGVTP KGG
Sbjct:    2 SILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGG 45
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCD_ECO57 (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O157:H7 GN=sucD PE=3 SV=2)

HSP 1 Score: 128.257 bits (321), Expect = 8.960e-46
Identity = 64/110 (58.18%), Postives = 77/110 (70.00%), Query Frame = 1 
Query:  370 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            +G T HLGLPVFN+V EA A T A ASVIYVP PF            + L++ ITEGIP  DM+ VK  L+ ++  R+IGPNCPG+I PGECKIGI PG+IHKPG+ GIV
Sbjct:   43 KGGTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPTLDMLTVKVKLD-EAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIV 151

HSP 2 Score: 77.0258 bits (188), Expect = 8.960e-46
Identity = 33/44 (75.00%), Postives = 38/44 (86.36%), Query Frame = 2 
Query:  248 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            ++ +DKNT+VICQG TG  GTFH+EQAI YGTKMVGGVTP KGG
Sbjct:    2 SILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGG 45
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCA_SCHPO (Probable succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial OS=Schizosaccharomyces pombe GN=SPAC16E8.17c PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 6.190e-44
Identity = 65/116 (56.03%), Postives = 81/116 (69.83%), Query Frame = 1 
Query:  352 GWRDAEEGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            G  + ++  T HLG PVF ++ EA  ETKA+AS ++VPPP             + L+V ITEGIPQHDM+RV   L  QSK+RL+GPNCPGII+PG+CKIGIMP +IHKPG  GIV
Sbjct:   69 GGTNPKKAGTTHLGKPVFGTIEEAMKETKADASAVFVPPPLAAGAIEEAIAAEVPLIVAITEGIPQHDMLRVSDILKTQSKSRLVGPNCPGIIRPGQCKIGIMPSHIHKPGCIGIV 184

HSP 2 Score: 61.6178 bits (148), Expect = 6.190e-44
Identity = 25/45 (55.56%), Postives = 34/45 (75.56%), Query Frame = 2 
Query:  245 PAVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            P + ++ +T+VI QG TGK GTFH + A++YGTK+VGG  PKK G
Sbjct:   33 PNLMINSDTKVIFQGFTGKQGTFHAQHAMDYGTKVVGGTNPKKAG 77
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCD_PSEAE (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2)

HSP 1 Score: 125.561 bits (314), Expect = 6.199e-44
Identity = 64/110 (58.18%), Postives = 74/110 (67.27%), Query Frame = 1 
Query:  370 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            +G T HLGLPVFN+V EA   T A ASVIYVP PF            + L+VCITEGIP  DM+  K   + +   RLIGPNCPG+I PGECKIGIMPG+IH PG+ GIV
Sbjct:   43 KGGTTHLGLPVFNTVKEAVEATGAEASVIYVPAPFCKDSILEAAFGGIKLIVCITEGIPTLDMLDAKVKCD-ELGVRLIGPNCPGVITPGECKIGIMPGHIHLPGKVGIV 151

HSP 2 Score: 73.559 bits (179), Expect = 6.199e-44
Identity = 32/44 (72.73%), Postives = 38/44 (86.36%), Query Frame = 2 
Query:  248 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            +V ++K+T+VICQG TG  GTFH+EQAI YGTKMVGGVTP KGG
Sbjct:    2 SVLINKDTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGG 45
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCD_BACSU (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Bacillus subtilis GN=sucD PE=1 SV=3)

HSP 1 Score: 130.183 bits (326), Expect = 3.955e-43
Identity = 68/110 (61.82%), Postives = 77/110 (70.00%), Query Frame = 1 
Query:  370 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            +G TE  G+PVFN+VAEA   T ANASVIYVP PF            LDLV+CITE IP  DMV+VK  +  + KTRLIGPNCPG+I P ECKIGIMPGYIHK G  G+V
Sbjct:   43 KGGTEAEGVPVFNTVAEAVQTTGANASVIYVPAPFAADAIMEAVDAELDLVICITEHIPVLDMVKVKRFMEGK-KTRLIGPNCPGVITPEECKIGIMPGYIHKKGHVGVV 151

HSP 2 Score: 66.2402 bits (160), Expect = 3.955e-43
Identity = 29/44 (65.91%), Postives = 36/44 (81.82%), Query Frame = 2 
Query:  248 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            +VF++K+TRVI QGITG    FHT+Q +EYGT +VGGVTP KGG
Sbjct:    2 SVFINKDTRVIVQGITGSTALFHTKQMLEYGTNIVGGVTPGKGG 45
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCA_CAEEL (Probable succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial OS=Caenorhabditis elegans GN=C05G5.4 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.485e-42
Identity = 70/116 (60.34%), Postives = 84/116 (72.41%), Query Frame = 1 
Query:  352 GWRDAEEGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            G  +A +  TEHLGLPVF +V+EA+ +T A+ASVIYVP               + LVVCITEGIPQHDMVRVK+ L +Q+KTRL+GPNCPGII   +CKIGIMPG+IHK G  GIV
Sbjct:   62 GGVNANKAGTEHLGLPVFKNVSEARNKTGADASVIYVPASAAGSAIEEAMDAEIPLVVCITEGIPQHDMVRVKSRLLKQNKTRLVGPNCPGIISADQCKIGIMPGHIHKRGCIGIV 177

HSP 2 Score: 56.225 bits (134), Expect = 1.485e-42
Identity = 24/41 (58.54%), Postives = 31/41 (75.61%), Query Frame = 2 
Query:  257 VDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            ++K+T+VI QG TGK GTFH +Q +EY TK+VGGV   K G
Sbjct:   30 INKDTKVIVQGFTGKQGTFHGKQMLEYNTKVVGGVNANKAG 70
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCD_HAEIN (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Haemophilus influenzae GN=sucD PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 7.277e-42
Identity = 60/110 (54.55%), Postives = 73/110 (66.36%), Query Frame = 1 
Query:  370 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            +G T HLGLPVFN+V EA   T   A+VIYVP  F            + L+VCITEGIP  DM++VK  LN ++   +IGPNCPG+I P ECKIGIMP +IHK G+ GIV
Sbjct:   43 KGGTTHLGLPVFNTVREAVENTGVTATVIYVPASFCKDAIIEAIDAGIQLIVCITEGIPTLDMLKVKQKLN-ETGVVMIGPNCPGVITPDECKIGIMPAHIHKKGKVGIV 151

HSP 2 Score: 73.1738 bits (178), Expect = 7.277e-42
Identity = 30/44 (68.18%), Postives = 38/44 (86.36%), Query Frame = 2 
Query:  248 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            A+ +DKNT+VICQG TG  GTFH+EQA+ YGT++VGGV+P KGG
Sbjct:    2 AILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKGG 45
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCD_STAAW (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain MW2) GN=sucD PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 1.026e-40
Identity = 62/103 (60.19%), Postives = 71/103 (68.93%), Query Frame = 1 
Query:  391 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            G+PVFN+V EAK ET A  SVIYVP PF            LD+V+CITE IP  DMV+VK  L  + KTRL+GPNCPG+I   ECKIGIMPGYIHK G  G+V
Sbjct:   50 GVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVKRYLQGR-KTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVV 151

HSP 2 Score: 66.2402 bits (160), Expect = 1.026e-40
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 2 
Query:  248 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            +VF+DKNT+V+ QGITG    FHT+Q ++YGTK+V GVTP KGG
Sbjct:    2 SVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKGG 45
[Close]
BLAST of FC931374 vs. ExPASy Swiss-Prot
Match: SUCD_STAAS (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain MSSA476) GN=sucD PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 1.026e-40
Identity = 62/103 (60.19%), Postives = 71/103 (68.93%), Query Frame = 1 
Query:  391 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRGGIV 699
            G+PVFN+V EAK ET A  SVIYVP PF            LD+V+CITE IP  DMV+VK  L  + KTRL+GPNCPG+I   ECKIGIMPGYIHK G  G+V
Sbjct:   50 GVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVKRYLQGR-KTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVV 151

HSP 2 Score: 66.2402 bits (160), Expect = 1.026e-40
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 2 
Query:  248 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 379
            +VF+DKNT+V+ QGITG    FHT+Q ++YGTK+V GVTP KGG
Sbjct:    2 SVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKGG 45
The following BLAST results are available for this feature:
BLAST of FC931374 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 46
Match NameE-valueIdentityDescription
SUCD_ECOLI8.960e-4658.18Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_ECOL68.960e-4658.18Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_ECO578.960e-4658.18Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCA_SCHPO6.190e-4456.03Probable succinyl-CoA ligase [GDP-forming] subunit... [more]
SUCD_PSEAE6.199e-4458.18Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_BACSU3.955e-4361.82Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCA_CAEEL1.485e-4260.34Probable succinyl-CoA ligase [GDP-forming] subunit... [more]
SUCD_HAEIN7.277e-4254.55Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAAW1.026e-4060.19Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAAS1.026e-4060.19Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]

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Properties
Property NameValue
Genbank descriptionC34104D11EF PostHarvP1 Citrus clementina cDNA clone C34104D11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931374 ID=FC931374; Name=FC931374; organism=Citrus clementina; type=EST; length=736bp
GGAGAATCTTTTTCTTCAATATATTTTGGGAAAAAAGAGAGAGACGAACT
CGAGGGAGAAAATCAGCGAATCAGATTAAAGTGAAGTGAATCAAATGGCT
CGCCAAGCCTTGGCCAAGCTGATCGGCTCGATCGGATGTCGCAGACCCTC
TACTGCTTCTTCCGTTTCACATTACAGATTATCAACGTCGGCGGCGCCGG
CGGCCCACTCTCGATATTATGGGTCGTCGGCGGCGGCGTCTCAGCCGGCA
GTTTTCGTCGACAAAAACACTCGAGTTATATGCCAAGGCATCACCGGAAA
AAACGGCACGTTCCATACCGAACAAGCCATCGAATACGGCACCAAAATGG
TGGGTGGCGTGACGCCGAAGAAGGGTGGACTGAGCACTTGGGACTTCCTG
TATTCAACTCGGTTGCTGAAGCTAAGGCTGAAACGAAGGCTAATGCTTCT
GTCATTTATGTTCCTCCGCCATTTGCTGCTGCTGCTATCATGGAGGCTAT
GGAGGCTGAGTTGGATCTTGTCGTCTGTATCACCGAAGGAATCCCTCAAC
ACGATATGGTGCGAGTGAAAGCTGCTCTAAACAGGCAATCTAAAACTCGG
CTGATTGGTCCCAACTGCCCTGGTATCATCAAACCTGGAGAATGTAAGAT
TGGCATTATGCCTGGGTATATTCACAAACCTGGGCGGGGTGGAATTGTTC
TCGATCTGGTACCTTGACTACGAGCAGTTTTCAACA
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