BQ622995

Overview
NameBQ622995
Unique NameBQ622995
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length660
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_ASPOR (Elongation factor 1-alpha OS=Aspergillus oryzae GN=tef1 PE=3 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 1.817e-49
Identity = 90/128 (70.31%), Postives = 114/128 (89.06%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEK 386
            +VK+++RG VA +SK+DP     +F +QVI++NHPGQ+GNGYAPVLDCHT+HIA KF+EL+ KIDRR+GK +E +PKF+K+GDA +VKMIP+KPM VE+F+D+PPLGRFAVRDMRQTVAVGVIKSVEK
Sbjct:  315 SVKEVRRGNVAGDSKNDPPAGCDSFNAQVIVLNHPGQVGNGYAPVLDCHTAHIACKFAELLEKIDRRTGKSVEDKPKFIKSGDAAIVKMIPSKPMCVESFTDFPPLGRFAVRDMRQTVAVGVIKSVEK 442          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_PODAN (Elongation factor 1-alpha OS=Podospora anserina GN=TEF PE=3 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 2.372e-49
Identity = 90/128 (70.31%), Postives = 113/128 (88.28%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEK 386
            +VK+++RG VA +SK+DP   AA+F +QVI++NHPGQ+G GYAPVLDCHT+HIA KFSEL+ KIDRR+GK +E+ PKF+K+GDA +VKM+P+KPM VE F++YPPLGRFAVRDMRQTVAVGVIK VEK
Sbjct:  315 SVKEIRRGNVAGDSKNDPPMGAASFDAQVIVLNHPGQVGAGYAPVLDCHTAHIACKFSELLQKIDRRTGKAVEESPKFIKSGDAAIVKMVPSKPMCVEAFTEYPPLGRFAVRDMRQTVAVGVIKKVEK 442          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A2_RAT (Elongation factor 1-alpha 2 OS=Rattus norvegicus GN=Eef1a2 PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 2.372e-49
Identity = 94/129 (72.87%), Postives = 111/129 (86.05%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 389
            +VKD++RG V  +SK DP +EAA FTSQVII+NHPGQI  GY+PV+DCHT+HIA KF+EL  KIDRRSGK+LE  PK LK+GDA +V+M+P KPM VE+FS YPPLGRFAVRDMRQTVAVGVIK+VEKK
Sbjct:  316 SVKDIRRGNVCGDSKADPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKK 444          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A2_MOUSE (Elongation factor 1-alpha 2 OS=Mus musculus GN=Eef1a2 PE=1 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 2.372e-49
Identity = 94/129 (72.87%), Postives = 111/129 (86.05%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 389
            +VKD++RG V  +SK DP +EAA FTSQVII+NHPGQI  GY+PV+DCHT+HIA KF+EL  KIDRRSGK+LE  PK LK+GDA +V+M+P KPM VE+FS YPPLGRFAVRDMRQTVAVGVIK+VEKK
Sbjct:  316 SVKDIRRGNVCGDSKADPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKK 444          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_PUCGR (Elongation factor 1-alpha OS=Puccinia graminis GN=TEF PE=3 SV=2)

HSP 1 Score: 194.897 bits (494), Expect = 3.099e-49
Identity = 92/130 (70.77%), Postives = 111/130 (85.38%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKD 392
            +VKD++RG V  ++K DP +EAA+F +QVI++NHPGQIGNGY PVLDCHT+HIA KF  L  KIDRR+GK LE+ PKF+K+GDA +VKM+P+K M VE F+DYPPLGRFAVRDMRQTVAVGVIKSVEK D
Sbjct:  313 SVKDIRRGNVCGDTKCDPPKEAASFVAQVIVLNHPGQIGNGYCPVLDCHTAHIACKFDTLQQKIDRRTGKALEEAPKFVKSGDACLVKMVPSKAMCVEPFADYPPLGRFAVRDMRQTVAVGVIKSVEKTD 442          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_PODCU (Elongation factor 1-alpha OS=Podospora curvicolla GN=TEF PE=3 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 5.285e-49
Identity = 90/128 (70.31%), Postives = 113/128 (88.28%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEK 386
            +VK+++RG VA +SK+DP   AA+F +QVI++NHPGQ+G GYAPVLDCHT+HIA KF+EL+ KIDRR+GK +E+ PKF+K+GDA +VKMIP+KPM VE F++YPPLGRFAVRDMRQTVAVGVIK VEK
Sbjct:  315 SVKEIRRGNVAGDSKNDPPLGAASFDAQVIVLNHPGQVGAGYAPVLDCHTAHIACKFAELLQKIDRRTGKAVEESPKFIKSGDAAIVKMIPSKPMCVEAFTEYPPLGRFAVRDMRQTVAVGVIKKVEK 442          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_ARXAD (Elongation factor 1-alpha OS=Arxula adeninivorans GN=TEF PE=3 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 5.285e-49
Identity = 91/130 (70.00%), Postives = 112/130 (86.15%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKD 392
            +VK+++RG VA +SK+DP +   +FT+QVII NHPGQI  GY+PVLDCHT+HIA +F EL+ KIDRRSGK++E  PKF+K GDA +V+MIP+KPM VETF++YPPLGRFAVRDMRQTVAVGVIKSVEK D
Sbjct:  314 SVKEIRRGNVAGDSKNDPPKGCDSFTAQVIIFNHPGQISAGYSPVLDCHTAHIACRFDELIEKIDRRSGKKVEDSPKFVKAGDAAIVRMIPSKPMCVETFTEYPPLGRFAVRDMRQTVAVGVIKSVEKSD 443          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_PICGU (Elongation factor 1-alpha OS=Pichia guilliermondii GN=TEF1 PE=3 SV=2)

HSP 1 Score: 193.741 bits (491), Expect = 6.903e-49
Identity = 89/133 (66.92%), Postives = 112/133 (84.21%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VK+++RG V  +SK+DP +   +FT+QVI++NHPGQI  GY+PVLDCHT+HIA KF  L+ KIDRR+GK++E  PKF+K+GDA +VKM+P+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIKSVEK D  G
Sbjct:  314 SVKEIRRGNVCGDSKNDPPKGCDSFTAQVIVLNHPGQISAGYSPVLDCHTAHIACKFDTLLEKIDRRTGKKMEDNPKFVKSGDASIVKMVPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVEKSDKAG 446          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_CRYNE (Elongation factor 1-alpha OS=Cryptococcus neoformans GN=TEF1 PE=2 SV=2)

HSP 1 Score: 192.971 bits (489), Expect = 1.177e-48
Identity = 88/133 (66.17%), Postives = 113/133 (84.96%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            ++KD++RG V  +SK+DP  EAA+F +QVI++NHPGQIG GY PVLDCHT+HIA KF+EL+ KIDRR+GK +E  PKF+K+GDA +VK++  KP+ VET++DYPPLGRFAVRDMRQTVAVGVIKSV+K +  G
Sbjct:  314 SIKDIRRGNVCGDSKNDPPMEAASFNAQVIVLNHPGQIGAGYTPVLDCHTAHIACKFAELIEKIDRRTGKVMEAAPKFVKSGDAAIVKLVAQKPLCVETYADYPPLGRFAVRDMRQTVAVGVIKSVDKTEKGG 446          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A2_SCHPO (Elongation factor 1-alpha-B/C OS=Schizosaccharomyces pombe GN=tef1b PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.008e-48
Identity = 90/131 (68.70%), Postives = 112/131 (85.50%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDP 395
            +VKD++RG V  +SK+DP    A+FT+QVII+NHPGQI  GY+PVLDCHT+HIA KF+EL+ KIDRRSGK++E+ PKF+K+GDA + KM+P+KPM VE F+DY PLGRFAVRDMRQTVAVGVIK+VEK  P
Sbjct:  314 SVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAP 444          
The following BLAST results are available for this feature:
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 171
Match NameE-valueIdentityDescription
EF1A_ASPOR1.817e-4970.31Elongation factor 1-alpha OS=Aspergillus oryzae GN... [more]
EF1A_PODAN2.372e-4970.31Elongation factor 1-alpha OS=Podospora anserina GN... [more]
EF1A2_RAT2.372e-4972.87Elongation factor 1-alpha 2 OS=Rattus norvegicus G... [more]
EF1A2_MOUSE2.372e-4972.87Elongation factor 1-alpha 2 OS=Mus musculus GN=Eef... [more]
EF1A_PUCGR3.099e-4970.77Elongation factor 1-alpha OS=Puccinia graminis GN=... [more]
EF1A_PODCU5.285e-4970.31Elongation factor 1-alpha OS=Podospora curvicolla ... [more]
EF1A_ARXAD5.285e-4970.00Elongation factor 1-alpha OS=Arxula adeninivorans ... [more]
EF1A_PICGU6.903e-4966.92Elongation factor 1-alpha OS=Pichia guilliermondii... [more]
EF1A_CRYNE1.177e-4866.17Elongation factor 1-alpha OS=Cryptococcus neoforma... [more]
EF1A2_SCHPO2.008e-4868.70Elongation factor 1-alpha-B/C OS=Schizosaccharomyc... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00086 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00086 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ622995 ID=BQ622995; Name=BQ622995; organism=Citrus sinensis; type=EST; length=660bp
TTGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCTTCCAACTCGAAGGAT
GATCCGGCCAGGGAAGCTGCCAACTTCACCTCACAGGTCATCATCATGAA
CCACCCTGGCCAGATTGGAAATGGATATGCCCCAGTGCTTGACTGCCACA
CCTCCCACATTGCCGTCAAGTTTTCTGAGCTCGTGACGAAGATTGATAGG
CGATCAGGCAAGGAGCTTGAGAAGGAGCCCAAGTTCTTGAAGAATGGAGA
CGCCGGAATGGTTAAGATGATTCCCACCAAGCCCATGGTTGTTGAAACCT
TCTCCGATTACCCACCCCTTGGTCGTTTTGCTGTTAGGGACATGCGTCAG
ACTGTTGCTGTGGGTGTTATCAAGAGTGTTGAGAAGAAGGATCCCACAGG
TGCGAAGGTCACCAAGTCCGCAGCCAAGAAGAAATGAGAAATTTTTATGG
GTCTGTCTACAATAGTTAGGGTTTATTTTCTGTCTTTTTTTATTTTCTTG
TCTGTTTTTGTTTTGCATCAATGTTTCTGCAAACAGCCCTATTTGCTTCC
ACCAGTTTGCTGTTGAGCAATTGGGATGTTATCTTTCTGCTTAACTGGCT
GTGGGTGGGTGTTCGGATTTTTATATTCTGAATTTGGTTAATAGCACTAA
ATTGAGTTGT
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