BQ623021

Overview
NameBQ623021
Unique NameBQ623021
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length526
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC9B_DANRE (SUMO-conjugating enzyme UBC9-B OS=Danio rerio GN=ube2ib PE=1 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 5.808e-14
Identity = 27/72 (37.50%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQ--WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  +G++CL IL+E   W PA+TI ++LL I  LL +PN  DP   E   +Y  ++ +YE   R+  +K++
Sbjct:   85 NVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFS 156

HSP 2 Score: 38.891 bits (89), Expect = 5.808e-14
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+ GG+F + + F  DYP  PPK  F   +FHP
Sbjct:   51 TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHP 84
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC12_DICDI (NEDD8-conjugating enzyme Ubc12 OS=Dictyostelium discoideum GN=ube2m PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 9.626e-14
Identity = 24/63 (38.10%), Postives = 38/63 (60.32%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTAR 298
            NI+  G +CL+IL++ W P L I  V+  + +L  +PNPDDPL  + A +   +K  +E+  R
Sbjct:  151 NIDLEGHVCLNILRQDWMPVLNIGTVIFGLMTLFLEPNPDDPLNKDAAQLMIDNKKTFEANVR 213

HSP 2 Score: 37.7354 bits (86), Expect = 9.626e-14
Identity = 16/36 (44.44%), Postives = 20/36 (55.56%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D  Y    F  TI+ P  YP+ PPKV   T V+HP
Sbjct:  115 TDGLYQSATFQFTINIPSTYPYDPPKVHCDTLVYHP 150
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC_ASFB7 (Ubiquitin-conjugating enzyme E2 OS=African swine fever virus (strain Badajoz 1971 Vero-adapted) GN=UBC PE=2 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 1.254e-13
Identity = 30/81 (37.04%), Postives = 42/81 (51.85%), Query Frame = 2 
Query:  110 NINSNGSICLDIL-----KEQ---WSPALTISKVLLSICSLLTDPNPDDPLVPEIA-----HMYKSDKAKYESTARSWTQK 313
            NI  +G +C+ IL     +EQ   WSPA  I  +LLS+ SLL +PNPD P   + A     ++YK D   Y    +   +K
Sbjct:   77 NIYPDGRLCISILHGDNAEEQGMTWSPAQKIDTILLSVISLLNEPNPDSPANVDAAKSYRKYVYKEDLESYPMEVKKTVKK 157

HSP 2 Score: 46.595 bits (109), Expect = 1.254e-13
Identity = 16/35 (45.71%), Postives = 26/35 (74.29%), Query Frame = 1 
Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            D+ Y GG+F   + FPP+YP+ PPK+ F ++++HP
Sbjct:   42 DTLYEGGLFKAKVAFPPEYPYAPPKLTFTSEMWHP 76
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROSI (Ubiquitin-conjugating enzyme E2 S OS=Drosophila simulans GN=GD17343 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.255e-13
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 43.8986 bits (102), Expect = 1.255e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA G+F V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAAGIFRVKLTLNKDFPLTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROSE (Ubiquitin-conjugating enzyme E2 S OS=Drosophila sechellia GN=GM13299 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.255e-13
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 43.8986 bits (102), Expect = 1.255e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA G+F V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAAGIFRVKLTLNKDFPLTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROME (Ubiquitin-conjugating enzyme E2 S OS=Drosophila melanogaster GN=CG8188 PE=1 SV=2)

HSP 1 Score: 51.9878 bits (123), Expect = 1.255e-13
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 43.8986 bits (102), Expect = 1.255e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA G+F V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAAGIFRVKLTLNKDFPLTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DANRE (Ubiquitin-conjugating enzyme E2 S OS=Danio rerio GN=ube2s PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.627e-13
Identity = 27/72 (37.50%), Postives = 38/72 (52.78%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAMG 325
            N+   G IC+++LK  W   L +  VLL+I  LL  PNP+  L  E   +   D  +Y S A   T+ +AMG
Sbjct:   87 NVGHKGEICVNVLKRDWKAELGLRHVLLTIKCLLIHPNPESALNEEAGKLLLEDYKEYASRAHLLTEIHAMG 158

HSP 2 Score: 41.5874 bits (96), Expect = 1.627e-13
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYAGGVF + +    D+P  PP+  F TK+FHP
Sbjct:   53 TPYAGGVFRMRLVLGKDFPAVPPRGYFLTKIFHP 86
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROVI (Ubiquitin-conjugating enzyme E2 S OS=Drosophila virilis GN=GJ15767 PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.632e-13
Identity = 28/70 (40.00%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ SNG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVASNGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 42.3578 bits (98), Expect = 1.632e-13
Identity = 18/34 (52.94%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA GVF V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAVGVFRVKLTLSKDFPQTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC11_SCHPO (Ubiquitin-conjugating enzyme E2-20 kDa OS=Schizosaccharomyces pombe GN=ubc11 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.643e-13
Identity = 24/59 (40.68%), Postives = 39/59 (66.10%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N++ +G+ICLDILK++WS    +  +LLS+ SLL +PN   PL  + A ++  D  +Y+
Sbjct:  105 NVDMSGNICLDILKDKWSAVYNVQTILLSLQSLLGEPNNASPLNAQAAELWSKDPIEYK 163

HSP 2 Score: 38.891 bits (89), Expect = 1.643e-13
Identity = 14/36 (38.89%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            SD+ Y G  F +++ FP +YP+ PP + F + ++HP
Sbjct:   69 SDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHP 104
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROYA (Ubiquitin-conjugating enzyme E2 S OS=Drosophila yakuba GN=GE15576 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 2.118e-13
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 43.1282 bits (100), Expect = 2.118e-13
Identity = 18/34 (52.94%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA GVF V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAAGVFRVKLTLNKDFPQTPPKAYFLTKIFHP 89
The following BLAST results are available for this feature:
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201
Match NameE-valueIdentityDescription
UBC9B_DANRE5.808e-1437.50SUMO-conjugating enzyme UBC9-B OS=Danio rerio GN=u... [more]
UBC12_DICDI9.626e-1438.10NEDD8-conjugating enzyme Ubc12 OS=Dictyostelium di... [more]
UBC_ASFB71.254e-1337.04Ubiquitin-conjugating enzyme E2 OS=African swine f... [more]
UBE2S_DROSI1.255e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila si... [more]
UBE2S_DROSE1.255e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila se... [more]
UBE2S_DROME1.255e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila me... [more]
UBE2S_DANRE1.627e-1337.50Ubiquitin-conjugating enzyme E2 S OS=Danio rerio G... [more]
UBE2S_DROVI1.632e-1340.00Ubiquitin-conjugating enzyme E2 S OS=Drosophila vi... [more]
UBC11_SCHPO1.643e-1340.68Ubiquitin-conjugating enzyme E2-20 kDa OS=Schizosa... [more]
UBE2S_DROYA2.118e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila ya... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUSDA-FP_00112 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00112 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623021 ID=BQ623021; Name=BQ623021; organism=Citrus sinensis; type=EST; length=526bp
TCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCC
TCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCT
TCCACCCTGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAA
AGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCT
GCTCCTTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATT
GCCCACATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAG
CTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGATATTGCAGAA
AAAGGAGCATTTATTGAAATATGTTGTGTGTGTTGTCTATCTATTTTCTT
TAACTTGCTTTGGGGAGGGCAGACGTTGGTGTTTTAGAATGGTGTTGGGA
GGAGTTGCAAGGGCGGGACGTTTTTATGTTAATGTTGAATGTTTGTGTTT
GAAGCTTCTTCGATGGAACGTAGTTA
back to top