BQ623067

Overview
NameBQ623067
Unique NameBQ623067
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length476
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: ADH1_GADCA (Alcohol dehydrogenase 1 OS=Gadus callarias PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.058e-22
Identity = 47/124 (37.90%), Postives = 78/124 (62.90%), Query Frame = 1 
Query:   43 VIKEMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILG-VEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLLLEGKSLRCVIWMD 411
            V+ +MT+GG D+  EC+G   VM +A  S  +GWG +V++G  ++H   ++   I+++ GR+  G+ FGG K K  +  + + YLDK++ LDEFITH +    +N A DL+  GK +R V+ ++
Sbjct:  254 VLSKMTNGGVDFSLECVGNVGVMRNALESCLKGWGVSVLVGWTDLHD--VATRPIQLIAGRTWKGSMFGGFKGKDGVPKMVKAYLDKKVKLDEFITHRMPLESVNDAIDLMKHGKCIRTVLSLE 375

HSP 2 Score: 24.2534 bits (51), Expect = 2.058e-22
Identity = 9/12 (75.00%), Postives = 11/12 (91.67%), Query Frame = 3 
Query:    3 IIGVDINPEKFE 38
            II VD+NP+KFE
Sbjct:  220 IIAVDLNPDKFE 231
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: FADH_PICPA (S-(hydroxymethyl)glutathione dehydrogenase OS=Pichia pastoris GN=FLD1 PE=3 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.080e-22
Identity = 50/119 (42.02%), Postives = 73/119 (61.34%), Query Frame = 1 
Query:   52 EMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILGVEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLLLEGKSLRCVIWM 408
            ++TDGG DY F+C G   VM +A  S  +GWG+++I+GV   G  IS    +++ GR   G  FGG+K ++ + +L Q YLD ++ +DEFITH     +INKAF  +  G  +R VI M
Sbjct:  260 DITDGGFDYTFDCTGNVQVMRNALESCHKGWGESIIIGVAAAGKEISTRPFQLVTGRVWRGCAFGGIKGRTQMPSLVQDYLDGKIKVDEFITHRHDLDNINKAFHDMHAGNCIRAVITM 378
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: FADH_YEAST (S-(hydroxymethyl)glutathione dehydrogenase OS=Saccharomyces cerevisiae GN=SFA1 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 6.052e-22
Identity = 49/122 (40.16%), Postives = 75/122 (61.48%), Query Frame = 1 
Query:   52 EMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILGVEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLLLEGKSLRCVIWMDKL 417
            EMTDGG D+ F+C G   +M DA  +  +GWG+++I+GV   G  IS    +++ GR   G+ FGG+K +S++  L + Y    L ++EFITH   F +IN+AF+ L  G  LR V+  D++
Sbjct:  264 EMTDGGLDFTFDCTGNTKIMRDALEACHKGWGQSIIIGVAAAGEEISTRPFQLVTGRVWKGSAFGGIKGRSEMGGLIKDYQKGALKVEEFITHRRPFKEINQAFEDLHNGDCLRTVLKSDEI 385
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: FRMA_ECOLI (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain K12) GN=frmA PE=1 SV=3)

HSP 1 Score: 102.834 bits (255), Expect = 7.673e-22
Identity = 49/125 (39.20%), Postives = 80/125 (64.00%), Query Frame = 1 
Query:   28 KNLR*VIKEMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILGVEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLLLEGKSLRCVI 402
            K ++ V+ ++   G D+ FECIG  +VM  A  S+  GWG++VI+GV + G  IS    +++ GR   G+ FGG+K +S +  + +  +  +++L+ F+TH +S  +IN AFDL+ EGKS+R VI
Sbjct:  243 KPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAVAGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVI 367

HSP 2 Score: 21.9422 bits (45), Expect = 7.673e-22
Identity = 7/13 (53.85%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IIGVDINPEKFEI 41
            II +D NP+KF++
Sbjct:  214 IIAIDTNPKKFDL 226
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: FRMA_ECOUT (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain UTI89 / UPEC) GN=frmA PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 7.673e-22
Identity = 49/125 (39.20%), Postives = 79/125 (63.20%), Query Frame = 1 
Query:   28 KNLR*VIKEMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILGVEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLLLEGKSLRCVI 402
            K ++ V+ ++   G D+ FECIG  +VM  A  S+  GWG++VI+GV   G  IS    +++ GR   G+ FGG+K +S +  + +  +  +++L+ F+TH +S  +IN AFDL+ EGKS+R VI
Sbjct:  243 KPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVI 367

HSP 2 Score: 23.0978 bits (48), Expect = 7.673e-22
Identity = 8/13 (61.54%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IIGVDINPEKFEI 41
            II +D NP+KFE+
Sbjct:  214 IIAIDTNPKKFEL 226
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: FRMA_ECOL6 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O6 GN=frmA PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 7.673e-22
Identity = 49/125 (39.20%), Postives = 79/125 (63.20%), Query Frame = 1 
Query:   28 KNLR*VIKEMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILGVEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLLLEGKSLRCVI 402
            K ++ V+ ++   G D+ FECIG  +VM  A  S+  GWG++VI+GV   G  IS    +++ GR   G+ FGG+K +S +  + +  +  +++L+ F+TH +S  +IN AFDL+ EGKS+R VI
Sbjct:  243 KPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVI 367

HSP 2 Score: 23.0978 bits (48), Expect = 7.673e-22
Identity = 8/13 (61.54%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IIGVDINPEKFEI 41
            II +D NP+KFE+
Sbjct:  214 IIAIDTNPKKFEL 226
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: FRMA_ECOL5 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=frmA PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 7.673e-22
Identity = 49/125 (39.20%), Postives = 79/125 (63.20%), Query Frame = 1 
Query:   28 KNLR*VIKEMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILGVEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLLLEGKSLRCVI 402
            K ++ V+ ++   G D+ FECIG  +VM  A  S+  GWG++VI+GV   G  IS    +++ GR   G+ FGG+K +S +  + +  +  +++L+ F+TH +S  +IN AFDL+ EGKS+R VI
Sbjct:  243 KPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVI 367

HSP 2 Score: 23.0978 bits (48), Expect = 7.673e-22
Identity = 8/13 (61.54%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IIGVDINPEKFEI 41
            II +D NP+KFE+
Sbjct:  214 IIAIDTNPKKFEL 226
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: FRMA_ECOK1 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O1:K1 / APEC GN=frmA PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 7.673e-22
Identity = 49/125 (39.20%), Postives = 79/125 (63.20%), Query Frame = 1 
Query:   28 KNLR*VIKEMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILGVEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLLLEGKSLRCVI 402
            K ++ V+ ++   G D+ FECIG  +VM  A  S+  GWG++VI+GV   G  IS    +++ GR   G+ FGG+K +S +  + +  +  +++L+ F+TH +S  +IN AFDL+ EGKS+R VI
Sbjct:  243 KPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVI 367

HSP 2 Score: 23.0978 bits (48), Expect = 7.673e-22
Identity = 8/13 (61.54%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IIGVDINPEKFEI 41
            II +D NP+KFE+
Sbjct:  214 IIAIDTNPKKFEL 226
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: ADH1_ZEALU (Alcohol dehydrogenase 1 (Fragment) OS=Zea luxurians GN=ADH1 PE=3 SV=2)

HSP 1 Score: 100.138 bits (248), Expect = 7.712e-22
Identity = 47/115 (40.87%), Postives = 71/115 (61.74%), Query Frame = 1 
Query:   28 KNLR*VIKEMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILGVEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLL 372
            K ++ V+ EMT+GG D   EC G  + M  AF    +GWG  V++GV    +    + +  L  R++ GTFFG  KP++D+  + + Y+ KEL +++FITH V F +INKAFDL+
Sbjct:  178 KPVQEVLAEMTNGGVDRSVECTGNINAMIQAFECVHDGWGVAVLVGVPHKDAEFKTHPMNFLNERTLKGTFFGNYKPRTDLPNVVELYMKKELEVEKFITHSVPFAEINKAFDLM 292

HSP 2 Score: 24.6386 bits (52), Expect = 7.712e-22
Identity = 9/12 (75.00%), Postives = 11/12 (91.67%), Query Frame = 3 
Query:    3 IIGVDINPEKFE 38
            IIGVD+NP +FE
Sbjct:  149 IIGVDLNPSRFE 160
[Close]
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Match: ADH4_STRCA (Alcohol dehydrogenase 4 OS=Struthio camelus GN=ADH4 PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 9.977e-22
Identity = 50/127 (39.37%), Postives = 74/127 (58.27%), Query Frame = 1 
Query:   22 ILKNLR*VIKEMTDGGADYCFECIGLASVMNDAFNSSREGWGKTVILGVEMHGSPISLNSIEILKGRSVCGTFFGGLKPKSDIATLAQKYLDKELNLDEFITHEVSFHDINKAFDLLLEGKSLRCVI 402
            I K ++ +I EMT+GG D+  ECIG   VM  AF S+  GWG  VI+GV +    I  + ++++ GR +  TFFGG K    +  L   Y+ K+ +LD  ++H +    IN AFDL+  GKS R ++
Sbjct:  251 IRKPVQEIIAEMTNGGVDFAIECIGNPDVMKAAFESTTVGWGTCVIVGVAVGEQSIPFSPMQLIMGRKIKATFFGGWKSVKSVPKLVSDYMAKKFDLDALVSHTLPLDKINDAFDLMNAGKSNRTIL 377

HSP 2 Score: 22.3274 bits (46), Expect = 9.977e-22
Identity = 8/16 (50.00%), Postives = 11/16 (68.75%), Query Frame = 3 
Query:    3 IIGVDINPEKFEIGDK 50
            I G+DIN +KF +  K
Sbjct:  224 IFGIDINKDKFPLAKK 239
The following BLAST results are available for this feature:
BLAST of BQ623067 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 111
Match NameE-valueIdentityDescription
ADH1_GADCA2.058e-2237.90Alcohol dehydrogenase 1 OS=Gadus callarias PE=1 SV... [more]
FADH_PICPA2.080e-2242.02S-(hydroxymethyl)glutathione dehydrogenase OS=Pich... [more]
FADH_YEAST6.052e-2240.16S-(hydroxymethyl)glutathione dehydrogenase OS=Sacc... [more]
FRMA_ECOLI7.673e-2239.20S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOUT7.673e-2239.20S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOL67.673e-2239.20S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOL57.673e-2239.20S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOK17.673e-2239.20S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
ADH1_ZEALU7.712e-2240.87Alcohol dehydrogenase 1 (Fragment) OS=Zea luxurian... [more]
ADH4_STRCA9.977e-2239.37Alcohol dehydrogenase 4 OS=Struthio camelus GN=ADH... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00158 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00158 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623067 ID=BQ623067; Name=BQ623067; organism=Citrus sinensis; type=EST; length=476bp
AGATTATAGGTGTTGATATAAATCCTGAAAAATTTGAGATAGGTGATAAA
GGAAATGACAGATGGGGGAGCAGACTATTGCTTCGAGTGCATTGGCTTGG
CGTCCGTAATGAATGATGCTTTCAACAGTAGCCGAGAGGGCTGGGGCAAG
ACGGTGATACTAGGAGTGGAGATGCATGGCTCCCCAATTTCTTTGAATTC
CATCGAGATTCTCAAAGGCAGAAGCGTTTGTGGCACATTTTTCGGAGGGC
TGAAACCCAAATCTGACATTGCTACTCTCGCCCAGAAGTACCTAGACAAG
GAGCTTAATCTGGACGAGTTCATAACACACGAAGTGAGCTTCCACGATAT
TAACAAAGCTTTCGACTTGCTCCTCGAAGGAAAAAGTCTGCGTTGCGTCA
TCTGGATGGATAAATTGATATAGGACAGCAAGTATTCTTCTTATATGGTA
CACTTTATTGAAATAATCAAGTTTGG
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