BQ623101

Overview
NameBQ623101
Unique NameBQ623101
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length646
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12771 contig Csv1_Contig12771:132..777. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_VIBC3 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 64/132 (48.48%), Postives = 88/132 (66.67%), Query Frame = 3 
Query:  246 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            R+GHGFD+HR     P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA+   DIG+ FPD+D KWKGA S   +K+  R +   GY +GN D T+I Q PK++PY + +   ++E L  D    N+KA T
Sbjct:    3 RIGHGFDVHRFGGEGPIIIGGVKIPYEQGLIAHSDGDVALHALSDALLGAIAAGDIGRHFPDTDDKWKGADSRELLKDVYRRVKAQGYVLGNADVTIIAQAPKMAPYIQAMCAAIAEDLETDLGNINVKATT 134
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHISS (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella sonnei (strain Ss046) GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 64/132 (48.48%), Postives = 89/132 (67.42%), Query Frame = 3 
Query:  246 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG      N+KA T
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLGCHMDDVNVKATT 133
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHIFL (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella flexneri GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 64/132 (48.48%), Postives = 89/132 (67.42%), Query Frame = 3 
Query:  246 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG      N+KA T
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLGCHMDDVNVKATT 133
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHIF8 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella flexneri serotype 5b (strain 8401) GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 64/132 (48.48%), Postives = 89/132 (67.42%), Query Frame = 3 
Query:  246 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG      N+KA T
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLGCHMDDVNVKATT 133
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHIDS (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 64/132 (48.48%), Postives = 89/132 (67.42%), Query Frame = 3 
Query:  246 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG      N+KA T
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLGCHMDDVNVKATT 133
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHIBS (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella boydii serotype 4 (strain Sb227) GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 64/132 (48.48%), Postives = 89/132 (67.42%), Query Frame = 3 
Query:  246 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG      N+KA T
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLGCHMDDVNVKATT 133
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHIB3 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 64/132 (48.48%), Postives = 89/132 (67.42%), Query Frame = 3 
Query:  246 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG      N+KA T
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLGCHMDDVNVKATT 133
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHESR (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shewanella sp. (strain MR-7) GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 66/135 (48.89%), Postives = 90/135 (66.67%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            +  R+GHGFD+H+     PLI+ G+ VP+E G  AHSDGDV+LH + DAILGA+ L DIG+ FPD+D  +KGA S V ++    L    G+E+GNLD T+I Q PK++P+ E +R  L+  L AD    N+KA T
Sbjct:    1 MKIRIGHGFDVHKFGEARPLILCGVEVPYETGLVAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYKGADSRVLLRHCYALARAKGFELGNLDVTIIAQAPKMAPHIEDMRQVLAADLNADIADINVKATT 135
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHESM (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shewanella sp. (strain MR-4) GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 66/135 (48.89%), Postives = 90/135 (66.67%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            +  R+GHGFD+H+     PLI+ G+ VP+E G  AHSDGDV+LH + DAILGA+ L DIG+ FPD+D  +KGA S V ++    L    G+E+GNLD T+I Q PK++P+ E +R  L+  L AD    N+KA T
Sbjct:    1 MKIRIGHGFDVHKFGEARPLILCGVEVPYETGLVAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYKGADSRVLLRHCYALARAKGFELGNLDVTIIAQAPKMAPHIEDMRQVLAADLNADIADINVKATT 135
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHESA (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shewanella sp. (strain ANA-3) GN=ispF PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.156e-31
Identity = 66/135 (48.89%), Postives = 90/135 (66.67%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            +  R+GHGFD+H+     PLI+ G+ VP+E G  AHSDGDV+LH + DAILGA+ L DIG+ FPD+D  +KGA S V ++    L    G+E+GNLD T+I Q PK++P+ E +R  L+  L AD    N+KA T
Sbjct:    1 MKIRIGHGFDVHKFGEARPLILCGVEVPYETGLVAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYKGADSRVLLRHCYALARAKGFELGNLDVTIIAQAPKMAPHIEDMRQVLAADLNADIADINVKATT 135
The following BLAST results are available for this feature:
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ISPF_VIBC32.156e-3148.482-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHISS2.156e-3148.482-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHIFL2.156e-3148.482-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHIF82.156e-3148.482-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHIDS2.156e-3148.482-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHIBS2.156e-3148.482-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHIB32.156e-3148.482-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHESR2.156e-3148.892-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHESM2.156e-3148.892-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHESA2.156e-3148.892-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00192 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00192 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623101 ID=BQ623101; Name=BQ623101; organism=Citrus sinensis; type=EST; length=646bp
AGATGGTGTTGACGATGGCCGCTCAATCATACACCACCACTCCTCTCCAC
AGCAAAATTACGAATAAGCCACTATGTCCTCCTCCGTTGAGTTTGAAGCC
TCGTAGCTTAACCGCCAAACATCTCCGCACAACACAGTCAACGTCGCTGC
CTCGTATTTCAGTTTCAGCTGCAGCCATTTCTTCCATCGAAGTTAAAGAG
TCATCGGCTTCGATCCAACCGTCCAAATCAAAATCACTGCCTTTTCGTGT
GGGCCACGGGTTTGATCTTCATAGATTGGAGCCTGGCTACCCTCTGATCA
TCGGTGGAATCAATGTTCCCCACGAGAGAGGCTGCGAGGCTCACTCTGAC
GGTGATGTTTTGCTTCACTGTGTTGTGGATGCAATTTTGGGAGCATTGGG
TCTTCCTGATATTGGTCAGATATTTCCTGATTCTGATCCTAAGTGGAAGG
GTGCACCATCCTCTGTTTTTATCAAAGAAGCTGTGAGACTCATGGATGAG
GCCGGCTATGAGATTGGCAACTTGGATGCCACGCTGATTCTTCAAAGACC
AAAATTGAGCCCATACAAGGAGACTATCCGAACCAACTTGTCTGAACTGC
TAGGAGCAGACCCCCCCGGTGGAAATCTGAAAGCGAAAACTCATGA
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