BQ623118

Overview
NameBQ623118
Unique NameBQ623118
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length580
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2114 contig Csv1_Contig2114:563..1141. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: DCEC_LISMO (Probable glutamate decarboxylase gamma OS=Listeria monocytogenes GN=lmo2434 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 2.092e-45
Identity = 82/165 (49.70%), Postives = 113/165 (68.48%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIVS 509
            I V  ILG T  G+F+D++LL++ +   N+       IH+D ASG    PF+ PEL WDFRL  V SIN SGHKYGLVY G+GW++W++KE LP+ELIF ++YLG   PT  +NFS+ +SQ+I QYY  +R GFEGYR + E   + A+ L + +EK+G F I++
Sbjct:  205 IGVVGILGITYTGKFDDIQLLDEKVEAYNETNEHQLVIHIDGASGAMFTPFVNPELPWDFRLKNVVSINTSGHKYGLVYPGVGWILWKDKEYLPKELIFEVSYLGGSMPTMAINFSRSASQIIGQYYNFLRYGFEGYREIHEKTKKTAIYLAKTVEKSGYFEIIN 369
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: DCEC_LISIN (Probable glutamate decarboxylase gamma OS=Listeria innocua GN=lin2528 PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.732e-45
Identity = 81/165 (49.09%), Postives = 112/165 (67.88%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIVS 509
            I +  ILG T  G+F+D+ LL++ +   N+       IH+D ASG    PF+ PEL WDFRL  V SIN SGHKYGLVY G+GW++W++KE LP+ELIF ++YLG   PT  +NFS+ +SQ+I QYY  +R GFEGYR + E   + A+ L + +EK+G F I++
Sbjct:  205 IGIVGILGITYTGKFDDIALLDEKVEAYNEANEHQLVIHIDGASGAMFTPFVNPELPWDFRLKNVVSINTSGHKYGLVYPGVGWILWKDKEYLPKELIFEVSYLGGSMPTMAINFSRSASQIIGQYYNFLRYGFEGYREIHEKTKKTALYLSKTVEKSGYFEIIN 369
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: GADB_DICDI (Glutamate decarboxylase B OS=Dictyostelium discoideum GN=gadB PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 1.148e-43
Identity = 83/173 (47.98%), Postives = 117/173 (67.63%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIVSKDEGGSVG 533
            I V   LG T   ++EDV  +++ L +  KE+G + PIHVDAASGGF+APF+  E+ WDFRLP VKSIN SGHK+GL   G+GWVVWR K+DL ++L+F++NYLG +  TF+LNFS+   Q+IAQYY  +R G  GY  + + C +  + + + L+K G F+++    G   G
Sbjct:  199 IGVIPTLGVTFTLQYEDVFSISNALDKFEKESGINIPIHVDAASGGFVAPFIQQEIIWDFRLPRVKSINASGHKFGLSPLGVGWVVWREKKDLHKDLVFNVNYLGGNMSTFSLNFSRPGGQIIAQYYNFLRHGRNGYTLIQDACAQQGIFIGKELKKMGIFDLIYDGTGALPG 371
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: DCEB_SHIFL (Glutamate decarboxylase beta OS=Shigella flexneri GN=gadB PE=2 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 2.785e-41
Identity = 81/172 (47.09%), Postives = 113/172 (65.70%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIV---SKDEG 521
            I V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P++ WDFRLP VKSI+ SGHK+GL   G GWV+WR++E LP+EL+F+++YLG    TF +NFS+ + QVIAQYY+ +RLG EGY  V    ++ A  L + + K G +  +     DEG
Sbjct:  203 IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG 374

HSP 2 Score: 20.7866 bits (42), Expect = 2.785e-41
Identity = 7/14 (50.00%), Postives = 10/14 (71.43%), Query Frame = 1 
Query:  517 KGVPLVAFSLKDNK 558
            +G+P V F LKD +
Sbjct:  373 EGIPAVCFKLKDGE 386
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: DCEB_ECOLI (Glutamate decarboxylase beta OS=Escherichia coli (strain K12) GN=gadB PE=1 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 2.785e-41
Identity = 81/172 (47.09%), Postives = 113/172 (65.70%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIV---SKDEG 521
            I V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P++ WDFRLP VKSI+ SGHK+GL   G GWV+WR++E LP+EL+F+++YLG    TF +NFS+ + QVIAQYY+ +RLG EGY  V    ++ A  L + + K G +  +     DEG
Sbjct:  203 IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG 374

HSP 2 Score: 20.7866 bits (42), Expect = 2.785e-41
Identity = 7/14 (50.00%), Postives = 10/14 (71.43%), Query Frame = 1 
Query:  517 KGVPLVAFSLKDNK 558
            +G+P V F LKD +
Sbjct:  373 EGIPAVCFKLKDGE 386
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: DCEB_ECOL6 (Glutamate decarboxylase beta OS=Escherichia coli O6 GN=gadB PE=2 SV=2)

HSP 1 Score: 167.933 bits (424), Expect = 2.785e-41
Identity = 81/172 (47.09%), Postives = 113/172 (65.70%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIV---SKDEG 521
            I V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P++ WDFRLP VKSI+ SGHK+GL   G GWV+WR++E LP+EL+F+++YLG    TF +NFS+ + QVIAQYY+ +RLG EGY  V    ++ A  L + + K G +  +     DEG
Sbjct:  203 IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG 374

HSP 2 Score: 20.7866 bits (42), Expect = 2.785e-41
Identity = 7/14 (50.00%), Postives = 10/14 (71.43%), Query Frame = 1 
Query:  517 KGVPLVAFSLKDNK 558
            +G+P V F LKD +
Sbjct:  373 EGIPAVCFKLKDGE 386
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: DCEB_ECO57 (Glutamate decarboxylase beta OS=Escherichia coli O157:H7 GN=gadB PE=2 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 2.785e-41
Identity = 81/172 (47.09%), Postives = 113/172 (65.70%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIV---SKDEG 521
            I V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P++ WDFRLP VKSI+ SGHK+GL   G GWV+WR++E LP+EL+F+++YLG    TF +NFS+ + QVIAQYY+ +RLG EGY  V    ++ A  L + + K G +  +     DEG
Sbjct:  203 IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG 374

HSP 2 Score: 20.7866 bits (42), Expect = 2.785e-41
Identity = 7/14 (50.00%), Postives = 10/14 (71.43%), Query Frame = 1 
Query:  517 KGVPLVAFSLKDNK 558
            +G+P V F LKD +
Sbjct:  373 EGIPAVCFKLKDGE 386
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: DCEA_SHIFL (Glutamate decarboxylase alpha OS=Shigella flexneri GN=gadA PE=2 SV=2)

HSP 1 Score: 167.933 bits (424), Expect = 2.785e-41
Identity = 81/172 (47.09%), Postives = 113/172 (65.70%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIV---SKDEG 521
            I V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P++ WDFRLP VKSI+ SGHK+GL   G GWV+WR++E LP+EL+F+++YLG    TF +NFS+ + QVIAQYY+ +RLG EGY  V    ++ A  L + + K G +  +     DEG
Sbjct:  203 IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEG 374

HSP 2 Score: 20.7866 bits (42), Expect = 2.785e-41
Identity = 7/14 (50.00%), Postives = 10/14 (71.43%), Query Frame = 1 
Query:  517 KGVPLVAFSLKDNK 558
            +G+P V F LKD +
Sbjct:  373 EGIPAVCFKLKDGE 386
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: DCEA_ECOLI (Glutamate decarboxylase alpha OS=Escherichia coli (strain K12) GN=gadA PE=1 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 2.785e-41
Identity = 81/172 (47.09%), Postives = 113/172 (65.70%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIV---SKDEG 521
            I V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P++ WDFRLP VKSI+ SGHK+GL   G GWV+WR++E LP+EL+F+++YLG    TF +NFS+ + QVIAQYY+ +RLG EGY  V    ++ A  L + + K G +  +     DEG
Sbjct:  203 IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG 374

HSP 2 Score: 20.7866 bits (42), Expect = 2.785e-41
Identity = 7/14 (50.00%), Postives = 10/14 (71.43%), Query Frame = 1 
Query:  517 KGVPLVAFSLKDNK 558
            +G+P V F LKD +
Sbjct:  373 EGIPAVCFKLKDGE 386
[Close]
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Match: DCEA_ECOL6 (Glutamate decarboxylase alpha OS=Escherichia coli O6 GN=gadA PE=1 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 2.785e-41
Identity = 81/172 (47.09%), Postives = 113/172 (65.70%), Query Frame = 3 
Query:   15 ICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGFEGYRNVMENCHENAMVLKEGLEKTGRFNIV---SKDEG 521
            I V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P++ WDFRLP VKSI+ SGHK+GL   G GWV+WR++E LP+EL+F+++YLG    TF +NFS+ + QVIAQYY+ +RLG EGY  V    ++ A  L + + K G +  +     DEG
Sbjct:  203 IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG 374

HSP 2 Score: 20.7866 bits (42), Expect = 2.785e-41
Identity = 7/14 (50.00%), Postives = 10/14 (71.43%), Query Frame = 1 
Query:  517 KGVPLVAFSLKDNK 558
            +G+P V F LKD +
Sbjct:  373 EGIPAVCFKLKDGE 386
The following BLAST results are available for this feature:
BLAST of BQ623118 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 38
Match NameE-valueIdentityDescription
DCEC_LISMO2.092e-4549.70Probable glutamate decarboxylase gamma OS=Listeria... [more]
DCEC_LISIN2.732e-4549.09Probable glutamate decarboxylase gamma OS=Listeria... [more]
GADB_DICDI1.148e-4347.98Glutamate decarboxylase B OS=Dictyostelium discoid... [more]
DCEB_SHIFL2.785e-4147.09Glutamate decarboxylase beta OS=Shigella flexneri ... [more]
DCEB_ECOLI2.785e-4147.09Glutamate decarboxylase beta OS=Escherichia coli (... [more]
DCEB_ECOL62.785e-4147.09Glutamate decarboxylase beta OS=Escherichia coli O... [more]
DCEB_ECO572.785e-4147.09Glutamate decarboxylase beta OS=Escherichia coli O... [more]
DCEA_SHIFL2.785e-4147.09Glutamate decarboxylase alpha OS=Shigella flexneri... [more]
DCEA_ECOLI2.785e-4147.09Glutamate decarboxylase alpha OS=Escherichia coli ... [more]
DCEA_ECOL62.785e-4147.09Glutamate decarboxylase alpha OS=Escherichia coli ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00209 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00209 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623118 ID=BQ623118; Name=BQ623118; organism=Citrus sinensis; type=EST; length=580bp
GGATGAAAACACCTATCTGTGTTGCTGCTATTCTGGGTTCTACTCTCAAT
GGAGAATTTGAAGATGTCAAGCTCTTGAACGACCTTTTGACAGAGAAGAA
CAAGGAAACAGGATGGGATACTCCAATTCATGTTGATGCTGCTAGTGGTG
GCTTCATTGCACCATTTTTGTACCCAGAGCTTGAATGGGACTTCCGCTTG
CCGCTGGTGAAGAGTATTAATGTTAGTGGCCACAAGTACGGGCTTGTGTA
TGCTGGTATTGGTTGGGTTGTTTGGAGAAACAAAGAAGACTTGCCTGAAG
AACTCATCTTCCACATCAATTACCTAGGAGCTGATCAACCCACCTTCACC
CTCAATTTTTCTAAAGGTTCTAGCCAAGTAATTGCTCAGTACTACCAACT
AATCCGCTTAGGTTTTGAGGGATACCGCAATGTGATGGAGAATTGTCACG
AAAATGCAATGGTGCTGAAAGAAGGACTAGAGAAAACCGGCCGCTTCAAC
ATTGTGTCAAAGGACGAAGGGGGTTCCGTTGGTGGCATTTTCTCTCAAGG
ACAACAAACGCGCACGATGAATTCGAAGTG
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