BQ623127

Overview
NameBQ623127
Unique NameBQ623127
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length610
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_BRUAB (Aquaporin Z OS=Brucella abortus GN=aqpZ PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 6.770e-16
Identity = 47/144 (32.64%), Postives = 68/144 (47.22%), Query Frame = 1 
Query:   82 VAYMVAQCLGAICGVGLVKAFMKHEYNSLGGG------ANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAA 495
            + Y VAQ LGAI    ++      +     GG            GY+  + L  EII T   +  +  +T     A        AP+ IGF + ++HL +IP+T T +NPARS G A ++ +  A    W+FWV P VGA+  A
Sbjct:   82 IPYWVAQVLGAIAAAAILYVIASGKDGFSAGGLASNGYGELSPGGYSMMAGLLIEIILTAFFIIIILGSTSSLAPAG------FAPIAIGFGLTLIHLVSIPVTNTSVNPARSTGVA-LFADRAALSQLWLFWVAPLVGAVIGA 218

HSP 2 Score: 28.4906 bits (62), Expect = 6.770e-16
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:    3 GISGGHINPAVTFG 44
            GISGGH NPAV+ G
Sbjct:   56 GISGGHFNPAVSLG 69
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_BRUA2 (Aquaporin Z OS=Brucella abortus (strain 2308) GN=aqpZ PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 6.770e-16
Identity = 47/144 (32.64%), Postives = 68/144 (47.22%), Query Frame = 1 
Query:   82 VAYMVAQCLGAICGVGLVKAFMKHEYNSLGGG------ANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAA 495
            + Y VAQ LGAI    ++      +     GG            GY+  + L  EII T   +  +  +T     A        AP+ IGF + ++HL +IP+T T +NPARS G A ++ +  A    W+FWV P VGA+  A
Sbjct:   82 IPYWVAQVLGAIAAAAILYVIASGKDGFSAGGLASNGYGELSPGGYSMMAGLLIEIILTAFFIIIILGSTSSLAPAG------FAPIAIGFGLTLIHLVSIPVTNTSVNPARSTGVA-LFADRAALSQLWLFWVAPLVGAVIGA 218

HSP 2 Score: 28.4906 bits (62), Expect = 6.770e-16
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:    3 GISGGHINPAVTFG 44
            GISGGH NPAV+ G
Sbjct:   56 GISGGHFNPAVSLG 69
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQY1_YEAST (Aquaporin-1 OS=Saccharomyces cerevisiae GN=AQY1 PE=1 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 8.702e-16
Identity = 51/150 (34.00%), Postives = 74/150 (49.33%), Query Frame = 1 
Query:   64 VSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYI 513
            VS  R V   V+Q +  +   G   A    E       AN++  G ++   L  E+ GT +L  TV      KR         +A LPIG ++F+ H+A    TGTG+NPARS GAAV       +  HWI+W+GP +G++ A +  Q +
Sbjct:  131 VSPTRCVVMWVSQIVAGMAAGGAASAMTPGEVLF----ANSLGLGCSRTRGLFLEMFGTAILCLTVLMTAVEKRETN-----FMAALPIGISLFIAHVALTAYTGTGVNPARSLGAAVAARYFPHY--HWIYWIGPLLGSILAWSVWQLL 269

HSP 2 Score: 23.483 bits (49), Expect = 8.702e-16
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGK 59
            G+SGG +NPAV+    L +
Sbjct:  111 GVSGGALNPAVSLSLCLAR 129
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_PHOLL (Aquaporin Z OS=Photorhabdus luminescens subsp. laumondii GN=aqpZ PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 8.796e-16
Identity = 45/156 (28.85%), Postives = 78/156 (50.00%), Query Frame = 1 
Query:   73 IRAVAYMVAQCLGAICGVGLVKAFMKHE---------YNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYI 513
            +  + Y+++Q +G I    ++      +         + S G G ++   G++  SA+ AEI+ T + +  +  ATD +        P  APL IG A+ +++L +IPIT T +NPARS  A  I+ N  A +  W FWV P +G +     ++ +
Sbjct:   79 VEVIPYIISQVIGGILAAAVLYVIASGQVGFDATTSGFASNGFGEHS-PGGFSLQSAIVAEIVLTAIFLIVIIGATDRRAP------PGFAPLAIGLALVLINLISIPITNTSVNPARS-TAVAIFQNTWALEQLWFFWVMPIIGGIVGGGIYRLL 226

HSP 2 Score: 27.7202 bits (60), Expect = 8.796e-16
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    6 ISGGHINPAVTFG 44
            ISGGH NPAVT G
Sbjct:   57 ISGGHFNPAVTIG 69
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_BRUME (Aquaporin Z OS=Brucella melitensis GN=aqpZ PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 8.800e-16
Identity = 47/144 (32.64%), Postives = 68/144 (47.22%), Query Frame = 1 
Query:   82 VAYMVAQCLGAICGVGLVKAFMKHEYNSLGGG------ANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAA 495
            + Y VAQ LGAI    ++      +     GG            GY+  + L  EII T   +  +  +T     A        AP+ IGF + ++HL +IP+T T +NPARS G A ++ +  A    W+FWV P VGA+  A
Sbjct:   82 IPYWVAQVLGAIAAAAILYVIASGKDGFSAGGLASNGYGELSPGGYSMMAGLLIEIILTAFFIIIILGSTSSLAPAG------FAPIAIGFGLTLIHLVSIPVTNTWVNPARSTGVA-LFADTAALSQLWLFWVAPLVGAVIGA 218

HSP 2 Score: 28.4906 bits (62), Expect = 8.800e-16
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:    3 GISGGHINPAVTFG 44
            GISGGH NPAV+ G
Sbjct:   56 GISGGHFNPAVSLG 69
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: TIP1_TOBAC (Probable aquaporin TIP-type RB7-5A OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 8.805e-16
Identity = 52/140 (37.14%), Postives = 78/140 (55.71%), Query Frame = 1 
Query:   64 VSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFS-ATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVG 480
            ++++    Y +AQ LG+     L    +K+  N L    + VA+G N    +  EII TF LVYTV++ A DPK+ +    +  +AP+ IGF V    LA  P +G  +NPARSFG AV+  +   +  +WI+W GP +G
Sbjct:   96 ITILTGFFYWIAQLLGSTVACLL----LKYVTNGLAVPTHGVAAGLNGLQGVVMEIIITFALVYTVYATAADPKKGS----LGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVAGD---FSQNWIYWAGPLIG 224
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: TIP44_MAIZE (Aquaporin TIP4-4 OS=Zea mays GN=TIP4-4 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.140e-15
Identity = 52/151 (34.44%), Postives = 77/151 (50.99%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGA--EIIGTFVLVYTVFSAT-DPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGA-LAAAAY 501
            +++L R+  Y+ AQ LG+     L+ AF+         G    A G   G+  G   E + TF L++ V++   DP+R+     V  + PL +G  V    LA  P +G  +NPARSFG A++      W DHW++WVGP +G  LA   Y
Sbjct:   95 RITLFRSALYVAAQLLGSTLAC-LLLAFLA----VADSGVPVHALGAGVGALRGVLMEAVLTFSLLFAVYATVVDPRRA-----VGGMGPLLVGLVVGANVLAGGPFSGASMNPARSFGPALVAG---VWADHWVYWVGPLIGGPLAGLVY 232

HSP 2 Score: 28.8758 bits (63), Expect = 1.140e-15
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:    6 ISGGHINPAVTFG 44
            +SGGHINPAVT G
Sbjct:   77 VSGGHINPAVTLG 89
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_BORPA (Aquaporin Z OS=Bordetella parapertussis GN=aqpZ PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.143e-15
Identity = 48/150 (32.00%), Postives = 75/150 (50.00%), Query Frame = 1 
Query:   88 YMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGS--------ALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYI 513
            Y+VAQ +GAI     + +  +         +   A+GY   S        AL  EI+ +   V+ +  ATD +  A        AP+PIG A+ ++HL +IP+T T +NPARS G A ++    A +  W+FW+ P  GAL  A  ++ +
Sbjct:   87 YIVAQVVGAIVAAATLASIAQGVAGFDLVASKFAANGYGDHSPGKYSMQAALICEIVLSAGFVFVILGATDKRAPAG------FAPIPIGLALTLIHLISIPVTNTSVNPARSTGPA-LFVGGWALEQLWLFWLAPIAGALVGALAYRLV 229

HSP 2 Score: 27.335 bits (59), Expect = 1.143e-15
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    6 ISGGHINPAVTFG 44
            ISGGH NPAVT G
Sbjct:   60 ISGGHFNPAVTVG 72
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_BORBR (Aquaporin Z OS=Bordetella bronchiseptica GN=aqpZ PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.143e-15
Identity = 48/150 (32.00%), Postives = 75/150 (50.00%), Query Frame = 1 
Query:   88 YMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGS--------ALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYI 513
            Y+VAQ +GAI     + +  +         +   A+GY   S        AL  EI+ +   V+ +  ATD +  A        AP+PIG A+ ++HL +IP+T T +NPARS G A ++    A +  W+FW+ P  GAL  A  ++ +
Sbjct:   87 YIVAQVVGAIVAAATLASIAQGVAGFDLVASKFAANGYGDHSPGKYSMQAALICEIVLSAGFVFVILGATDKRAPAG------FAPIPIGLALTLIHLISIPVTNTSVNPARSTGPA-LFVGGWALEQLWLFWLAPIAGALVGALAYRLV 229

HSP 2 Score: 27.335 bits (59), Expect = 1.143e-15
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    6 ISGGHINPAVTFG 44
            ISGGH NPAVT G
Sbjct:   60 ISGGHFNPAVTVG 72
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_VIBPA (Aquaporin Z OS=Vibrio parahaemolyticus GN=aqpZ PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.486e-15
Identity = 48/151 (31.79%), Postives = 78/151 (51.66%), Query Frame = 1 
Query:   88 YMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGS--------ALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYIL 516
            Y++AQ +G +   G++      +      G+   A+GY + S        AL +EI+ T + +  +  ATD KR+ +       AP+ IG  + ++HL +IP+T T +NPARS  A  +Y  D A    W+FWV P VG +  A  ++ +L
Sbjct:   83 YIIAQVIGGLIAGGILYVIATGQAGFDVVGSGFAANGYGEHSPGQYSMLAALVSEIVMTMMFLIVIMGATD-KRAPQG-----FAPIAIGLCLTLIHLISIPVTNTSVNPARS-TAVAMYVGDWAVSQLWLFWVAPIVGGVLGAVIYKNLL 226

HSP 2 Score: 24.6386 bits (52), Expect = 1.486e-15
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    6 ISGGHINPAVTFG 44
            ISG H+NPAVT G
Sbjct:   56 ISGCHLNPAVTVG 68
The following BLAST results are available for this feature:
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 159
Match NameE-valueIdentityDescription
AQPZ_BRUAB6.770e-1632.64Aquaporin Z OS=Brucella abortus GN=aqpZ PE=3 SV=1[more]
AQPZ_BRUA26.770e-1632.64Aquaporin Z OS=Brucella abortus (strain 2308) GN=a... [more]
AQY1_YEAST8.702e-1634.00Aquaporin-1 OS=Saccharomyces cerevisiae GN=AQY1 PE... [more]
AQPZ_PHOLL8.796e-1628.85Aquaporin Z OS=Photorhabdus luminescens subsp. lau... [more]
AQPZ_BRUME8.800e-1632.64Aquaporin Z OS=Brucella melitensis GN=aqpZ PE=3 SV... [more]
TIP1_TOBAC8.805e-1637.14Probable aquaporin TIP-type RB7-5A OS=Nicotiana ta... [more]
TIP44_MAIZE1.140e-1534.44Aquaporin TIP4-4 OS=Zea mays GN=TIP4-4 PE=2 SV=1[more]
AQPZ_BORPA1.143e-1532.00Aquaporin Z OS=Bordetella parapertussis GN=aqpZ PE... [more]
AQPZ_BORBR1.143e-1532.00Aquaporin Z OS=Bordetella bronchiseptica GN=aqpZ P... [more]
AQPZ_VIBPA1.486e-1531.79Aquaporin Z OS=Vibrio parahaemolyticus GN=aqpZ PE=... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00218 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00218 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623127 ID=BQ623127; Name=BQ623127; organism=Citrus sinensis; type=EST; length=610bp
CTGGCATTTCTGGTGGTCACATTAACCCTGCTGTGACGTTTGGGCCTGTT
CTTGGCAAGGAAGGTATCATTGATCAGGGCTGTGGCTTACATGGTGGCTC
AGTGCTTGGGTGCCATTTGCGGTGTTGGTTTGGTCAAGGCCTTCATGAAG
CATGAGTACAACTCACTTGGTGGTGGTGCTAACACTGTGGCTTCTGGCTA
CAACAAAGGATCAGCCTTGGGTGCTGAGATTATTGGTACCTTCGTGCTTG
TTTACACAGTCTTCTCGGCCACAGACCCCAAGAGAAGCGCTCGTGACTCT
CACGTGCCCGTTTTGGCTCCACTTCCAATTGGATTTGCTGTGTTCATGGT
TCACTTGGCCACCATCCCCATCACTGGAACGGGCATCAACCCTGCTAGGA
GCTTTGGTGCTGCTGTTATCTACAACAATGACAAGGCCTGGGATGACCAT
TGGATCTTCTGGGTTGGTCCTTTCGTTGGAGCATTGGCTGCAGCGGCATA
CCACCAGTACATCTTGAGGGCTGCTGCTATCAAGGCTTTGGGATCATTCC
GCAACAACCCCTTCAAACTAAGAAAGCAAATTATGATGAATATGAACCAC
CAACCAGAAT
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