BQ623127

Overview
NameBQ623127
Unique NameBQ623127
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length610
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_ECOLI (Aquaporin Z OS=Escherichia coli (strain K12) GN=aqpZ PE=1 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 5.817e-14
Identity = 46/148 (31.08%), Postives = 70/148 (47.30%), Query Frame = 1 
Query:   82 VAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPK--RSARDSHVPV-LAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYIL 516
            V Y++AQ +G I    L+      +       +   ++GY + S  G  ++   V V  V SA        A D   P   AP+ IG A+ ++HL +IP+T T +NPARS  A  I+    A +  W FWV P VG +     ++ +L
Sbjct:   82 VGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALV-VELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARS-TAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLL 227

HSP 2 Score: 27.7202 bits (60), Expect = 5.817e-14
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    6 ISGGHINPAVTFG 44
            ISGGH NPAVT G
Sbjct:   57 ISGGHFNPAVTIG 69
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_ECOL6 (Aquaporin Z OS=Escherichia coli O6 GN=aqpZ PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 5.817e-14
Identity = 46/148 (31.08%), Postives = 70/148 (47.30%), Query Frame = 1 
Query:   82 VAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPK--RSARDSHVPV-LAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYIL 516
            V Y++AQ +G I    L+      +       +   ++GY + S  G  ++   V V  V SA        A D   P   AP+ IG A+ ++HL +IP+T T +NPARS  A  I+    A +  W FWV P VG +     ++ +L
Sbjct:   82 VGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALV-VELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARS-TAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLL 227

HSP 2 Score: 27.7202 bits (60), Expect = 5.817e-14
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    6 ISGGHINPAVTFG 44
            ISGGH NPAVT G
Sbjct:   57 ISGGHFNPAVTIG 69
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_ECO57 (Aquaporin Z OS=Escherichia coli O157:H7 GN=aqpZ PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 5.817e-14
Identity = 46/148 (31.08%), Postives = 70/148 (47.30%), Query Frame = 1 
Query:   82 VAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPK--RSARDSHVPV-LAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYIL 516
            V Y++AQ +G I    L+      +       +   ++GY + S  G  ++   V V  V SA        A D   P   AP+ IG A+ ++HL +IP+T T +NPARS  A  I+    A +  W FWV P VG +     ++ +L
Sbjct:   82 VGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALV-VELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARS-TAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLL 227

HSP 2 Score: 27.7202 bits (60), Expect = 5.817e-14
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    6 ISGGHINPAVTFG 44
            ISGGH NPAVT G
Sbjct:   57 ISGGHFNPAVTIG 69
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: TIP43_ORYSJ (Probable aquaporin TIP4-3 OS=Oryza sativa subsp. japonica GN=TIP4-3 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.076e-13
Identity = 54/152 (35.53%), Postives = 72/152 (47.37%), Query Frame = 1 
Query:   64 VSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTV-----ASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGA-LAAAAY 501
            ++L R+  Y  AQ LG+     L+ A        L GG   V     A G     A+  E + TF L++ V++    +R A    V  L PL +G  V    LA  P +G  +NPARSFG A+       W DHWI+WVGP +G  LA   Y
Sbjct:   95 ITLFRSALYAAAQLLGSSLACLLLAA--------LTGGEEAVPVHAPAPGVGAARAVAMEAVLTFSLLFAVYATVVDRRRA----VGALGPLLVGLVVGANILAGGPYSGASMNPARSFGPALAAGE---WADHWIYWVGPLIGGPLAGLVY 231
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: TIP11_ORYSJ (Probable aquaporin TIP1-1 OS=Oryza sativa subsp. japonica GN=TIP1-1 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.076e-13
Identity = 51/153 (33.33%), Postives = 79/153 (51.63%), Query Frame = 1 
Query:   64 VSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTV--ASGYNKGSALGAEIIGTFVLVYTVFS-ATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYI 513
            ++L R + Y +AQ LG+     L++        S GG A      +G +   AL  EI+ TF LVYTV++ A DPK+ +    +  +AP+ IGF V    L      G  +NPA SFG A++     +W+  W++WVGP +G   A   ++ +
Sbjct:   98 ITLFRGLLYWIAQLLGSTVACFLLRF-------STGGLATGTFGLTGVSVWEALVLEIVMTFGLVYTVYATAVDPKKGS----LGTIAPIAIGFIVGANILVGGAFDGASMNPAVSFGPALV---SWSWESQWVYWVGPLIGGGLAGVIYEVL 236
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: TIP43_MAIZE (Aquaporin TIP4-3 OS=Zea mays GN=TIP4-3 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.272e-13
Identity = 46/156 (29.49%), Postives = 72/156 (46.15%), Query Frame = 1 
Query:   64 VSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSAT-DPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAA 528
            V+L R+  Y+ AQ L +     L++         L    + +A G      + AE + TF L++ +++   DP++      +P   PL  G  V    +A   ++G  +NPARSFG AV       W  HW++WVGP  G   A   ++    AAA
Sbjct:   95 VTLFRSSLYIAAQMLASSAACFLLRWLT----GGLATPVHALAEGVGPLQGVVAEAVFTFSLLFVIYATILDPRKL-----LPGAGPLLTGLLVGANSVAGAALSGASMNPARSFGPAVASG---VWTHHWVYWVGPLAGGPLAVLVYECCFMAAA 238

HSP 2 Score: 28.1054 bits (61), Expect = 1.272e-13
Identity = 11/17 (64.71%), Postives = 13/17 (76.47%), Query Frame = 3 
Query:    6 ISGGHINPAVTFGPVLG 56
            ISGGH+NPAVT    +G
Sbjct:   76 ISGGHVNPAVTLSLAVG 92
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: AQPZ_BRAJA (Aquaporin Z OS=Bradyrhizobium japonicum GN=aqpZ PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.655e-13
Identity = 44/155 (28.39%), Postives = 72/155 (46.45%), Query Frame = 1 
Query:   88 YMVAQCLGAIC-------------GVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYI 513
            Y++AQ  GAI              G  + K F  + Y++   G       Y+  +    E++ T + ++ +  AT  +  A        APL IG A+ M+HL +IP+T T +NPARS G A ++    A    W+FWV P +G       ++++
Sbjct:   85 YVIAQVCGAIVAAELLYIIASGAPGFDVTKGFASNGYDAHSPGQ------YSMMACFLTEVVMTMMFLFIIMGATHGRAPAG------FAPLAIGLALVMIHLVSIPVTNTSVNPARSTGPA-LFVGGWAMAQLWLFWVAPLIGGALGGVIYRWL 226

HSP 2 Score: 25.409 bits (54), Expect = 1.655e-13
Identity = 11/17 (64.71%), Postives = 12/17 (70.59%), Query Frame = 3 
Query:    6 ISGGHINPAVTFGPVLG 56
            ISG H+NPAVT G   G
Sbjct:   58 ISGCHLNPAVTVGLAAG 74
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: TIP41_MAIZE (Aquaporin TIP4-1 OS=Zea mays GN=TIP4-1 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.145e-13
Identity = 50/157 (31.85%), Postives = 73/157 (46.50%), Query Frame = 1 
Query:   64 VSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKG----SALGAEIIGTFVLVYTVFSAT-DPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGA-LAAAAYHQYIL 516
            ++ +RAV Y+ AQ L +     L++         L GG  T      +G      L  E+I TF L++  ++   DP+     S V  + PL  G  V    LA    TG  +NPARSFG A+   +   W +HW++W+GP +G  LA   Y    L
Sbjct:  102 ITKLRAVLYVAAQLLASSAACVLLRF--------LSGGMVTPVHALGRGISPMQGLVMEVILTFSLLFVTYAMILDPR-----SQVRAIGPLLTGLIVGANSLAGGNFTGASMNPARSFGPALATGD---WTNHWVYWIGPLLGGPLAGFVYESLFL 242

HSP 2 Score: 27.7202 bits (60), Expect = 2.145e-13
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:    6 ISGGHINPAVTFG 44
            +SGGH+NPAVT G
Sbjct:   83 VSGGHLNPAVTVG 95
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: TIP51_ARATH (Probable aquaporin TIP5-1 OS=Arabidopsis thaliana GN=TIP5-1 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 2.786e-13
Identity = 44/153 (28.76%), Postives = 81/153 (52.94%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPV-LAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYIL 516
            ++S+  A+ Y  +Q + ++    ++K  +  ++  +      +A       A   E +  FVLVYTVF+A+DP+R      +P+ + P+ IGF      LA  P +G  +NPA +FG+A++Y + K   +  ++WVGP +G   AA  +  ++
Sbjct:   99 RISVPTAMFYWTSQMIASVMACLVLKVTVMEQHVPI----YKIAGEMTGFGASVLEGVLAFVLVYTVFTASDPRRG-----LPLAVGPIFIGFVAGANVLAAGPFSGGSMNPACAFGSAMVYGSFK---NQAVYWVGPLLGGATAALVYDNVV 239

HSP 2 Score: 28.4906 bits (62), Expect = 2.786e-13
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:    6 ISGGHINPAVTF 41
            +SGGH+NPAVTF
Sbjct:   81 VSGGHVNPAVTF 92
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: TIP22_MAIZE (Aquaporin TIP2-2 OS=Zea mays GN=TIP2-2 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.132e-13
Identity = 54/148 (36.49%), Postives = 81/148 (54.73%), Query Frame = 1 
Query:   64 VSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFS-ATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFV-GALAAAAY 501
            ++++  + Y VAQ LGA     L++ F+ H       G   V+ G  +   +  EI+ TF LVYTV++ A DPK+ +    +  +AP+ IGF V    LA  P +G  +NPARSFG AV   +   +  +W++WVGP + G LA   Y
Sbjct:   96 ITVLTGLFYWVAQLLGASVACLLLR-FVTHGKAIPTHG---VSGGTTELEGVVFEIVITFALVYTVYATAADPKKGS----LGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVAAAD---FAGNWVYWVGPLIGGGLAGLVY 232
The following BLAST results are available for this feature:
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 159
Match NameE-valueIdentityDescription
AQPZ_ECOLI5.817e-1431.08Aquaporin Z OS=Escherichia coli (strain K12) GN=aq... [more]
AQPZ_ECOL65.817e-1431.08Aquaporin Z OS=Escherichia coli O6 GN=aqpZ PE=3 SV... [more]
AQPZ_ECO575.817e-1431.08Aquaporin Z OS=Escherichia coli O157:H7 GN=aqpZ PE... [more]
TIP43_ORYSJ1.076e-1335.53Probable aquaporin TIP4-3 OS=Oryza sativa subsp. j... [more]
TIP11_ORYSJ1.076e-1333.33Probable aquaporin TIP1-1 OS=Oryza sativa subsp. j... [more]
TIP43_MAIZE1.272e-1329.49Aquaporin TIP4-3 OS=Zea mays GN=TIP4-3 PE=2 SV=1[more]
AQPZ_BRAJA1.655e-1328.39Aquaporin Z OS=Bradyrhizobium japonicum GN=aqpZ PE... [more]
TIP41_MAIZE2.145e-1331.85Aquaporin TIP4-1 OS=Zea mays GN=TIP4-1 PE=2 SV=1[more]
TIP51_ARATH2.786e-1328.76Probable aquaporin TIP5-1 OS=Arabidopsis thaliana ... [more]
TIP22_MAIZE3.132e-1336.49Aquaporin TIP2-2 OS=Zea mays GN=TIP2-2 PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00218 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00218 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623127 ID=BQ623127; Name=BQ623127; organism=Citrus sinensis; type=EST; length=610bp
CTGGCATTTCTGGTGGTCACATTAACCCTGCTGTGACGTTTGGGCCTGTT
CTTGGCAAGGAAGGTATCATTGATCAGGGCTGTGGCTTACATGGTGGCTC
AGTGCTTGGGTGCCATTTGCGGTGTTGGTTTGGTCAAGGCCTTCATGAAG
CATGAGTACAACTCACTTGGTGGTGGTGCTAACACTGTGGCTTCTGGCTA
CAACAAAGGATCAGCCTTGGGTGCTGAGATTATTGGTACCTTCGTGCTTG
TTTACACAGTCTTCTCGGCCACAGACCCCAAGAGAAGCGCTCGTGACTCT
CACGTGCCCGTTTTGGCTCCACTTCCAATTGGATTTGCTGTGTTCATGGT
TCACTTGGCCACCATCCCCATCACTGGAACGGGCATCAACCCTGCTAGGA
GCTTTGGTGCTGCTGTTATCTACAACAATGACAAGGCCTGGGATGACCAT
TGGATCTTCTGGGTTGGTCCTTTCGTTGGAGCATTGGCTGCAGCGGCATA
CCACCAGTACATCTTGAGGGCTGCTGCTATCAAGGCTTTGGGATCATTCC
GCAACAACCCCTTCAAACTAAGAAAGCAAATTATGATGAATATGAACCAC
CAACCAGAAT
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