BQ623127

Overview
NameBQ623127
Unique NameBQ623127
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length610
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9730 contig Csv1_Contig9730:394..1001. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP14_ARATH (Probable aquaporin PIP1-4 OS=Arabidopsis thaliana GN=PIP1.4 PE=1 SV=1)

HSP 1 Score: 260.766 bits (665), Expect = 5.356e-73
Identity = 123/159 (77.36%), Postives = 135/159 (84.91%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKA 537
            K+SL RAV YM+ QCLGAICG G+VK F    Y +LGGGANTVA GY KGS LGAEIIGTFVLVYTVFSATD KRSARDSHVP+LAPLPIGFAVF+VHLATIPITGTGINPARS GAA+IYN D +WDDHWIFWVGPF+GA  AA YHQ ++RA   K+
Sbjct:  127 KLSLTRAVFYMIMQCLGAICGAGVVKGFQPTPYQTLGGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNKDHSWDDHWIFWVGPFIGAALAALYHQIVIRAIPFKS 285

HSP 2 Score: 35.039 bits (79), Expect = 5.356e-73
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            GISGGHINPAVTFG  L ++
Sbjct:  108 GISGGHINPAVTFGLFLARK 127
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP23_ORYSJ (Probable aquaporin PIP2-3 OS=Oryza sativa subsp. japonica GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 1.188e-72
Identity = 129/162 (79.63%), Postives = 139/162 (85.80%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKALGS 546
            KVSL+RAV Y+VAQ LGAICGVGLVK F    Y   GGGAN ++ GY+KG+ L AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS GAAVIYN  KAW DHWIFWVGP +GA  AAAYHQY+LRA+A K LGS
Sbjct:  124 KVSLVRAVLYIVAQSLGAICGVGLVKGFQSAFYVRYGGGANELSDGYSKGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYNQHKAWHDHWIFWVGPLIGAAIAAAYHQYVLRASAAK-LGS 284

HSP 2 Score: 34.6538 bits (78), Expect = 1.188e-72
Identity = 14/20 (70.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            G+SGGHINPAVTFG  L ++
Sbjct:  105 GVSGGHINPAVTFGLFLARK 124
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP24_ORYSJ (Aquaporin PIP2-4 OS=Oryza sativa subsp. japonica GN=PIP2-4 PE=2 SV=1)

HSP 1 Score: 261.922 bits (668), Expect = 1.549e-72
Identity = 127/166 (76.51%), Postives = 141/166 (84.94%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKALGSFRNN 558
            KVSL+RA+ YM AQCLGAICGV LVK F    Y+  GGGAN +A+GY+ G+ L AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS G AV+YNN+KAW D WIFWVGPF+GA  AA YHQ ILRA+A +  GSFR+N
Sbjct:  121 KVSLVRALLYMAAQCLGAICGVALVKGFQSSLYDRYGGGANELAAGYSTGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGVAVVYNNNKAWSDQWIFWVGPFIGAAIAALYHQVILRASA-RGYGSFRSN 285

HSP 2 Score: 32.3426 bits (72), Expect = 1.549e-72
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            G+SGGHINPAVT G  L ++
Sbjct:  102 GVSGGHINPAVTLGLFLARK 121
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP26_MAIZE (Aquaporin PIP2-6 OS=Zea mays GN=PIP2-6 PE=2 SV=1)

HSP 1 Score: 257.299 bits (656), Expect = 5.845e-72
Identity = 126/166 (75.90%), Postives = 139/166 (83.73%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKALGSFRNN 558
            KVSL+RA+ YM AQ LGAICGV LVK F    Y   GGGAN V++GY+ G+ L AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS GAAV+YNN KAW D WIFWVGPF+GA  AA YHQ +LRA+A +  GSFR+N
Sbjct:  123 KVSLVRALLYMAAQSLGAICGVALVKGFQSGFYARYGGGANEVSAGYSTGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVVYNNSKAWSDQWIFWVGPFIGAAIAALYHQIVLRASA-RGYGSFRSN 287

HSP 2 Score: 35.039 bits (79), Expect = 5.845e-72
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            GISGGHINPAVTFG  L ++
Sbjct:  104 GISGGHINPAVTFGLFLARK 123
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP12_ARATH (Aquaporin PIP1-2 OS=Arabidopsis thaliana GN=PIP1-2 PE=1 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 9.943e-72
Identity = 119/159 (74.84%), Postives = 135/159 (84.91%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKA 537
            K+SL RAV Y+V QCLGAICG G+VK F   +Y +LGGGANT+A GY KGS LGAEIIGTFVLVYTVFSATD KR+ARDSHVP+LAPLPIGFAVF+VHLATIPITGTGINPARS GAA+I+N D AWDDHW+FWVGPF+GA  AA YH  ++RA   K+
Sbjct:  126 KLSLTRAVYYIVMQCLGAICGAGVVKGFQPKQYQALGGGANTIAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIFNKDNAWDDHWVFWVGPFIGAALAALYHVIVIRAIPFKS 284

HSP 2 Score: 35.039 bits (79), Expect = 9.943e-72
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            GISGGHINPAVTFG  L ++
Sbjct:  107 GISGGHINPAVTFGLFLARK 126
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP11_VICFA (Aquaporin PIP1.1 OS=Vicia faba GN=PIP1.1 PE=2 SV=1)

HSP 1 Score: 255.758 bits (652), Expect = 1.691e-71
Identity = 119/159 (74.84%), Postives = 135/159 (84.91%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKA 537
            K+SL RA+ Y+V QCLGAICG G+VK F   +Y +LGGGANTVA GY KGS LGAEIIGTFVLVYTVFSATD KR+ARDSHVP+LAPLPIGFAVF+VHLATIPITGTGINPARS GAA+IYN D +WDDHW+FWVGPF+GA  AA YH  ++RA   K+
Sbjct:  126 KLSLTRALYYIVMQCLGAICGAGVVKGFQPKQYQALGGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNKDHSWDDHWVFWVGPFIGAALAALYHVVVIRAIPFKS 284

HSP 2 Score: 35.039 bits (79), Expect = 1.691e-71
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            GISGGHINPAVTFG  L ++
Sbjct:  107 GISGGHINPAVTFGLFLARK 126
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP11_ARATH (Aquaporin PIP1-1 OS=Arabidopsis thaliana GN=PIP1-1 PE=1 SV=1)

HSP 1 Score: 255.758 bits (652), Expect = 1.691e-71
Identity = 119/159 (74.84%), Postives = 135/159 (84.91%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKA 537
            K+SL RA+ Y+V QCLGAICG G+VK F   +Y +LGGGANTVA GY KGS LGAEIIGTFVLVYTVFSATD KR+ARDSHVP+LAPLPIGFAVF+VHLATIPITGTGINPARS GAA+IYN D +WDDHW+FWVGPF+GA  AA YH  ++RA   K+
Sbjct:  126 KLSLTRALYYIVMQCLGAICGAGVVKGFQPKQYQALGGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNKDHSWDDHWVFWVGPFIGAALAALYHVVVIRAIPFKS 284

HSP 2 Score: 35.039 bits (79), Expect = 1.691e-71
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            GISGGHINPAVTFG  L ++
Sbjct:  107 GISGGHINPAVTFGLFLARK 126
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP27_ORYSJ (Probable aquaporin PIP2-7 OS=Oryza sativa subsp. japonica GN=PIP2-7 PE=2 SV=2)

HSP 1 Score: 255.758 bits (652), Expect = 6.379e-71
Identity = 120/165 (72.73%), Postives = 138/165 (83.64%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKALGSFRN 555
            K+SL+R V Y+VAQCLGAI G G+VK  MK  Y++LGGGANTV+ GY+   ALGAEI+GTF+LVYTVFSATDPKR+ARDS +PVL PLPIGFAVF+VHLATIPITGTGINPARS GAAV+YN   AW DHWIFWVGP +GA  AAAYH+ +LR  A KAL SFR+
Sbjct:  121 KLSLVRTVLYVVAQCLGAIAGAGIVKGIMKRPYDALGGGANTVSDGYSAAGALGAEIVGTFILVYTVFSATDPKRTARDSFIPVLVPLPIGFAVFVVHLATIPITGTGINPARSLGAAVLYNQHAAWKDHWIFWVGPVIGAFLAAAYHKLVLRGEAAKALSSFRS 285

HSP 2 Score: 33.113 bits (74), Expect = 6.379e-71
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            G+SGGHINPAVT G   G++
Sbjct:  102 GVSGGHINPAVTLGLFFGRK 121
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP15_ARATH (Probable aquaporin PIP1-5 OS=Arabidopsis thaliana GN=PIP1-5 PE=2 SV=2)

HSP 1 Score: 253.832 bits (647), Expect = 6.380e-71
Identity = 120/159 (75.47%), Postives = 133/159 (83.65%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKA 537
            K+SL RA+ Y+V QCLGAICG G+VK F    Y + GGGAN VA GY KGS LGAEI+GTFVLVYTVFSATD KRSARDSHVP+LAPLPIGFAVF+VHLATIPITGTGINPARS GAA+IYN D AWDDHWIFWVGPF+GA  AA YHQ ++RA   K+
Sbjct:  127 KLSLTRALFYIVMQCLGAICGAGVVKGFQPGLYQTNGGGANVVAHGYTKGSGLGAEIVGTFVLVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNKDHAWDDHWIFWVGPFIGAALAALYHQIVIRAIPFKS 285

HSP 2 Score: 35.039 bits (79), Expect = 6.380e-71
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            GISGGHINPAVTFG  L ++
Sbjct:  108 GISGGHINPAVTFGLFLARK 127
[Close]
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Match: PIP12_ORYSJ (Probable aquaporin PIP1-2 OS=Oryza sativa subsp. japonica GN=PIP1-2 PE=2 SV=3)

HSP 1 Score: 252.677 bits (644), Expect = 1.415e-70
Identity = 118/159 (74.21%), Postives = 132/159 (83.02%), Query Frame = 1 
Query:   61 KVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKA 537
            K+SL RA+ YMV QCLGAICG G+VK F K  Y + GGGAN VA GY KG  LGAEI+GTF+LVYTVFSATD KR+ARDSHVP+LAPLPIGFAVF+VHLATIPITGTGINPARS GAA+IYN   AWDDHWIFWVGPF+GA  AA YHQ ++RA   K+
Sbjct:  128 KLSLTRALFYMVMQCLGAICGAGVVKGFQKGLYETTGGGANVVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNRGHAWDDHWIFWVGPFIGAALAAIYHQVVIRAIPFKS 286

HSP 2 Score: 35.039 bits (79), Expect = 1.415e-70
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    3 GISGGHINPAVTFGPVLGKE 62
            GISGGHINPAVTFG  L ++
Sbjct:  109 GISGGHINPAVTFGLFLARK 128
The following BLAST results are available for this feature:
BLAST of BQ623127 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 159
Match NameE-valueIdentityDescription
PIP14_ARATH5.356e-7377.36Probable aquaporin PIP1-4 OS=Arabidopsis thaliana ... [more]
PIP23_ORYSJ1.188e-7279.63Probable aquaporin PIP2-3 OS=Oryza sativa subsp. j... [more]
PIP24_ORYSJ1.549e-7276.51Aquaporin PIP2-4 OS=Oryza sativa subsp. japonica G... [more]
PIP26_MAIZE5.845e-7275.90Aquaporin PIP2-6 OS=Zea mays GN=PIP2-6 PE=2 SV=1[more]
PIP12_ARATH9.943e-7274.84Aquaporin PIP1-2 OS=Arabidopsis thaliana GN=PIP1-2... [more]
PIP11_VICFA1.691e-7174.84Aquaporin PIP1.1 OS=Vicia faba GN=PIP1.1 PE=2 SV=1[more]
PIP11_ARATH1.691e-7174.84Aquaporin PIP1-1 OS=Arabidopsis thaliana GN=PIP1-1... [more]
PIP27_ORYSJ6.379e-7172.73Probable aquaporin PIP2-7 OS=Oryza sativa subsp. j... [more]
PIP15_ARATH6.380e-7175.47Probable aquaporin PIP1-5 OS=Arabidopsis thaliana ... [more]
PIP12_ORYSJ1.415e-7074.21Probable aquaporin PIP1-2 OS=Oryza sativa subsp. j... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00218 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00218 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623127 ID=BQ623127; Name=BQ623127; organism=Citrus sinensis; type=EST; length=610bp
CTGGCATTTCTGGTGGTCACATTAACCCTGCTGTGACGTTTGGGCCTGTT
CTTGGCAAGGAAGGTATCATTGATCAGGGCTGTGGCTTACATGGTGGCTC
AGTGCTTGGGTGCCATTTGCGGTGTTGGTTTGGTCAAGGCCTTCATGAAG
CATGAGTACAACTCACTTGGTGGTGGTGCTAACACTGTGGCTTCTGGCTA
CAACAAAGGATCAGCCTTGGGTGCTGAGATTATTGGTACCTTCGTGCTTG
TTTACACAGTCTTCTCGGCCACAGACCCCAAGAGAAGCGCTCGTGACTCT
CACGTGCCCGTTTTGGCTCCACTTCCAATTGGATTTGCTGTGTTCATGGT
TCACTTGGCCACCATCCCCATCACTGGAACGGGCATCAACCCTGCTAGGA
GCTTTGGTGCTGCTGTTATCTACAACAATGACAAGGCCTGGGATGACCAT
TGGATCTTCTGGGTTGGTCCTTTCGTTGGAGCATTGGCTGCAGCGGCATA
CCACCAGTACATCTTGAGGGCTGCTGCTATCAAGGCTTTGGGATCATTCC
GCAACAACCCCTTCAAACTAAGAAAGCAAATTATGATGAATATGAACCAC
CAACCAGAAT
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