BQ623147

Overview
NameBQ623147
Unique NameBQ623147
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length649
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2169 contig Csv1_Contig2169:897..1543. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A2_HUMAN (Elongation factor 1-alpha 2 OS=Homo sapiens GN=EEF1A2 PE=1 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 4.941e-63
Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 502
            L EALPGDNVGFNVKNV+VKD++RG V  +SK DP +EAA FTSQVII+NHPGQI  GY+PV+DCHT+HIA KF+EL  KIDRRSGK+LE  PK LK+GDA +V+M+P KPM VE+FS YPPLGRFAVRDMRQTVAVGVIK+VEKK
Sbjct:  299 LSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKK 444

HSP 2 Score: 34.6538 bits (78), Expect = 4.941e-63
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P  +TTEVKSVEMHH ++
Sbjct:  279 TFAPVNITTEVKSVEMHHEAL 299
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A2_BOVIN (Elongation factor 1-alpha 2 OS=Bos taurus GN=EEF1A2 PE=2 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 4.941e-63
Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 502
            L EALPGDNVGFNVKNV+VKD++RG V  +SK DP +EAA FTSQVII+NHPGQI  GY+PV+DCHT+HIA KF+EL  KIDRRSGK+LE  PK LK+GDA +V+M+P KPM VE+FS YPPLGRFAVRDMRQTVAVGVIK+VEKK
Sbjct:  299 LSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKK 444

HSP 2 Score: 34.6538 bits (78), Expect = 4.941e-63
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P  +TTEVKSVEMHH ++
Sbjct:  279 TFAPVNITTEVKSVEMHHEAL 299
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_SORMA (Elongation factor 1-alpha OS=Sordaria macrospora GN=TEF PE=3 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 4.941e-63
Identity = 105/147 (71.43%), Postives = 131/147 (89.12%), Query Frame = 2 
Query:   59 KRLQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEK 499
            ++L + +PGDNVGFNVKNV+VKD++RG VA +SK+DP   AA+FT+QVI++NHPGQ+G GYAPVLDCHT+HIA KF+EL+ KIDRR+GK +E  PKF+K+GDA +VKMIP+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK+V+K
Sbjct:  296 EQLAQGVPGDNVGFNVKNVSVKDIRRGNVAGDSKNDPPVGAASFTAQVIVLNHPGQVGAGYAPVLDCHTAHIACKFAELLEKIDRRTGKAVETSPKFIKSGDAAIVKMIPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVDK 442

HSP 2 Score: 36.5798 bits (83), Expect = 4.941e-63
Identity = 15/23 (65.22%), Postives = 18/23 (78.26%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSVCR 71
            TF PS +TTEVKSVEMHH  + +
Sbjct:  278 TFAPSNVTTEVKSVEMHHEQLAQ 300
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_AJECG (Elongation factor 1-alpha OS=Ajellomyces capsulata (strain ATCC 26029 / G186AR / H82 / RMSCC 2432) GN=TEF PE=2 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 4.941e-63
Identity = 105/151 (69.54%), Postives = 129/151 (85.43%), Query Frame = 2 
Query:   59 KRLQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPT 511
            ++LQ   PGDNVGFNVKNV+VK+++RG VA +SK+DP +   +F +QVI++NHPGQ+G GYAPVLDCHT+HIA KFSEL+ KIDRR+GK +E  PKF+K+GDA +VKM+P+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIKSV K D T
Sbjct:  296 QQLQAGYPGDNVGFNVKNVSVKEVRRGNVAGDSKNDPPKGCESFNAQVIVLNHPGQVGAGYAPVLDCHTAHIACKFSELIEKIDRRTGKSVENNPKFIKSGDAAIVKMVPSKPMCVEPFTDYPPLGRFAVRDMRQTVAVGVIKSVIKSDKT 446

HSP 2 Score: 36.5798 bits (83), Expect = 4.941e-63
Identity = 15/21 (71.43%), Postives = 18/21 (85.71%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF PS +TTEVKSVEMHH+ +
Sbjct:  278 TFAPSNVTTEVKSVEMHHQQL 298
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A3_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-3 PE=3 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 4.941e-63
Identity = 110/150 (73.33%), Postives = 132/150 (88.00%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            L E LPGDNVGFNVKNV+VKD++RG V S+SK+DPA+E+A+FT+QVII+NHPGQI  GYAPVLDCHT+HIA KFSEL+ KIDRRS K +E  PKF+K+GD+ +VKM+P+KPM VE ++DYPPLGRFAVRDMRQTVAVGVIK+VEK D  G
Sbjct:  297 LTEGLPGDNVGFNVKNVSVKDIRRGNVCSDSKNDPAKESASFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFSELIEKIDRRSEK-MEDSPKFVKSGDSAIVKMVPSKPMCVEAYTDYPPLGRFAVRDMRQTVAVGVIKAVEKVDKAG 445

HSP 2 Score: 33.4982 bits (75), Expect = 4.941e-63
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    6 FGPSGLTTEVKSVEMHHRSV 65
            F P+ +TTEVKSVEMHH ++
Sbjct:  278 FAPAAVTTEVKSVEMHHETL 297
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_ARXAD (Elongation factor 1-alpha OS=Arxula adeninivorans GN=TEF PE=3 SV=1)

HSP 1 Score: 224.942 bits (572), Expect = 6.443e-63
Identity = 106/149 (71.14%), Postives = 129/149 (86.58%), Query Frame = 2 
Query:   59 KRLQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKD 505
            ++L E LPGDNVGFNVKNV+VK+++RG VA +SK+DP +   +FT+QVII NHPGQI  GY+PVLDCHT+HIA +F EL+ KIDRRSGK++E  PKF+K GDA +V+MIP+KPM VETF++YPPLGRFAVRDMRQTVAVGVIKSVEK D
Sbjct:  295 EQLPEGLPGDNVGFNVKNVSVKEIRRGNVAGDSKNDPPKGCDSFTAQVIIFNHPGQISAGYSPVLDCHTAHIACRFDELIEKIDRRSGKKVEDSPKFVKAGDAAIVRMIPSKPMCVETFTEYPPLGRFAVRDMRQTVAVGVIKSVEKSD 443

HSP 2 Score: 37.3502 bits (85), Expect = 6.443e-63
Identity = 15/21 (71.43%), Postives = 18/21 (85.71%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P+G+TTEVKSVEMHH  +
Sbjct:  277 TFAPAGVTTEVKSVEMHHEQL 297
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A2_RAT (Elongation factor 1-alpha 2 OS=Rattus norvegicus GN=Eef1a2 PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 8.400e-63
Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 502
            L EALPGDNVGFNVKNV+VKD++RG V  +SK DP +EAA FTSQVII+NHPGQI  GY+PV+DCHT+HIA KF+EL  KIDRRSGK+LE  PK LK+GDA +V+M+P KPM VE+FS YPPLGRFAVRDMRQTVAVGVIK+VEKK
Sbjct:  299 LSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKADPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKK 444

HSP 2 Score: 34.6538 bits (78), Expect = 8.400e-63
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P  +TTEVKSVEMHH ++
Sbjct:  279 TFAPVNITTEVKSVEMHHEAL 299
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A2_MOUSE (Elongation factor 1-alpha 2 OS=Mus musculus GN=Eef1a2 PE=1 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 8.400e-63
Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 502
            L EALPGDNVGFNVKNV+VKD++RG V  +SK DP +EAA FTSQVII+NHPGQI  GY+PV+DCHT+HIA KF+EL  KIDRRSGK+LE  PK LK+GDA +V+M+P KPM VE+FS YPPLGRFAVRDMRQTVAVGVIK+VEKK
Sbjct:  299 LSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKADPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKK 444

HSP 2 Score: 34.6538 bits (78), Expect = 8.400e-63
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P  +TTEVKSVEMHH ++
Sbjct:  279 TFAPVNITTEVKSVEMHHEAL 299
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A3_HUMAN (Putative elongation factor 1-alpha-like 3 OS=Homo sapiens GN=EEF1AL3 PE=5 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 8.400e-63
Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 502
            L EALPGDNVGF VKNV+VKD++RG VA +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCH +HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V M+P KPM VE+FSDYPPLGRFAVRDMRQTVAVGVIK+V+KK
Sbjct:  299 LSEALPGDNVGFKVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHMAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKK 444

HSP 2 Score: 34.2686 bits (77), Expect = 8.400e-63
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P  +TTEVKSVEMHH ++
Sbjct:  279 TFAPVNVTTEVKSVEMHHEAL 299
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_TRIRE (Elongation factor 1-alpha OS=Trichoderma reesei GN=tef1 PE=3 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 8.400e-63
Identity = 106/147 (72.11%), Postives = 129/147 (87.76%), Query Frame = 2 
Query:   59 KRLQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEK 499
            ++L E  PGDNVGFNVKNV+VK+++RG VA +SK+DP   AA+FT+QVI+MNHPGQ+G GYAPVLDCHT+HIA KF+EL+ KIDRR+GK  E  PKF+K+GD+ +VKMIP+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK+VEK
Sbjct:  296 EQLAEGQPGDNVGFNVKNVSVKEIRRGNVAGDSKNDPPMGAASFTAQVIVMNHPGQVGAGYAPVLDCHTAHIACKFAELLEKIDRRTGKATESAPKFIKSGDSAIVKMIPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVEK 442

HSP 2 Score: 36.1946 bits (82), Expect = 8.400e-63
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF PS +TTEVKSVEMHH  +
Sbjct:  278 TFAPSNVTTEVKSVEMHHEQL 298
The following BLAST results are available for this feature:
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 171
Match NameE-valueIdentityDescription
EF1A2_HUMAN4.941e-6375.34Elongation factor 1-alpha 2 OS=Homo sapiens GN=EEF... [more]
EF1A2_BOVIN4.941e-6375.34Elongation factor 1-alpha 2 OS=Bos taurus GN=EEF1A... [more]
EF1A_SORMA4.941e-6371.43Elongation factor 1-alpha OS=Sordaria macrospora G... [more]
EF1A_AJECG4.941e-6369.54Elongation factor 1-alpha OS=Ajellomyces capsulata... [more]
EF1A3_RHIRA4.941e-6373.33Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A_ARXAD6.443e-6371.14Elongation factor 1-alpha OS=Arxula adeninivorans ... [more]
EF1A2_RAT8.400e-6375.34Elongation factor 1-alpha 2 OS=Rattus norvegicus G... [more]
EF1A2_MOUSE8.400e-6375.34Elongation factor 1-alpha 2 OS=Mus musculus GN=Eef... [more]
EF1A3_HUMAN8.400e-6376.03Putative elongation factor 1-alpha-like 3 OS=Homo ... [more]
EF1A_TRIRE8.400e-6372.11Elongation factor 1-alpha OS=Trichoderma reesei GN... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00238 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00238 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623147 ID=BQ623147; Name=BQ623147; organism=Citrus sinensis; type=EST; length=649bp
TGACATTTGGTCCCTCTGGATTGACAACTGAGGTTAAGTCTGTTGAGATG
CACCATCGAAGCGTCTGCAGGAGGCCCTTCCGGGTGACAATGTTGGCTTC
AACGTGAAGAACGTTGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCTTC
CAACTCGAAGGATGATCCGGCCAGGGAAGCTGCCAACTTCACTTCACAGG
TCATCATCATGAACCATCCTGGCCAGATTGGAAATGGATATGCCCCAGTG
CTTGACTGCCACACCTCCCACATTGCCGTCAAGTTTTCTGAGCTCGTGAC
GAAGATTGATAGGCGATCAGGCAAGGAGCTTGAGAAGGAGCCCAAGTTCT
TGAAGAATGGAGATGCCGGAATGGTTAAGATGATTCCCACCAAGCCCATG
GTTGTTGAGACATTCTCCGACTACCCACCCCTTGGTCGTTTTGCTGTTAG
GGACATGCGTCAGACTGTTGCTGTGGGTGTTATCAAGAGTGTTGAGAAGA
AGGATCCCACAGGTGCCAAGGTCACCAAGTCCGCAGCCAAGAAGAAATGA
GAAATTTTTATGGGTCTGTCTACAATAGTTAGGGGTTATTTTCTGTCTTT
TTTATTTTCTTTTCTGGTTTTGGTTTGCATCAATGTTTCTGCAAACAGA
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