BQ623147

Overview
NameBQ623147
Unique NameBQ623147
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length649
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2169 contig Csv1_Contig2169:897..1543. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_HELGL (Elongation factor 1-alpha (Fragment) OS=Helicoverpa gelotopoeon PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 7.370e-52
Identity = 84/129 (65.12%), Postives = 112/129 (86.82%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVR 451
            LQEA+PGDNVGFNVKNV+VK+L+RG+VA +SK++P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRR+GK  E  PK +K+GDA +V ++P+KP+ VE+F ++PPLGRFAVR
Sbjct:  285 LQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 413

HSP 2 Score: 33.8834 bits (76), Expect = 7.370e-52
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    6 FGPSGLTTEVKSVEMHHRSV 65
            F P+ +TTEVKSVEMHH ++
Sbjct:  266 FAPANITTEVKSVEMHHEAL 285
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_HELDI (Elongation factor 1-alpha (Fragment) OS=Heliocheilus discalis PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 7.370e-52
Identity = 84/129 (65.12%), Postives = 112/129 (86.82%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVR 451
            LQEA+PGDNVGFNVKNV+VK+L+RG+VA +SK++P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRR+GK  E  PK +K+GDA +V ++P+KP+ VE+F ++PPLGRFAVR
Sbjct:  285 LQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 413

HSP 2 Score: 33.8834 bits (76), Expect = 7.370e-52
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    6 FGPSGLTTEVKSVEMHHRSV 65
            F P+ +TTEVKSVEMHH ++
Sbjct:  266 FAPANITTEVKSVEMHHEAL 285
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_HELAM (Elongation factor 1-alpha (Fragment) OS=Helicoverpa armigera PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 7.370e-52
Identity = 84/129 (65.12%), Postives = 112/129 (86.82%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVR 451
            LQEA+PGDNVGFNVKNV+VK+L+RG+VA +SK++P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRR+GK  E  PK +K+GDA +V ++P+KP+ VE+F ++PPLGRFAVR
Sbjct:  285 LQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 413

HSP 2 Score: 33.8834 bits (76), Expect = 7.370e-52
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    6 FGPSGLTTEVKSVEMHHRSV 65
            F P+ +TTEVKSVEMHH ++
Sbjct:  266 FAPANITTEVKSVEMHHEAL 285
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_HELAL (Elongation factor 1-alpha (Fragment) OS=Heliocheilus albipunctella PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 7.370e-52
Identity = 84/129 (65.12%), Postives = 112/129 (86.82%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVR 451
            LQEA+PGDNVGFNVKNV+VK+L+RG+VA +SK++P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRR+GK  E  PK +K+GDA +V ++P+KP+ VE+F ++PPLGRFAVR
Sbjct:  285 LQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 413

HSP 2 Score: 33.8834 bits (76), Expect = 7.370e-52
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    6 FGPSGLTTEVKSVEMHHRSV 65
            F P+ +TTEVKSVEMHH ++
Sbjct:  266 FAPANITTEVKSVEMHHEAL 285
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_ANIIF (Elongation factor 1-alpha (Fragment) OS=Anicla infecta PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 7.370e-52
Identity = 84/129 (65.12%), Postives = 112/129 (86.82%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVR 451
            LQEA+PGDNVGFNVKNV+VK+L+RG+VA +SK++P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRR+GK  E  PK +K+GDA +V ++P+KP+ VE+F ++PPLGRFAVR
Sbjct:  285 LQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 413

HSP 2 Score: 33.8834 bits (76), Expect = 7.370e-52
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    6 FGPSGLTTEVKSVEMHHRSV 65
            F P+ +TTEVKSVEMHH ++
Sbjct:  266 FAPANITTEVKSVEMHHEAL 285
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_ADIBE (Elongation factor 1-alpha (Fragment) OS=Adisura bella PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 7.370e-52
Identity = 84/129 (65.12%), Postives = 112/129 (86.82%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVR 451
            LQEA+PGDNVGFNVKNV+VK+L+RG+VA +SK++P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRR+GK  E  PK +K+GDA +V ++P+KP+ VE+F ++PPLGRFAVR
Sbjct:  285 LQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 413

HSP 2 Score: 33.8834 bits (76), Expect = 7.370e-52
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:    6 FGPSGLTTEVKSVEMHHRSV 65
            F P+ +TTEVKSVEMHH ++
Sbjct:  266 FAPANITTEVKSVEMHHEAL 285
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_BLAHO (Elongation factor 1-alpha OS=Blastocystis hominis PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 4.262e-49
Identity = 84/142 (59.15%), Postives = 114/142 (80.28%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKS 490
            + +ALPGDNVGFNV NV+V+D+ RG V  ++K+DP  +  +  +QVI+MNHP  I  GY PV+DCHT+HIA KF ++++++D+R+GK L + P  +KNG + M +++P+KPM VETFSDYPPLGRFAVRDMRQTVAVG+IKS
Sbjct:  289 IPQALPGDNVGFNVNNVSVEDIHRGNVCGDAKNDPPCKTES-DAQVIVMNHPSGIRPGYCPVVDCHTAHIACKFEKIMSEMDKRTGKVLRENPDIVKNGKSMMAQLVPSKPMCVETFSDYPPLGRFAVRDMRQTVAVGIIKS 429

HSP 2 Score: 34.6538 bits (78), Expect = 4.262e-49
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P  ++TEVKSVEMHH S+
Sbjct:  269 TFAPVNVSTEVKSVEMHHESI 289
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A2_EUPCR (Elongation factor 1-alpha 2 OS=Euplotes crassus GN=EFA2 PE=3 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 6.400e-44
Identity = 83/155 (53.55%), Postives = 116/155 (74.84%), Query Frame = 2 
Query:   47 DAPSKRLQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPT 511
            +A   +L+EA PG NVGFNV+ +  K++K G V  ++K+DP + A +F +QVI+MNHPG I  GY PVLD HT+H+A KF  L++K + R+GK +E+ PKFLKNG++G+V+++PTKP+ VE FS Y  LGRF +RDM++TVAVGVI+ V  K  T
Sbjct:  279 EAHHNKLEEAEPGLNVGFNVR-LEAKEIKAGHVCGDAKNDPPKNAESFIAQVIVMNHPGHIKAGYQPVLDIHTAHVATKFKTLLSKNEARTGKLIEEAPKFLKNGESGIVELVPTKPLCVEEFSKYAALGRFVIRDMKRTVAVGVIQEVIHKKET 432

HSP 2 Score: 24.6386 bits (52), Expect = 6.400e-44
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 3 
Query:   15 SGLTTEVKSVEMHHRSV 65
            +G T+EVKS+E HH  +
Sbjct:  269 TGATSEVKSLEAHHNKL 285
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_ARCFU (Elongation factor 1-alpha OS=Archaeoglobus fulgidus GN=tuf PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 5.608e-39
Identity = 70/140 (50.00%), Postives = 100/140 (71.43%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVI 484
            +QEA PGDN+GFNV+ V+ KD++RG VA +  D+P     +FT+Q++++ HP  I  GY PV+  HT+ IA +F EL  KID R+G+  E+ P+FLK GDA +VK+ PT+PMV+E   D PP+GRFA+RDM  T+  G++
Sbjct:  278 IQEAYPGDNIGFNVRGVSKKDIRRGDVAGHP-DNPPTVVKDFTAQLVVLQHPTAITVGYTPVVHAHTAQIACRFVELQKKIDPRTGQVKEENPQFLKTGDAAIVKLEPTRPMVIERVKDIPPMGRFAIRDMGMTIGAGMV 416

HSP 2 Score: 30.4166 bits (67), Expect = 5.608e-39
Identity = 11/20 (55.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:    6 FGPSGLTTEVKSVEMHHRSV 65
            F P+G++ EVKS+EMHH  +
Sbjct:  259 FEPAGVSGEVKSIEMHHEPI 278
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_METJA (Elongation factor 1-alpha OS=Methanocaldococcus jannaschii GN=tuf PE=3 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 2.104e-38
Identity = 70/149 (46.98%), Postives = 103/149 (69.13%), Query Frame = 2 
Query:   59 KRLQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKD 505
            +++ +A PGDN+GFNV+ V+ KD+KRG V  +  D+P   A  FT+Q++++ HP  I  GY PV   HT+ +A  F EL+ K+D R+G+ +E+ P+FLK GDA +VK+ PTKPMV+E   + P LGRFA+RDM  T+A G+   V+ K+
Sbjct:  280 EQIPQAEPGDNIGFNVRGVSKKDIKRGDVCGHP-DNPPTVAEEFTAQIVVLQHPTAITVGYTPVFHAHTAQVACTFIELLKKLDPRTGQVIEENPQFLKTGDAAIVKIKPTKPMVIENVREIPQLGRFAIRDMGMTIAAGMAIDVKAKN 427

HSP 2 Score: 30.8018 bits (68), Expect = 2.104e-38
Identity = 11/20 (55.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:    6 FGPSGLTTEVKSVEMHHRSV 65
            F P+G++ EVKS+EMHH  +
Sbjct:  263 FEPAGVSGEVKSIEMHHEQI 282
The following BLAST results are available for this feature:
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 171
Match NameE-valueIdentityDescription
EF1A_HELGL7.370e-5265.12Elongation factor 1-alpha (Fragment) OS=Helicoverp... [more]
EF1A_HELDI7.370e-5265.12Elongation factor 1-alpha (Fragment) OS=Heliocheil... [more]
EF1A_HELAM7.370e-5265.12Elongation factor 1-alpha (Fragment) OS=Helicoverp... [more]
EF1A_HELAL7.370e-5265.12Elongation factor 1-alpha (Fragment) OS=Heliocheil... [more]
EF1A_ANIIF7.370e-5265.12Elongation factor 1-alpha (Fragment) OS=Anicla inf... [more]
EF1A_ADIBE7.370e-5265.12Elongation factor 1-alpha (Fragment) OS=Adisura be... [more]
EF1A_BLAHO4.262e-4959.15Elongation factor 1-alpha OS=Blastocystis hominis ... [more]
EF1A2_EUPCR6.400e-4453.55Elongation factor 1-alpha 2 OS=Euplotes crassus GN... [more]
EF1A_ARCFU5.608e-3950.00Elongation factor 1-alpha OS=Archaeoglobus fulgidu... [more]
EF1A_METJA2.104e-3846.98Elongation factor 1-alpha OS=Methanocaldococcus ja... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00238 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00238 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623147 ID=BQ623147; Name=BQ623147; organism=Citrus sinensis; type=EST; length=649bp
TGACATTTGGTCCCTCTGGATTGACAACTGAGGTTAAGTCTGTTGAGATG
CACCATCGAAGCGTCTGCAGGAGGCCCTTCCGGGTGACAATGTTGGCTTC
AACGTGAAGAACGTTGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCTTC
CAACTCGAAGGATGATCCGGCCAGGGAAGCTGCCAACTTCACTTCACAGG
TCATCATCATGAACCATCCTGGCCAGATTGGAAATGGATATGCCCCAGTG
CTTGACTGCCACACCTCCCACATTGCCGTCAAGTTTTCTGAGCTCGTGAC
GAAGATTGATAGGCGATCAGGCAAGGAGCTTGAGAAGGAGCCCAAGTTCT
TGAAGAATGGAGATGCCGGAATGGTTAAGATGATTCCCACCAAGCCCATG
GTTGTTGAGACATTCTCCGACTACCCACCCCTTGGTCGTTTTGCTGTTAG
GGACATGCGTCAGACTGTTGCTGTGGGTGTTATCAAGAGTGTTGAGAAGA
AGGATCCCACAGGTGCCAAGGTCACCAAGTCCGCAGCCAAGAAGAAATGA
GAAATTTTTATGGGTCTGTCTACAATAGTTAGGGGTTATTTTCTGTCTTT
TTTATTTTCTTTTCTGGTTTTGGTTTGCATCAATGTTTCTGCAAACAGA
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