BQ623282

Overview
NameBQ623282
Unique NameBQ623282
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length626
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:361..985. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_XYLFA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa GN=metE PE=3 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.225e-44
Identity = 91/153 (59.48%), Postives = 110/153 (71.90%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQ 460
            A+REGLPLR+ E A YL+WAV SFRI +  V+DTTQIHTHMCYS FNDII ++  +DADVI+IE SRS  +LL  F +  +Y   IGPGVYDIHSPR+P  EE+   + K  AVL    LWVNPDCGLKTR + E + AL  MV AA+ LR +
Sbjct:  607 AIREGLPLRRGEWADYLNWAVESFRIASSNVRDTTQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVK-FRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLPPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE 758
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_RHOPT (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhodopseudomonas palustris (strain TIE-1) GN=metE PE=3 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.225e-44
Identity = 93/151 (61.59%), Postives = 108/151 (71.52%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 454
            ALREGLPLR+SE   YLDWAV  FR+ + GV+D TQIHTHMCYS FNDII +I  MDADVI+IE SRS  +LL  F+   KY   IGPGVYDIHSPR+PS EE+   +      L    LWVNPDCGLKTR + EV+ AL NMV AA+ +R
Sbjct:  634 ALREGLPLRRSEWKGYLDWAVECFRLCSSGVKDETQIHTHMCYSEFNDIIDAIAAMDADVISIETSRSKMELLDAFKT-YKYPNEIGPGVYDIHSPRVPSVEEMTTLLQLARQRLSDGQLWVNPDCGLKTRGWDEVQGALVNMVEAARQIR 783
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_RHOPA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhodopseudomonas palustris GN=metE PE=3 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.225e-44
Identity = 93/151 (61.59%), Postives = 108/151 (71.52%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 454
            ALREGLPLR+SE   YLDWAV  FR+ + GV+D TQIHTHMCYS FNDII +I  MDADVI+IE SRS  +LL  F+   KY   IGPGVYDIHSPR+PS EE+   +      L    LWVNPDCGLKTR + EV+ AL NMV AA+ +R
Sbjct:  634 ALREGLPLRRSEWKGYLDWAVECFRLCSSGVKDETQIHTHMCYSEFNDIIDAIAAMDADVISIETSRSKMELLDAFKT-YKYPNEIGPGVYDIHSPRVPSVEEMTTLLQLARQRLSDGQLWVNPDCGLKTRGWDEVQGALVNMVEAARQIR 783
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_PSEF5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=metE PE=3 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.225e-44
Identity = 90/155 (58.06%), Postives = 108/155 (69.68%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 466
            A REGLPLR+++   YLDWA   FR+   GV+D TQIHTHMCYS FND+I SI  MDADVITIE SRSD +LL  F+    Y   IGPGVYDIHSPR+P   E+A+ + K    +    LWVNPDCGLKTR + E + AL +MV AA+ LR +LA
Sbjct:  609 AFREGLPLRRAQWQHYLDWATEVFRLCASGVRDETQIHTHMCYSEFNDVIESIAAMDADVITIETSRSDMELLDAFK-AFAYPNEIGPGVYDIHSPRVPDASEMANLLRKAAQRIPAERLWVNPDCGLKTRGWPETEAALVHMVTAARQLRKELA 762
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_XYLFT (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=metE PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.600e-44
Identity = 91/153 (59.48%), Postives = 109/153 (71.24%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQ 460
            A+REGLPLR+ E A YL+WAV SFRI +  V DTTQIHTHMCYS FNDII ++  +DADVI+IE SRS  +LL  F +  +Y   IGPGVYDIHSPR+P  EE+   + K  AVL    LWVNPDCGLKTR + E + AL  MV AA+ LR +
Sbjct:  607 AIREGLPLRRGEWADYLNWAVESFRIASSNVHDTTQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVK-FRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLPPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE 758
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_XYLFM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain M12) GN=metE PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.600e-44
Identity = 91/153 (59.48%), Postives = 109/153 (71.24%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQ 460
            A+REGLPLR+ E A YL+WAV SFRI +  V DTTQIHTHMCYS FNDII ++  +DADVI+IE SRS  +LL  F +  +Y   IGPGVYDIHSPR+P  EE+   + K  AVL    LWVNPDCGLKTR + E + AL  MV AA+ LR +
Sbjct:  607 AIREGLPLRRGEWADYLNWAVESFRIASSNVHDTTQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVK-FRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLPPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE 758
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_XYLF2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain M23) GN=metE PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.600e-44
Identity = 91/153 (59.48%), Postives = 109/153 (71.24%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQ 460
            A+REGLPLR+ E A YL+WAV SFRI +  V DTTQIHTHMCYS FNDII ++  +DADVI+IE SRS  +LL  F +  +Y   IGPGVYDIHSPR+P  EE+   + K  AVL    LWVNPDCGLKTR + E + AL  MV AA+ LR +
Sbjct:  607 AIREGLPLRRGEWADYLNWAVESFRIASSNVHDTTQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVK-FRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLPPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE 758
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_COXB1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Coxiella burnetii (strain CbuK_Q154) GN=metE PE=3 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 3.565e-44
Identity = 91/158 (57.59%), Postives = 109/158 (68.99%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 475
            A RE LPLRK+    YL+WAV  FR+ +CGV+D TQIHTHMCYS FNDII +I  +DADVITIE+SRS+ ++L  F E   Y   IGPG+YDIHSPRIP   EI +   + L  +    LW+NPDCGLKTR + E K ALS MV AAK LR   +S K
Sbjct:  606 AFRECLPLRKAAWQDYLEWAVKCFRLASCGVKDETQIHTHMCYSEFNDIIEAIAALDADVITIESSRSETEILKSF-EKFAYPNDIGPGIYDIHSPRIPRVAEIEELAVRALQYIPIERLWINPDCGLKTRNWEETKEALSRMVDAAKHLRKAFSSEK 762
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_RHILO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhizobium loti GN=metE PE=3 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 4.656e-44
Identity = 91/157 (57.96%), Postives = 110/157 (70.06%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASA 472
            ALREGLPLR+++   YLDWAV  FR+ +  V D TQIHTHMCYS F DI+ +I  MDADVI+IE SRS  +LL   R   KY   IGPGVYDIHSPR+P   EI++ I      L    LWVNPDCGLKTR++ EV+PAL NMVAAA+ +R +  +A
Sbjct:  621 ALREGLPLREADWQAYLDWAVECFRLASTAVGDATQIHTHMCYSEFGDIVDAIAAMDADVISIETSRSQLELLDTLRT-FKYPNEIGPGVYDIHSPRVPEVGEISNLIMLARKRLSDGQLWVNPDCGLKTRRWEEVRPALVNMVAAARAIRQKAQTA 776
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_COXBU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Coxiella burnetii GN=metE PE=3 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 4.656e-44
Identity = 91/158 (57.59%), Postives = 109/158 (68.99%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 475
            A RE LPLRK+    YL+WAV  FR+ +CGV+D TQIHTHMCYS FNDII +I  +DADVITIE+SRS+ ++L  F E   Y   IGPG+YDIHSPRIP   EI +   + L  +    LW+NPDCGLKTR + E K ALS MV AAK LR   +S K
Sbjct:  606 AFRECLPLRKAAWQDYLEWAVKCFRLASCGVKDETQIHTHMCYSEFNDIIEAIAALDADVITIESSRSEMEILKSF-EKFAYPNDIGPGIYDIHSPRIPRVAEIEELAVRALQYIPIERLWINPDCGLKTRNWEETKEALSRMVDAAKHLRKAFSSEK 762
The following BLAST results are available for this feature:
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 274
Match NameE-valueIdentityDescription
METE_XYLFA1.225e-4459.485-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHOPT1.225e-4461.595-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHOPA1.225e-4461.595-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PSEF51.225e-4458.065-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFT1.600e-4459.485-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFM1.600e-4459.485-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLF21.600e-4459.485-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_COXB13.565e-4457.595-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHILO4.656e-4457.965-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_COXBU4.656e-4457.595-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00373 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00373 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623282 ID=BQ623282; Name=BQ623282; organism=Citrus sinensis; type=EST; length=626bp
TGCCTTGAGGGAAGGATTGCCTCTTAGGAAATCTGAGCATGCTTTCTACT
TGGACTGGGCAGTCCACTCCTTCAGAATCACCAACTGTGGCGTCCAGGAC
ACTACCCAGATCCACACCCACATGTGCTACTCCAACTTCAACGACATTAT
CCACTCAATTATCGACATGGATGCTGATGTGATCACCATTGAGAACTCTC
GTTCTGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGTCAAGTATGGT
GCTGGAATTGGCCCTGGTGTGTATGACATCCACTCTCCAAGAATACCATC
AACTGAAGAAATTGCTGACAGAATCAACAAGATGCTTGCTGTGCTAGAAA
CCAACATTTTGTGGGTTAACCCTGACTGTGGTCTTAAGACTCGCAAGTAC
ACTGAGGTGAAGCCAGCCCTCTCAAACATGGTCGCTGCTGCCAAGCTACT
CCGCACCCAGCTTGCCAGTGCCAAGTGAGTGGTTCTGGAGATTGGAGGGA
TACTTCCTGAGCCATTGATTTTTTTTTTTTTCACTTTAAACAAAGAAAGA
AAATTCCTGTCTTATTACCTATTCAAATACTGGGGGTCCGGAAGGGATGA
AATTTTCCCTATGCATATATGCACTA
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