BQ623325 | Citrus Genome Database

Overview


Name	BQ623325
Unique Name	BQ623325
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	512

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig4610	contig	Csv1_Contig4610:526..1036.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP11_VICFA (Aquaporin PIP1.1 OS=Vicia faba GN=PIP1.1 PE=2 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 2.874e-56
Identity = 96/123 (78.05%), Postives = 111/123 (90.24%), Query Frame = 2

Query:   65 GGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKA 433
            GGGAN +A GY+ G+GLGAEIIGTFVLVYTVFSATD KRNARDSHVP+LAPLPIGFAVF+VHLATIP+TGTGINPARSLGAA+IY+K+ +WDD W+FWVGPFIGAA+AA YH  ++RA   K+
Sbjct:  162 GGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNKDHSWDDHWVFWVGPFIGAALAALYHVVVIRAIPFKS 284

HSP 2 Score: 29.6462 bits (65), Expect = 2.874e-56
Identity = 12/18 (66.67%), Postives = 13/18 (72.22%), Query Frame = 1

Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAICG G+VK FQ   Y
Sbjct:  141 LGAICGAGVVKGFQPKQY 158

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP11_ARATH (Aquaporin PIP1-1 OS=Arabidopsis thaliana GN=PIP1-1 PE=1 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 2.874e-56
Identity = 96/123 (78.05%), Postives = 111/123 (90.24%), Query Frame = 2

Query:   65 GGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKA 433
            GGGAN +A GY+ G+GLGAEIIGTFVLVYTVFSATD KRNARDSHVP+LAPLPIGFAVF+VHLATIP+TGTGINPARSLGAA+IY+K+ +WDD W+FWVGPFIGAA+AA YH  ++RA   K+
Sbjct:  162 GGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNKDHSWDDHWVFWVGPFIGAALAALYHVVVIRAIPFKS 284

HSP 2 Score: 29.6462 bits (65), Expect = 2.874e-56
Identity = 12/18 (66.67%), Postives = 13/18 (72.22%), Query Frame = 1

Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAICG G+VK FQ   Y
Sbjct:  141 LGAICGAGVVKGFQPKQY 158

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP13_ORYSJ (Aquaporin PIP 1-3 OS=Oryza sativa subsp. japonica GN=PIP1-3 PE=2 SV=2)

HSP 1 Score: 201.83 bits (512), Expect = 1.535e-54
Identity = 93/123 (75.61%), Postives = 107/123 (86.99%), Query Frame = 2

Query:   65 GGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKA 433
            GGGAN +  GY+ G GLGAEI+GTFVLVYTVFSATD KRNARDSHVP+LAPLPIGFAVF+VHLATIP+TGTGINPARSLGAA++Y++  AW D WIFWVGPFIGAA+AA YH  ++RA   K+
Sbjct:  164 GGGANAVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIVYNRAHAWHDHWIFWVGPFIGAALAAIYHVVVIRAIPFKS 286

HSP 2 Score: 31.187 bits (69), Expect = 1.535e-54
Identity = 12/19 (63.16%), Postives = 15/19 (78.95%), Query Frame = 1

Query:    1 LGAICGCGLVKAFQKSYYI 57
            LGAICG G+VK FQ+  Y+
Sbjct:  143 LGAICGAGVVKGFQRGLYM 161

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP15_MAIZE (Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 2.608e-54
Identity = 93/123 (75.61%), Postives = 108/123 (87.80%), Query Frame = 2

Query:   65 GGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKA 433
            GGGAN +  GY+ G GLGAEI+GTFVLVYTVFSATD KR+ARDSHVP+LAPLPIGFAVF+VHLATIP+TGTGINPARSLGAA++Y++  AW+D WIFWVGPFIGAA+AA YH  I+RA   K+
Sbjct:  164 GGGANAVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIVYNRSHAWNDHWIFWVGPFIGAALAAIYHVVIIRALPFKS 286

HSP 2 Score: 30.8018 bits (68), Expect = 2.608e-54
Identity = 12/19 (63.16%), Postives = 15/19 (78.95%), Query Frame = 1

Query:    1 LGAICGCGLVKAFQKSYYI 57
            LGAICG G+VK FQ+  Y+
Sbjct:  143 LGAICGAGVVKGFQEGLYM 161

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP1_SOLLC (Probable aquaporin PIP-type pTOM75 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 4.432e-54
Identity = 96/118 (81.36%), Postives = 106/118 (89.83%), Query Frame = 2

Query:   65 GGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRA 418
            GGGAN +  GY+ G GLGAEIIGTFVLVYTVFSATD KRNARDSHVP+LAPLPIGFAVF+VHLATIP+TGTGINPARSLGAA+IY+ E AW+D WIFWVGP IGAA+AA YHQ I+RA
Sbjct:  163 GGGANVVNPGYTKGDGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNDEHAWNDHWIFWVGPMIGAALAAIYHQIIIRA 280

HSP 2 Score: 26.5646 bits (57), Expect = 4.432e-54
Identity = 11/18 (61.11%), Postives = 12/18 (66.67%), Query Frame = 1

Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAICG G+VK F    Y
Sbjct:  142 LGAICGAGVVKGFMVGPY 159

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP27_ORYSJ (Probable aquaporin PIP2-7 OS=Oryza sativa subsp. japonica GN=PIP2-7 PE=2 SV=2)

HSP 1 Score: 204.912 bits (520), Expect = 3.696e-53
Identity = 95/129 (73.64%), Postives = 110/129 (85.27%), Query Frame = 2

Query:   65 GGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            GGGAN ++DGYS    LGAEI+GTF+LVYTVFSATDPKR ARDS +PVL PLPIGFAVF+VHLATIP+TGTGINPARSLGAAV+Y++  AW D WIFWVGP IGA +AA YH+ +LR  A+KAL SF+S
Sbjct:  157 GGGANTVSDGYSAAGALGAEIVGTFILVYTVFSATDPKRTARDSFIPVLVPLPIGFAVFVVHLATIPITGTGINPARSLGAAVLYNQHAAWKDHWIFWVGPVIGAFLAAAYHKLVLRGEAAKALSSFRS 285

HSP 2 Score: 23.483 bits (49), Expect = 3.696e-53
Identity = 10/18 (55.56%), Postives = 11/18 (61.11%), Query Frame = 1

Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAI G G+VK   K  Y
Sbjct:  136 LGAIAGAGIVKGIMKRPY 153

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP2_PEA (Probable aquaporin PIP-type 7a OS=Pisum sativum GN=TRG-31 PE=2 SV=2)

HSP 1 Score: 200.29 bits (508), Expect = 1.067e-52
Identity = 91/122 (74.59%), Postives = 109/122 (89.34%), Query Frame = 2

Query:   68 GGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKA 433
            GGAN +A GY+ G GLGAEI+GTF+LVYTVFSATD KR+ARDSHVP+LAPLPIGFAVF+VHLATIP+TGTGINPARSLGAA++++K+  W+D WIFWVGPFIGAA+AA YHQ ++RA   K+
Sbjct:  167 GGANFVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIVFNKKIGWNDHWIFWVGPFIGAALAALYHQVVIRAIPFKS 288

HSP 2 Score: 26.5646 bits (57), Expect = 1.067e-52
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 1

Query:    1 LGAICGCGLVKAFQ 42
            LGAICG G+VK F+
Sbjct:  144 LGAICGAGVVKGFE 157

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP11_MAIZE (Aquaporin PIP1-1 OS=Zea mays GN=PIP1-1 PE=1 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 5.237e-52
Identity = 92/123 (74.80%), Postives = 105/123 (85.37%), Query Frame = 2

Query:   65 GGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKA 433
            GG  N +A GY+ G GLGAEI+GTF+LVYTVFSATD KR ARDSHVP+LAPLPIGFAVF+VHLAT+ +TGTGINPARSLGAAVIY++  AW D WIFWVGPFIGAA+AA YHQ I+RA   K+
Sbjct:  163 GGRRNVVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRRARDSHVPILAPLPIGFAVFLVHLATMGITGTGINPARSLGAAVIYNQHHAWADHWIFWVGPFIGAALAAIYHQVIIRAIPFKS 285

HSP 2 Score: 29.6462 bits (65), Expect = 5.237e-52
Identity = 12/19 (63.16%), Postives = 15/19 (78.95%), Query Frame = 1

Query:    1 LGAICGCGLVKAFQKSYYI 57
            LGAICG G+VK FQ+  Y+
Sbjct:  142 LGAICGRGVVKGFQQGLYM 160

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP16_MAIZE (Aquaporin PIP1-6 OS=Zea mays GN=PIP1-6 PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 6.825e-52
Identity = 91/123 (73.98%), Postives = 102/123 (82.93%), Query Frame = 2

Query:   65 GGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKA 433
            GGGAN ++ GY+ G GLGAE++GTFVLVYTVFSATD KR ARDSHVP LAPLPIGFAVF+VHLATIP+TGTGINPARSLGAA+IY     W   WIFWVGPF GAA+AA YHQ +LRA   K+
Sbjct:  170 GGGANAVSPGYTKGDGLGAEVVGTFVLVYTVFSATDAKRTARDSHVPALAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYDNPHGWHGHWIFWVGPFAGAALAAVYHQVVLRAIPFKS 292

HSP 2 Score: 28.8758 bits (63), Expect = 6.825e-52
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 1

Query:    1 LGAICGCGLVKAFQKSYY 54
            LGA+CG G+VKAF  + Y
Sbjct:  149 LGAVCGAGVVKAFGSALY 166

[Close]

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP28_ORYSJ (Probable aquaporin PIP2-8 OS=Oryza sativa subsp. japonica GN=PIP2-8 PE=2 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 1.487e-48
Identity = 93/129 (72.09%), Postives = 104/129 (80.62%), Query Frame = 2

Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVI--YSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGS 442
            +GGGANELA GYS G G+ AE++GTFVLVYTVFSATDPKR ARDSHVPVLAPLPIG AV +VHLATIP+TGTGINPARSLG A++      KAW   WIFWVGPF GAA A  YH +ILR +A+KA  S
Sbjct:  144 HGGGANELAAGYSAGAGVVAEMVGTFVLVYTVFSATDPKRKARDSHVPVLAPLPIGLAVLVVHLATIPITGTGINPARSLGPALVLGLGTTKAWSHLWIFWVGPFAGAAAAMIYHHYILRGAAAKAFAS 272

HSP 2 Score: 21.9422 bits (45), Expect = 1.487e-48
Identity = 7/12 (58.33%), Postives = 10/12 (83.33%), Query Frame = 1

Query:    1 LGAICGCGLVKA 36
            +GA+CG GL +A
Sbjct:  123 VGAVCGAGLARA 134

The following BLAST results are available for this feature:

BLAST of BQ623325 vs. ExPASy Swiss-Prot

BLAST of BQ623325 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 132


Match Name	E-value	Identity	Description
PIP11_VICFA	2.874e-56	78.05	Aquaporin PIP1.1 OS=Vicia faba GN=PIP1.1 PE=2 SV=1	[more]
PIP11_ARATH	2.874e-56	78.05	Aquaporin PIP1-1 OS=Arabidopsis thaliana GN=PIP1-1...	[more]
PIP13_ORYSJ	1.535e-54	75.61	Aquaporin PIP 1-3 OS=Oryza sativa subsp. japonica ...	[more]
PIP15_MAIZE	2.608e-54	75.61	Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1	[more]
PIP1_SOLLC	4.432e-54	81.36	Probable aquaporin PIP-type pTOM75 OS=Solanum lyco...	[more]
PIP27_ORYSJ	3.696e-53	73.64	Probable aquaporin PIP2-7 OS=Oryza sativa subsp. j...	[more]
PIP2_PEA	1.067e-52	74.59	Probable aquaporin PIP-type 7a OS=Pisum sativum GN...	[more]
PIP11_MAIZE	5.237e-52	74.80	Aquaporin PIP1-1 OS=Zea mays GN=PIP1-1 PE=1 SV=1	[more]
PIP16_MAIZE	6.825e-52	73.98	Aquaporin PIP1-6 OS=Zea mays GN=PIP1-6 PE=2 SV=1	[more]
PIP28_ORYSJ	1.487e-48	72.09	Probable aquaporin PIP2-8 OS=Oryza sativa subsp. j...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_00416 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00416 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623325 ID=BQ623325; Name=BQ623325; organism=Citrus sinensis; type=EST; length=512bp
TTGGGAGCCATATGTGGCTGCGGGCTCGTTAAGGCTTTCCAGAAGTCTTA
CTACATCGAGATACGGCGGTGGAGCCAATGAGCTCGCTGATGGGTACAGC
ACTGGCACTGGATTGGGTGCTGAGATCATCGGTACCTTTGTTCTTGTCTA
CACTGTTTTCTCCGCCACTGATCCCAAGAGAAATGCTAGGGACTCCCACG
TTCCCGTCTTGGCGCCATTGCCAATTGGATTTGCTGTGTTCATGGTTCAC
TTGGCTACGATCCCTGTCACTGGAACTGGCATCAACCCCGCTAGAAGTTT
GGGAGCTGCAGTGATTTACAGCAAGGAGAAGGCTTGGGATGATCAATGGA
TCTTCTGGGTTGGCCCCTTCATTGGTGCTGCAATTGCTGCATTTTACCAC
CAATTCATCCTCAGAGCATCTGCTTCTAAAGCTCTTGGATCCTTCAAAAG
CCTCCTCCAACATTTAAATTAACTTTTAAAATAATTACTGGAGGATTAAT
CAATGGGTGTCA