BQ623445

Overview
NameBQ623445
Unique NameBQ623445
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length693
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3937 contig Csv1_Contig3937:724..1416. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECOSM (NAD-dependent malic enzyme OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-ETARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLU (NAD-dependent malic enzyme OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLC (NAD-dependent malic enzyme OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECOL6 (NAD-dependent malic enzyme OS=Escherichia coli O6 GN=sfcA PE=3 SV=2)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECOL5 (NAD-dependent malic enzyme OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECOK1 (NAD-dependent malic enzyme OS=Escherichia coli O1:K1 / APEC GN=sfcA PE=3 SV=2)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECOHS (NAD-dependent malic enzyme OS=Escherichia coli O9:H4 (strain HS) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECO8A (NAD-dependent malic enzyme OS=Escherichia coli O8 (strain IAI1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECO81 (NAD-dependent malic enzyme OS=Escherichia coli O81 (strain ED1a) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECO7I (NAD-dependent malic enzyme OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
The following BLAST results are available for this feature:
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_ECOSM1.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli (st... [more]
MAO1_ECOLU1.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O17... [more]
MAO1_ECOLC1.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli (st... [more]
MAO1_ECOL61.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O6 ... [more]
MAO1_ECOL51.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O6:... [more]
MAO1_ECOK11.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O1:... [more]
MAO1_ECOHS1.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O9:... [more]
MAO1_ECO8A1.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O8 ... [more]
MAO1_ECO811.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O81... [more]
MAO1_ECO7I1.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O7:... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00536 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00536 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623445 ID=BQ623445; Name=BQ623445; organism=Citrus sinensis; type=EST; length=693bp
CTGGCCTGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCA
TTTTGGGACTTGGGGATCTTGGCTGTCAGGGGATGGGAATTCCTGTTGGG
AAATTGGCTTTGTACACAGCACTTGGAGGCATTCGTCCCTCAGCATGTTT
GCCCATAACCGTTGATGTGGGTACAAACAATGAGCAATTGTTGAAGGATG
AATTCTACATTGGTCTACGACAGAGGAGGGCAACCGGACAGGAATATGCT
GAACTTCTAGATGAATTCATGAGTGCTGTCAAGCAAAATTATGGAGAAAA
AGTTCTTATACAGTTTGAGGATTTCGCAAACCACAATGCTTTTGAGTTGC
TTGCAAAATATGGCACAACTCATCTTGTCTTCAATGATGATATACAGGGT
ACAGCGTCTGTTGTTCTTGCTGGGGTAGTTGCAGCACTGAAGTTGATTGG
TGGCACACTGGCCGAGCATAGATTTTTGTTTCTTGGAGCTGGGGAGGCTG
GGACTGGTATAGCTGAACTTATTGCGCTTGAGATATCAAAGCAGACTAAA
GCTCCTGTGGAAGAGACCCGGAAGAAGATATGTCTTGTAGACTCACAGGG
ACTGATAGTCAGCTCCCGGAAAGATTCACTTCAACACTTCAAGAAGCCTT
GGGCTCATGAACATGAGCCTGTCAACAATCTCTTAGATGCTGT
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