BQ623445

Overview
NameBQ623445
Unique NameBQ623445
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length693
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3937 contig Csv1_Contig3937:724..1416. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECO5E (NAD-dependent malic enzyme OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECO57 (NAD-dependent malic enzyme OS=Escherichia coli O157:H7 GN=sfcA PE=3 SV=2)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECO45 (NAD-dependent malic enzyme OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECO27 (NAD-dependent malic enzyme OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.589e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_SHISS (NAD-dependent malic enzyme OS=Shigella sonnei (strain Ss046) GN=sfcA PE=3 SV=2)

HSP 1 Score: 185.652 bits (470), Expect = 2.075e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTPREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLI (NAD-dependent malic enzyme OS=Escherichia coli (strain K12) GN=sfcA PE=3 SV=4)

HSP 1 Score: 185.652 bits (470), Expect = 2.075e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECODH (NAD-dependent malic enzyme OS=Escherichia coli (strain K12 / DH10B) GN=sfcA PE=3 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 2.075e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECOBW (NAD-dependent malic enzyme OS=Escherichia coli (strain K12 / BW2952) GN=sfcA PE=3 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 2.075e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECO55 (NAD-dependent malic enzyme OS=Escherichia coli (strain 55989 / EAEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 2.075e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
[Close]
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Match: MAO1_ECO24 (NAD-dependent malic enzyme OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 2.075e-46
Identity = 102/209 (48.80%), Postives = 130/209 (62.20%), Query Frame = 2 
Query:    5 PERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYGTTHLVFNDDIQXXXXXXXXXXXXXXXXXXXTLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKICLVDSQGLIVSSRKDSL 631
            P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY E +DEF+ AVKQ + + VL+QFEDFA  NA  LL +Y      FNDDIQ                    L+E + +FLGAG AG GIAE+I  +  ++  +  E  R+K+ +VD  GL+     + L
Sbjct:  143 PNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD-VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSE-EAARQKVFMVDRFGLLTDKMPNLL 349
The following BLAST results are available for this feature:
BLAST of BQ623445 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_ECO5E1.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O15... [more]
MAO1_ECO571.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O15... [more]
MAO1_ECO451.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O45... [more]
MAO1_ECO271.589e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O12... [more]
MAO1_SHISS2.075e-4648.80NAD-dependent malic enzyme OS=Shigella sonnei (str... [more]
MAO1_ECOLI2.075e-4648.80NAD-dependent malic enzyme OS=Escherichia coli (st... [more]
MAO1_ECODH2.075e-4648.80NAD-dependent malic enzyme OS=Escherichia coli (st... [more]
MAO1_ECOBW2.075e-4648.80NAD-dependent malic enzyme OS=Escherichia coli (st... [more]
MAO1_ECO552.075e-4648.80NAD-dependent malic enzyme OS=Escherichia coli (st... [more]
MAO1_ECO242.075e-4648.80NAD-dependent malic enzyme OS=Escherichia coli O13... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00536 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00536 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623445 ID=BQ623445; Name=BQ623445; organism=Citrus sinensis; type=EST; length=693bp
CTGGCCTGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCA
TTTTGGGACTTGGGGATCTTGGCTGTCAGGGGATGGGAATTCCTGTTGGG
AAATTGGCTTTGTACACAGCACTTGGAGGCATTCGTCCCTCAGCATGTTT
GCCCATAACCGTTGATGTGGGTACAAACAATGAGCAATTGTTGAAGGATG
AATTCTACATTGGTCTACGACAGAGGAGGGCAACCGGACAGGAATATGCT
GAACTTCTAGATGAATTCATGAGTGCTGTCAAGCAAAATTATGGAGAAAA
AGTTCTTATACAGTTTGAGGATTTCGCAAACCACAATGCTTTTGAGTTGC
TTGCAAAATATGGCACAACTCATCTTGTCTTCAATGATGATATACAGGGT
ACAGCGTCTGTTGTTCTTGCTGGGGTAGTTGCAGCACTGAAGTTGATTGG
TGGCACACTGGCCGAGCATAGATTTTTGTTTCTTGGAGCTGGGGAGGCTG
GGACTGGTATAGCTGAACTTATTGCGCTTGAGATATCAAAGCAGACTAAA
GCTCCTGTGGAAGAGACCCGGAAGAAGATATGTCTTGTAGACTCACAGGG
ACTGATAGTCAGCTCCCGGAAAGATTCACTTCAACACTTCAAGAAGCCTT
GGGCTCATGAACATGAGCCTGTCAACAATCTCTTAGATGCTGT
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