BQ623502 | Citrus Genome Database

Overview


Name	BQ623502
Unique Name	BQ623502
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	619

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig7050	contig	Csv1_Contig7050:355..973.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF2_THEEB (Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2 OS=Thermosynechococcus elongatus (strain BP-1) GN=acsF2 PE=3 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 4.983e-64
Identity = 105/168 (62.50%), Postives = 130/168 (77.38%), Query Frame = 2

Query:   68 DKMQGPLRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWARFFCLS 571
            D +    R+ F+++LERSC +EFSGFLL+KEL R+LK  NP++AEIF LM+RDEARHAGFLNK + DF +A+DLG+L+K R YTFF   ++ Y  YLSEKIGYWRYI IYRHL+ +PE   YP+F YFE WCQDENRHGD F AL+ +QPQ +  W AKLW RFF L+
Sbjct:   73 DLLDAQTRKAFIDYLERSCVSEFSGFLLFKELSRKLKDRNPLLAEIFHLMARDEARHAGFLNKAMMDFGHAMDLGYLSKTRTYTFFPLPWVLYTVYLSEKIGYWRYIIIYRHLEKHPEHSFYPLFNYFERWCQDENRHGDIFKALIHSQPQLIQGWGAKLWMRFFLLT 240

HSP 2 Score: 27.335 bits (59), Expect = 4.983e-64
Identity = 10/18 (55.56%), Postives = 13/18 (72.22%), Query Frame = 1

Query:    4 EFKTDYNQTHFVRNKEFK 57
            E + DYN+ HFVR+  FK
Sbjct:   52 EMRADYNRHHFVRDDSFK 69

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF_PROM5 (Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase OS=Prochlorococcus marinus (strain MIT 9515) GN=acsF PE=3 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 6.492e-64
Identity = 112/200 (56.00%), Postives = 140/200 (70.00%), Query Frame = 2

Query:   65 ADKMQGPLRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQ-----------------------------FLNDWK--AKLWARFFCLS 571
            ADK+    R++FV+FLE SCT+EFSGFLLYKEL +R+K  NP++AE F+ M+RDEARHAGFLNK ++DF   LDLGFLT  + YT+F P+ IFYATY+SEKIGYWRYI I+RHL+ NP  + +P+F +FENWCQDENRHGDFF ALMKAQP+                             FLN+    +KLW+RFF L+
Sbjct:   76 ADKLDPETRELFVDFLEGSCTSEFSGFLLYKELSKRIKDKNPLLAECFAHMARDEARHAGFLNKSMNDFGLQLDLGFLTANKDYTYFAPRAIFYATYISEKIGYWRYIAIFRHLEKNPGGKIFPLFNFFENWCQDENRHGDFFDALMKAQPRTVKSLSHKFEIFGYTFKHPIFDYYHRFRYFLNNHPIVSKLWSRFFLLA 275

HSP 2 Score: 35.4242 bits (80), Expect = 6.492e-64
Identity = 14/21 (66.67%), Postives = 18/21 (85.71%), Query Frame = 1

Query:    1 QEFKTDYNQTHFVRNKEFKEA 63
            +EF+ DYN+ HFVRNKEF+ A
Sbjct:   55 EEFRKDYNRHHFVRNKEFEGA 75

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF2_SYNY3 (Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2 OS=Synechocystis sp. (strain PCC 6803) GN=acsF2 PE=3 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 1.105e-63
Identity = 101/175 (57.71%), Postives = 135/175 (77.14%), Query Frame = 2

Query:   56 RKPADKMQGPLRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWARFFCLSGYV 580
            ++  D + G  R  F++FLERSCT+EFSGFLL+KEL RRLK  +P +AE F L++RDEARHAGF+NK ++DF  +LDL +LT+ R YTFF P+++ Y  YLSEKIGYWRYI ++RHL+ NP+   YP+F YFE WCQDENRHGDFF AL+++Q      W+++LW+RFF L+ +V
Sbjct:   73 KRSFDHIDGATRLAFIDFLERSCTSEFSGFLLFKELSRRLKNRSPKLAEAFHLLARDEARHAGFINKAMADFGLSLDLRYLTQKRTYTFFPPEWVIYTVYLSEKIGYWRYILMFRHLEKNPDHNIYPLFNYFECWCQDENRHGDFFKALLRSQTALWKTWQSRLWSRFFLLTVFV 247

HSP 2 Score: 31.187 bits (69), Expect = 1.105e-63
Identity = 11/21 (52.38%), Postives = 16/21 (76.19%), Query Frame = 1

Query:    1 QEFKTDYNQTHFVRNKEFKEA 63
            +E + DYN+ HFVRN +FK +
Sbjct:   55 EELRADYNRYHFVRNDDFKRS 75

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF_RHOP2 (Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase OS=Rhodopseudomonas palustris (strain HaA2) GN=acsF PE=3 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 6.013e-53
Identity = 98/168 (58.33%), Postives = 124/168 (73.81%), Query Frame = 2

Query:   68 DKMQGPLRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWARFFCLS 571
            DK+   LR  F +FL  S TAEFSG +LY E+ +R+K  NP + E+F L+SRDEARHAGF+N+ L D    +DL FLTK +KYT+F+PKFIFYATYLSEKIGY RYITIYR ++ +PE + +PIFK+FE WC DE RHG+ F+ LM+A P  L+    KLW RFF L+
Sbjct:   86 DKLPPALRAEFKDFLVSSLTAEFSGCVLYAEIKKRIK--NPEIRELFGLLSRDEARHAGFINEILKDHGIGVDLSFLTKVKKYTYFRPKFIFYATYLSEKIGYARYITIYRQMERHPERRFHPIFKWFERWCNDEFRHGEAFALLMRADPSLLSGVN-KLWIRFFLLA 250

HSP 2 Score: 28.4906 bits (62), Expect = 6.013e-53
Identity = 10/20 (50.00%), Postives = 15/20 (75.00%), Query Frame = 1

Query:    4 EFKTDYNQTHFVRNKEFKEA 63
            E + DYN++HF +N EF E+
Sbjct:   64 EMRADYNKSHFKKNDEFLES 83

HSP 3 Score: 21.557 bits (44), Expect = 6.013e-53
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 1

Query:  559 FLPLGVCTMYLNDCQRTAFY 618
            FL     TMY+ D  R AFY
Sbjct:  247 FLLAVFATMYVRDHMRPAFY 266

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF_BRASB (Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase OS=Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) GN=acsF PE=3 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 7.448e-53
Identity = 101/176 (57.39%), Postives = 128/176 (72.73%), Query Frame = 2

Query:   44 TRSSRKPADKMQGPLRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWARFFCLS 571
            T    K  +++   LR  F +FL  S TAEFSG +LY E+ +R+  TNP + E+FS MSRDEARHAGF+N+ L D    +DLGFLTK +KYT+FKPKFIFYATYLSEKIGY RYITIYR ++ +PE Q +PIFK+FE WC DE RHG+ F+ LM+A P+ L+    KLW +FF L+
Sbjct:   65 TPEFEKDLNELPEALRLEFKDFLVSSLTAEFSGCVLYAEIKKRI--TNPDIRELFSYMSRDEARHAGFINEILKDHGIGVDLGFLTKVKKYTYFKPKFIFYATYLSEKIGYARYITIYRQMERHPERQFHPIFKWFERWCNDEFRHGEAFALLMRADPKLLSGLN-KLWIKFFLLA 237

HSP 2 Score: 26.1794 bits (56), Expect = 7.448e-53
Identity = 9/19 (47.37%), Postives = 14/19 (73.68%), Query Frame = 1

Query:    4 EFKTDYNQTHFVRNKEFKE 60
            E + D+N+ HFVR  EF++
Sbjct:   52 ELRADHNRKHFVRTPEFEK 70

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF_BRASO (Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase OS=Bradyrhizobium sp. (strain ORS278) GN=acsF PE=3 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 9.708e-53
Identity = 99/171 (57.89%), Postives = 127/171 (74.27%), Query Frame = 2

Query:   59 KPADKMQGPLRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWARFFCLS 571
            K  +++   LR  F +FL  S TAEFSG +LY E+ +R+  TNP + E+F  MSRDEARHAGF+N+ L D    +DLGFLTK +KYT+FKPKFIFYATYLSEKIGY RYITIYR ++ +PE Q +PIFK+FE WC DE RHG+ F+ LM+A P+ L+ +  KLW +FF L+
Sbjct:   70 KDLNELPEALRAEFKDFLVSSLTAEFSGCVLYAEIKKRI--TNPDIRELFQFMSRDEARHAGFINEILKDHGIGVDLGFLTKTKKYTYFKPKFIFYATYLSEKIGYARYITIYRQMERHPERQFHPIFKWFERWCNDEFRHGEAFALLMRADPKLLSGYN-KLWIKFFLLA 237

HSP 2 Score: 25.409 bits (54), Expect = 9.708e-53
Identity = 9/17 (52.94%), Postives = 12/17 (70.59%), Query Frame = 1

Query:    4 EFKTDYNQTHFVRNKEF 54
            E + D+N+ HFVR  EF
Sbjct:   52 ELRADHNRKHFVRTPEF 68

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF_RHOPT (Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase OS=Rhodopseudomonas palustris (strain TIE-1) GN=acsF PE=3 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 1.089e-51
Identity = 98/168 (58.33%), Postives = 124/168 (73.81%), Query Frame = 2

Query:   68 DKMQGPLRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWARFFCLS 571
            DK+   LR  F +FL  S TAEFSG +LY E+ +R+K  NP + E+F L+SRDEARHAGF+N+ L D    +DL FLTK +KYT+F+PKFIFYATYLSEKIGY RYITIYR ++ +PE + +PIFK+FE WC DE RHG+ F+ LM+A P  L+    KLW RFF L+
Sbjct:   86 DKLPPELRAEFKDFLVSSLTAEFSGCVLYAEIKKRIK--NPEIRELFGLLSRDEARHAGFINEILKDHGIGVDLSFLTKVKKYTYFRPKFIFYATYLSEKIGYARYITIYRQMERHPERRFHPIFKWFERWCNDEFRHGEAFALLMRADPSLLSGVN-KLWIRFFLLA 250

HSP 2 Score: 24.6386 bits (52), Expect = 1.089e-51
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = 1

Query:    4 EFKTDYNQTHFVRNKEF 54
            E + DYN++HF +  EF
Sbjct:   64 ELRADYNKSHFKKTDEF 80

HSP 3 Score: 21.1718 bits (43), Expect = 1.089e-51
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 1

Query:  559 FLPLGVCTMYLNDCQRTAFY 618
            FL     TMY+ D  R AFY
Sbjct:  247 FLLAVFSTMYVRDHMRPAFY 266

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF_RHOPA (Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase OS=Rhodopseudomonas palustris GN=acsF PE=3 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 1.089e-51
Identity = 98/168 (58.33%), Postives = 124/168 (73.81%), Query Frame = 2

Query:   68 DKMQGPLRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWARFFCLS 571
            DK+   LR  F +FL  S TAEFSG +LY E+ +R+K  NP + E+F L+SRDEARHAGF+N+ L D    +DL FLTK +KYT+F+PKFIFYATYLSEKIGY RYITIYR ++ +PE + +PIFK+FE WC DE RHG+ F+ LM+A P  L+    KLW RFF L+
Sbjct:   86 DKLPPELRAEFKDFLVSSLTAEFSGCVLYAEIKKRIK--NPEIRELFGLLSRDEARHAGFINEILKDHGIGVDLSFLTKVKKYTYFRPKFIFYATYLSEKIGYARYITIYRQMERHPERRFHPIFKWFERWCNDEFRHGEAFALLMRADPSLLSGVN-KLWIRFFLLA 250

HSP 2 Score: 24.6386 bits (52), Expect = 1.089e-51
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = 1

Query:    4 EFKTDYNQTHFVRNKEF 54
            E + DYN++HF +  EF
Sbjct:   64 ELRADYNKSHFKKTDEF 80

HSP 3 Score: 21.1718 bits (43), Expect = 1.089e-51
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 1

Query:  559 FLPLGVCTMYLNDCQRTAFY 618
            FL     TMY+ D  R AFY
Sbjct:  247 FLLAVFSTMYVRDHMRPAFY 266

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF_RHOPS (Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase OS=Rhodopseudomonas palustris (strain BisB5) GN=acsF PE=3 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 4.061e-51
Identity = 97/168 (57.74%), Postives = 122/168 (72.62%), Query Frame = 2

Query:   68 DKMQGPLRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWARFFCLS 571
            DK+   L   F +FL  S TAEFSG +LY E+ +R+K  NP + E+F L+SRDEARHAGF+N+ L D    +DL FLTK +KYT+F+PKFIFYATYLSEKIGY RYITIYR ++ +PE + +PIFK+FE WC DE RHG+ F+ LM+A P  L     KLW RFF L+
Sbjct:   88 DKLPPELLIEFKDFLVSSLTAEFSGCVLYSEIKKRIK--NPEIRELFGLLSRDEARHAGFINEILKDHGIGVDLSFLTKVKKYTYFRPKFIFYATYLSEKIGYARYITIYRQMERHPERRFHPIFKWFERWCNDEFRHGEAFALLMRADPSLLRGVN-KLWIRFFLLA 252

HSP 2 Score: 25.7942 bits (55), Expect = 4.061e-51
Identity = 9/20 (45.00%), Postives = 13/20 (65.00%), Query Frame = 1

Query:    4 EFKTDYNQTHFVRNKEFKEA 63
            E + DYN+ HF +  EF E+
Sbjct:   66 EMRADYNKAHFKKTDEFLES 85

HSP 3 Score: 21.557 bits (44), Expect = 4.061e-51
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 1

Query:  559 FLPLGVCTMYLNDCQRTAFY 618
            FL     TMY+ D  R AFY
Sbjct:  249 FLLAVFATMYVRDHMRPAFY 268

[Close]

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Match: ACSF_RHOGE (Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase OS=Rhodocyclus gelatinosus GN=acsF PE=3 SV=2)

HSP 1 Score: 175.637 bits (444), Expect = 1.732e-43
Identity = 83/164 (50.61%), Postives = 118/164 (71.95%), Query Frame = 2

Query:   86 LRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLSDFNYALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEFQCYPIFKYFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWARFFCLSGY 577
            LRQ F++FL  S T+EFSG +LY E+ + ++  NP V  +   M+R+E+RHAGF+N+ L DF   ++LG L + + YT+FKPK+IFYATYLSEKIGY RYITIYR L+ +P+ + +PIF++FE WC DE RHG+ F+ +++A P  ++     LW RFF L+ Y
Sbjct:   84 LRQEFLDFLVSSVTSEFSGCVLYNEIQKNVE--NPDVKALMRYMARNESRHAGFINQALRDFGLGINLGGLKRTKAYTYFKPKYIFYATYLSEKIGYARYITIYRQLERHPDKRFHPIFRWFERWCNDEFRHGESFALILRAHPHLISGANL-LWVRFFLLAVY 244

The following BLAST results are available for this feature:

BLAST of BQ623502 vs. ExPASy Swiss-Prot

BLAST of BQ623502 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 44


Match Name	E-value	Identity	Description
ACSF2_THEEB	4.983e-64	62.50	Magnesium-protoporphyrin IX monomethyl ester [oxid...	[more]
ACSF_PROM5	6.492e-64	56.00	Magnesium-protoporphyrin IX monomethyl ester [oxid...	[more]
ACSF2_SYNY3	1.105e-63	57.71	Magnesium-protoporphyrin IX monomethyl ester [oxid...	[more]
ACSF_RHOP2	6.013e-53	58.33	Aerobic magnesium-protoporphyrin IX monomethyl est...	[more]
ACSF_BRASB	7.448e-53	57.39	Aerobic magnesium-protoporphyrin IX monomethyl est...	[more]
ACSF_BRASO	9.708e-53	57.89	Aerobic magnesium-protoporphyrin IX monomethyl est...	[more]
ACSF_RHOPT	1.089e-51	58.33	Aerobic magnesium-protoporphyrin IX monomethyl est...	[more]
ACSF_RHOPA	1.089e-51	58.33	Aerobic magnesium-protoporphyrin IX monomethyl est...	[more]
ACSF_RHOPS	4.061e-51	57.74	Aerobic magnesium-protoporphyrin IX monomethyl est...	[more]
ACSF_RHOGE	1.732e-43	50.61	Aerobic magnesium-protoporphyrin IX monomethyl est...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_00593 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00593 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623502 ID=BQ623502; Name=BQ623502; organism=Citrus sinensis; type=EST; length=619bp
CAAGAATTCAAGACTGATTATAACCAGACTCACTTTGTGAGGAACAAGGA
GTTCAAGGAAGCCTGCTGACAAGATGCAAGGCCCTCTCAGGCAGATCTTT
GTTGAGTTCTTGGAGAGGTCTTGCACTGCTGAGTTCTCTGGTTTCCTTCT
TTACAAAGAGCTCGGAAGGAGACTCAAGAAAACCAACCCAGTGGTAGCTG
AGATTTTCTCTCTGATGTCCAGGGATGAAGCCAGGCATGCCGGGTTTCTG
AACAAGGGGCTATCTGATTTCAATTATGCATTGGACTTGGGCTTCCTGAC
AAAAGCTAGGAAATACACATTTTTCAAGCCAAAGTTTATCTTTTATGCCA
CATATTTATCTGAGAAGATTGGATACTGGAGATACATTACCATATACAGA
CATCTCAAGGCCAACCCTGAGTTCCAATGTTATCCCATTTTCAAGTATTT
TGAGAACTGGTGCCAGGATGAGAACCGTCACGGTGATTTCTTCTCAGCCT
TGATGAAGGCACAACCCCAGTTCCTCAATGACTGGAAGGCAAAGTTGTGG
GCCCGATTTTTCTGCCTCTCGGGGTATGTACAATGTATCTCAATGACTGC
CAGCGTACTGCTTTCTATG