BQ623508

Overview
NameBQ623508
Unique NameBQ623508
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length649
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2169 contig Csv1_Contig2169:929..1577. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_WHEAT (Elongation factor 1-alpha OS=Triticum aestivum GN=TEF1 PE=2 SV=1)

HSP 1 Score: 266.159 bits (679), Expect = 6.042e-77
Identity = 131/141 (92.91%), Postives = 136/141 (96.45%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRGFVASNSKDDPA+EAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+ELVTKIDRRSGKELE  PKFLKNGDAG+VKMIPTKPMVVETF+ YPPLGRFAVRDMRQTVAVGVIK VEKKDPTG
Sbjct:  296 VGFNVKNVAVKDLKRGFVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKELEALPKFLKNGDAGIVKMIPTKPMVVETFATYPPLGRFAVRDMRQTVAVGVIKGVEKKDPTG 436

HSP 2 Score: 42.743 bits (99), Expect = 6.042e-77
Identity = 19/21 (90.48%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE+L EALPGDNVG
Sbjct:  277 VKSVEMHHESLLEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A1_DAUCA (Elongation factor 1-alpha OS=Daucus carota PE=3 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 8.606e-76
Identity = 127/141 (90.07%), Postives = 138/141 (97.87%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNV+VKDLKRG+VASNSKDDPA+ AA+FTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+EL+TKIDRRSGKELEKEPKFLKNGDAGMVKM+PTKPMVVETF++YPPLGRFAVR MRQTVAVGVIK+VEKKDPTG
Sbjct:  296 VGFNVKNVSVKDLKRGYVASNSKDDPAKGAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELLTKIDRRSGKELEKEPKFLKNGDAGMVKMLPTKPMVVETFAEYPPLGRFAVRVMRQTVAVGVIKAVEKKDPTG 436

HSP 2 Score: 42.743 bits (99), Expect = 8.606e-76
Identity = 19/21 (90.48%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE+L EALPGDNVG
Sbjct:  277 VKSVEMHHESLLEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A2_HORVU (Elongation factor 1-alpha OS=Hordeum vulgare GN=BLT63 PE=1 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 7.205e-75
Identity = 126/141 (89.36%), Postives = 134/141 (95.04%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRG+VASNSKDDPA+EAANFT+QVIIMNHPGQI NGYAPVLDCHTSHIAVKF+E+ TKIDRRSGKELE  PKFLKNGDAG VKMIPTKPMVVETF+ YPPLGRFAVRDMRQTVAVGVIKSVEKK+PTG
Sbjct:  296 VGFNVKNVAVKDLKRGYVASNSKDDPAKEAANFTAQVIIMNHPGQISNGYAPVLDCHTSHIAVKFAEIQTKIDRRSGKELEAAPKFLKNGDAGFVKMIPTKPMVVETFAQYPPLGRFAVRDMRQTVAVGVIKSVEKKEPTG 436

HSP 2 Score: 42.743 bits (99), Expect = 7.205e-75
Identity = 19/21 (90.48%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE+L EALPGDNVG
Sbjct:  277 VKSVEMHHESLLEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A1_HORVU (Elongation factor 1-alpha OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 2.085e-74
Identity = 126/141 (89.36%), Postives = 134/141 (95.04%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRG+VASNSKDDPA+EAANFT+QVIIMNHPGQI NGYAPVLDCHTSHIAVKF+E+ TKIDRRSGKELE  PKFLKNGDAG VKMIPTKPMVVETF+ YPPLGRFAVRDMRQTVAVGVIKSVEKK+PTG
Sbjct:  296 VGFNVKNVAVKDLKRGYVASNSKDDPAKEAANFTAQVIIMNHPGQISNGYAPVLDCHTSHIAVKFAEIQTKIDRRSGKELEAAPKFLKNGDAGFVKMIPTKPMVVETFAQYPPLGRFAVRDMRQTVAVGVIKSVEKKEPTG 436

HSP 2 Score: 41.2022 bits (95), Expect = 2.085e-74
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVE+HHE+L EALPGDNVG
Sbjct:  277 VKSVEVHHESLLEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A2_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-2 PE=3 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 1.566e-62
Identity = 102/141 (72.34%), Postives = 126/141 (89.36%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNV+VKD++RG V S+SK+DPA+E+A+FT+QVII+NHPGQI  GYAPVLDCHT+HIA KFSEL+ KIDRRSGK++E  PKF+K+GD+ +VKM+P+KPM VE ++DYPPLGRFAVRDMRQTVAVGVIK+VEK D  G
Sbjct:  306 VGFNVKNVSVKDIRRGNVCSDSKNDPAKESASFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFSELIEKIDRRSGKKMEDSPKFVKSGDSAIVKMVPSKPMCVEAYTDYPPLGRFAVRDMRQTVAVGVIKAVEKVDKAG 446

HSP 2 Score: 41.2022 bits (95), Expect = 1.566e-62
Identity = 18/21 (85.71%), Postives = 18/21 (85.71%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE L E LPGDNVG
Sbjct:  287 VKSVEMHHETLTEGLPGDNVG 307
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A1_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-1 PE=3 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 1.566e-62
Identity = 102/141 (72.34%), Postives = 126/141 (89.36%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNV+VKD++RG V S+SK+DPA+E+A+FT+QVII+NHPGQI  GYAPVLDCHT+HIA KFSEL+ KIDRRSGK++E  PKF+K+GD+ +VKM+P+KPM VE ++DYPPLGRFAVRDMRQTVAVGVIK+VEK D  G
Sbjct:  306 VGFNVKNVSVKDIRRGNVCSDSKNDPAKESASFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFSELIEKIDRRSGKKMEDSPKFVKSGDSAIVKMVPSKPMCVEAYTDYPPLGRFAVRDMRQTVAVGVIKAVEKVDKAG 446

HSP 2 Score: 41.2022 bits (95), Expect = 1.566e-62
Identity = 18/21 (85.71%), Postives = 18/21 (85.71%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE L E LPGDNVG
Sbjct:  287 VKSVEMHHETLTEGLPGDNVG 307
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_ABSGL (Elongation factor 1-alpha OS=Absidia glauca GN=TEF-1 PE=3 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 2.042e-62
Identity = 103/141 (73.05%), Postives = 126/141 (89.36%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNV+VKD++RG V S+SK+DPA+EA +FT+QVI++NHPGQIG GYAPVLDCHT+HIA KF+EL+ KIDRRSGK+LE  PKF+K+GD+ +VKMIP+KPM VE ++DYPPLGRFAVRDMRQTVAVGVIK+VEK D  G
Sbjct:  306 VGFNVKNVSVKDIRRGNVCSDSKNDPAKEAGSFTAQVIVLNHPGQIGAGYAPVLDCHTAHIACKFAELLEKIDRRSGKKLEDAPKFVKSGDSAIVKMIPSKPMCVEAYTDYPPLGRFAVRDMRQTVAVGVIKAVEKVDKAG 446

HSP 2 Score: 40.4318 bits (93), Expect = 2.042e-62
Identity = 18/21 (85.71%), Postives = 18/21 (85.71%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE L E LPGDNVG
Sbjct:  287 VKSVEMHHEQLVEGLPGDNVG 307
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_TRYBB (Elongation factor 1-alpha OS=Trypanosoma brucei brucei GN=TEF1 PE=1 SV=3)

HSP 1 Score: 220.32 bits (560), Expect = 2.042e-62
Identity = 103/141 (73.05%), Postives = 125/141 (88.65%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNV+VKD++RG V  N+K+DP +EAA+FT+QVII+NHPGQIGNGYAPVLDCHTSHIA KF+E+ +KIDRRSGKELEK PK +K+GDA +V+M+P KPM VE F+DY PLGRFAVRDMRQTVAVG+IK+V KKD +G
Sbjct:  296 VGFNVKNVSVKDIRRGNVCGNTKNDPPKEAADFTAQVIILNHPGQIGNGYAPVLDCHTSHIACKFAEIESKIDRRSGKELEKAPKSIKSGDAAIVRMVPQKPMCVEVFNDYAPLGRFAVRDMRQTVAVGIIKAVTKKDGSG 436

HSP 2 Score: 40.0466 bits (92), Expect = 2.042e-62
Identity = 17/21 (80.95%), Postives = 18/21 (85.71%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKS+EMHHE L EA PGDNVG
Sbjct:  277 VKSIEMHHEQLAEATPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A0_XENLA (Elongation factor 1-alpha, somatic form OS=Xenopus laevis GN=eef1as PE=2 SV=1)

HSP 1 Score: 216.083 bits (549), Expect = 4.526e-62
Identity = 105/137 (76.64%), Postives = 121/137 (88.32%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 469
            + FNVKNV+VKD++RG VA +SK+DP  EA +FT+QVII+NHPGQIG GYAPVLDCHT+HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V MIP KPM VE+FSDYPPLGRFAVRDMRQTVAVGVIK+VEKK
Sbjct:  308 VGFNVKNVSVKDVRRGNVAGDSKNDPPMEAGSFTAQVIILNHPGQIGAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDNPKFLKSGDAAIVDMIPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVEKK 444

HSP 2 Score: 43.1282 bits (100), Expect = 4.526e-62
Identity = 19/21 (90.48%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEAL EA+PGDNVG
Sbjct:  289 VKSVEMHHEALTEAVPGDNVG 309
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A2_XENLA (Elongation factor 1-alpha, oocyte form OS=Xenopus laevis GN=eef1ao PE=1 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 4.527e-62
Identity = 101/137 (73.72%), Postives = 121/137 (88.32%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 469
            + FNVKN++VKD++RG VA +SK+DP  +A +FT+QVII+NHPGQI  GYAPVLDCHT+HIA KF+EL  KIDRRSGK+LE +PKFLK+GDA +V+MIP KPM VE+FSDYPPLGRFAVRDMRQTVAVGVIK V+KK
Sbjct:  308 VGFNVKNISVKDIRRGNVAGDSKNDPPMQAGSFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKQKIDRRSGKKLEDDPKFLKSGDAAIVEMIPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKGVDKK 444

HSP 2 Score: 46.595 bits (109), Expect = 4.527e-62
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEALPGDNVG
Sbjct:  289 VKSVEMHHEALQEALPGDNVG 309
The following BLAST results are available for this feature:
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 171
Match NameE-valueIdentityDescription
EF1A_WHEAT6.042e-7792.91Elongation factor 1-alpha OS=Triticum aestivum GN=... [more]
EF1A1_DAUCA8.606e-7690.07Elongation factor 1-alpha OS=Daucus carota PE=3 SV... [more]
EF1A2_HORVU7.205e-7589.36Elongation factor 1-alpha OS=Hordeum vulgare GN=BL... [more]
EF1A1_HORVU2.085e-7489.36Elongation factor 1-alpha OS=Hordeum vulgare PE=1 ... [more]
EF1A2_RHIRA1.566e-6272.34Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A1_RHIRA1.566e-6272.34Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A_ABSGL2.042e-6273.05Elongation factor 1-alpha OS=Absidia glauca GN=TEF... [more]
EF1A_TRYBB2.042e-6273.05Elongation factor 1-alpha OS=Trypanosoma brucei br... [more]
EF1A0_XENLA4.526e-6276.64Elongation factor 1-alpha, somatic form OS=Xenopus... [more]
EF1A2_XENLA4.527e-6273.72Elongation factor 1-alpha, oocyte form OS=Xenopus ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00599 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00599 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623508 ID=BQ623508; Name=BQ623508; organism=Citrus sinensis; type=EST; length=649bp
GTTAAGTCTGTTGAGATGCACCACGAAGCTCTGCAGGAGGCCCTTCCGGG
TGACAATGTTGGCCTTCAACGTGAAGAACGTTGCTGTCAAGGATCTCAAG
CGTGGTTTTGTTGCTTCCAACTCGAAGGATGATCCGGCCAGGGAAGCTGC
CAACTTCACTTCACAGGTCATCATCATGAACCATCCTGGCCAGATTGGAA
ATGGATATGCCCCAGTGCTTGACTGCCACACCTCCCACATTGCCGTCAAG
TTTTCTGAGCTCGTGACGAAGATTGATAGGCGATCAGGCAAGGAGCTTGA
GAAGGAGCCCAAGTTCTTGAAGAATGGAGATGCCGGAATGGTTAAGATGA
TTCCCACCAAGCCCATGGTTGTTGAGACATTCTCCGACTACCCACCCCTT
GGTCGTTTTGCTGTTAGGGACATGCGTCAGACTGTTGCTGTGGGTGTTAT
CAAGAGTGTTGAGAAGAAGGATCCCACAGGTGCCAAGGTCACCAAGTCCG
CAGCCAAGAAGAAATGAGAAATTTTTATGGGTCTGTCTACAATAGTTAGG
GTTTATTTTCTGGCTTTTTTATTTTCTTTTCTGGTTTTGGTTTGCATCAA
TGTTTCTGCAAACAGCCCTATTTGCTTCCACCAGTTTGCTGGTGAGCAT
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