BQ623508

Overview
NameBQ623508
Unique NameBQ623508
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length649
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2169 contig Csv1_Contig2169:929..1577. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_TETPY (Elongation factor 1-alpha OS=Tetrahymena pyriformis PE=2 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 1.177e-56
Identity = 95/138 (68.84%), Postives = 115/138 (83.33%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKD 472
            + FN+K V+VKD++RG VAS++K+DPA+EAA F SQVIIMNHPGQI  GY PVLDCHT+HIA KF  +  KIDRR+GK  E+ PKF+KNGDA +V +IPTK + VE F +YPPLGR+AVRDM+QTVAVGVIK VEKKD
Sbjct:  297 VGFNIKGVSVKDIRRGNVASDAKNDPAKEAATFYSQVIIMNHPGQIQAGYTPVLDCHTAHIACKFETIHDKIDRRTGKSQEENPKFIKNGDAALVTLIPTKALCVEVFQEYPPLGRYAVRDMKQTVAVGVIKKVEKKD 434

HSP 2 Score: 39.6614 bits (91), Expect = 1.177e-56
Identity = 17/20 (85.00%), Postives = 18/20 (90.00%), Query Frame = 1 
Query:    4 KSVEMHHEALQEALPGDNVG 63
            KSVEMHHE L EA+PGDNVG
Sbjct:  279 KSVEMHHEQLPEAVPGDNVG 298
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_YEAST (Elongation factor 1-alpha OS=Saccharomyces cerevisiae GN=TEF1 PE=1 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 1.669e-55
Identity = 91/138 (65.94%), Postives = 116/138 (84.06%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKD 472
            + FNVKNV+VK+++RG V  ++K+DP +  A+F + VI++NHPGQI  GY+PVLDCHT+HIA +F EL+ K DRRSGK+LE  PKFLK+GDA +VK +P+KPM VE FS+YPPLGRFAVRDMRQTVAVGVIKSV+K +
Sbjct:  306 VGFNVKNVSVKEIRRGNVCGDAKNDPPKGCASFNATVIVLNHPGQISAGYSPVLDCHTAHIACRFDELLEKNDRRSGKKLEDHPKFLKSGDAALVKFVPSKPMCVEAFSEYPPLGRFAVRDMRQTVAVGVIKSVDKTE 443

HSP 2 Score: 39.6614 bits (91), Expect = 1.669e-55
Identity = 16/21 (76.19%), Postives = 19/21 (90.48%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE L++ +PGDNVG
Sbjct:  287 VKSVEMHHEQLEQGVPGDNVG 307
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A1_EUPCR (Elongation factor 1-alpha 1 OS=Euplotes crassus GN=EFA1 PE=3 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 1.162e-53
Identity = 89/137 (64.96%), Postives = 114/137 (83.21%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 469
            + FNVK ++VKD+KRGFVAS+SK+DPA +  +F S  I+MNHPG+I  GY PV+DCHT+HIA KF EL+TK D+RSGK  E+ PKFLK GDAG++++ P+KP+ VETF+ Y PLGRFAVRDMRQTVAVGVI+ ++KK
Sbjct:  296 VGFNVKGLSVKDIKRGFVASDSKNDPATDTESFVSHTIVMNHPGEIKAGYTPVIDCHTAHIACKFEELLTKADKRSGKMTEENPKFLKAGDAGLIRLSPSKPLCVETFATYAPLGRFAVRDMRQTVAVGVIQEIKKK 432

HSP 2 Score: 35.8094 bits (81), Expect = 1.162e-53
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 1 
Query:    4 KSVEMHHEALQEALPGDNVG 63
            KSVEMHHE + EA PG+NVG
Sbjct:  278 KSVEMHHEEVPEAAPGNNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_DICDI (Elongation factor 1-alpha OS=Dictyostelium discoideum GN=eef1a1 PE=1 SV=2)

HSP 1 Score: 189.889 bits (481), Expect = 2.573e-53
Identity = 91/142 (64.08%), Postives = 115/142 (80.99%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKE---PKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDP 475
            + FNVKNV+VK++KRG VA +SK+DP +E   F +QVI++NHPGQI  GY+PVLDCHT+HIA KF+E+V K+DRR+G  + KE      LKNGDA MV++ P++PM VE+F++YPPLGRFAVRDMRQTVAVGVIKS  KK P
Sbjct:  296 VGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAP 437

HSP 2 Score: 40.0466 bits (92), Expect = 2.573e-53
Identity = 18/21 (85.71%), Postives = 18/21 (85.71%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE L EA PGDNVG
Sbjct:  277 VKSVEMHHEQLPEARPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_PLAFK (Elongation factor 1-alpha OS=Plasmodium falciparum (isolate K1 / Thailand) GN=MEF-1 PE=3 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 5.700e-53
Identity = 84/138 (60.87%), Postives = 119/138 (86.23%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKD 472
            + FNVKNV+VK++KRG+VAS++K++PA+  + FT+QVII+NHPG+I NGY P+LDCHTSHI+ KF  + +KID+RSGK +E+ PK +K+GD+ +V + P KPMVVETF++YPPLGRFA+RDMRQT+AVG+I  +++K+
Sbjct:  294 IGFNVKNVSVKEIKRGYVASDTKNEPAKGCSKFTAQVIILNHPGEIKNGYTPLLDCHTSHISCKFLNIDSKIDKRSGKVVEENPKAIKSGDSALVSLEPKKPMVVETFTEYPPLGRFAIRDMRQTIAVGIINQLKRKN 431

HSP 2 Score: 36.1946 bits (82), Expect = 5.700e-53
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 1 
Query:    4 KSVEMHHEALQEALPGDNVG 63
            KSVEMH E L+EA PGDN+G
Sbjct:  276 KSVEMHKEVLEEARPGDNIG 295
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_ENTHI (Elongation factor 1-alpha OS=Entamoeba histolytica PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 9.689e-53
Identity = 88/135 (65.19%), Postives = 115/135 (85.19%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELE-KEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSV 460
            + FNV+N+ VKD+KRG VAS++K+ PA    +FT+QVI+MNHPGQI  GY PVLDCHTSHIA KF EL++KIDRR+GK +E  EP+++KNGD+ +VK++PTKP+ VE F+ +PPLGRFAVRDM+QTVAVGV+K+V
Sbjct:  294 VGFNVRNLTVKDIKRGNVASDAKNQPAVGCEDFTAQVIVMNHPGQIRKGYTPVLDCHTSHIACKFEELLSKIDRRTGKSMEGGEPEYIKNGDSALVKIVPTKPLCVEEFAKFPPLGRFAVRDMKQTVAVGVVKAV 428

HSP 2 Score: 38.1206 bits (87), Expect = 9.689e-53
Identity = 15/20 (75.00%), Postives = 18/20 (90.00%), Query Frame = 1 
Query:    4 KSVEMHHEALQEALPGDNVG 63
            KS+EMHH AL +A+PGDNVG
Sbjct:  276 KSIEMHHTALAQAIPGDNVG 295
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A1_XENLA (Elongation factor 1-alpha OS=Xenopus laevis PE=1 SV=2)

HSP 1 Score: 187.578 bits (475), Expect = 1.788e-51
Identity = 89/137 (64.96%), Postives = 109/137 (79.56%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 469
            + FNVKN+AVK LKRG VA NSK DP  EA++FT+QVII+NHPG I  GY+PV+DCHT+HI  +F+EL  KIDRR+GK+LE  P  LK+GDA ++ + P KP  VE F DYPPLGRFA RD++QTVAVGV+KSVE K
Sbjct:  311 IGFNVKNIAVKSLKRGNVAGNSKSDPPTEASSFTAQVIILNHPGFIKAGYSPVIDCHTAHITCQFAELQEKIDRRTGKKLEDNPGLLKSGDAAIITLKPIKPFCVERFFDYPPLGRFAARDLKQTVAVGVVKSVEHK 447

HSP 2 Score: 36.1946 bits (82), Expect = 1.788e-51
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKS+EMHHE LQ A PG N+G
Sbjct:  292 VKSIEMHHEPLQMAFPGFNIG 312
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_SPOFR (Elongation factor 1-alpha (Fragment) OS=Spodoptera frugiperda PE=1 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 2.752e-49
Identity = 74/120 (61.67%), Postives = 102/120 (85.00%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVR 418
            + FNVKNV+VK+L+RG+VA +SK++P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRR+GK  E  PK +K+GDA +V ++P+KP+ VE+F ++PPLGRFAVR
Sbjct:  294 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 413

HSP 2 Score: 45.4394 bits (106), Expect = 2.752e-49
Identity = 20/21 (95.24%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEA+PGDNVG
Sbjct:  275 VKSVEMHHEALQEAVPGDNVG 295
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_HELZE (Elongation factor 1-alpha (Fragment) OS=Heliothis zea PE=3 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 2.752e-49
Identity = 74/120 (61.67%), Postives = 102/120 (85.00%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVR 418
            + FNVKNV+VK+L+RG+VA +SK++P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRR+GK  E  PK +K+GDA +V ++P+KP+ VE+F ++PPLGRFAVR
Sbjct:  294 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 413

HSP 2 Score: 45.4394 bits (106), Expect = 2.752e-49
Identity = 20/21 (95.24%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEA+PGDNVG
Sbjct:  275 VKSVEMHHEALQEAVPGDNVG 295
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_HELVI (Elongation factor 1-alpha (Fragment) OS=Heliothis virescens PE=3 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 2.752e-49
Identity = 74/120 (61.67%), Postives = 102/120 (85.00%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVR 418
            + FNVKNV+VK+L+RG+VA +SK++P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRR+GK  E  PK +K+GDA +V ++P+KP+ VE+F ++PPLGRFAVR
Sbjct:  294 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 413

HSP 2 Score: 45.4394 bits (106), Expect = 2.752e-49
Identity = 20/21 (95.24%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEA+PGDNVG
Sbjct:  275 VKSVEMHHEALQEAVPGDNVG 295
The following BLAST results are available for this feature:
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 171
Match NameE-valueIdentityDescription
EF1A_TETPY1.177e-5668.84Elongation factor 1-alpha OS=Tetrahymena pyriformi... [more]
EF1A_YEAST1.669e-5565.94Elongation factor 1-alpha OS=Saccharomyces cerevis... [more]
EF1A1_EUPCR1.162e-5364.96Elongation factor 1-alpha 1 OS=Euplotes crassus GN... [more]
EF1A_DICDI2.573e-5364.08Elongation factor 1-alpha OS=Dictyostelium discoid... [more]
EF1A_PLAFK5.700e-5360.87Elongation factor 1-alpha OS=Plasmodium falciparum... [more]
EF1A_ENTHI9.689e-5365.19Elongation factor 1-alpha OS=Entamoeba histolytica... [more]
EF1A1_XENLA1.788e-5164.96Elongation factor 1-alpha OS=Xenopus laevis PE=1 S... [more]
EF1A_SPOFR2.752e-4961.67Elongation factor 1-alpha (Fragment) OS=Spodoptera... [more]
EF1A_HELZE2.752e-4961.67Elongation factor 1-alpha (Fragment) OS=Heliothis ... [more]
EF1A_HELVI2.752e-4961.67Elongation factor 1-alpha (Fragment) OS=Heliothis ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00599 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00599 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623508 ID=BQ623508; Name=BQ623508; organism=Citrus sinensis; type=EST; length=649bp
GTTAAGTCTGTTGAGATGCACCACGAAGCTCTGCAGGAGGCCCTTCCGGG
TGACAATGTTGGCCTTCAACGTGAAGAACGTTGCTGTCAAGGATCTCAAG
CGTGGTTTTGTTGCTTCCAACTCGAAGGATGATCCGGCCAGGGAAGCTGC
CAACTTCACTTCACAGGTCATCATCATGAACCATCCTGGCCAGATTGGAA
ATGGATATGCCCCAGTGCTTGACTGCCACACCTCCCACATTGCCGTCAAG
TTTTCTGAGCTCGTGACGAAGATTGATAGGCGATCAGGCAAGGAGCTTGA
GAAGGAGCCCAAGTTCTTGAAGAATGGAGATGCCGGAATGGTTAAGATGA
TTCCCACCAAGCCCATGGTTGTTGAGACATTCTCCGACTACCCACCCCTT
GGTCGTTTTGCTGTTAGGGACATGCGTCAGACTGTTGCTGTGGGTGTTAT
CAAGAGTGTTGAGAAGAAGGATCCCACAGGTGCCAAGGTCACCAAGTCCG
CAGCCAAGAAGAAATGAGAAATTTTTATGGGTCTGTCTACAATAGTTAGG
GTTTATTTTCTGGCTTTTTTATTTTCTTTTCTGGTTTTGGTTTGCATCAA
TGTTTCTGCAAACAGCCCTATTTGCTTCCACCAGTTTGCTGGTGAGCAT
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