BQ623525

Overview
NameBQ623525
Unique NameBQ623525
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length695
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2449 contig Csv1_Contig2449:480..1174. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA4_PHAVU (Glutamine synthetase leaf isozyme, chloroplastic OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 1.362e-83
Identity = 107/157 (68.15%), Postives = 122/157 (77.71%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RH AA++FS+  V+AE PW+GIEQEYTLLQ +V WPL                   ADK++GRDI D+HYKACL+AGINISG NGEVMPGQWE+QVGP+VGI AGD +W +RYILERITE AGVVLS DPKPI+GDWNGAG H NYSTKSMR DGGF
Sbjct:  164 RHRAAEVFSNPRVIAEVPWFGIEQEYTLLQTNVNWPLGWPVGGYPGPQGPYYCSAGADKSFGRDISDAHYKACLFAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWASRYILERITEQAGVVLSLDPKPIEGDWNGAGCHTNYSTKSMREDGGF 320

HSP 2 Score: 100.908 bits (250), Expect = 1.362e-83
Identity = 50/61 (81.97%), Postives = 52/61 (85.25%), Query Frame = 3 
Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEK 653
            FE IKKAI  L LRH EHI+AYGEGNERRLTGKHETA INTF+ GVANRG SIRVG DTEK
Sbjct:  320 FEVIKKAILNLSLRHKEHISAYGEGNERRLTGKHETASINTFSWGVANRGCSIRVGRDTEK 380
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA2_DAUCA (Glutamine synthetase, chloroplastic OS=Daucus carota GN=GLN2 PE=2 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 1.941e-82
Identity = 107/157 (68.15%), Postives = 121/157 (77.07%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RH AA+IFS + V+ E PW+GIEQEYTL+Q+DV WPL                   ADK++GRDI D+HYKACLYAGINISG NGEVMPGQWEFQVGP+VGI AGD +W ARY+LERITE AGVVL+ DPKPI GDWNGAG H NYSTKSMR +GGF
Sbjct:  167 RHKAAQIFSDAKVLGEVPWFGIEQEYTLMQQDVNWPLGWNVGGYPGPQGPYYCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEFQVGPSVGIEAGDHIWCARYLLERITEQAGVVLTLDPKPIDGDWNGAGCHTNYSTKSMREEGGF 323

HSP 2 Score: 97.4413 bits (241), Expect = 1.941e-82
Identity = 48/62 (77.42%), Postives = 53/62 (85.48%), Query Frame = 3 
Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEKE 656
            FE IKKAI  L LRH EHI+AYGEGNERRLTGKHETA I++F+ GVA+RG SIRVG DTEKE
Sbjct:  323 FEVIKKAILNLSLRHKEHISAYGEGNERRLTGKHETASIDSFSWGVADRGCSIRVGRDTEKE 384
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA2_PEA (Glutamine synthetase leaf isozyme, chloroplastic OS=Pisum sativum GN=GS2 PE=2 SV=2)

HSP 1 Score: 231.876 bits (590), Expect = 3.303e-82
Identity = 109/157 (69.43%), Postives = 122/157 (77.71%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RH AA+IFS+  V AE PWYGIEQEYTLLQ +VKWPL                   ADK++GRDI D+HYKAC+YAGINISG NGEVMPGQWE+QVGP+VGI AGD +W +RYILERITE AGVVL+ DPKPI+GDWNGAG H NYSTKSMR DGGF
Sbjct:  165 RHRAAEIFSNPKVEAEIPWYGIEQEYTLLQTNVKWPLGWPVGGYPGPQGPYYCAAGADKSFGRDISDAHYKACIYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWASRYILERITEQAGVVLTLDPKPIEGDWNGAGCHTNYSTKSMREDGGF 321

HSP 2 Score: 94.7449 bits (234), Expect = 3.303e-82
Identity = 48/61 (78.69%), Postives = 49/61 (80.33%), Query Frame = 3 
Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEK 653
            FE IKKAI  L LRH  HI AYGEGNERRLTGKHETA IN F+ GVANRG SIRVG DTEK
Sbjct:  321 FEVIKKAILNLSLRHKIHIEAYGEGNERRLTGKHETASINDFSWGVANRGCSIRVGRDTEK 381
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA2_ARATH (Glutamine synthetase, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=GLN2 PE=1 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 1.627e-81
Identity = 108/157 (68.79%), Postives = 120/157 (76.43%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            R  AA+IFS+  V  E PW+GIEQEYTLLQ++VKWPL                   ADK WGRDI D+HYKACLYAGINISG NGEVMPGQWEFQVGP+VGI AGD +W ARY+LERITE AGVVL+ DPKPI+GDWNGAG H NYSTKSMR +GGF
Sbjct:  165 RAKAAEIFSNKKVSGEVPWFGIEQEYTLLQQNVKWPLGWPVGAFPGPQGPYYCGVGADKIWGRDISDAHYKACLYAGINISGTNGEVMPGQWEFQVGPSVGIDAGDHVWCARYLLERITEQAGVVLTLDPKPIEGDWNGAGCHTNYSTKSMREEGGF 321

HSP 2 Score: 94.7449 bits (234), Expect = 1.627e-81
Identity = 47/60 (78.33%), Postives = 50/60 (83.33%), Query Frame = 3 
Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTE 650
            FE IKKAI  L LRH EHI+AYGEGNERRLTGKHETA I+ F+ GVANRG SIRVG DTE
Sbjct:  321 FEVIKKAILNLSLRHKEHISAYGEGNERRLTGKHETASIDQFSWGVANRGCSIRVGRDTE 380
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNAC_MAIZE (Glutamine synthetase, chloroplastic OS=Zea mays GN=GLN2 PE=2 SV=1)

HSP 1 Score: 230.72 bits (587), Expect = 2.768e-81
Identity = 108/157 (68.79%), Postives = 121/157 (77.07%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RH AA+IFS   V  + PW+GIEQEYTLLQKDV WPL                   ADK++GRDI D+HYKACLYAGINISG NGEVMPGQWE+QVGP+VGI AGD +W++RYILERITE AGVVL+ DPKPIQGDWNGAG H NYSTK+MR DGGF
Sbjct:  158 RHRAAQIFSDPKVGEQVPWFGIEQEYTLLQKDVNWPLGWPVGGFPGPQGPYYCAVGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWISRYILERITEQAGVVLTLDPKPIQGDWNGAGCHTNYSTKTMREDGGF 314

HSP 2 Score: 92.8189 bits (229), Expect = 2.768e-81
Identity = 46/60 (76.67%), Postives = 49/60 (81.67%), Query Frame = 3 
Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTE 650
            FE IK+AI  L LRH  HI+AYGEGNERRLTGKHETA I TF+ GVANRG SIRVG DTE
Sbjct:  314 FEEIKRAILNLSLRHDLHISAYGEGNERRLTGKHETASIGTFSWGVANRGCSIRVGRDTE 373
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNAC_BRANA (Glutamine synthetase, chloroplastic OS=Brassica napus GN=GLN2 PE=2 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 3.610e-81
Identity = 107/157 (68.15%), Postives = 119/157 (75.80%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            R  AA+IFS+  V  E PW+GIEQEYTLLQ +V WPL                   A+K+WGRDI D+HYKACLYAGINISG NGEVMPGQWEFQVGP+VGI AGD +W ARY+LERITE AGVVL+ DPKPI+GDWNGAG H NYSTKSMR DGGF
Sbjct:  163 RARAAEIFSNKKVNEEIPWFGIEQEYTLLQPNVNWPLGWPVGAYPGPQGPYYCGVGAEKSWGRDISDAHYKACLYAGINISGTNGEVMPGQWEFQVGPSVGIEAGDHVWCARYLLERITEQAGVVLTLDPKPIEGDWNGAGCHTNYSTKSMREDGGF 319

HSP 2 Score: 96.6709 bits (239), Expect = 3.610e-81
Identity = 48/62 (77.42%), Postives = 52/62 (83.87%), Query Frame = 3 
Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEKE 656
            FE IKKAI  L LRH EHI+AYGEGNERRLTGKHETA I+ F+ GVANRG SIRVG DTEK+
Sbjct:  319 FEVIKKAILNLSLRHMEHISAYGEGNERRLTGKHETASIDQFSWGVANRGCSIRVGRDTEKK 380
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA2_ORYSJ (Glutamine synthetase, chloroplastic OS=Oryza sativa subsp. japonica GN=GLN2 PE=1 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 5.145e-80
Identity = 106/157 (67.52%), Postives = 123/157 (78.34%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            R+ AA++FS   VV++ PW+GIEQEYTLLQ+DV WPL                   +DK++GRDI D+HYKACLYAGINISG NGEVMPGQWE+QVGP+VGI AGD +W++RYILERITE AGVVL+ DPKPIQGDWNGAG H NYSTKSMR DGGF
Sbjct:  163 RNRAAQVFSDPKVVSQVPWFGIEQEYTLLQRDVNWPLGWPVGGYPGPQGPYYCAVGSDKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWISRYILERITEQAGVVLTLDPKPIQGDWNGAGCHTNYSTKSMREDGGF 319

HSP 2 Score: 89.3521 bits (220), Expect = 5.145e-80
Identity = 45/60 (75.00%), Postives = 48/60 (80.00%), Query Frame = 3 
Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTE 650
            FE IKKAI  L LRH  HI+AYGEGNERRLTG HETA I+ F+ GVANRG SIRVG DTE
Sbjct:  319 FEVIKKAILNLSLRHDLHISAYGEGNERRLTGLHETASIDNFSWGVANRGCSIRVGRDTE 378
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA2_HORVU (Glutamine synthetase leaf isozyme, chloroplastic OS=Hordeum vulgare PE=2 SV=2)

HSP 1 Score: 224.557 bits (571), Expect = 2.767e-78
Identity = 104/157 (66.24%), Postives = 119/157 (75.80%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RH AA+IFS   V ++ PW+GIEQEYTL+Q+DV WPL                   +DK++GRDI D+HYKACLYAGI ISG NGEVMPGQWE+QVGP+VGI AGD +W +RYILERITE AGVVL+ DPKPIQGDWNGAG H NYST SMR DGGF
Sbjct:  169 RHMAAQIFSDPKVTSQVPWFGIEQEYTLMQRDVNWPLGWPVGGYPGPQGPYYCAVGSDKSFGRDISDAHYKACLYAGIEISGTNGEVMPGQWEYQVGPSVGIDAGDHIWASRYILERITEQAGVVLTLDPKPIQGDWNGAGCHTNYSTLSMREDGGF 325

HSP 2 Score: 88.9669 bits (219), Expect = 2.767e-78
Identity = 45/60 (75.00%), Postives = 48/60 (80.00%), Query Frame = 3 
Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTE 650
            F+ IKKAI  L LRH  HIAAYGEGNERRLTG HETA I+ F+ GVANRG SIRVG DTE
Sbjct:  325 FDVIKKAILNLSLRHDLHIAAYGEGNERRLTGLHETASISDFSWGVANRGCSIRVGRDTE 384
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA_VIGAC (Glutamine synthetase nodule isozyme OS=Vigna aconitifolia PE=2 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 1.102e-71
Identity = 148/235 (62.98%), Postives = 160/235 (68.09%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF*GY*ESN*ETWTEAQ*AHCRIW----*RQ*TPTHWQT*NS*HQHFQWGCC*PWGINPCWTRHRERRKGYFEDRRPAFN 694
            RH AAKIFSH DVVAEEPWYGIEQEYTLLQKDV WPL                   ADKA+GRDIVD+HYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGD+LWVARYILERITEIAGVVLSFDPKPI+GDWNGAGAH NYSTK+MRNDGG+    +S  E   +    H   +     R+ T  H     +    F WG               +  KGYFEDRRPA N
Sbjct:  107 RHNAAKIFSHPDVVAEEPWYGIEQEYTLLQKDVNWPLGWPVGGFPGPQGPYYCGAGADKAFGRDIVDAHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDELWVARYILERITEIAGVVLSFDPKPIKGDWNGAGAHTNYSTKTMRNDGGY-EVIKSAIEKLGKRHKEHIAAYGEGNERRLTGRHE---TADINTFLWGVANRGASIRVGRDTEKAGKGYFEDRRPASN 337
[Close]
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA1_ALNGL (Glutamine synthetase OS=Alnus glutinosa GN=GLN1 PE=2 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 2.714e-70
Identity = 144/235 (61.28%), Postives = 157/235 (66.81%), Query Frame = 2 
Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF*GY*ESN*ETWTEAQ*AHCRIW----*RQ*TPTHWQT*NS*HQHFQWGCC*PWGINPCWTRHRERRKGYFEDRRPAFN 694
            RH AAKIFSH +VVAE PWYGIEQEYTLLQKDVKWPL                   ADKAWGRDIVD+HYKACLYAGINISGINGEVMPGQWEFQVGP+VGISAGD++W ARYILERITEIAGVVLS DPKPIQGDWNGAGAH NYSTKSMRN+GG+    +   E        H   +     R+ T  H     +    F+WG               +  KGYFEDRRPA N
Sbjct:  107 RHGAAKIFSHPEVVAEVPWYGIEQEYTLLQKDVKWPLGWPVGGYPGPQGPYYCGIGADKAWGRDIVDAHYKACLYAGINISGINGEVMPGQWEFQVGPSVGISAGDEVWAARYILERITEIAGVVLSLDPKPIQGDWNGAGAHTNYSTKSMRNNGGY-EIIKKAIEKLGLRHKEHIAAYGEGNERRLTGRHE---TADINTFKWGVANRGASIRVGRDTEKEGKGYFEDRRPASN 337
The following BLAST results are available for this feature:
BLAST of BQ623525 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 88
Match NameE-valueIdentityDescription
GLNA4_PHAVU1.362e-8368.15Glutamine synthetase leaf isozyme, chloroplastic O... [more]
GLNA2_DAUCA1.941e-8268.15Glutamine synthetase, chloroplastic OS=Daucus caro... [more]
GLNA2_PEA3.303e-8269.43Glutamine synthetase leaf isozyme, chloroplastic O... [more]
GLNA2_ARATH1.627e-8168.79Glutamine synthetase, chloroplastic/mitochondrial ... [more]
GLNAC_MAIZE2.768e-8168.79Glutamine synthetase, chloroplastic OS=Zea mays GN... [more]
GLNAC_BRANA3.610e-8168.15Glutamine synthetase, chloroplastic OS=Brassica na... [more]
GLNA2_ORYSJ5.145e-8067.52Glutamine synthetase, chloroplastic OS=Oryza sativ... [more]
GLNA2_HORVU2.767e-7866.24Glutamine synthetase leaf isozyme, chloroplastic O... [more]
GLNA_VIGAC1.102e-7162.98Glutamine synthetase nodule isozyme OS=Vigna aconi... [more]
GLNA1_ALNGL2.714e-7061.28Glutamine synthetase OS=Alnus glutinosa GN=GLN1 PE... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUSDA-FP_00616 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00616 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623525 ID=BQ623525; Name=BQ623525; organism=Citrus sinensis; type=EST; length=695bp
AAGACATGCAGCTGCCAAGATTTTCAGCCATTCTGACGTTGTGGCTGAAG
AACCATGGTATGGTATTGAGCAGGAATACACCTTACTGCAGAAGGATGTT
AAATGGCCTCTTGGCTGGCCCATCGGTGGTTATCCTGGGCCTCAGGGACC
ATACTACTGTGGTGTTGGTGCTGACAAAGCTTGGGGGCGCGACATTGTAG
ACTCCCATTACAAGGCCTGTCTATATGCAGGAATCAACATCAGTGGCATC
AATGGAGAAGTCATGCCGGGGCAGTGGGAGTTCCAAGTTGGTCCCGCTGT
TGGAATTTCTGCTGGAGATCAGTTGTGGGTTGCCCGCTATATTTTAGAGA
GGATTACAGAGATTGCTGGAGTTGTGCTTTCTTTCGATCCCAAGCCAATT
CAGGGTGACTGGAATGGAGCTGGTGCTCACGCAAATTACAGCACCAAGTC
GATGAGGAACGATGGAGGCTTTTGAGGGTATTAAGAAAGCAATTGAGAAA
CTTGGACTGAGGCACAGTGAGCACATTGCCGCATATGGTGAAGGCAATGA
ACGCCGACTCACTGGCAAACATGAAACAGCTGACATCAACACTTTCAATG
GGGGTGTTGCTAACCGTGGGGCATCAATCCGTGTTGGACCAGACACAGAG
AAAGAAGGAAGGGGTACTTTGAGGACAGGAGACCTGCTTTTAACA
back to top