C21854

Overview
NameC21854
Unique NameC21854
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length282
Libraries
Library NameType
Valencia orange young seeds cDNAcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RHOA_CANFA (Transforming protein RhoA OS=Canis familiaris GN=RHOA PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.195e-18
Identity = 40/76 (52.63%), Postives = 53/76 (69.74%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            + C+LI ++ ++FP  Y+PTVF+N+ A+  VDG  V L LWDTAGQEDY RLRPLSY   D+  + FS+ S  S E
Sbjct:   18 KTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLE 93
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RHOA_BOVIN (Transforming protein RhoA OS=Bos taurus GN=RHOA PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.195e-18
Identity = 40/76 (52.63%), Postives = 53/76 (69.74%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            + C+LI ++ ++FP  Y+PTVF+N+ A+  VDG  V L LWDTAGQEDY RLRPLSY   D+  + FS+ S  S E
Sbjct:   18 KTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLE 93
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RAC2_CAEEL (Ras-related protein rac-2 OS=Caenorhabditis elegans GN=rac-2 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.195e-18
Identity = 39/76 (51.32%), Postives = 54/76 (71.05%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            + C+L+ YT+N FP +YI TVFD +S N  VDG  +NL LWDTAGQ+DY + R LS+   D+F + F+L + AS+E
Sbjct:   16 KTCLLLSYTTNAFPGEYILTVFDTYSTNVMVDGRPINLSLWDTAGQDDYDQFRHLSFPQTDVFLVCFALNNPASFE 91
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RHOB_RAT (Rho-related GTP-binding protein RhoB OS=Rattus norvegicus GN=Rhob PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 9.244e-18
Identity = 40/76 (52.63%), Postives = 53/76 (69.74%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            + C+LI ++ ++FP  Y+PTVF+N+ A+  VDG  V L LWDTAGQEDY RLRPLSY   D+  + FS+ S  S E
Sbjct:   18 KTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLE 93

HSP 2 Score: 21.9422 bits (45), Expect = 9.244e-18
Identity = 6/13 (46.15%), Postives = 11/13 (84.62%), Query Frame = 1 
Query:  232 NVLKKWMPELRRF 270
            N+ +KW+PE++ F
Sbjct:   94 NIPEKWVPEVKHF 106
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RHOB_MOUSE (Rho-related GTP-binding protein RhoB OS=Mus musculus GN=Rhob PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 9.244e-18
Identity = 40/76 (52.63%), Postives = 53/76 (69.74%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            + C+LI ++ ++FP  Y+PTVF+N+ A+  VDG  V L LWDTAGQEDY RLRPLSY   D+  + FS+ S  S E
Sbjct:   18 KTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLE 93

HSP 2 Score: 21.9422 bits (45), Expect = 9.244e-18
Identity = 6/13 (46.15%), Postives = 11/13 (84.62%), Query Frame = 1 
Query:  232 NVLKKWMPELRRF 270
            N+ +KW+PE++ F
Sbjct:   94 NIPEKWVPEVKHF 106
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RHOB_HUMAN (Rho-related GTP-binding protein RhoB OS=Homo sapiens GN=RHOB PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 9.244e-18
Identity = 40/76 (52.63%), Postives = 53/76 (69.74%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            + C+LI ++ ++FP  Y+PTVF+N+ A+  VDG  V L LWDTAGQEDY RLRPLSY   D+  + FS+ S  S E
Sbjct:   18 KTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLE 93

HSP 2 Score: 21.9422 bits (45), Expect = 9.244e-18
Identity = 6/13 (46.15%), Postives = 11/13 (84.62%), Query Frame = 1 
Query:  232 NVLKKWMPELRRF 270
            N+ +KW+PE++ F
Sbjct:   94 NIPEKWVPEVKHF 106
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RHOB_BOVIN (Rho-related GTP-binding protein RhoB OS=Bos taurus GN=RHOB PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 9.244e-18
Identity = 40/76 (52.63%), Postives = 53/76 (69.74%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            + C+LI ++ ++FP  Y+PTVF+N+ A+  VDG  V L LWDTAGQEDY RLRPLSY   D+  + FS+ S  S E
Sbjct:   18 KTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLE 93

HSP 2 Score: 21.9422 bits (45), Expect = 9.244e-18
Identity = 6/13 (46.15%), Postives = 11/13 (84.62%), Query Frame = 1 
Query:  232 NVLKKWMPELRRF 270
            N+ +KW+PE++ F
Sbjct:   94 NIPEKWVPEVKHF 106
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RHO1_DROME (Ras-like GTP-binding protein Rho1 OS=Drosophila melanogaster GN=Rho1 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.070e-17
Identity = 40/76 (52.63%), Postives = 53/76 (69.74%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            + C+LI ++ ++FP  Y+PTVF+N+ A+  VDG  V L LWDTAGQEDY RLRPLSY   D+  + FS+ S  S E
Sbjct:   18 KTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLE 93
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RHOU_HUMAN (Rho-related GTP-binding protein RhoU OS=Homo sapiens GN=RHOU PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.191e-17
Identity = 39/76 (51.32%), Postives = 56/76 (73.68%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            +  +++ YT+N +PT+YIPT FDNFSA  +VDG  V L L DTAGQ+++ +LRPL Y   DIF L FS++S +S++
Sbjct:   62 KTSLVVSYTTNGYPTEYIPTAFDNFSAVVSVDGRPVRLQLCDTAGQDEFDKLRPLCYTNTDIFLLCFSVVSPSSFQ 137

HSP 2 Score: 21.9422 bits (45), Expect = 1.191e-17
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  232 NVLKKWMPELR 264
            NV +KW+PE+R
Sbjct:  138 NVSEKWVPEIR 148
[Close]
BLAST of C21854 vs. ExPASy Swiss-Prot
Match: RHOU_BOVIN (Rho-related GTP-binding protein RhoU OS=Bos taurus GN=RHOU PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.191e-17
Identity = 39/76 (51.32%), Postives = 56/76 (73.68%), Query Frame = 2 
Query:    5 RACMLIXYTSNKFPTDYIPTVFDNFSANXAVDGSIVNLGLWDTAGQEDYSRLRPLSYRGADIFXLAFSLISRASYE 232
            +  +++ YT+N +PT+YIPT FDNFSA  +VDG  V L L DTAGQ+++ +LRPL Y  ADIF L FS++  +S++
Sbjct:   59 KTSLVVSYTTNGYPTEYIPTAFDNFSAVVSVDGRPVKLQLCDTAGQDEFDKLRPLCYTNADIFLLCFSVVGPSSFQ 134

HSP 2 Score: 21.9422 bits (45), Expect = 1.191e-17
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  232 NVLKKWMPELR 264
            NV +KW+PE+R
Sbjct:  135 NVSEKWVPEIR 145
The following BLAST results are available for this feature:
BLAST of C21854 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 138
Match NameE-valueIdentityDescription
RHOA_CANFA8.195e-1852.63Transforming protein RhoA OS=Canis familiaris GN=R... [more]
RHOA_BOVIN8.195e-1852.63Transforming protein RhoA OS=Bos taurus GN=RHOA PE... [more]
RAC2_CAEEL8.195e-1851.32Ras-related protein rac-2 OS=Caenorhabditis elegan... [more]
RHOB_RAT9.244e-1852.63Rho-related GTP-binding protein RhoB OS=Rattus nor... [more]
RHOB_MOUSE9.244e-1852.63Rho-related GTP-binding protein RhoB OS=Mus muscul... [more]
RHOB_HUMAN9.244e-1852.63Rho-related GTP-binding protein RhoB OS=Homo sapie... [more]
RHOB_BOVIN9.244e-1852.63Rho-related GTP-binding protein RhoB OS=Bos taurus... [more]
RHO1_DROME1.070e-1752.63Ras-like GTP-binding protein Rho1 OS=Drosophila me... [more]
RHOU_HUMAN1.191e-1751.32Rho-related GTP-binding protein RhoU OS=Homo sapie... [more]
RHOU_BOVIN1.191e-1751.32Rho-related GTP-binding protein RhoU OS=Bos taurus... [more]

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Properties
Property NameValue
Genbank descriptionC21854 Valencia orange young seeds cDNA Citrus sinensis cDNA clone corVSSN-28R 3, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>C21854 ID=C21854; Name=C21854; organism=Citrus sinensis; type=EST; length=282bp
GGCACGAGCATGTATGCTCATTTNTTACACAAGTAACAAGTTCCCCACTG
ATTACATACCCACAGTGTTTGATAATTTCAGTGCCAATNTTGCTGTTGAT
GGTAGTATTGTGAACCTGGGCTTATGGGATACTGCAGGGCAGGAAGATTA
TAGCAGGTTGAGGCCACTGAGTTACAGAGGTGCAGACATTTTTNTNCTGG
CTTTCTCATTAATTAGTAGGGCAAGTTATGAAACGTTCTTAAGAAGTGGA
TGCCTGAACTTCGACGNTTCGACCAATGTACC
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