CB250245

Overview
NameCB250245
Unique NameCB250245
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length709
Libraries
Library NameType
Mature fruit abscission zone cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13846 contig Csv1_Contig13846:683..1391. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP70_DAUCA (Heat shock 70 kDa protein OS=Daucus carota GN=HSP70 PE=3 SV=1)

HSP 1 Score: 347.051 bits (889), Expect = 5.615e-95
Identity = 182/240 (75.83%), Postives = 194/240 (80.83%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSS-TAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDN----NLLGKFDPSGIPPAPRG 707
            R+VN FV EF   NKKDI     ALRRLRTACERAKRTLSS TAQTTIEIDSLYEG+DFYTTITRARFEELNMDLF+KCM+PVEKCLRD+K+DK+ VH+VVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDE           ILSGEGNE+  D             ETAGGVMTVLIPRNTTIPTK EQ+FSTYSDNQPGVLIQVYEGER RTRDN     LLGKF+ +GIPPAPRG
Sbjct:  240 RLVNRFVTEFLTNNKKDIRWECEALRRLRTACERAKRTLSSSTAQTTIEIDSLYEGVDFYTTITRARFEELNMDLFKKCMDPVEKCLRDSKIDKAQVHEVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNER-SDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTRDNKLLGKLLGKFELTGIPPAPRG 478
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_SAGOE (Heat shock cognate 71 kDa protein OS=Saguinus oedipus GN=HSPA8 PE=2 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 3.081e-93
Identity = 172/235 (73.19%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_RAT (Heat shock cognate 71 kDa protein OS=Rattus norvegicus GN=Hspa8 PE=1 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 3.081e-93
Identity = 172/235 (73.19%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_PONAB (Heat shock cognate 71 kDa protein OS=Pongo abelii GN=HSPA8 PE=2 SV=2)

HSP 1 Score: 341.273 bits (874), Expect = 3.081e-93
Identity = 172/235 (73.19%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_MOUSE (Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 3.081e-93
Identity = 172/235 (73.19%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_HUMAN (Heat shock cognate 71 kDa protein OS=Homo sapiens GN=HSPA8 PE=1 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 3.081e-93
Identity = 172/235 (73.19%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_HORSE (Heat shock cognate 71 kDa protein OS=Equus caballus GN=HSPA8 PE=2 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 3.081e-93
Identity = 172/235 (73.19%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_BOVIN (Heat shock cognate 71 kDa protein OS=Bos taurus GN=HSPA8 PE=1 SV=2)

HSP 1 Score: 341.273 bits (874), Expect = 3.081e-93
Identity = 172/235 (73.19%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_DANRE (Heat shock cognate 71 kDa protein OS=Danio rerio GN=hspa8 PE=2 SV=1)

HSP 1 Score: 340.502 bits (872), Expect = 5.255e-93
Identity = 171/235 (72.77%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAKMDK+ +HD+VLVGGSTRIPK+Q+LLQD+FNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFITEFKRKHKKDISDNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKMDKAQIHDIVLVGGSTRIPKIQKLLQDYFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP70_ONCMY (Heat shock cognate 70 kDa protein OS=Oncorhynchus mykiss GN=hsc71 PE=2 SV=2)

HSP 1 Score: 340.502 bits (872), Expect = 5.255e-93
Identity = 173/235 (73.62%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAKMDK+ VHD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRKYKKDISDNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKSLRDAKMDKAQVHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
The following BLAST results are available for this feature:
BLAST of CB250245 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HSP70_DAUCA5.615e-9575.83Heat shock 70 kDa protein OS=Daucus carota GN=HSP7... [more]
HSP7C_SAGOE3.081e-9373.19Heat shock cognate 71 kDa protein OS=Saguinus oedi... [more]
HSP7C_RAT3.081e-9373.19Heat shock cognate 71 kDa protein OS=Rattus norveg... [more]
HSP7C_PONAB3.081e-9373.19Heat shock cognate 71 kDa protein OS=Pongo abelii ... [more]
HSP7C_MOUSE3.081e-9373.19Heat shock cognate 71 kDa protein OS=Mus musculus ... [more]
HSP7C_HUMAN3.081e-9373.19Heat shock cognate 71 kDa protein OS=Homo sapiens ... [more]
HSP7C_HORSE3.081e-9373.19Heat shock cognate 71 kDa protein OS=Equus caballu... [more]
HSP7C_BOVIN3.081e-9373.19Heat shock cognate 71 kDa protein OS=Bos taurus GN... [more]
HSP7C_DANRE5.255e-9372.77Heat shock cognate 71 kDa protein OS=Danio rerio G... [more]
HSP70_ONCMY5.255e-9373.62Heat shock cognate 70 kDa protein OS=Oncorhynchus ... [more]

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Properties
Property NameValue
Genbank descriptionEST0007 Mature fruit abscission zone cDNA subtraction library Citrus sinensis cDNA clone MFAZ007 similar to 70 kDa heat shock protein (acc# P27322), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB250245 ID=CB250245; Name=CB250245; organism=Citrus sinensis; type=EST; length=709bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACCGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACTGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCCTGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGG
TTCTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAG
GACTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGC
GGTTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTA
ATGAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTG
GGTTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACAC
CACTATTCCAACCAAGAATGAGCAGGTGTTCTCCACATACTCTGACAACC
AGCCCGGGGTGTTGATCCAGGTCTACGAGGGTGAGCGCACAAGAACCCGT
GACAACAACTTGCTTGGAAAGTTTGATCCCTCTGGCATTCCTCCTGCTCC
AAGAGGGGT
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