CB250245

Overview
NameCB250245
Unique NameCB250245
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length709
Libraries
Library NameType
Mature fruit abscission zone cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: BIP_SPIOL (Luminal-binding protein OS=Spinacia oleracea GN=HSC70 PE=2 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 9.027e-69
Identity = 132/235 (56.17%), Postives = 166/235 (70.64%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ +F++  K+K+ KDIS + RAL +LR  CERAKR LSS  Q  +EI+SL++G+DF   +TRARFEELN DLFRK M PV+K + DA ++K+ + ++VLVGGSTRIPKVQQLL++FFNGKE  K +NPDE           ILSGEG E+ ++             ET GGVMT LIPRNT IPTK  QVF+TY D Q  V IQV+EGER+ T+D  LLGKFD +GI PAPRG
Sbjct:  268 RLMEYFIKLIKKKHTKDISKDNRALGKLRRECERAKRALSSQHQVRVEIESLFDGVDFSEPLTRARFEELNNDLFRKTMGPVKKAMDDAGLEKNQIDEIVLVGGSTRIPKVQQLLKEFFNGKEPSKGVNPDEAVAFGAAVQGSILSGEGGEETKEILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVTIQVFEGERSLTKDCRLLGKFDLTGIAPAPRG 502
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS7C4_DICDI (Heat shock cognate 70 kDa protein 4 OS=Dictyostelium discoideum GN=DDB_G0293678 PE=3 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 2.011e-68
Identity = 130/235 (55.32%), Postives = 165/235 (70.21%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++NHF+ EFK K+KKDI+ N RA+ RLRTACERAKRTLS ++  +IEIDSLY+GIDFY+TITR  FE+L  DLF  C++ VE  L   K+DKS V ++VL GGST+IPK+Q++ +++FNGKE   SINPDE           ++SG+G+   Q              ET GG+MT L+ RNT IP K  Q FST  DNQ  VLIQ+YEGERT T+DNNL+G F+ +GIPPAPRG
Sbjct:  236 RLMNHFIDEFKLKHKKDITNNQRAIFRLRTACERAKRTLSISSLASIEIDSLYDGIDFYSTITRTHFEKLCSDLFHSCIDHVETILNKCKLDKSLVDEIVLAGGSTQIPKIQRMFKEYFNGKEFNNSINPDEVVAYGAAVQSGVISGKGSNFPQ-LIFLNVTPLSMGIETVGGIMTNLVERNTHIPIKKTQTFSTQYDNQTNVLIQIYEGERTMTKDNNLIGTFELNGIPPAPRG 469
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: BIP2_ARATH (Luminal-binding protein 2 OS=Arabidopsis thaliana GN=At5g42020 PE=1 SV=2)

HSP 1 Score: 258.455 bits (659), Expect = 2.626e-68
Identity = 131/235 (55.74%), Postives = 165/235 (70.21%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ +F++  K+K++KDIS + +AL +LR  CERAKR LSS  Q  +EI+SL++G+D    +TRARFEELN DLFRK M PV+K + DA + KS + ++VLVGGSTRIPKVQQLL+DFF GKE  K +NPDE           ILSGEG ++ +D             ET GGVMT LIPRNT IPTK  QVF+TY D Q  V IQV+EGER+ T+D  LLGKFD +G+PPAPRG
Sbjct:  267 RIMEYFIKLIKKKHQKDISKDNKALGKLRRECERAKRALSSQHQVRVEIESLFDGVDLSEPLTRARFEELNNDLFRKTMGPVKKAMDDAGLQKSQIDEIVLVGGSTRIPKVQQLLKDFFEGKEPNKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVSIQVFEGERSLTKDCRLLGKFDLTGVPPAPRG 501
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: BIP3_MAIZE (Luminal-binding protein 3 OS=Zea mays GN=BIPE3 PE=2 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 3.430e-68
Identity = 132/235 (56.17%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ +F++  K+K  KDIS + RAL +LR   ERAKR LS+  Q  +EI+SL++G DF   +TRARFEELN DLFRK M PV+K + DA ++KS +H++VLVGGSTRIPKVQQLL+D+FNGKE  K +NPDE           ILSGEG ++ +D             ET GGVMT LIPRNT IPTK  QVF+TY D Q  V IQV+EGER+ T+D  LLGKFD +GIP APRG
Sbjct:  265 RIMEYFIKLIKKKYSKDISKDNRALGKLRREAERAKRALSNQHQVRVEIESLFDGTDFSEPLTRARFEELNNDLFRKTMGPVKKAMEDAGLEKSQIHEIVLVGGSTRIPKVQQLLKDYFNGKEPNKGVNPDEAVAFGAAVQGSILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVSIQVFEGERSMTKDCRLLGKFDLNGIPSAPRG 499
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_ASPNG (78 kDa glucose-regulated protein homolog OS=Aspergillus niger GN=bipA PE=2 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 9.980e-68
Identity = 131/235 (55.74%), Postives = 168/235 (71.49%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+++HFV+ + +KN  D++ + +A+ +L+   E+AKRTLSS   T IEI++ + G DF  T+TRA+FEELNMDLF+K ++PVE+ L+DAK+ KS V D+VLVGGSTRIPKVQ LL++FF GK+  K INPDE           +LSGE  E   D             ET GGVMT LIPRNT IPT+  Q+FST +DNQP VLIQVYEGER+ T+DNNLLGKF+ +GIPPAPRG
Sbjct:  280 RVMDHFVKLYNKKNNVDVTKDLKAMGKLKREVEKAKRTLSSQMSTRIEIEAFHNGEDFSETLTRAKFEELNMDLFKKTLKPVEQVLKDAKVKKSEVDDIVLVGGSTRIPKVQALLEEFFGGKKASKGINPDEAVAFGAAVQGGVLSGE--EGTGDVVLMDVNPLTLGIETTGGVMTKLIPRNTVIPTRKSQIFSTAADNQPTVLIQVYEGERSLTKDNNLLGKFELTGIPPAPRG 512
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_ASPKA (78 kDa glucose-regulated protein homolog OS=Aspergillus kawachi GN=bipA PE=3 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 9.980e-68
Identity = 131/235 (55.74%), Postives = 168/235 (71.49%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+++HFV+ + +KN  D++ + +A+ +L+   E+AKRTLSS   T IEI++ + G DF  T+TRA+FEELNMDLF+K ++PVE+ L+DAK+ KS V D+VLVGGSTRIPKVQ LL++FF GK+  K INPDE           +LSGE  E   D             ET GGVMT LIPRNT IPT+  Q+FST +DNQP VLIQVYEGER+ T+DNNLLGKF+ +GIPPAPRG
Sbjct:  280 RVMDHFVKLYNKKNNVDVTKDLKAMGKLKREVEKAKRTLSSQMSTRIEIEAFHNGEDFSETLTRAKFEELNMDLFKKTLKPVEQVLKDAKVKKSEVDDIVLVGGSTRIPKVQALLEEFFGGKKASKGINPDEAVAFGAAVQGGVLSGE--EGTGDVVLMDVNPLTLGIETTGGVMTKLIPRNTVIPTRKSQIFSTAADNQPTVLIQVYEGERSLTKDNNLLGKFELTGIPPAPRG 512
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_ASPAW (78 kDa glucose-regulated protein homolog OS=Aspergillus awamori GN=bipA PE=2 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 9.980e-68
Identity = 131/235 (55.74%), Postives = 168/235 (71.49%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+++HFV+ + +KN  D++ + +A+ +L+   E+AKRTLSS   T IEI++ + G DF  T+TRA+FEELNMDLF+K ++PVE+ L+DAK+ KS V D+VLVGGSTRIPKVQ LL++FF GK+  K INPDE           +LSGE  E   D             ET GGVMT LIPRNT IPT+  Q+FST +DNQP VLIQVYEGER+ T+DNNLLGKF+ +GIPPAPRG
Sbjct:  280 RVMDHFVKLYNKKNNVDVTKDLKAMGKLKREVEKAKRTLSSQMSTRIEIEAFHNGEDFSETLTRAKFEELNMDLFKKTLKPVEQVLKDAKVKKSEVDDIVLVGGSTRIPKVQALLEEFFGGKKASKGINPDEAVAFGAAVQGGVLSGE--EGTGDVVLMDVNPLTLGIETTGGVMTKLIPRNTVIPTRKSQIFSTAADNQPTVLIQVYEGERSLTKDNNLLGKFELTGIPPAPRG 512
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: BIP2_MAIZE (Luminal-binding protein 2 OS=Zea mays GN=BIPE2 PE=1 SV=3)

HSP 1 Score: 256.144 bits (653), Expect = 1.303e-67
Identity = 131/235 (55.74%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ +F++  K+K  KDIS + RAL +LR   ERAKR LS+  Q  +EI+SL++G DF   +TRARFEELN DLFRK M PV+K + DA ++KS +H++VLVGGSTRIPKVQQLL+D+F+GKE  K +NPDE           ILSGEG ++ +D             ET GGVMT LIPRNT IPTK  QVF+TY D Q  V IQV+EGER+ T+D  LLGKFD +GI PAPRG
Sbjct:  265 RIMEYFIKLIKKKYSKDISKDNRALGKLRREAERAKRALSNQHQVRVEIESLFDGTDFSEPLTRARFEELNNDLFRKTMGPVKKAMEDAGLEKSQIHEIVLVGGSTRIPKVQQLLRDYFDGKEPNKGVNPDEAVAFGAAVQGSILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVSIQVFEGERSMTKDCRLLGKFDLNGIAPAPRG 499
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_DROME (Heat shock 70 kDa protein cognate 3 OS=Drosophila melanogaster GN=Hsc70-3 PE=2 SV=2)

HSP 1 Score: 254.603 bits (649), Expect = 3.793e-67
Identity = 132/235 (56.17%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+++HF++ +K+K  KDI  + RA+++LR   E+AKR LS + Q  IEI+S +EG DF  T+TRA+FEELN+DLFR  ++PV+K L DA M+K  VH++VLVGGSTRIPKVQQL++DFF GKE  + INPDE           +LSGE  +                 ET GGVMT LIPRNT IPTK  QVFST SDNQ  V IQVYEGER  T+DN+LLGKFD +GIPPAPRG
Sbjct:  261 RVMDHFIKLYKKKKGKDIRKDNRAVQKLRREVEKAKRALSGSHQVRIEIESFFEGDDFSETLTRAKFEELNLDLFRSTLKPVQKVLEDADMNKKDVHEIVLVGGSTRIPKVQQLVKDFFGGKEPSRGINPDEAVAYGAAVQAGVLSGE--QDTDAIVLLDVNPLTMGIETVGGVMTKLIPRNTVIPTKKSQVFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRG 493
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: BIP4_TOBAC (Luminal-binding protein 4 OS=Nicotiana tabacum GN=BIP4 PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 6.469e-67
Identity = 130/235 (55.32%), Postives = 165/235 (70.21%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ +F++  K+K+ KDIS + RAL +LR   ERAKR LSS  Q  +EI+SL++G+DF   +TRARFEELN DLFRK M PV+K + DA ++K+ + ++VLVGGSTRIPKVQQLL+D+F+GKE  K +NPDE           ILSGEG ++ +D             ET GGVMT LIPRNT IPTK  QVF+TY D Q  V IQV+EGER+ T+D  LLGKFD +GI PAPRG
Sbjct:  269 RIMEYFIKLIKKKHGKDISKDNRALGKLRREAERAKRALSSQHQVRVEIESLFDGVDFSEPLTRARFEELNNDLFRKTMGPVKKAMDDAGLEKTQIDEIVLVGGSTRIPKVQQLLKDYFDGKEPNKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVTIQVFEGERSLTKDCRLLGKFDLTGIAPAPRG 503
The following BLAST results are available for this feature:
BLAST of CB250245 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
BIP_SPIOL9.027e-6956.17Luminal-binding protein OS=Spinacia oleracea GN=HS... [more]
HS7C4_DICDI2.011e-6855.32Heat shock cognate 70 kDa protein 4 OS=Dictyosteli... [more]
BIP2_ARATH2.626e-6855.74Luminal-binding protein 2 OS=Arabidopsis thaliana ... [more]
BIP3_MAIZE3.430e-6856.17Luminal-binding protein 3 OS=Zea mays GN=BIPE3 PE=... [more]
GRP78_ASPNG9.980e-6855.7478 kDa glucose-regulated protein homolog OS=Asperg... [more]
GRP78_ASPKA9.980e-6855.7478 kDa glucose-regulated protein homolog OS=Asperg... [more]
GRP78_ASPAW9.980e-6855.7478 kDa glucose-regulated protein homolog OS=Asperg... [more]
BIP2_MAIZE1.303e-6755.74Luminal-binding protein 2 OS=Zea mays GN=BIPE2 PE=... [more]
HSP7C_DROME3.793e-6756.17Heat shock 70 kDa protein cognate 3 OS=Drosophila ... [more]
BIP4_TOBAC6.469e-6755.32Luminal-binding protein 4 OS=Nicotiana tabacum GN=... [more]

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Properties
Property NameValue
Genbank descriptionEST0007 Mature fruit abscission zone cDNA subtraction library Citrus sinensis cDNA clone MFAZ007 similar to 70 kDa heat shock protein (acc# P27322), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB250245 ID=CB250245; Name=CB250245; organism=Citrus sinensis; type=EST; length=709bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACCGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACTGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCCTGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGG
TTCTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAG
GACTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGC
GGTTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTA
ATGAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTG
GGTTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACAC
CACTATTCCAACCAAGAATGAGCAGGTGTTCTCCACATACTCTGACAACC
AGCCCGGGGTGTTGATCCAGGTCTACGAGGGTGAGCGCACAAGAACCCGT
GACAACAACTTGCTTGGAAAGTTTGATCCCTCTGGCATTCCTCCTGCTCC
AAGAGGGGT
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