CB250245

Overview
NameCB250245
Unique NameCB250245
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length709
Libraries
Library NameType
Mature fruit abscission zone cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_PONAB (78 kDa glucose-regulated protein OS=Pongo abelii GN=HSPA5 PE=2 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 1.103e-66
Identity = 128/235 (54.47%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ HF++ +K+K  KD+  + RA+++LR   E+AKR LSS  Q  IEI+S YEG DF  T+TRA+FEELNMDLFR  M+PV+K L D+ + KS + ++VLVGGSTRIPK+QQL+++FFNGKE  + INPDE           +LSG+  +   D             ET GGVMT LIPRNT +PTK  Q+FST SDNQP V I+VYEGER  T+DN+LLG FD +GIPPAPRG
Sbjct:  261 RVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGD--QDTGDLALLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRG 493
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_HUMAN (78 kDa glucose-regulated protein OS=Homo sapiens GN=HSPA5 PE=1 SV=2)

HSP 1 Score: 253.062 bits (645), Expect = 1.103e-66
Identity = 128/235 (54.47%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ HF++ +K+K  KD+  + RA+++LR   E+AKR LSS  Q  IEI+S YEG DF  T+TRA+FEELNMDLFR  M+PV+K L D+ + KS + ++VLVGGSTRIPK+QQL+++FFNGKE  + INPDE           +LSG+  +   D             ET GGVMT LIPRNT +PTK  Q+FST SDNQP V I+VYEGER  T+DN+LLG FD +GIPPAPRG
Sbjct:  261 RVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGD--QDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRG 493
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_BOVIN (78 kDa glucose-regulated protein OS=Bos taurus GN=HSPA5 PE=2 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 1.103e-66
Identity = 128/235 (54.47%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ HF++ +K+K  KD+  + RA+++LR   E+AKR LSS  Q  IEI+S YEG DF  T+TRA+FEELNMDLFR  M+PV+K L D+ + KS + ++VLVGGSTRIPK+QQL+++FFNGKE  + INPDE           +LSG+  +   D             ET GGVMT LIPRNT +PTK  Q+FST SDNQP V I+VYEGER  T+DN+LLG FD +GIPPAPRG
Sbjct:  262 RVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGD--QDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRG 494
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: BIP5_TOBAC (Luminal-binding protein 5 OS=Nicotiana tabacum GN=BIP5 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.441e-66
Identity = 129/235 (54.89%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ +F++  K+K+ KDIS + RAL +LR   ERAKR LSS  Q  +EI+SL++G+DF   +TRARFEELN DLFRK M PV+K + DA ++K+ + ++VLVGGSTRIPKVQQLL+D+F+GKE  K +NPDE           ILSGEG ++ +D             ET GGVMT LIPRNT IPTK  QVF+TY D Q  V I V+EGER+ T+D  LLGKFD +GI PAPRG
Sbjct:  268 RIMEYFIKLIKKKHGKDISKDNRALGKLRREAERAKRALSSQHQVRVEIESLFDGVDFSEPLTRARFEELNNDLFRKTMGPVKKAMEDAGLEKNQIDEIVLVGGSTRIPKVQQLLKDYFDGKEPNKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVTISVFEGERSLTKDCRLLGKFDLTGIAPAPRG 502
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_RAT (78 kDa glucose-regulated protein OS=Rattus norvegicus GN=Hspa5 PE=1 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 3.211e-66
Identity = 127/235 (54.04%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ HF++ +K+K  KD+  + RA+++LR   E+AKR LSS  Q  IEI+S +EG DF  T+TRA+FEELNMDLFR  M+PV+K L D+ + KS + ++VLVGGSTRIPK+QQL+++FFNGKE  + INPDE           +LSG+  +   D             ET GGVMT LIPRNT +PTK  Q+FST SDNQP V I+VYEGER  T+DN+LLG FD +GIPPAPRG
Sbjct:  261 RVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGD--QDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRG 493
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_MOUSE (78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3)

HSP 1 Score: 251.521 bits (641), Expect = 3.211e-66
Identity = 127/235 (54.04%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ HF++ +K+K  KD+  + RA+++LR   E+AKR LSS  Q  IEI+S +EG DF  T+TRA+FEELNMDLFR  M+PV+K L D+ + KS + ++VLVGGSTRIPK+QQL+++FFNGKE  + INPDE           +LSG+  +   D             ET GGVMT LIPRNT +PTK  Q+FST SDNQP V I+VYEGER  T+DN+LLG FD +GIPPAPRG
Sbjct:  262 RVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGD--QDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRG 494
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_MESAU (78 kDa glucose-regulated protein OS=Mesocricetus auratus GN=HSPA5 PE=1 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 3.211e-66
Identity = 127/235 (54.04%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ HF++ +K+K  KD+  + RA+++LR   E+AKR LSS  Q  IEI+S +EG DF  T+TRA+FEELNMDLFR  M+PV+K L D+ + KS + ++VLVGGSTRIPK+QQL+++FFNGKE  + INPDE           +LSG+  +   D             ET GGVMT LIPRNT +PTK  Q+FST SDNQP V I+VYEGER  T+DN+LLG FD +GIPPAPRG
Sbjct:  261 RVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGD--QDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRG 493
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_CHICK (78 kDa glucose-regulated protein OS=Gallus gallus GN=HSPA5 PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 3.211e-66
Identity = 127/235 (54.04%), Postives = 164/235 (69.79%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ HF++ +K+K  KD+  + RA+++LR   E+AKR LSS  Q  IEI+S +EG DF  T+TRA+FEELNMDLFR  M+PV+K L D+ + KS + ++VLVGGSTRIPK+QQL+++FFNGKE  + INPDE           +LSG+  +   D             ET GGVMT LIPRNT +PTK  Q+FST SDNQP V I+VYEGER  T+DN+LLG FD +GIPPAPRG
Sbjct:  259 RVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGD--QDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRG 491
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: GRP78_CANGA (78 kDa glucose-regulated protein homolog OS=Candida glabrata GN=KAR2 PE=3 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 4.193e-66
Identity = 130/235 (55.32%), Postives = 161/235 (68.51%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            ++V   ++ FK+K+  D+S N +AL +L+   E+AKR LSS   T IEIDS  +GID   T+TRA+FEELN+DLF+K ++PVEK L DA ++K  + D VLVGGSTRIPKVQQLL+ FFNGK+  K INPDE           +LSGE  E V+D             ET GGVMT LI RNT IPTK  Q+FST  DNQP V I+VYEGER  ++DNNLLGKF+ +GIPPAPRG
Sbjct:  267 KIVRQLIKTFKKKHGIDVSDNEKALAKLKREAEKAKRALSSQMSTRIEIDSFVDGIDLSETLTRAKFEELNLDLFKKTLKPVEKVLEDAGLEKKDIDDFVLVGGSTRIPKVQQLLESFFNGKKASKGINPDEAVAYGAAVQAGVLSGE--EGVEDIVLLDVNALTLGIETTGGVMTPLIKRNTAIPTKKSQIFSTAVDNQPTVQIKVYEGERAMSKDNNLLGKFELTGIPPAPRG 499
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: BIP_SOLLC (Luminal-binding protein OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 4.193e-66
Identity = 129/235 (54.89%), Postives = 163/235 (69.36%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R++ +F++  K+K+ KDIS + RAL +LR   ERAKR+LSS  Q  +EI+SL++G DF   +TRARFEELN DLFRK M PV+K + DA + K+ + ++VLVGGSTRIPKVQQLL+D+F+GKE  K +NPDE           ILSGEG ++ +D             ET GGVMT  IPRNT IPTK  QVF+TY D Q  V IQV+EGER+ T+D   LGKFD +GIPPAPRG
Sbjct:  268 RIMEYFIKLIKKKHGKDISKDNRALGKLRREAERAKRSLSSQHQVRVEIESLFDGTDFSEPLTRARFEELNNDLFRKTMGPVKKAMDDAGLQKNQIDEIVLVGGSTRIPKVQQLLKDYFDGKEPSKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKFIPRNTVIPTKKSQVFTTYQDQQTTVSIQVFEGERSLTKDCRNLGKFDLTGIPPAPRG 502
The following BLAST results are available for this feature:
BLAST of CB250245 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GRP78_PONAB1.103e-6654.4778 kDa glucose-regulated protein OS=Pongo abelii G... [more]
GRP78_HUMAN1.103e-6654.4778 kDa glucose-regulated protein OS=Homo sapiens G... [more]
GRP78_BOVIN1.103e-6654.4778 kDa glucose-regulated protein OS=Bos taurus GN=... [more]
BIP5_TOBAC1.441e-6654.89Luminal-binding protein 5 OS=Nicotiana tabacum GN=... [more]
GRP78_RAT3.211e-6654.0478 kDa glucose-regulated protein OS=Rattus norvegi... [more]
GRP78_MOUSE3.211e-6654.0478 kDa glucose-regulated protein OS=Mus musculus G... [more]
GRP78_MESAU3.211e-6654.0478 kDa glucose-regulated protein OS=Mesocricetus a... [more]
GRP78_CHICK3.211e-6654.0478 kDa glucose-regulated protein OS=Gallus gallus ... [more]
GRP78_CANGA4.193e-6655.3278 kDa glucose-regulated protein homolog OS=Candid... [more]
BIP_SOLLC4.193e-6654.89Luminal-binding protein OS=Solanum lycopersicum PE... [more]

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Properties
Property NameValue
Genbank descriptionEST0007 Mature fruit abscission zone cDNA subtraction library Citrus sinensis cDNA clone MFAZ007 similar to 70 kDa heat shock protein (acc# P27322), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB250245 ID=CB250245; Name=CB250245; organism=Citrus sinensis; type=EST; length=709bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACCGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACTGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCCTGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGG
TTCTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAG
GACTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGC
GGTTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTA
ATGAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTG
GGTTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACAC
CACTATTCCAACCAAGAATGAGCAGGTGTTCTCCACATACTCTGACAACC
AGCCCGGGGTGTTGATCCAGGTCTACGAGGGTGAGCGCACAAGAACCCGT
GACAACAACTTGCTTGGAAAGTTTGATCCCTCTGGCATTCCTCCTGCTCC
AAGAGGGGT
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