CB250245 | Citrus Genome Database

Overview


Name	CB250245
Unique Name	CB250245
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	709

Libraries


Library Name	Type
Mature fruit abscission zone cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13846	contig	Csv1_Contig13846:683..1391.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7D_MANSE (Heat shock 70 kDa protein cognate 4 OS=Manduca sexta PE=2 SV=1)

HSP 1 Score: 340.117 bits (871), Expect = 6.864e-93
Identity = 173/235 (73.62%), Postives = 190/235 (80.85%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHFVQEFKRK KKD++ N RALRRLRTACERAKRTLSS+ Q +IEIDSL+EGIDFYT+ITRARFEELN DLFR  MEPVEK LRDAKMDKS +HD+VLVGGSTRIPKVQ+LLQDFFNGKEL KSINPDE           IL G+ +E+VQD             ETAGGVMT LI RNTTIPTK  Q F+TYSDNQPGVLIQV+EGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFVQEFKRKYKKDLTTNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKSQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILHGDKSEEVQDLLLLDVTPLSLGIETAGGVMTTLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVFEGERAMTKDNNLLGKFELTGIPPAPRG 470

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_CRIGR (Heat shock cognate 71 kDa protein OS=Cricetulus griseus GN=HSPA8 PE=2 SV=1)

HSP 1 Score: 339.347 bits (869), Expect = 1.171e-92
Identity = 171/235 (72.77%), Postives = 190/235 (80.85%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKR +KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK LRDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRNDKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_CHICK (Heat shock cognate 71 kDa protein OS=Gallus gallus GN=HSPA8 PE=1 SV=1)

HSP 1 Score: 338.576 bits (867), Expect = 1.997e-92
Identity = 170/235 (72.34%), Postives = 191/235 (81.28%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFE+LN DLFR  ++PVEK LRDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RLVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEKLNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP71_ORYLA (Heat shock 70 kDa protein 1 OS=Oryzias latipes PE=3 SV=1)

HSP 1 Score: 337.806 bits (865), Expect = 3.406e-92
Identity = 172/235 (73.19%), Postives = 190/235 (80.85%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHFV+EFKRK+KKDIS N RALRRLRTACERAKRTLSS++Q +IEIDSL+EGIDFYT++TRARFEEL  DLFR  +EPVEK LRDAKMDK  +HDVVLVGGSTRIPK+Q+LLQDFFNG+EL KSINPDE           ILSG+ +  VQD             ETAGGVMT LI RNTTIPTK  QVFSTY+DNQPGVLIQVYEGER  T+DNNLLGKFD +GIPPAPRG
Sbjct:  238 RMVNHFVEEFKRKHKKDISQNKRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTSVTRARFEELCSDLFRGTLEPVEKALRDAKMDKGQIHDVVLVGGSTRIPKIQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAILSGDTSGNVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKQTQVFSTYADNQPGVLIQVYEGERAMTKDNNLLGKFDLTGIPPAPRG 472

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS7C1_DICDI (Heat shock cognate 70 kDa protein 1 OS=Dictyostelium discoideum GN=hspB PE=1 SV=2)

HSP 1 Score: 337.035 bits (863), Expect = 5.810e-92
Identity = 170/235 (72.34%), Postives = 189/235 (80.43%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV EFKRK+KKDI GN RA+RRLRTACERAKRTLSS+AQ +IEIDSL+EGIDFYT+ITRARFEEL  DLFR C++PVEK L+D+K+DK S+H++VLVGGSTRIPKVQQLLQ+FFNGKEL KSINPDE           ILS EG  KV D             ETAGGVMT LIPRNTTIP K  Q FSTYSDNQPGVLIQVYEGER  T+DNNLLGKF+ SGIPPAPRG
Sbjct:  234 RLVSHFVDEFKRKHKKDIMGNQRAVRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRGCLDPVEKVLKDSKLDKKSIHEIVLVGGSTRIPKVQQLLQEFFNGKELNKSINPDEAVAYGAAVQAAILSNEGGAKVADLLLLDVAPLSMGLETAGGVMTTLIPRNTTIPCKKTQTFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRG 468

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP72_SCHPO (Probable heat shock protein ssa2 OS=Schizosaccharomyces pombe GN=ssa2 PE=1 SV=3)

HSP 1 Score: 335.88 bits (860), Expect = 1.294e-91
Identity = 167/235 (71.06%), Postives = 194/235 (82.55%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHF+QEFKRKNKKDI+GN RA+RRLRTACERAKRTLSS+AQ +IEIDSL+EGIDFYT+ITRARFEEL  DLFRK MEPVE+ LRD+K+DK+SV+++VLVGGSTRIP+VQ+L+ DFFNGKE CKSINPDE           +L+G+ +EK QD             ETAGGVMT LI RNTTIPTK  ++FSTYSDNQPGVLIQV+EGER RT+D NLLGKF+ SGIPPAPRG
Sbjct:  234 RLVNHFIQEFKRKNKKDITGNARAVRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRKTMEPVERVLRDSKVDKASVNEIVLVGGSTRIPRVQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAVLTGDTSEKTQDLLLLDVAPLSMGIETAGGVMTPLIKRNTTIPTKKSEIFSTYSDNQPGVLIQVFEGERARTKDCNLLGKFELSGIPPAPRG 468

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP71_PUCGR (Heat shock protein HSS1 OS=Puccinia graminis GN=HSS1 PE=3 SV=1)

HSP 1 Score: 335.88 bits (860), Expect = 1.294e-91
Identity = 166/235 (70.64%), Postives = 192/235 (81.70%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFVQEFKRK+KKD+S NPRALRRLRTACERAKRTLSS AQTTIEIDSL+EG+DFYT++TRARFEEL  DLFR  +EPVEK LRDAK+DK++VH++VLVGGSTRIP++ +L+ DFFNGKE  KSINPDE           IL+G+ +EK QD             ETAGGVMT LI RNTT+PTK  ++FSTYSDNQPGVLIQVYEGER RT+DNNLLGKF+ +GIPPAPRG
Sbjct:  234 RLVNHFVQEFKRKHKKDLSSNPRALRRLRTACERAKRTLSSAAQTTIEIDSLFEGVDFYTSLTRARFEELCQDLFRSTLEPVEKVLRDAKIDKAAVHEIVLVGGSTRIPRIVKLVSDFFNGKEPNKSINPDEAVAYGAAVQAAILTGDTSEKTQDLLLLDVSPLSLGIETAGGVMTPLIKRNTTVPTKKSEIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELAGIPPAPRG 468

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP72_PICAN (Heat shock protein 70 2 OS=Pichia angusta GN=HSA2 PE=3 SV=2)

HSP 1 Score: 335.109 bits (858), Expect = 2.208e-91
Identity = 168/235 (71.49%), Postives = 190/235 (80.85%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHF+ EFKRKNKKDI GN RALRRLRTACERAK TLSS+AQT++EIDSLYEGIDFYT+ITRARFEEL  DLFR  ++PVEK L+DAK+DKS V+++VLVGGSTRIPKVQ+L+ DFFNGKE  KSINPDE           IL+G+ + K QD             ETAGGVMT LIPRNTTIPTK  ++FSTYSDNQPGVLIQVYEGER RT+DNNLLGKF+ SGIPPAPRG
Sbjct:  234 RLVNHFINEFKRKNKKDICGNQRALRRLRTACERAKTTLSSSAQTSLEIDSLYEGIDFYTSITRARFEELCQDLFRSTLDPVEKVLKDAKLDKSQVNEIVLVGGSTRIPKVQKLVSDFFNGKEPNKSINPDEAVAYGAAVQAAILTGDTSSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNTTIPTKKSEIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRG 468

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP71_SCHPO (Probable heat shock protein ssa1 OS=Schizosaccharomyces pombe GN=ssa1 PE=1 SV=2)

HSP 1 Score: 334.724 bits (857), Expect = 2.884e-91
Identity = 169/235 (71.91%), Postives = 191/235 (81.28%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHF QEFKRKNKKDI+GN RA+RRLRTACERAKRTLSS+AQ +IEIDSLYEGIDFYT+ITRARFEEL  DLFR  MEPVEK LRD+K+DKSSV+++VLVGGSTRIP++Q+L+ DFFNGKE CKSINPDE           IL G+ +EK QD             ETAGGVMT LI RNTTIPTK  +VFSTY+DNQPGVLIQV+EGER RT+D NLLGKF+ SGIPPAPRG
Sbjct:  234 RLVNHFAQEFKRKNKKDITGNARAVRRLRTACERAKRTLSSSAQASIEIDSLYEGIDFYTSITRARFEELCADLFRNTMEPVEKVLRDSKIDKSSVNEIVLVGGSTRIPRIQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAILVGDTSEKTQDLLLLDVAPLSLGIETAGGVMTPLIKRNTTIPTKKSEVFSTYADNQPGVLIQVFEGERARTKDCNLLGKFELSGIPPAPRG 468

[Close]

BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP70_BRUMA (Heat shock 70 kDa protein OS=Brugia malayi GN=HSP70 PE=3 SV=1)

HSP 1 Score: 334.724 bits (857), Expect = 2.884e-91
Identity = 170/235 (72.34%), Postives = 188/235 (80.00%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHFV EFKR +KKD++ NPRALRRLRTACERAKRTLSS++Q +IEIDSL+EGIDFYT ITRARFEEL  DLFR  M+PVEK LRDAKMDK+ VHD+VLVGGSTRIPKVQ+LL DFF+GKEL KSINPDE           ILSG+ +E VQD             ETAGGVMT LI RNTTIPTK  + F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ SGIPPAPRG
Sbjct:  235 RMVNHFVAEFKRNDKKDLASNPRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTNITRARFEELCADLFRSTMDPVEKALRDAKMDKAQVHDIVLVGGSTRIPKVQKLLSDFFSGKELNKSINPDEAVAYGAAVQAAILSGDKSEAVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKTSETFTTYSDNQPGVLIQVYEGERALTKDNNLLGKFELSGIPPAPRG 469

The following BLAST results are available for this feature:

BLAST of CB250245 vs. ExPASy Swiss-Prot

BLAST of CB250245 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP7D_MANSE	6.864e-93	73.62	Heat shock 70 kDa protein cognate 4 OS=Manduca sex...	[more]
HSP7C_CRIGR	1.171e-92	72.77	Heat shock cognate 71 kDa protein OS=Cricetulus gr...	[more]
HSP7C_CHICK	1.997e-92	72.34	Heat shock cognate 71 kDa protein OS=Gallus gallus...	[more]
HSP71_ORYLA	3.406e-92	73.19	Heat shock 70 kDa protein 1 OS=Oryzias latipes PE=...	[more]
HS7C1_DICDI	5.810e-92	72.34	Heat shock cognate 70 kDa protein 1 OS=Dictyosteli...	[more]
HSP72_SCHPO	1.294e-91	71.06	Probable heat shock protein ssa2 OS=Schizosaccharo...	[more]
HSP71_PUCGR	1.294e-91	70.64	Heat shock protein HSS1 OS=Puccinia graminis GN=HS...	[more]
HSP72_PICAN	2.208e-91	71.49	Heat shock protein 70 2 OS=Pichia angusta GN=HSA2 ...	[more]
HSP71_SCHPO	2.884e-91	71.91	Probable heat shock protein ssa1 OS=Schizosaccharo...	[more]
HSP70_BRUMA	2.884e-91	72.34	Heat shock 70 kDa protein OS=Brugia malayi GN=HSP7...	[more]

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Properties


Property Name	Value
Genbank description	EST0007 Mature fruit abscission zone cDNA subtraction library Citrus sinensis cDNA clone MFAZ007 similar to 70 kDa heat shock protein (acc# P27322), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB250245 ID=CB250245; Name=CB250245; organism=Citrus sinensis; type=EST; length=709bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACCGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACTGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCCTGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGG
TTCTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAG
GACTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGC
GGTTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTA
ATGAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTG
GGTTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACAC
CACTATTCCAACCAAGAATGAGCAGGTGTTCTCCACATACTCTGACAACC
AGCCCGGGGTGTTGATCCAGGTCTACGAGGGTGAGCGCACAAGAACCCGT
GACAACAACTTGCTTGGAAAGTTTGATCCCTCTGGCATTCCTCCTGCTCC
AAGAGGGGT