CB250245

Overview
NameCB250245
Unique NameCB250245
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length709
Libraries
Library NameType
Mature fruit abscission zone cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71L_HUMAN (Heat shock 70 kDa protein 1-like OS=Homo sapiens GN=HSPA1L PE=1 SV=2)

HSP 1 Score: 331.643 bits (849), Expect = 2.441e-90
Identity = 166/235 (70.64%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  T+DNNLLG+FD +GIPPAPRG
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALRDAKMDKAKIHDIVLVGGSTRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFDLTGIPPAPRG 472
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP71_CANFA (Heat shock 70 kDa protein 1 OS=Canis familiaris GN=HSPA1 PE=2 SV=1)

HSP 1 Score: 331.257 bits (848), Expect = 3.188e-90
Identity = 166/235 (70.64%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71B_PIG (Heat shock 70 kDa protein 1B OS=Sus scrofa GN=HSPA1B PE=2 SV=1)

HSP 1 Score: 331.257 bits (848), Expect = 3.188e-90
Identity = 166/235 (70.64%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71B_BOVIN (Heat shock 70 kDa protein 1B OS=Bos taurus GN=HSPA1B PE=2 SV=1)

HSP 1 Score: 331.257 bits (848), Expect = 3.188e-90
Identity = 166/235 (70.64%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71B_BOSMU (Heat shock 70 kDa protein 1B OS=Bos mutus grunniens GN=HSPA1B PE=2 SV=1)

HSP 1 Score: 331.257 bits (848), Expect = 3.188e-90
Identity = 166/235 (70.64%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71A_BOVIN (Heat shock 70 kDa protein 1A OS=Bos taurus GN=HSPA1A PE=2 SV=2)

HSP 1 Score: 331.257 bits (848), Expect = 3.188e-90
Identity = 166/235 (70.64%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7C_ICTPU (Heat shock cognate 71 kDa protein OS=Ictalurus punctatus GN=hsc70 PE=2 SV=1)

HSP 1 Score: 330.487 bits (846), Expect = 5.438e-90
Identity = 167/235 (71.06%), Postives = 187/235 (79.57%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF+ EFKRK+KKDIS N RA+RRL TACERAKRTLSS+ Q +IEIDSLYEG+DFYT+ITRARFEELN DLFR  ++PVEK LRDAKMDK+ VHD+VLVGGSTRIPK+++LLQDFFNGKEL KSINPDE              G+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ +GIPPAPRG
Sbjct:  236 RMVNHFIAEFKRKHKKDISDNKRAVRRLATACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLDPVEKALRDAKMDKAQVHDIVLVGGSTRIPKMEKLLQDFFNGKELNKSINPDEAVAYGAAVQAESSLGDKSENVQDLVLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP76_HUMAN (Heat shock 70 kDa protein 6 OS=Homo sapiens GN=HSPA6 PE=1 SV=2)

HSP 1 Score: 330.102 bits (845), Expect = 7.103e-90
Identity = 165/235 (70.21%), Postives = 187/235 (79.57%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHF++EF+RK+ KD+SGN RALRRLRTACERAKRTLSS+ Q T+EIDSL+EG+DFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HDVVLVGGSTRIPKVQ+LLQDFFNGKEL KSINPDE           +L G+  EKVQD             ETAGGVMT LI RN TIPTK  Q F+TYSDNQPGV IQVYEGER  T+DNNLLG+F+ SGIPPAPRG
Sbjct:  238 RLVNHFMEEFRRKHGKDLSGNKRALRRLRTACERAKRTLSSSTQATLEIDSLFEGVDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDVVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAVLMGDKCEKVQDLLLLDVAPLSLGLETAGGVMTTLIQRNATIPTKQTQTFTTYSDNQPGVFIQVYEGERAMTKDNNLLGRFELSGIPPAPRG 472
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP71_PONAB (Heat shock 70 kDa protein 1 OS=Pongo abelii GN=HSPA1 PE=2 SV=1)

HSP 1 Score: 330.102 bits (845), Expect = 7.103e-90
Identity = 165/235 (70.21%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  T+DNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP71_HUMAN (Heat shock 70 kDa protein 1A/1B OS=Homo sapiens GN=HSPA1A PE=1 SV=5)

HSP 1 Score: 330.102 bits (845), Expect = 7.103e-90
Identity = 165/235 (70.21%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  T+DNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRG 470
The following BLAST results are available for this feature:
BLAST of CB250245 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HS71L_HUMAN2.441e-9070.64Heat shock 70 kDa protein 1-like OS=Homo sapiens G... [more]
HSP71_CANFA3.188e-9070.64Heat shock 70 kDa protein 1 OS=Canis familiaris GN... [more]
HS71B_PIG3.188e-9070.64Heat shock 70 kDa protein 1B OS=Sus scrofa GN=HSPA... [more]
HS71B_BOVIN3.188e-9070.64Heat shock 70 kDa protein 1B OS=Bos taurus GN=HSPA... [more]
HS71B_BOSMU3.188e-9070.64Heat shock 70 kDa protein 1B OS=Bos mutus grunnien... [more]
HS71A_BOVIN3.188e-9070.64Heat shock 70 kDa protein 1A OS=Bos taurus GN=HSPA... [more]
HSP7C_ICTPU5.438e-9071.06Heat shock cognate 71 kDa protein OS=Ictalurus pun... [more]
HSP76_HUMAN7.103e-9070.21Heat shock 70 kDa protein 6 OS=Homo sapiens GN=HSP... [more]
HSP71_PONAB7.103e-9070.21Heat shock 70 kDa protein 1 OS=Pongo abelii GN=HSP... [more]
HSP71_HUMAN7.103e-9070.21Heat shock 70 kDa protein 1A/1B OS=Homo sapiens GN... [more]

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Properties
Property NameValue
Genbank descriptionEST0007 Mature fruit abscission zone cDNA subtraction library Citrus sinensis cDNA clone MFAZ007 similar to 70 kDa heat shock protein (acc# P27322), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB250245 ID=CB250245; Name=CB250245; organism=Citrus sinensis; type=EST; length=709bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACCGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACTGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCCTGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGG
TTCTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAG
GACTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGC
GGTTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTA
ATGAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTG
GGTTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACAC
CACTATTCCAACCAAGAATGAGCAGGTGTTCTCCACATACTCTGACAACC
AGCCCGGGGTGTTGATCCAGGTCTACGAGGGTGAGCGCACAAGAACCCGT
GACAACAACTTGCTTGGAAAGTTTGATCCCTCTGGCATTCCTCCTGCTCC
AAGAGGGGT
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