CB250245

Overview
NameCB250245
Unique NameCB250245
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length709
Libraries
Library NameType
Mature fruit abscission zone cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13846 contig Csv1_Contig13846:683..1391. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7A_DROME (Heat shock 70 kDa protein cognate 1 OS=Drosophila melanogaster GN=Hsc70-1 PE=1 SV=1)

HSP 1 Score: 329.331 bits (843), Expect = 1.212e-89
Identity = 166/235 (70.64%), Postives = 186/235 (79.15%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFVQEF+RK+KKD+  N RALRRLRTACERAKRTLSS+ Q +IEIDSL+EG+DFYT++TRARFEELN DLFR  MEPV K LRDAKMDK  +HD+VLVGGSTRIPKVQ+LLQDFFNGKEL KSINPDE           IL G+ +E VQD             ETAGGVMT LI RNTTIPTK  Q+F+TY+DNQPGVLIQV+EGER  TRDNN LGKF+ S IPPAPRG
Sbjct:  236 RLVNHFVQEFQRKHKKDLGQNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGVDFYTSVTRARFEELNGDLFRGTMEPVAKALRDAKMDKGQIHDIVLVGGSTRIPKVQRLLQDFFNGKELNKSINPDEAVAYGAAVQAAILHGDKSEAVQDLLLLDVTPLSLGIETAGGVMTTLIKRNTTIPTKQTQIFTTYADNQPGVLIQVFEGERAMTRDNNSLGKFELSAIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP76_PIG (Heat shock 70 kDa protein 6 OS=Sus scrofa GN=HSPA6 PE=2 SV=1)

HSP 1 Score: 329.331 bits (843), Expect = 1.212e-89
Identity = 163/235 (69.36%), Postives = 188/235 (80.00%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHF++EF+RK++KD+S N RALRRLRTACERAKRTLSS+ Q T+EIDSL+EG+DFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPK+Q+LLQDFFNG+EL KSINPDE           +L G+  EKVQD             ETAGGVMT LI RN TIPTK  Q F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  238 RLVNHFMEEFRRKHRKDLSRNKRALRRLRTACERAKRTLSSSTQATLEIDSLFEGVDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDIVLVGGSTRIPKIQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAVLMGDKCEKVQDLLLLDVAPLSLGLETAGGVMTTLIQRNATIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 472
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71A_PIG (Heat shock 70 kDa protein 1A OS=Sus scrofa GN=HSPA1A PE=3 SV=1)

HSP 1 Score: 329.331 bits (843), Expect = 1.212e-89
Identity = 165/235 (70.21%), Postives = 189/235 (80.43%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFV+EFKRK+KKD S N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVNHFVEEFKRKHKKDYSQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP72_YEAST (Heat shock protein SSA2 OS=Saccharomyces cerevisiae GN=SSA2 PE=1 SV=3)

HSP 1 Score: 328.561 bits (841), Expect = 2.067e-89
Identity = 164/235 (69.79%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHF+QEFKRKNKKD+S N RALRRLRTACERAKRTLSS+AQT++EIDSL+EGIDFYT+ITRARFEEL  DLFR  ++PVEK LRDAK+DKS V ++VLVGGSTRIPKVQ+L+ D+FNGKE  +SINPDE           IL+G+ + K QD             ETAGGVMT LIPRN+TIPTK  +VFSTY+DNQPGVLIQV+EGER +T+DNNLLGKF+ SGIPPAPRG
Sbjct:  233 RLVNHFIQEFKRKNKKDLSTNQRALRRLRTACERAKRTLSSSAQTSVEIDSLFEGIDFYTSITRARFEELCADLFRSTLDPVEKVLRDAKLDKSQVDEIVLVGGSTRIPKVQKLVTDYFNGKEPNRSINPDEAVAYGAAVQAAILTGDESSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNSTIPTKKSEVFSTYADNQPGVLIQVFEGERAKTKDNNLLGKFELSGIPPAPRG 467
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP70_SCHMA (Heat shock 70 kDa protein homolog OS=Schistosoma mansoni PE=2 SV=2)

HSP 1 Score: 328.561 bits (841), Expect = 2.067e-89
Identity = 169/235 (71.91%), Postives = 185/235 (78.72%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMV+HFV+EF++K  KDI  N RALRRLRTACERAKRTLSS+AQT +EIDSL +G DFYT ITRARFEELN DLFR  ++PVEK LRDAKMDKS +HD+VLVGGSTRIPKVQ+LLQDFFNGKEL KSINPDE           ILSGE  E VQD             ETAGGVMT LI RNTTIPTK  Q F+TYSDNQPGVLIQV+EGER  T+DNNLLGKF+ SGIPPAPRG
Sbjct:  234 RMVDHFVKEFQKKYNKDIRSNKRALRRLRTACERAKRTLSSSAQTNLEIDSLCDGTDFYTVITRARFEELNADLFRGTLDPVEKALRDAKMDKSQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGEKCEAVQDLLLLDVAPLSLGLETAGGVMTALIKRNTTIPTKQTQTFTTYSDNQPGVLIQVFEGERALTKDNNLLGKFELSGIPPAPRG 468
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71B_MOUSE (Heat shock 70 kDa protein 1B OS=Mus musculus GN=Hspa1b PE=1 SV=3)

HSP 1 Score: 328.561 bits (841), Expect = 2.067e-89
Identity = 166/235 (70.64%), Postives = 189/235 (80.43%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRGTLEPVEKALRDAKMDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71A_MOUSE (Heat shock 70 kDa protein 1A OS=Mus musculus GN=Hspa1a PE=1 SV=2)

HSP 1 Score: 328.561 bits (841), Expect = 2.067e-89
Identity = 166/235 (70.64%), Postives = 189/235 (80.43%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRGTLEPVEKALRDAKMDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP71_YEAST (Heat shock protein SSA1 OS=Saccharomyces cerevisiae GN=SSA1 PE=1 SV=4)

HSP 1 Score: 328.176 bits (840), Expect = 2.699e-89
Identity = 163/235 (69.36%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHF+QEFKRKNKKD+S N RALRRLRTACERAKRTLSS+AQT++EIDSL+EGIDFYT+ITRARFEEL  DLFR  ++PVEK LRDAK+DKS V ++VLVGGSTRIPKVQ+L+ D+FNGKE  +SINPDE           IL+G+ + K QD             ETAGGVMT LIPRN+TIPTK  ++FSTY+DNQPGVLIQV+EGER +T+DNNLLGKF+ SGIPPAPRG
Sbjct:  233 RLVNHFIQEFKRKNKKDLSTNQRALRRLRTACERAKRTLSSSAQTSVEIDSLFEGIDFYTSITRARFEELCADLFRSTLDPVEKVLRDAKLDKSQVDEIVLVGGSTRIPKVQKLVTDYFNGKEPNRSINPDEAVAYGAAVQAAILTGDESSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNSTIPTKKSEIFSTYADNQPGVLIQVFEGERAKTKDNNLLGKFELSGIPPAPRG 467
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP71_RAT (Heat shock 70 kDa protein 1A/1B OS=Rattus norvegicus GN=Hspa1a PE=2 SV=2)

HSP 1 Score: 327.405 bits (838), Expect = 4.604e-89
Identity = 165/235 (70.21%), Postives = 189/235 (80.43%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK  Q F+TYSDNQPGVLIQVYEGER  TRDNNLLG+F+ SGIPPAPRG
Sbjct:  236 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRGTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS7C2_DICDI (Heat shock cognate 70 kDa protein 2 OS=Dictyostelium discoideum GN=hspE-1 PE=1 SV=1)

HSP 1 Score: 327.405 bits (838), Expect = 4.604e-89
Identity = 165/235 (70.21%), Postives = 185/235 (78.72%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFV EFKRK+KKD+  N RALRRLRTACERAKRTLSS+AQ +IEIDSL+EGIDFYT+ITRARFEEL  DLFR C++PV+K L+D+K+DK ++H++VLVGGSTRIPKVQQLLQD FNGKEL KSINPDE           ILS EG  KV D             ETAGGVMT LIPRNTTIP K  Q FSTYSDNQ GVL+QVYEGER  TRDNNLLGKF+ + IPPAPRG
Sbjct:  235 RLVNHFVDEFKRKHKKDLMTNQRALRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRGCLDPVDKVLKDSKLDKKTIHEIVLVGGSTRIPKVQQLLQDHFNGKELNKSINPDEAVAYGAAVQAAILSNEGGAKVADILLLDVAPLSMGLETAGGVMTTLIPRNTTIPCKKNQTFSTYSDNQTGVLVQVYEGERAMTRDNNLLGKFELTNIPPAPRG 469
The following BLAST results are available for this feature:
BLAST of CB250245 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HSP7A_DROME1.212e-8970.64Heat shock 70 kDa protein cognate 1 OS=Drosophila ... [more]
HSP76_PIG1.212e-8969.36Heat shock 70 kDa protein 6 OS=Sus scrofa GN=HSPA6... [more]
HS71A_PIG1.212e-8970.21Heat shock 70 kDa protein 1A OS=Sus scrofa GN=HSPA... [more]
HSP72_YEAST2.067e-8969.79Heat shock protein SSA2 OS=Saccharomyces cerevisia... [more]
HSP70_SCHMA2.067e-8971.91Heat shock 70 kDa protein homolog OS=Schistosoma m... [more]
HS71B_MOUSE2.067e-8970.64Heat shock 70 kDa protein 1B OS=Mus musculus GN=Hs... [more]
HS71A_MOUSE2.067e-8970.64Heat shock 70 kDa protein 1A OS=Mus musculus GN=Hs... [more]
HSP71_YEAST2.699e-8969.36Heat shock protein SSA1 OS=Saccharomyces cerevisia... [more]
HSP71_RAT4.604e-8970.21Heat shock 70 kDa protein 1A/1B OS=Rattus norvegic... [more]
HS7C2_DICDI4.604e-8970.21Heat shock cognate 70 kDa protein 2 OS=Dictyosteli... [more]

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Properties
Property NameValue
Genbank descriptionEST0007 Mature fruit abscission zone cDNA subtraction library Citrus sinensis cDNA clone MFAZ007 similar to 70 kDa heat shock protein (acc# P27322), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB250245 ID=CB250245; Name=CB250245; organism=Citrus sinensis; type=EST; length=709bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACCGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACTGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCCTGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGG
TTCTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAG
GACTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGC
GGTTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTA
ATGAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTG
GGTTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACAC
CACTATTCCAACCAAGAATGAGCAGGTGTTCTCCACATACTCTGACAACC
AGCCCGGGGTGTTGATCCAGGTCTACGAGGGTGAGCGCACAAGAACCCGT
GACAACAACTTGCTTGGAAAGTTTGATCCCTCTGGCATTCCTCCTGCTCC
AAGAGGGGT
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