CB304915

Overview
NameCB304915
Unique NameCB304915
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length701
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CB304915_ssr284 microsatellite CB304915_ssr284:284..293. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig2077 contig Csv1_Contig2077:158..858. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HS16B_WHEAT (16.9 kDa class I heat shock protein 2 OS=Triticum aestivum GN=hsp16.9B PE=1 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 8.427e-19
Identity = 48/98 (48.98%), Postives = 67/98 (68.37%), Query Frame = -2
Query:  359 DAKAMAATPADVKEYPNSYVFIVDMPGLKSGDIKVQVEDDNVLPISGERKREEEKDGAKYVRMERRVGKFMRKFVLPENANVEAISAVCQDGVLTVTV 652
            +  A A    D KE P ++VF  D+PG+K  ++KV+VED NVL +SGER +E+E    K+ R+ER  GKF+R+F L E+A VE + A  ++GVLTVTV
Sbjct:   37 ETAAFANARMDWKETPEAHVFKADLPGVKKEEVKVEVEDGNVLVVSGERTKEKEDKNDKWHRVERSSGKFVRRFRLLEDAKVEEVKAGLENGVLTVTV 134          
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HSP22_ARATH (22.0 kDa heat shock protein OS=Arabidopsis thaliana GN=HSP22.0 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 42/95 (44.21%), Postives = 68/95 (71.58%), Query Frame = -2
Query:  359 AMAATPADVKEYPNSYVFIVDMPGLKSGDIKVQVEDDNVLPISGERKREEEKDGAKYVRMERRVGKFMRKFVLPENANVEAISAVCQDGVLTVTV 643
            A++    D KE    +  ++D+PGLK  ++K++VE++ VL +SGERKREEEK G ++ R+ER  GKF R+F LP+N ++E++ A  ++GVLT+ +
Sbjct:   67 ALSPARVDWKETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWRQFKLPDNVDMESVKAKLENGVLTINL 161          
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HSP11_HELAN (17.6 kDa class I heat shock protein OS=Helianthus annuus GN=HSP17.6 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 46/99 (46.46%), Postives = 67/99 (67.68%), Query Frame = -2
Query:  359 RDAKAMAATPADVKEYPNSYVFIVDMPGLKSGDIKVQVEDDNVLPISGERKREEEKDGAKYVRMERRVGKFMRKFVLPENANVEAISAVCQDGVLTVTV 655
            R+  A+     D KE P ++V   D+PG+K  ++KV+VED  VL ISGER RE+E+    + R+ER  GKF+R+F LPENA ++ + A+ ++GVLTV V
Sbjct:   37 RETAAIVNARIDWKETPEAHVLKADLPGMKKEEVKVEVEDGRVLQISGERCREQEEKDDTWHRVERSSGKFIRRFRLPENAKMDEVKAMMENGVLTVVV 135          
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HSP11_SOYBN (17.3 kDa class I heat shock protein OS=Glycine max GN=HSP17.3-B PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 7.134e-18
Identity = 45/95 (47.37%), Postives = 64/95 (67.37%), Query Frame = -2
Query:  359 AMAATPADVKEYPNSYVFIVDMPGLKSGDIKVQVEDDNVLPISGERKREEEKDGAKYVRMERRVGKFMRKFVLPENANVEAISAVCQDGVLTVTV 643
            A  +T  D KE P ++VF  D+PGLK  ++K++++D  VL ISGER  E+E     + R+ER  GK +R+F LPENA V+ + A  ++GVLTVTV
Sbjct:   42 AFVSTRVDWKETPEAHVFKADIPGLKKEEVKLEIQDGRVLQISGERNVEKEDKNDTWHRVERSSGKLVRRFRLPENAKVDQVKASMENGVLTVTV 136          
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HS17B_ARATH (17.6 kDa class I heat shock protein 2 OS=Arabidopsis thaliana GN=HSP17.6B PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 7.134e-18
Identity = 44/99 (44.44%), Postives = 66/99 (66.67%), Query Frame = -2
Query:  359 RDAKAMAATPADVKEYPNSYVFIVDMPGLKSGDIKVQVEDDNVLPISGERKREEEKDGAKYVRMERRVGKFMRKFVLPENANVEAISAVCQDGVLTVTV 655
            R+  A+     D +E P ++VF  D+PGLK  ++KV++E+D+VL ISGER  E+E     + R+ER  G+F R+F LPEN  ++ + A  ++GVLTVTV
Sbjct:   38 RENSAIVNARVDWRETPEAHVFKADLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAMENGVLTVTV 136          
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HS178_ARATH (17.8 kDa class I heat shock protein OS=Arabidopsis thaliana GN=HSP17.8 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.076e-17
Identity = 44/98 (44.90%), Postives = 65/98 (66.33%), Query Frame = -2
Query:  359 DAKAMAATPADVKEYPNSYVFIVDMPGLKSGDIKVQVEDDNVLPISGERKREEEKDGAKYVRMERRVGKFMRKFVLPENANVEAISAVCQDGVLTVTV 652
            +  A+     D KE   ++VF  D+PG+K  ++KV++EDD+VL ISGER  E+E+    + R+ER  G+F RKF LPEN  ++ + A  ++GVLTVTV
Sbjct:   41 ETSAITNARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASMENGVLTVTV 138          
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HS17A_ARATH (17.6 kDa class I heat shock protein 1 OS=Arabidopsis thaliana GN=HSP17.6A PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.711e-17
Identity = 45/97 (46.39%), Postives = 65/97 (67.01%), Query Frame = -2
Query:  359 AKAMAATPADVKEYPNSYVFIVDMPGLKSGDIKVQVEDDNVLPISGERKREEEKDGAKYVRMERRVGKFMRKFVLPENANVEAISAVCQDGVLTVTV 649
            + A+A    D KE   ++VF  D+PG+K  ++KV++EDD+VL ISGER  E+E+    + R+ER  G F RKF LPEN  ++ + A  ++GVLTVTV
Sbjct:   40 SSAIANARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMDQVKASMENGVLTVTV 136          
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HS166_ORYSJ (16.6 kDa heat shock protein OS=Oryza sativa subsp. japonica GN=HSP16.6 PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 7.887e-17
Identity = 46/91 (50.55%), Postives = 60/91 (65.93%), Query Frame = -2
Query:  359 DVKEYPNSYVFIVDMPGLKSGDIKVQVEDDNVLPISGERKREEEKDG---AKYVRMERRVGKFMRKFVLPENANVEAISAVCQDGVLTVTV 622
            D KE P ++VF  D+PG++    KV+VED  VL ISGER REE+ DG    ++  +ER  GKF R+F LP  A V+ +SA   +GVLTVTV
Sbjct:   43 DWKETPTAHVFTADLPGVRKDQAKVEVEDGGVLVISGERAREEDVDGKNDERWHHVERSSGKFQRRFRLPRGARVDQVSASMDNGVLTVTV 133          
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HS219_ORYSJ (21.9 kDa heat shock protein OS=Oryza sativa subsp. japonica GN=HSP21.9 PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 6.677e-16
Identity = 45/103 (43.69%), Postives = 67/103 (65.05%), Query Frame = -2
Query:  359 AMAATPADVKEYPNSYVFIVDMPGLKSGDIKVQVED-DNVLPISGERKR-------EEEKDGAKYVRMERRVGKFMRKFVLPENANVEAISAVCQDGVLTVTV 643
            A+A    D KE P ++V  VD+PG++ GD++V+V++   VL +SGER+R       E E+DG ++ R ER  G+F R+F +P  A+V  ++A   DGVLTVTV
Sbjct:   68 AVALARCDWKETPEAHVVTVDVPGVRRGDVRVEVDEASRVLRVSGERRRAGAAEEEEGERDGVRWHRAERAAGRFWRRFRMPPGADVGRVAARLDDGVLTVTV 170          
BLAST of CB304915 vs. ExPASy Swiss-Prot
Match: HS174_ARATH (17.4 kDa class III heat shock protein OS=Arabidopsis thaliana GN=HSP17.4B PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 8.720e-16
Identity = 41/95 (43.16%), Postives = 69/95 (72.63%), Query Frame = -2
Query:  359 PADVKEYPNSYVFIVDMPGLKSGDIKVQVEDDNVLPI--SGERKR--EEEKDGAKYVRMERRVGK-FMRKFVLPENANVEAISAVCQDGVLTVTV 628
            P D+ E P  Y+F +D+PG+   DI+V VE++  L I  +G+RKR  +E ++G+KY+R+ERR+ +  ++KF LPE+A++ +++A  Q+GVLTV +
Sbjct:   43 PIDILESPKEYIFYLDIPGISKSDIQVTVEEERTLVIKSNGKRKRDDDESEEGSKYIRLERRLAQNLVKKFRLPEDADMASVTAKYQEGVLTVVI 137          
The following BLAST results are available for this feature:
BLAST of CB304915 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 55
Match NameE-valueIdentityDescription
HS16B_WHEAT8.427e-1948.9816.9 kDa class I heat shock protein 2 OS=Triticum ... [more]
HSP22_ARATH1.437e-1844.2122.0 kDa heat shock protein OS=Arabidopsis thalian... [more]
HSP11_HELAN1.437e-1846.4617.6 kDa class I heat shock protein OS=Helianthus ... [more]
HSP11_SOYBN7.134e-1847.3717.3 kDa class I heat shock protein OS=Glycine max... [more]
HS17B_ARATH7.134e-1844.4417.6 kDa class I heat shock protein 2 OS=Arabidops... [more]
HS178_ARATH2.076e-1744.9017.8 kDa class I heat shock protein OS=Arabidopsis... [more]
HS17A_ARATH2.711e-1746.3917.6 kDa class I heat shock protein 1 OS=Arabidops... [more]
HS166_ORYSJ7.887e-1750.5516.6 kDa heat shock protein OS=Oryza sativa subsp.... [more]
HS219_ORYSJ6.677e-1643.6921.9 kDa heat shock protein OS=Oryza sativa subsp.... [more]
HS174_ARATH8.720e-1643.1617.4 kDa class III heat shock protein OS=Arabidops... [more]

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Properties
Property NameValue
Genbank descriptionFLAVEDO0003_IIIaR_A02 Flavedo Mature Citrus sinensis cDNA clone FLAVEDO0003_IIIaR_A02 3, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB304915 ID=CB304915; Name=CB304915; organism=Citrus sinensis; type=EST; length=701bp
TTTTTTTTTTTTTTTTTTTTTTTTTTAGATAAAAGCTGGATTTTTCATTA
ATTATAACAACTAGATGTCTACCAGTAGACGGCCGTCAGCTTCCCCTCCG
GAATGCCAAAGACCGAACCACAAACCAGTGAACAGAGAGAATTTTGGACA
AAAAAGGGAAGAAAGAGAAAAAAAGAAAAAGGAAGAGGAGATTACAGAGG
CATAGTACCGTCAAACCCACAAGCAAAATTATTCATAACAACCGGCAAAT
AGAAAAGACATCAGCACCCACAATCCAGCATATTCTCTCTCTCAAAGTTC
AAGCAATCTTAACCTCAATGGTCTTGGGCTTCTTGGGCTCCGGCGGTGGC
AGCTTATCGACAGTCACAGTCAACACTCCGTCCTGACACACCGCCGAAAT
GGCTTCAACGTTAGCATTCTCAGGCAGCACAAATTTCCTCATAAACTTGC
CAACCCTCCTCTCCATTCTCACATACTTGGCCCCATCTTTCTCCTCCTCC
CGCTTCCTCTCACCGCTTATGGGCAGCACATTGTCGTCCTCCACTTGGAC
CTTGATGTCCCCCGACTTCAGTCCGGGCATATCGACGATGAACACGTACG
AGTTCGGATACTCCTTCACGTCCGCAGGCGTCGCCGCCATTGCCTTGGCG
TCTCTCACGTACGTGCGCGTCGGCGCGTTGGATGACTTGTTGTCAGCGGA
G
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