CB322103

Overview
NameCB322103
Unique NameCB322103
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length591
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig11760 contig Csv1_Contig11760:910..1500. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS_BEII9 (1-deoxy-D-xylulose-5-phosphate synthase OS=Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) GN=dxs PE=3 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 9.265e-68
Identity = 126/195 (64.62%), Postives = 154/195 (78.97%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            LA+EG +PFCA+YS+FLQRGYDQVVHDV +Q LPVRFA+DRAGLVGADG TH G+FD++++  LPNMV+MA +DEAEL+HMVATAA  D+ P  FR+PRG G+G  LP    G  L+IG+GRI+ EG  VA+L  G+ + +   AA  L S  ISVTVADARF KPLD DL+R+LA  HE+LIT+EEGSVGGFGS
Sbjct:  382 LATEGYRPFCALYSTFLQRGYDQVVHDVAIQNLPVRFAIDRAGLVGADGATHAGSFDVSYLGILPNMVIMAAADEAELVHMVATAAAYDEGPIAFRYPRGEGVGIELP--QIGEILDIGRGRIVREGTTVALLSLGTRLAEASKAAEQLASYGISVTVADARFAKPLDLDLVRRLAKNHEVLITIEEGSVGGFGS 574
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS_BARBK (1-deoxy-D-xylulose-5-phosphate synthase OS=Bartonella bacilliformis (strain ATCC 35685 / KC583) GN=dxs PE=3 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 9.265e-68
Identity = 124/195 (63.59%), Postives = 154/195 (78.97%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            +A EG KPF AIYS+FLQR YDQ++HDV +QKLPVRFA+DRAG VGADG TH G+FDI F++ LP  VVMAPSDE ELMHMV TAAV D  P  FR+PRG GIG  LP   +G  LEIGKGR+L EG RVA++ +G+ + + ++AA+ L ++ IS TVADARF KPLD DL+R+LA EHE+ IT+EEG++GGFG+
Sbjct:  381 IACEGYKPFVAIYSTFLQRAYDQIIHDVSIQKLPVRFAIDRAGFVGADGATHAGSFDIVFLATLPEFVVMAPSDEVELMHMVRTAAVYDQGPISFRYPRGEGIGMDLP--QRGEVLEIGKGRVLREGSRVALVCFGTQLSEVLVAADELAAEGISTTVADARFAKPLDKDLMRRLAREHEVFITIEEGAIGGFGA 573
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS_MESSB (1-deoxy-D-xylulose-5-phosphate synthase OS=Mesorhizobium sp. (strain BNC1) GN=dxs PE=3 SV=1)

HSP 1 Score: 255.758 bits (652), Expect = 1.210e-67
Identity = 124/195 (63.59%), Postives = 154/195 (78.97%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            LA+EG +PF AIYS+FLQR YDQVVHDV +Q LPVRFA+DRAG VGADG THCGAFD+ +++ LPNMVVMA +DEAEL HMV TAA  D+ P  FR+PRG G G  LP   +G  L+IGKGRI+ EG ++A+L  G+ +  C++AA  L++  +S TVADARF KPLD DLIR+LA EHE+L+TVEEGS+GGFG+
Sbjct:  394 LATEGYRPFAAIYSTFLQRAYDQVVHDVAIQNLPVRFAIDRAGFVGADGATHCGAFDVAYLATLPNMVVMAAADEAELKHMVRTAADHDEGPIAFRYPRGEGTGVPLP--QRGEILKIGKGRIVREGSKIALLSLGTRLADCMIAAEELEAAGLSTTVADARFAKPLDADLIRRLAREHEVLVTVEEGSIGGFGA 586
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS_BARHE (1-deoxy-D-xylulose-5-phosphate synthase OS=Bartonella henselae GN=dxs PE=3 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 1.580e-67
Identity = 122/195 (62.56%), Postives = 155/195 (79.49%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            +A EG KPF AIYS+FLQR YDQ++HDV +QKLPVRFA+DRAG VGADG TH G+FDI F++ LP  VVMAPSDE ELMHMV TAA  D  P  FR+PRG GIG  LP   +G  LEIGKGRIL EG+R+A++ +G+ + + ++AA+ L ++ +S TVADARF KPLD DL+R+LA EHE+L+T+EEG++GGFG+
Sbjct:  381 MACEGYKPFVAIYSTFLQRAYDQIIHDVSIQKLPVRFAIDRAGFVGADGATHAGSFDIVFLTTLPEFVVMAPSDEVELMHMVRTAAAYDQGPISFRYPRGEGIGMDLP--QRGEILEIGKGRILHEGNRIALVCFGTRISEVLVAADELSAEGLSTTVADARFAKPLDKDLMRRLAREHEVLVTIEEGAIGGFGA 573
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS2_RHOS4 (1-deoxy-D-xylulose-5-phosphate synthase 2 OS=Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) GN=dxs2 PE=3 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 1.580e-67
Identity = 123/195 (63.08%), Postives = 158/195 (81.03%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            LA+ G++PFCAIYS+FLQRGYDQ+VHDV +Q+LPVRFA+DRAGLVGADG TH G+FD+ F+S LP +VVMA +DEAEL+HMVATAA  D+ P  FR+PRG+G+G  +P   KG PL+IG+GR++ EG R+A+L +G+ + +  +AA  L ++ IS TVADARF KPLD DLI QLA  HE LIT+EEG++GGFGS
Sbjct:  381 LAAGGMRPFCAIYSTFLQRGYDQIVHDVAIQRLPVRFAIDRAGLVGADGATHAGSFDVAFLSNLPGIVVMAAADEAELVHMVATAAAHDEGPIAFRYPRGDGVGVEMPV--KGVPLQIGRGRVVSEGTRIALLSFGTRLAEVQVAAEALAARGISPTVADARFAKPLDRDLILQLAAHHEALITIEEGAIGGFGS 573
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS_BRASO (1-deoxy-D-xylulose-5-phosphate synthase OS=Bradyrhizobium sp. (strain ORS278) GN=dxs PE=3 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 2.064e-67
Identity = 122/195 (62.56%), Postives = 154/195 (78.97%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            LA+EG KPFCAIYS+FLQRGYDQ+VHDV +Q LPVRFA+DRAGLVGADG TH G+FD  ++ CLPNMV+MA SDEAEL+HMVAT   IDDRPS  R+PRG G G  +P    G  L IGKGR++ +G +VA+L +G+ + +C  AA+ L +  +S ++ADARF KPLD +L+ +LA +HEILIT+EEGS+GGFGS
Sbjct:  382 LAAEGYKPFCAIYSTFLQRGYDQIVHDVCIQSLPVRFAIDRAGLVGADGATHAGSFDNAYLGCLPNMVIMAASDEAELVHMVATQVAIDDRPSSVRYPRGEGRGVEMP--EVGVALPIGKGRMIRQGKQVALLSFGTRLAECEKAADELAAHGLSASIADARFMKPLDEELVLKLARDHEILITIEEGSIGGFGS 574
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS_RHOCS (1-deoxy-D-xylulose-5-phosphate synthase OS=Rhodospirillum centenum (strain ATCC 51521 / SW) GN=dxs PE=3 SV=1)

HSP 1 Score: 254.218 bits (648), Expect = 3.521e-67
Identity = 123/195 (63.08%), Postives = 156/195 (80.00%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            LA+EG+KPFCAIYS+FLQR YDQVVHDV +Q LPVRFA+DRAGLVG+DG TH G+FD+ ++ C+PN+V+MA +DEAEL HMVATAA  D  P   R+PRG G+G  +P   +G  LEIGKGRI+ EG +VAIL  G+ +++ +LAA  L ++ +S TVADARF KP+D  LIR+LA +HE+LITVEEGS+GGFGS
Sbjct:  382 LAAEGLKPFCAIYSTFLQRAYDQVVHDVCIQNLPVRFAIDRAGLVGSDGCTHAGSFDVAYLGCVPNIVIMAAADEAELKHMVATAAAYDHGPIAVRYPRGEGVGLEMP--ERGQVLEIGKGRIVKEGTKVAILSLGTRLKEALLAAEDLNARGLSTTVADARFAKPIDEALIRRLAQDHEVLITVEEGSIGGFGS 574
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS_CAUCR (1-deoxy-D-xylulose-5-phosphate synthase OS=Caulobacter crescentus GN=dxs PE=3 SV=1)

HSP 1 Score: 254.218 bits (648), Expect = 3.521e-67
Identity = 126/195 (64.62%), Postives = 155/195 (79.49%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            +A++G+KPF AIYS+FLQRGYDQVVHDV +Q LPVRFAMDRAGLVGADGPTH G+FDI FM  LP MV+MA +DE EL  MVATAA IDDRPS FR+PRG G+G  +P      PLEIGKGRI+ EG  VAI+ +G+ + + + AA++L ++ +S TV DARF KPLD DL+ +LA EHE +ITVEEGS+GGFG+
Sbjct:  379 MAADGMKPFAAIYSTFLQRGYDQVVHDVAIQGLPVRFAMDRAGLVGADGPTHAGSFDIGFMGALPGMVLMAAADEVELARMVATAAEIDDRPSAFRYPRGEGLGLDMPA--IAEPLEIGKGRIVREGTSVAIVSFGTRLSESLKAADLLAARGLSATVCDARFAKPLDLDLLLRLAREHEAIITVEEGSMGGFGA 571
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS_CAUCN (1-deoxy-D-xylulose-5-phosphate synthase OS=Caulobacter crescentus (strain NA1000 / CB15N) GN=dxs PE=3 SV=1)

HSP 1 Score: 254.218 bits (648), Expect = 3.521e-67
Identity = 126/195 (64.62%), Postives = 155/195 (79.49%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            +A++G+KPF AIYS+FLQRGYDQVVHDV +Q LPVRFAMDRAGLVGADGPTH G+FDI FM  LP MV+MA +DE EL  MVATAA IDDRPS FR+PRG G+G  +P      PLEIGKGRI+ EG  VAI+ +G+ + + + AA++L ++ +S TV DARF KPLD DL+ +LA EHE +ITVEEGS+GGFG+
Sbjct:  379 MAADGMKPFAAIYSTFLQRGYDQVVHDVAIQGLPVRFAMDRAGLVGADGPTHAGSFDIGFMGALPGMVLMAAADEVELARMVATAAEIDDRPSAFRYPRGEGLGLDMPA--IAEPLEIGKGRIVREGTSVAIVSFGTRLSESLKAADLLAARGLSATVCDARFAKPLDLDLLLRLAREHEAIITVEEGSMGGFGA 571
[Close]
BLAST of CB322103 vs. ExPASy Swiss-Prot
Match: DXS_BART1 (1-deoxy-D-xylulose-5-phosphate synthase OS=Bartonella tribocorum (strain CIP 105476 / IBS 506) GN=dxs PE=3 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 7.844e-67
Identity = 122/195 (62.56%), Postives = 154/195 (78.97%), Query Frame = 1 
Query:    1 LASEGVKPFCAIYSSFLQRGYDQVVHDVDLQKLPVRFAMDRAGLVGADGPTHCGAFDITFMSCLPNMVVMAPSDEAELMHMVATAAVIDDRPSCFRFPRGNGIGAVLPPNNKGTPLEIGKGRILMEGDRVAILGYGSVVQQCVLAANMLKSQDISVTVADARFCKPLDTDLIRQLANEHEILITVEEGSVGGFGS 585
            LA EG KPF AIYS+FLQR YDQ++HDV +QKLPVRFA+DRAG VGADG TH G+FDI F++ LP  VVMAPSDE ELMHMV TAA  D  P  FR+PRG GIG  LP   +G  LEIGKGR+L EG+R+A++ +G+ + + + AA+ L ++ +S TVADARF KPLD DL+R+LA EHE+L+T+EEG++GGFG+
Sbjct:  381 LACEGYKPFVAIYSTFLQRAYDQIIHDVSIQKLPVRFAIDRAGFVGADGATHAGSFDIVFLATLPEFVVMAPSDELELMHMVRTAAAYDQGPISFRYPRGEGIGIDLP--QRGEILEIGKGRVLREGNRIALVCFGTRMSEVLRAADRLGAKGLSTTVADARFAKPLDKDLMRRLAREHEVLVTIEEGAIGGFGA 573
The following BLAST results are available for this feature:
BLAST of CB322103 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
DXS_BEII99.265e-6864.621-deoxy-D-xylulose-5-phosphate synthase OS=Beijeri... [more]
DXS_BARBK9.265e-6863.591-deoxy-D-xylulose-5-phosphate synthase OS=Bartone... [more]
DXS_MESSB1.210e-6763.591-deoxy-D-xylulose-5-phosphate synthase OS=Mesorhi... [more]
DXS_BARHE1.580e-6762.561-deoxy-D-xylulose-5-phosphate synthase OS=Bartone... [more]
DXS2_RHOS41.580e-6763.081-deoxy-D-xylulose-5-phosphate synthase 2 OS=Rhodo... [more]
DXS_BRASO2.064e-6762.561-deoxy-D-xylulose-5-phosphate synthase OS=Bradyrh... [more]
DXS_RHOCS3.521e-6763.081-deoxy-D-xylulose-5-phosphate synthase OS=Rhodosp... [more]
DXS_CAUCR3.521e-6764.621-deoxy-D-xylulose-5-phosphate synthase OS=Cauloba... [more]
DXS_CAUCN3.521e-6764.621-deoxy-D-xylulose-5-phosphate synthase OS=Cauloba... [more]
DXS_BART17.844e-6762.561-deoxy-D-xylulose-5-phosphate synthase OS=Bartone... [more]

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Properties
Property NameValue
Genbank descriptionEST0212 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB063 similar to 1-deoxyxylulose-5-phosphate synthase (acc# CAA09804), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322103 ID=CB322103; Name=CB322103; organism=Citrus sinensis; type=EST; length=591bp
TTAGCCTCAGAGGGAGTCAAGCCATTTTGTGCCATCTACTCATCATTTCT
GCAAAGAGGATATGATCAGGTTGTGCATGATGTAGACCTTCAAAAATTAC
CAGTCCGCTTTGCAATGGATCGGGCTGGTTTGGTTGGTGCGGATGGACCT
ACTCATTGTGGAGCATTTGACATCACATTCATGTCTTGCTTGCCCAACAT
GGTGGTCATGGCTCCATCTGATGAAGCTGAGCTAATGCACATGGTCGCTA
CAGCAGCAGTTATAGATGACAGGCCCAGCTGTTTCAGATTTCCAAGGGGC
AACGGAATTGGAGCAGTTCTCCCACCTAATAACAAAGGAACTCCACTTGA
GATTGGGAAGGGAAGAATCCTAATGGAAGGCGATAGAGTGGCAATTTTGG
GCTATGGTTCTGTAGTCCAACAATGTGTGCTAGCTGCGAACATGCTTAAG
AGCCAAGACATTTCCGTGACAGTGGCTGATGCAAGATTTTGCAAACCTTT
GGATACAGACCTCATTAGACAGTTGGCCAATGAGCATGAAATCCTAATTA
CTGTGGAAGAGGGTTCTGTGGGAGGCTTTGGCTCTCTGTAT
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