CB322179

Overview
NameCB322179
Unique NameCB322179
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length425
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_SHEB5 (Diaminopimelate epimerase OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=dapF PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.467e-11
Identity = 34/108 (31.48%), Postives = 53/108 (49.07%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            V   ++AEIG     H  FP   N  F++++    +K+RV+ER                    ++G   ++  VDLPGG L I W+ E   ++MTGPA+ V+ G + L
Sbjct:  168 VASASVAEIGAMLTKHERFPRGVNVGFMQVVDSGHIKLRVYERGAAETLACGTGACAAVVVGQVQGKLGQQVRVDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL 275
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_SHEB2 (Diaminopimelate epimerase OS=Shewanella baltica (strain OS223) GN=dapF PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.467e-11
Identity = 34/108 (31.48%), Postives = 53/108 (49.07%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            V   ++AEIG     H  FP   N  F++++    +K+RV+ER                    ++G   ++  VDLPGG L I W+ E   ++MTGPA+ V+ G + L
Sbjct:  168 VASASVAEIGAMLTKHERFPRGVNVGFMQVVDSGHIKLRVYERGAAETLACGTGACAAVVVGQVQGKLGQQVRVDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL 275
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_HALHL (Diaminopimelate epimerase OS=Halorhodospira halophila (strain DSM 244 / SL1) GN=dapF PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.467e-11
Identity = 38/113 (33.63%), Postives = 55/113 (48.67%), Query Frame = -3 
Query:   79 LKVDKLN---LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 408
            L+VD +    +AE+GP+ E H  FP R N  F+E+ +  ++++RV+ER                    L    +   TV+L GG L I W      V+MTGPA  VF G + L
Sbjct:  162 LRVDDMTGAPVAELGPEIERHPRFPRRVNVGFMEVCTRDRIRLRVFERGVGETPACGTGACAAVVAGRLRDWLDAPVTVELTGGVLVIHWLGPGRSVWMTGPARTVFRGEIEL 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_VIBCH (Diaminopimelate epimerase OS=Vibrio cholerae GN=dapF PE=3 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 5.834e-11
Identity = 31/98 (31.63%), Postives = 50/98 (51.02%), Query Frame = -3 
Query:   85 IGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSV 378
            +GP  E H  FP R N  F++++S  ++ +RV+ER                   +L+G  + +  V LPGG L+I W+     +YMTGPA  ++ G +
Sbjct:  177 LGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAAVAVGILQGLLDEQVRVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQI 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_VIBC3 (Diaminopimelate epimerase OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=dapF PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.834e-11
Identity = 31/98 (31.63%), Postives = 50/98 (51.02%), Query Frame = -3 
Query:   85 IGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSV 378
            +GP  E H  FP R N  F++++S  ++ +RV+ER                   +L+G  + +  V LPGG L+I W+     +YMTGPA  ++ G +
Sbjct:  177 LGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAAVAVGILQGLLDEQVRVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQI 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_VARPS (Diaminopimelate epimerase OS=Variovorax paradoxus (strain S110) GN=dapF PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.834e-11
Identity = 38/106 (35.85%), Postives = 50/106 (47.17%), Query Frame = -3 
Query:   85 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSV 402
            VD   +A  GP  EHH  F  R N  F++++  S +K+RV+ER                   +  G  ERR  V   GG L I W+ E + V MTGPA  VF G +
Sbjct:  183 VDTAPVARQGPLIEHHPRFAQRVNAGFMQVVDRSHIKLRVFERGAGETLACGTGACAAVVAGIRLGLLERRVDVQTHGGVLTIGWEGEGHPVLMTGPATTVFEGDI 288
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_VIBHB (Diaminopimelate epimerase OS=Vibrio harveyi (strain ATCC BAA-1116 / BB120) GN=dapF PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.619e-11
Identity = 33/106 (31.13%), Postives = 52/106 (49.06%), Query Frame = -3 
Query:   85 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSV 402
            VD   +  +GP  E H  FP R N  F+++++ + +++RV+ER                   +L+G  +    V LPGG L I W+     ++MTGPA  VF G +
Sbjct:  169 VDTAEVETLGPLLESHERFPERVNAGFMQVVNRNHIRLRVYERGAGETQACGSGACGAVAVGILQGLLDENVKVSLPGGDLRISWQGPGKPLFMTGPATHVFDGQL 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_SHIB3 (Diaminopimelate epimerase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=dapF PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.619e-11
Identity = 35/108 (32.41%), Postives = 52/108 (48.15%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            VD   +  +GP  E H  FP R N  F++++    +++RV+ER                   + +G       V+LPGG LDI WK   + +YMTGPA  V+ G + L
Sbjct:  167 VDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_ESCF3 (Diaminopimelate epimerase OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=dapF PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.619e-11
Identity = 35/108 (32.41%), Postives = 52/108 (48.15%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            VD   +  +GP  E H  FP R N  F++++    +++RV+ER                   + +G       V+LPGG LDI WK   + +YMTGPA  V+ G + L
Sbjct:  167 VDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_ERWT9 (Diaminopimelate epimerase OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=dapF PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.619e-11
Identity = 37/113 (32.74%), Postives = 54/113 (47.79%), Query Frame = -3 
Query:   79 LKVDKLNLAEI---GPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 408
            L+VD +  A +   GP  E H  FP R N  F+E++    +++RV+ER                   + +     +  VDLPGG L I WK   + +YMTGPA  V+ G + L
Sbjct:  162 LQVDNVKTAAVETLGPVLESHERFPERVNVGFMEVVHREHIRLRVYERGAGETQACGSGACAAVAVGIQQASLAEKVRVDLPGGSLHIAWKGPGHPLYMTGPATHVYDGFIHL 274
The following BLAST results are available for this feature:
BLAST of CB322179 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 204
Match NameE-valueIdentityDescription
DAPF_SHEB54.467e-1131.48Diaminopimelate epimerase OS=Shewanella baltica (s... [more]
DAPF_SHEB24.467e-1131.48Diaminopimelate epimerase OS=Shewanella baltica (s... [more]
DAPF_HALHL4.467e-1133.63Diaminopimelate epimerase OS=Halorhodospira haloph... [more]
DAPF_VIBCH5.834e-1131.63Diaminopimelate epimerase OS=Vibrio cholerae GN=da... [more]
DAPF_VIBC35.834e-1131.63Diaminopimelate epimerase OS=Vibrio cholerae serot... [more]
DAPF_VARPS5.834e-1135.85Diaminopimelate epimerase OS=Variovorax paradoxus ... [more]
DAPF_VIBHB7.619e-1131.13Diaminopimelate epimerase OS=Vibrio harveyi (strai... [more]
DAPF_SHIB37.619e-1132.41Diaminopimelate epimerase OS=Shigella boydii serot... [more]
DAPF_ESCF37.619e-1132.41Diaminopimelate epimerase OS=Escherichia fergusoni... [more]
DAPF_ERWT97.619e-1132.74Diaminopimelate epimerase OS=Erwinia tasmaniensis ... [more]

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Properties
Property NameValue
Genbank descriptionEST0300 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB226 similar to diaminopimelate epimerase (acc# NP_190926), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322179 ID=CB322179; Name=CB322179; organism=Citrus sinensis; type=EST; length=425bp
ACATCTTTCTAGCTAACAATGCGCGTCTGCAAAATTCAGGTAATCTGAGA
CAAGGAAGTTCTCGACAATAAATTAATTTAGCAGGACCGATCCATAAAAC
ACTACTTCTGCCGGGCCAGTCATATATACATGGTTGTCTTCCTCCTTCCA
CTCAATATCCAATGGCCCTCCAGGTAGATCAACAGTGCATCTTCTCTCAG
CATGACCCTCAAGAACTGCTGCGACCACAACTGCACAAGCACCAGTCCCA
CATGCCAGGGTTGCTCCAGCCCCACGTTCCCAAACACGCATCTTCAGTTG
TGAAGGAGAAATAATCTCCACAAATTCTGTATTAGTTCGTGCTGGGAACA
CAGAATGATGCTCAAATTTAGGACCAATTTCAGCCAAGTTTAGTTTATCA
ACCTTCAAATTCTGCCCTTCTTTTG
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