CB322212

Overview
NameCB322212
Unique NameCB322212
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length552
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_YEAST (Thiazole biosynthetic enzyme, mitochondrial OS=Saccharomyces cerevisiae GN=THI4 PE=1 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 5.898e-39
Identity = 91/189 (48.15%), Postives = 113/189 (59.79%), Query Frame = 3
Query:    3 DEQDNYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGG------RVGGVVTNWALVSMNHDTQSCMDPNVMEAK---------------IVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREI--VPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALK 500
            +++ +YVV+KHAALF ST++SK+L  PNVKLFNA   EDL+ +         V GVVTNW LV+  H TQ CMDPNV+E                 +++S+ GHDGPFGA   KR+  I     + GMK LDMN AE  +V  +     V  M   GMEVAE+DG  RMGPTFGAM +SG  AA   LK
Sbjct:  134 EDEGDYVVVKHAALFISTVLSKVLQLPNVKLFNATCVEDLVTRPPTEKGEVTVAGVVTNWTLVTQAHGTQCCMDPNVIELAGYKNDGTRDLSQKHGVILSTTGHDGPFGAFCAKRIVDIDQNQKLGGMKGLDMNHAEHDVVIHSGAYAGVDNMYFAGMEVAELDGLNRMGPTFGAMALSGVHAAEQILK 322          
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_PYRFU (Putative thiazole biosynthetic enzyme OS=Pyrococcus furiosus GN=PF1530 PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.276e-25
Identity = 66/157 (42.04%), Postives = 89/157 (56.69%), Query Frame = 3
Query:   39 ALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKALG 509
            A+  +T ++    +  VK+FN V  EDL+VK  RV G+V NW  V M   T   +DP  +EAK V+ S GH    GA   + L   G+I+ +PG  A+     E   V  T+E+ PG+ VTGM    + GAPRMGP FG M +SG+KAA   L+ LG
Sbjct:  102 AIEVATTIASKTVKAGVKIFNMVEVEDLVVKDDRVSGIVINWTPVKM---TGLHVDPLTVEAKYVIDSTGH----GAQVTQFLLKRGLIEKIPGEGAMWAEMGEKLTVENTKEVFPGLYVTGMAANAVSGAPRMGPIFGGMFLSGRKAAMEILQKLG 251          
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_PYRKO (Putative thiazole biosynthetic enzyme OS=Pyrococcus kodakaraensis GN=TK0434 PE=3 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.711e-25
Identity = 68/154 (44.16%), Postives = 90/154 (58.44%), Query Frame = 3
Query:   51 STIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKALGQ 512
            ST+ SK + +   K+FN V  EDL+VK GRV G+V NW  V M   T   +DP  +EAK VV S GH    GA   + L   G+I ++PG   +     E+  V  TRE+ PG+  TGM    + GAPRMGP FG M++SG+KAA   L+ LG+
Sbjct:  106 STLASKAV-KAGAKIFNMVEVEDLVVKDGRVSGLVINWTPVMM---TGLHVDPLTVEAKFVVDSTGH----GAQISQHLLKRGLIKAIPGEGPMWAEKGEELTVEHTREVFPGLYATGMAANALAGAPRMGPIFGGMLLSGRKAALEILQKLGK 251          
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_PYRHO (Putative thiazole biosynthetic enzyme OS=Pyrococcus horikoshii GN=PH1357 PE=3 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 6.330e-25
Identity = 64/156 (41.03%), Postives = 87/156 (55.77%), Query Frame = 3
Query:   39 ALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKAL 506
            A+  +T ++  + +  VK+FN +  EDL++K  RV G+V NW  V M       +DP  +EAK V+ S GH        VKR    G++  +PG  A+     E   V+ TRE+ PG+ VTGM    I GAPRMGP FG M +SG+KAA   LK L
Sbjct:  102 AIEVATTIASKVVKSGVKIFNMIEVEDLVIKDNRVSGIVINWTPVLM---AGLHVDPLTIEAKYVIDSTGHGAQVAQFLVKR----GLLKEIPGEGAMWAEQGEKLTVKNTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGRKAAQEILKKL 250          
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_PYRAB (Putative thiazole biosynthetic enzyme OS=Pyrococcus abyssi GN=PYRAB07910 PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 6.330e-25
Identity = 66/156 (42.31%), Postives = 88/156 (56.41%), Query Frame = 3
Query:   39 ALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKAL 506
            A+  +T ++    +  VK+FN +  EDL+VK  RV G+V NW  V M   T   +DP  +EAK V+ S GH    GA   + L   G+I+ +PG  A+     E   V  TRE+ PG+ VTGM    I GAPRMGP FG M +SG+KAA   L+ L
Sbjct:  102 AIEVATTIASKTVKAGVKIFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQVAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGKKAAQEILEKL 250          
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_AERPE (Putative thiazole biosynthetic enzyme OS=Aeropyrum pernix GN=APE_2149.1 PE=3 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 2.941e-22
Identity = 60/154 (38.96%), Postives = 92/154 (59.74%), Query Frame = 3
Query:   57 IMSKLLARP---NVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKALG 509
            + +KL AR      ++ N    +D+IV+  RV GVV NW+ V       +C+DP  + A+ VV + GHD    A   ++L   GM+++   +  + +  +ED +V  T E+ PG++V G+ VAE+ G PRMGPTFGAM++SG+KAA L  + LG
Sbjct:  109 LAAKLAARALEAGAEVLNLTMLDDVIVENSRVAGVVVNWSPVQGLPRQITCVDPVGLRAEYVVDATGHD----AVVTRKLAERGMVEASK-LGPMWVERSEDLVVEKTGEVYPGLVVAGIAVAEVYGLPRMGPTFGAMLLSGEKAAALIGEKLG 257          
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_HALWD (Putative thiazole biosynthetic enzyme OS=Haloquadratum walsbyi (strain DSM 16790) GN=HQ1276A PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.510e-18
Identity = 53/148 (35.81%), Postives = 80/148 (54.05%), Query Frame = 3
Query:  138 RVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHD----------GPFGATGVKRLK--SIGMIDSV-----------PGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKALGQ 512
            RVGG+V NW  V       +C+DP  +E+ +VV S GHD          G   A G++     + GM ++            PG  ++ ++ +ED +V  T  + PG++ TGM VA ++G PRMGPTFGAM++SG++ A   L  LG+
Sbjct:  145 RVGGIVLNWTPVHALPRELTCVDPVAVESDLVVDSTGHDAVVISKLSERGVLNAPGIEHANEHNTGMDNTADDEYGAPGHDSPGHDSMWVSRSEDQVVEHTGVVHPGVVATGMAVATVEGLPRMGPTFGAMLLSGKRGAQACLNELGR 292          
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_METTP (Putative thiazole biosynthetic enzyme OS=Methanosaeta thermophila (strain DSM 6194 / PT) GN=Mthe_1121 PE=3 SV=2)

HSP 1 Score: 90.8929 bits (224), Expect = 4.394e-18
Identity = 52/143 (36.36%), Postives = 79/143 (55.24%), Query Frame = 3
Query:   90 KLFNAVAAEDLIVK-GGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDS-VPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKALGQ 512
            +L N V+ ED++++ G R+ G+V NW    M       +DP  + A++V+   GHD        K++    + +S V G K +     E  +V  TRE+ PG+IV GM    +   PRMGP FG M++SG+KAA +AL+ L Q
Sbjct:  120 ELINLVSVEDVMIREGDRIVGLVINWTAADM---AGIHVDPLAIRARVVIDGTGHDAAVCRVVQKKIPGAIVGESGVIGEKPMWAALGEKIVVDATREVYPGLIVAGMAATTVAAGPRMGPIFGGMLLSGEKAASIALEKLAQ 259          
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_HALMA (Putative thiazole biosynthetic enzyme OS=Haloarcula marismortui GN=rrnAC1782 PE=3 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 5.739e-18
Identity = 59/164 (35.98%), Postives = 84/164 (51.22%), Query Frame = 3
Query:   90 KLFNAVAAEDLIVKGG-RVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMI------DSVPGMKALDMNT------------------AEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKAL 506
            K+ N     D++++   RVGG+V NW  V       +C+DP  +EA +V+ + GHD    A  VK+L   G++      D   GM   D +T                  +EDA+V  T     G++VTGM  A   G PRMGPTFGAM++SG++AA +AL  L
Sbjct:  132 KMQNMTEFTDIVIREDHRVGGIVMNWTPVHALPREITCVDPIAVEADLVIDATGHD----AMAVKKLDERGVLNAPGLEDEASGMDQTDDDTYGAPGHDSPGHDSMWVGESEDAVVEHTGLAHDGLVVTGMATATTYGLPRMGPTFGAMLVSGKRAAQVALDEL 291          
BLAST of CB322212 vs. ExPASy Swiss-Prot
Match: THI4_NATPD (Putative thiazole biosynthetic enzyme OS=Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) GN=NP5174A PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.279e-17
Identity = 57/168 (33.93%), Postives = 85/168 (50.60%), Query Frame = 3
Query:   90 KLFNAVAAEDLIVKGG-RVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSV---------------------------PGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKALG 509
            K+ N     D++++   RVGG+V NW  V       +C+DP  +EA +V+ + GHD    A  V +L+  G++++                            PG  ++ +  +EDA+V  T     G+IVTGM  A   G PRMGPTFGAM++SG++AA  AL  LG
Sbjct:  129 KMQNMTEFTDIVIREDHRVGGIVMNWTPVHALPREITCVDPIAVEADLVIDATGHD----AVAVSKLQERGVLNAPGIEHADEHNTGMDQTGDDSYGAPGHDSPGHDSMWVGQSEDAVVEHTGLAHEGLIVTGMATATTYGLPRMGPTFGAMLLSGKRAAQAALDELG 292          
The following BLAST results are available for this feature:
BLAST of CB322212 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 55
Match NameE-valueIdentityDescription
THI4_YEAST5.898e-3948.15Thiazole biosynthetic enzyme, mitochondrial OS=Sac... [more]
THI4_PYRFU1.276e-2542.04Putative thiazole biosynthetic enzyme OS=Pyrococcu... [more]
THI4_PYRKO3.711e-2544.16Putative thiazole biosynthetic enzyme OS=Pyrococcu... [more]
THI4_PYRHO6.330e-2541.03Putative thiazole biosynthetic enzyme OS=Pyrococcu... [more]
THI4_PYRAB6.330e-2542.31Putative thiazole biosynthetic enzyme OS=Pyrococcu... [more]
THI4_AERPE2.941e-2238.96Putative thiazole biosynthetic enzyme OS=Aeropyrum... [more]
THI4_HALWD1.510e-1835.81Putative thiazole biosynthetic enzyme OS=Haloquadr... [more]
THI4_METTP4.394e-1836.36Putative thiazole biosynthetic enzyme OS=Methanosa... [more]
THI4_HALMA5.739e-1835.98Putative thiazole biosynthetic enzyme OS=Haloarcul... [more]
THI4_NATPD1.279e-1733.93Putative thiazole biosynthetic enzyme OS=Natronomo... [more]

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Properties
Property NameValue
Genbank descriptionEST0336 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB352 similar to thiamin biosynthesis protein thi1 (acc# T10474), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322212 ID=CB322212; Name=CB322212; organism=Citrus sinensis; type=EST; length=552bp
ACGACGAGCAAGACAACTACGTCGTGATCAAGCACGCCGCGTTGTTCACT
TCAACAATCATGAGCAAGCTGTTGGCGAGGCCAAACGTGAAGTTGTTCAA
CGCTGTTGCAGCTGAGGATTTGATCGTCAAGGGAGGGAGAGTAGGTGGCG
TCGTCACAAACTGGGCCTTGGTGTCTATGAATCACGACACACAGTCTTGC
ATGGACCCCAATGTGATGGAGGCCAAGATTGTGGTCAGCTCGTGTGGCCA
TGACGGCCCTTTTGGGGCTACCGGTGTTAAGAGGCTTAAGAGTATTGGCA
TGATTGACTCCGTCCCCGGAATGAAGGCTCTCGATATGAACACTGCAGAG
GATGCTATCGTTAGGCTTACCAGGGAGATCGTGCCTGGCATGATTGTCAC
GGGCATGGAAGTCGCAGAGATCGACGGTGCCCCTAGAATGGGACCTACAT
TCGGGGCCATGATGATTTCAGGGCAGAAGGCAGCCCATTTGGCGTTGAAG
GCATTGGGGCAGCCGAATGCGATAGATGGGACATTCAGTGAAGTTACGGA
AG
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