CB322228

Overview
NameCB322228
Unique NameCB322228
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length633
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_CAMDR (Ubiquitin OS=Camelus dromedarius PE=3 SV=2)

HSP 1 Score: 145.976 bits (367), Expect = 1.548e-34
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 145.976 bits (367), Expect = 1.548e-34
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 522
            MQIFVKTLTGKTITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_BOVIN (Ubiquitin OS=Bos taurus GN=RPS27A PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.548e-34
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 145.976 bits (367), Expect = 1.548e-34
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 522
            MQIFVKTLTGKTITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_TETPY (Ubiquitin OS=Tetrahymena pyriformis GN=TUF11 PE=3 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.022e-34
Identity = 72/76 (94.74%), Postives = 76/76 (100.00%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTITL+VE+SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLDVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 145.591 bits (366), Expect = 2.022e-34
Identity = 72/76 (94.74%), Postives = 76/76 (100.00%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 522
            MQIFVKTLTGKTITL+VE+SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLDVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_ENCCU (Ubiquitin OS=Encephalitozoon cuniculi GN=ECU02_0740i PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.022e-34
Identity = 72/77 (93.51%), Postives = 76/77 (98.70%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 525
            MQIFVKTLTGKTITLEVE SD+I+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG+
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDSIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGY 77

HSP 2 Score: 144.436 bits (363), Expect = 4.504e-34
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTITLEVE SD+I+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDSIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_SHEEP (Ubiquitin OS=Ovis aries GN=UBA52 PE=3 SV=2)

HSP 1 Score: 145.206 bits (365), Expect = 2.641e-34
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLR+RGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRMRGG 76

HSP 2 Score: 145.206 bits (365), Expect = 2.641e-34
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 522
            MQIFVKTLTGKTITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLR+RGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRMRGG 76
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_EIMBO (Ubiquitin OS=Eimeria bovis PE=2 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 3.449e-34
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTITL+VE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLDVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 144.821 bits (364), Expect = 3.449e-34
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 522
            MQIFVKTLTGKTITL+VE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLDVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_TRYBB (Ubiquitin OS=Trypanosoma brucei brucei PE=3 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 4.504e-34
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTI LEVE+SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTLADYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLADYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 144.436 bits (363), Expect = 4.504e-34
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 522
            MQIFVKTLTGKTI LEVE+SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTLADYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLADYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_STRPU (Ubiquitin OS=Strongylocentrotus purpuratus PE=3 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 4.504e-34
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTITLEVE SD+I+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDSIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 144.436 bits (363), Expect = 4.504e-34
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 522
            MQIFVKTLTGKTITLEVE SD+I+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDSIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_DICDI (Ubiquitin OS=Dictyostelium discoideum GN=ubqA PE=1 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 4.504e-34
Identity = 72/76 (94.74%), Postives = 74/76 (97.37%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTITLEVE SD I+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEGSDNIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 144.436 bits (363), Expect = 4.504e-34
Identity = 72/76 (94.74%), Postives = 74/76 (97.37%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 522
            MQIFVKTLTGKTITLEVE SD I+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEGSDNIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CB322228 vs. ExPASy Swiss-Prot
Match: UBIQ_PHYIN (Ubiquitin OS=Phytophthora infestans PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.311e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 1 
Query:   67 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 294
            MQIFVKTLTGKTITL+VE SD+IDNVK KIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLDVEPSDSIDNVKQKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 142.895 bits (359), Expect = 1.311e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 1 
Query:  295 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 522
            MQIFVKTLTGKTITL+VE SD+IDNVK KIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLDVEPSDSIDNVKQKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76
The following BLAST results are available for this feature:
BLAST of CB322228 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
UBIQ_CAMDR1.548e-3496.05Ubiquitin OS=Camelus dromedarius PE=3 SV=2[more]
UBIQ_BOVIN1.548e-3496.05Ubiquitin OS=Bos taurus GN=RPS27A PE=1 SV=1[more]
UBIQ_TETPY2.022e-3494.74Ubiquitin OS=Tetrahymena pyriformis GN=TUF11 PE=3 ... [more]
UBIQ_ENCCU2.022e-3493.51Ubiquitin OS=Encephalitozoon cuniculi GN=ECU02_074... [more]
UBIQ_SHEEP2.641e-3494.74Ubiquitin OS=Ovis aries GN=UBA52 PE=3 SV=2[more]
UBIQ_EIMBO3.449e-3494.74Ubiquitin OS=Eimeria bovis PE=2 SV=1[more]
UBIQ_TRYBB4.504e-3494.74Ubiquitin OS=Trypanosoma brucei brucei PE=3 SV=1[more]
UBIQ_STRPU4.504e-3494.74Ubiquitin OS=Strongylocentrotus purpuratus PE=3 SV... [more]
UBIQ_DICDI4.504e-3494.74Ubiquitin OS=Dictyostelium discoideum GN=ubqA PE=1... [more]
UBIQ_PHYIN1.311e-3393.42Ubiquitin OS=Phytophthora infestans PE=1 SV=1[more]

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Properties
Property NameValue
Genbank descriptionEST0354 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB370 similar to pentameric polyubiquitin (acc# AAA34124), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322228 ID=CB322228; Name=CB322228; organism=Citrus sinensis; type=EST; length=633bp
ACTCTTGCTGACTACAACATCCAGAAGGAGTCAACCCTCCATTTGGTGCT
TCGTCTCCGTGGTGGCATGCAGATCTTTGTGAAAACCCTCACCGGAAAAA
CAATTACTCTTGAGGTTGAAAGTTCTGACACCATCGACAACGTGAAAGCA
AAAATTCAAGACAAGGAGGGTATCCCACCAGATCAGCAGAGGCTCATTTT
TGCTGGAAAGCAGCTTGAAGATGGAAGGACTCTTGCAGACTATAACATCC
AGAAAGAATCCACTCTTCACTTGGTTCTTCGCCTCCGTGGTGGTATGCAG
ATTTTTGTGAAGACACTCACTGGGAAAACCATCACATTGGAGGTTGAGAG
TTCGGACACAATTGACAATGTGAAGGCTAAGATCCAGGATAAGGAGGGGA
TTCCCCCAGACCAGCAGAGGTTGATCTTTGCTGGAAAACAACTTGAGGAT
GGAAGGACTCTTGCTGATTACAACATCCAGAAGGAGTCCACTCTGCATCT
TGTGCTCCGTCTCCGTGGAGGTTTCTGAGTTTGGTCATCTGGGTGTGTGT
GTGTCTTATTTGCTTCGGTTTGTCTTGGTTAATGTTTGCTTGCTATTGGT
GAAATGGCTTAAATAACTATTTGAAGTTTATTT
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