CB417303

Overview
NameCB417303
Unique NameCB417303
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length348
Libraries
Library NameType
Mature peel (flavedo) cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig376 contig Csv1_Contig376:1..348. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_BURP0 (Alanyl-tRNA synthetase OS=Burkholderia pseudomallei (strain 1106a) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 6.215e-26
Identity = 60/117 (51.28%), Postives = 76/117 (64.96%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK--FLPASR 347
            K +FLG      +    +RA   Q+ +RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK +AI + WELLT VY+LPAD+L+ T +  D+        EA DIW K   +PA R
Sbjct:   46 KDVFLGA-----ETRPYSRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWELLTSVYKLPADKLWVTVYHDDD--------EAYDIWAKEVGVPAER 149
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_BURMS (Alanyl-tRNA synthetase OS=Burkholderia mallei (strain SAVP1) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 6.215e-26
Identity = 60/117 (51.28%), Postives = 76/117 (64.96%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK--FLPASR 347
            K +FLG      +    +RA   Q+ +RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK +AI + WELLT VY+LPAD+L+ T +  D+        EA DIW K   +PA R
Sbjct:   46 KDVFLGA-----ETRPYSRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWELLTSVYKLPADKLWVTVYHDDD--------EAYDIWAKEVGVPAER 149
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_BURMA (Alanyl-tRNA synthetase OS=Burkholderia mallei GN=alaS PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 6.215e-26
Identity = 60/117 (51.28%), Postives = 76/117 (64.96%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK--FLPASR 347
            K +FLG      +    +RA   Q+ +RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK +AI + WELLT VY+LPAD+L+ T +  D+        EA DIW K   +PA R
Sbjct:   46 KDVFLGA-----ETRPYSRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWELLTSVYKLPADKLWVTVYHDDD--------EAYDIWAKEVGVPAER 149
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_BURM9 (Alanyl-tRNA synthetase OS=Burkholderia mallei (strain NCTC 10229) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 6.215e-26
Identity = 60/117 (51.28%), Postives = 76/117 (64.96%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK--FLPASR 347
            K +FLG      +    +RA   Q+ +RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK +AI + WELLT VY+LPAD+L+ T +  D+        EA DIW K   +PA R
Sbjct:   46 KDVFLGA-----ETRPYSRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWELLTSVYKLPADKLWVTVYHDDD--------EAYDIWAKEVGVPAER 149
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_BURM7 (Alanyl-tRNA synthetase OS=Burkholderia mallei (strain NCTC 10247) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 6.215e-26
Identity = 60/117 (51.28%), Postives = 76/117 (64.96%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK--FLPASR 347
            K +FLG      +    +RA   Q+ +RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK +AI + WELLT VY+LPAD+L+ T +  D+        EA DIW K   +PA R
Sbjct:   46 KDVFLGA-----ETRPYSRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWELLTSVYKLPADKLWVTVYHDDD--------EAYDIWAKEVGVPAER 149
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_BURCH (Alanyl-tRNA synthetase OS=Burkholderia cenocepacia (strain HI2424) GN=alaS PE=3 SV=2)

HSP 1 Score: 115.931 bits (289), Expect = 6.215e-26
Identity = 59/109 (54.13%), Postives = 75/109 (68.81%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK 329
            K +FLGT DP       +RA   Q+ +RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK++AI++ WELLT VY+LP D+L+ T +          D+EA DIW K
Sbjct:   46 KDVFLGT-DPRPY----SRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIKFAWELLTTVYQLPKDKLWVTVY--------QEDDEAYDIWAK 141
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_BURCC (Alanyl-tRNA synthetase OS=Burkholderia cenocepacia (strain MC0-3) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 6.215e-26
Identity = 59/109 (54.13%), Postives = 75/109 (68.81%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK 329
            K +FLGT DP       +RA   Q+ +RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK++AI++ WELLT VY+LP D+L+ T +          D+EA DIW K
Sbjct:   46 KDVFLGT-DPRPY----SRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIKFAWELLTTVYQLPKDKLWVTVY--------QEDDEAYDIWAK 141
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_SYNAS (Alanyl-tRNA synthetase OS=Syntrophus aciditrophicus (strain SB) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 62/117 (52.99%), Postives = 75/117 (64.10%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK--FLPASR 347
            K  FLG  D        +RA + QKC+RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK EAI W WE LT+V +LP +RL+ T F          D+EA  IWL+   +PA R
Sbjct:   48 KNAFLGLEDRG-----YSRAASCQKCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFKREAIAWAWEYLTEVIQLPKERLWVTVF--------QDDDEAYRIWLEEMRIPADR 151
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_METPP (Alanyl-tRNA synthetase OS=Methylibium petroleiphilum (strain PM1) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 57/100 (57.00%), Postives = 72/100 (72.00%), Query Frame = 3 
Query:   54 TRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK--FLPASR 347
            +RA   QKCIRAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK +AI + WELLT+V++LP D+L+ T +        A D+EA +IW K   +PA R
Sbjct:   58 SRATTAQKCIRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQDAIAYAWELLTEVFKLPKDKLWVTVY--------AEDDEAYEIWNKQVGVPAER 149
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_FRATW (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. tularensis (strain WY96-3418) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 57/111 (51.35%), Postives = 72/111 (64.86%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLKFL 335
            K +FLG      +    +RA   QKC+RAGGKHNDLD+VG    HHTFFEMLGN+SFGDYFK EAI + WE LTK  +LP ++L+ T +        A D+EA D+W K +
Sbjct:   47 KDVFLGI-----EKKDFSRAVTVQKCLRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKKEAISFAWEFLTKEIKLPVEKLWVTIY--------ASDDEAFDVWHKHI 144
The following BLAST results are available for this feature:
BLAST of CB417303 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
SYA_BURP06.215e-2651.28Alanyl-tRNA synthetase OS=Burkholderia pseudomalle... [more]
SYA_BURMS6.215e-2651.28Alanyl-tRNA synthetase OS=Burkholderia mallei (str... [more]
SYA_BURMA6.215e-2651.28Alanyl-tRNA synthetase OS=Burkholderia mallei GN=a... [more]
SYA_BURM96.215e-2651.28Alanyl-tRNA synthetase OS=Burkholderia mallei (str... [more]
SYA_BURM76.215e-2651.28Alanyl-tRNA synthetase OS=Burkholderia mallei (str... [more]
SYA_BURCH6.215e-2654.13Alanyl-tRNA synthetase OS=Burkholderia cenocepacia... [more]
SYA_BURCC6.215e-2654.13Alanyl-tRNA synthetase OS=Burkholderia cenocepacia... [more]
SYA_SYNAS8.118e-2652.99Alanyl-tRNA synthetase OS=Syntrophus aciditrophicu... [more]
SYA_METPP8.118e-2657.00Alanyl-tRNA synthetase OS=Methylibium petroleiphil... [more]
SYA_FRATW8.118e-2651.35Alanyl-tRNA synthetase OS=Francisella tularensis s... [more]

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Properties
Property NameValue
Genbank descriptionEST0379 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P024 similar to alanine tRNA ligase (acc# NP_175439), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB417303 ID=CB417303; Name=CB417303; organism=Citrus sinensis; type=EST; length=348bp
ACAAGCCGATTTTTCTTGGAACCGCGGATCCGAATACTCAATTGAGTAAG
CTGACGCGCGCGTTTAACACTCAGAAGTGTATACGCGCCGGTGGTAAGCA
TAATGATTTAGACGATGTGGGGAAGGACACTTATCACCACACCTTCTTTG
AGATGCTTGGTAACTGGTCTTTTGGTGATTATTTTAAGAACGAAGCTATT
GAATGGACTTGGGAGTTGCTTACCAAGGTTTATAGGTTACCGGCTGATAG
ACTTTATGCTACTTATTTTGGCGGTGATGAGAAAGCTGGGCTTGCACCTG
ATAATGAAGCTAGAGACATTTGGCTCAAGTTTTTACCAGCTTCGCGTG
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