CB417303

Overview
NameCB417303
Unique NameCB417303
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length348
Libraries
Library NameType
Mature peel (flavedo) cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig376 contig Csv1_Contig376:1..348. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_FRATT (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. tularensis GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 57/111 (51.35%), Postives = 72/111 (64.86%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLKFL 335
            K +FLG      +    +RA   QKC+RAGGKHNDLD+VG    HHTFFEMLGN+SFGDYFK EAI + WE LTK  +LP ++L+ T +        A D+EA D+W K +
Sbjct:   47 KDVFLGI-----EKKDFSRAVTVQKCLRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKKEAISFAWEFLTKEIKLPVEKLWVTIY--------ASDDEAFDVWHKHI 144
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_FRATO (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 57/111 (51.35%), Postives = 72/111 (64.86%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLKFL 335
            K +FLG      +    +RA   QKC+RAGGKHNDLD+VG    HHTFFEMLGN+SFGDYFK EAI + WE LTK  +LP ++L+ T +        A D+EA D+W K +
Sbjct:   47 KDVFLGI-----EKKDFSRAVTVQKCLRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKKEAISFAWEFLTKEIKLPVEKLWVTIY--------ASDDEAFDVWHKHI 144
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_FRATM (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. mediasiatica (strain FSC147) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 57/111 (51.35%), Postives = 72/111 (64.86%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLKFL 335
            K +FLG      +    +RA   QKC+RAGGKHNDLD+VG    HHTFFEMLGN+SFGDYFK EAI + WE LTK  +LP ++L+ T +        A D+EA D+W K +
Sbjct:   47 KDVFLGI-----EKKDFSRAVTVQKCLRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKKEAISFAWEFLTKEIKLPVEKLWVTIY--------ASDDEAFDVWHKHI 144
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_FRATH (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. holarctica (strain LVS) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 57/111 (51.35%), Postives = 72/111 (64.86%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLKFL 335
            K +FLG      +    +RA   QKC+RAGGKHNDLD+VG    HHTFFEMLGN+SFGDYFK EAI + WE LTK  +LP ++L+ T +        A D+EA D+W K +
Sbjct:   47 KDVFLGI-----EKKDFSRAVTVQKCLRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKKEAISFAWEFLTKEIKLPVEKLWVTIY--------ASDDEAFDVWHKHI 144
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_FRATF (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 57/111 (51.35%), Postives = 72/111 (64.86%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLKFL 335
            K +FLG      +    +RA   QKC+RAGGKHNDLD+VG    HHTFFEMLGN+SFGDYFK EAI + WE LTK  +LP ++L+ T +        A D+EA D+W K +
Sbjct:   47 KDVFLGI-----EKKDFSRAVTVQKCLRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKKEAISFAWEFLTKEIKLPVEKLWVTIY--------ASDDEAFDVWHKHI 144
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_FRAT1 (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. tularensis (strain FSC 198) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 57/111 (51.35%), Postives = 72/111 (64.86%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLKFL 335
            K +FLG      +    +RA   QKC+RAGGKHNDLD+VG    HHTFFEMLGN+SFGDYFK EAI + WE LTK  +LP ++L+ T +        A D+EA D+W K +
Sbjct:   47 KDVFLGI-----EKKDFSRAVTVQKCLRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKKEAISFAWEFLTKEIKLPVEKLWVTIY--------ASDDEAFDVWHKHI 144
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_DESVV (Alanyl-tRNA synthetase OS=Desulfovibrio vulgaris subsp. vulgaris (strain DP4) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 59/117 (50.43%), Postives = 77/117 (65.81%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLKF--LPASR 347
            K IFLG      +    +RA  +QKC+R GGKHNDL++VG+   HHTFFEMLGN+SFGDYFK +AI++ W+ LT+  +LP +RLYAT F          D+EA  +WLK   +PA R
Sbjct:   47 KKIFLGQ-----EKRAYSRATTSQKCLRVGGKHNDLENVGRTARHHTFFEMLGNFSFGDYFKEDAIKFAWKFLTEELKLPKERLYATVF--------RDDDEAEQLWLKHSDIPAER 150
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_DESVH (Alanyl-tRNA synthetase OS=Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 59/117 (50.43%), Postives = 77/117 (65.81%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLKF--LPASR 347
            K IFLG      +    +RA  +QKC+R GGKHNDL++VG+   HHTFFEMLGN+SFGDYFK +AI++ W+ LT+  +LP +RLYAT F          D+EA  +WLK   +PA R
Sbjct:   47 KKIFLGQ-----EKRAYSRATTSQKCLRVGGKHNDLENVGRTARHHTFFEMLGNFSFGDYFKEDAIKFAWKFLTEELKLPKERLYATVF--------RDDDEAEQLWLKHSDIPAER 150
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_BURVG (Alanyl-tRNA synthetase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 61/117 (52.14%), Postives = 76/117 (64.96%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK--FLPASR 347
            K +FLGT          TRA   Q+ +RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK++AI + WELLT VY+LP D+L+ T +          D+EA DIW K   +PA R
Sbjct:   46 KDVFLGTDQ-----RPYTRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIRFAWELLTTVYQLPKDKLWVTVY--------QEDDEAYDIWAKEVGVPAER 149
[Close]
BLAST of CB417303 vs. ExPASy Swiss-Prot
Match: SYA_BURTA (Alanyl-tRNA synthetase OS=Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) GN=alaS PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 8.118e-26
Identity = 60/117 (51.28%), Postives = 76/117 (64.96%), Query Frame = 3 
Query:    3 KPIFLGTADPNTQLSKLTRAFNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKNEAIEWTWELLTKVYRLPADRLYATYFGGDEKAGLAPDNEARDIWLK--FLPASR 347
            K +FLG      +    +RA   Q+ +RAGGKHNDL++VG    HHTFFEMLGN+SFGDYFK +AI + WELLT VY+LPAD+L+ T +  D+        EA DIW K   +PA R
Sbjct:   46 KDVFLGA-----ETRPYSRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWELLTTVYKLPADKLWVTVYHDDD--------EAYDIWAKEVGVPAER 149
The following BLAST results are available for this feature:
BLAST of CB417303 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
SYA_FRATT8.118e-2651.35Alanyl-tRNA synthetase OS=Francisella tularensis s... [more]
SYA_FRATO8.118e-2651.35Alanyl-tRNA synthetase OS=Francisella tularensis s... [more]
SYA_FRATM8.118e-2651.35Alanyl-tRNA synthetase OS=Francisella tularensis s... [more]
SYA_FRATH8.118e-2651.35Alanyl-tRNA synthetase OS=Francisella tularensis s... [more]
SYA_FRATF8.118e-2651.35Alanyl-tRNA synthetase OS=Francisella tularensis s... [more]
SYA_FRAT18.118e-2651.35Alanyl-tRNA synthetase OS=Francisella tularensis s... [more]
SYA_DESVV8.118e-2650.43Alanyl-tRNA synthetase OS=Desulfovibrio vulgaris s... [more]
SYA_DESVH8.118e-2650.43Alanyl-tRNA synthetase OS=Desulfovibrio vulgaris (... [more]
SYA_BURVG8.118e-2652.14Alanyl-tRNA synthetase OS=Burkholderia vietnamiens... [more]
SYA_BURTA8.118e-2651.28Alanyl-tRNA synthetase OS=Burkholderia thailandens... [more]

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Properties
Property NameValue
Genbank descriptionEST0379 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P024 similar to alanine tRNA ligase (acc# NP_175439), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB417303 ID=CB417303; Name=CB417303; organism=Citrus sinensis; type=EST; length=348bp
ACAAGCCGATTTTTCTTGGAACCGCGGATCCGAATACTCAATTGAGTAAG
CTGACGCGCGCGTTTAACACTCAGAAGTGTATACGCGCCGGTGGTAAGCA
TAATGATTTAGACGATGTGGGGAAGGACACTTATCACCACACCTTCTTTG
AGATGCTTGGTAACTGGTCTTTTGGTGATTATTTTAAGAACGAAGCTATT
GAATGGACTTGGGAGTTGCTTACCAAGGTTTATAGGTTACCGGCTGATAG
ACTTTATGCTACTTATTTTGGCGGTGATGAGAAAGCTGGGCTTGCACCTG
ATAATGAAGCTAGAGACATTTGGCTCAAGTTTTTACCAGCTTCGCGTG
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