CF417199

Overview
NameCF417199
Unique NameCF417199
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length697
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: SPM1_SCHPO (Mitogen-activated protein kinase spm1 OS=Schizosaccharomyces pombe GN=spm1 PE=1 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.775e-13
Identity = 44/159 (27.67%), Postives = 73/159 (45.91%), Query Frame = 3 
Query:   15 YEKLEKVGEGTYGKVYKAKDKATG--QLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQN 485
            ++ ++++G+G YG V  A++ A+   + VA+KK      +  +   ALRE+ LL        +    C+  +D     N                 + +Y+  E ++ DL   I S +      PL     QSF+YQ+  G+ + HS  V+HRDLKP N
Sbjct:   21 FKVVKELGQGAYGIVCAARNVASKDQEAVAIKKITNVFSKSILTKRALREIKLLIHFRNHRNIT---CIYDLDIINPYNF----------------NEVYIYEELMEADLNAIIKSGQ------PLTDAHFQSFIYQILCGLKYIHSANVIHRDLKPGN 154

HSP 2 Score: 39.2762 bits (90), Expect = 2.775e-13
Identity = 21/60 (35.00%), Postives = 34/60 (56.67%), Query Frame = 1 
Query:  505 RGFSRSPILASDAPSPFLSKAIPTRLLLSGIGAPEVLLGSTHYSTAVDMWSVGCIFAEMV 684
            RG S +P    +    F+++ + TR       APE++L  + Y   +D+WSVGCI AE++
Sbjct:  172 RGCSENP----EENPGFMTEYVATRWYR----APEIMLSFSSYHKGIDVWSVGCILAELL 223
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: MK08_MOUSE (Mitogen-activated protein kinase 8 OS=Mus musculus GN=Mapk8 PE=1 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.783e-13
Identity = 43/160 (26.88%), Postives = 71/160 (44.38%), Query Frame = 3 
Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQN 485
            +++Y+ L+ +G G  G V  A D    + VA+KK       +     A RE+ L++ ++    ++ LL V     SL+                    ++Y+V E +D +L + I           L    +   LYQ+  G+ H HS G++HRDLKP N
Sbjct:   23 LKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHK-NIIGLLNVFTPQKSLEEFQ-----------------DVYIVMELMDANLCQVIQME--------LDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSN 156

HSP 2 Score: 37.7354 bits (86), Expect = 2.783e-13
Identity = 18/32 (56.25%), Postives = 22/32 (68.75%), Query Frame = 1 
Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQV 696
            APEV+LG   Y   VD+WSVGCI  EMV  ++
Sbjct:  193 APEVILGMG-YKENVDLWSVGCIMGEMVCHKI 223
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: HOG1_WALSE (Mitogen-activated protein kinase HOG1 (Fragment) OS=Wallemia sebi GN=HOG1 PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.811e-13
Identity = 25/62 (40.32%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:  300 NLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQN 485
            ++Y + E L TDL + + S       RPL    +Q FLYQ+ + +   H  GV+HRDLKP N
Sbjct:   27 DIYFITELLGTDLHRLLSS-------RPLERQFVQYFLYQMLRALKFVHPAGVVHRDLKPSN 81

HSP 2 Score: 44.2838 bits (103), Expect = 2.811e-13
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 1 
Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMV 684
            APE++L    Y +AVD+WSVGCIFAEM+
Sbjct:  116 APEIMLTWQEYDSAVDIWSVGCIFAEMI 143
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: HOG1_WALIC (Mitogen-activated protein kinase HOG1 (Fragment) OS=Wallemia ichthyophaga GN=HOG1 PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.811e-13
Identity = 25/62 (40.32%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:  300 NLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQN 485
            ++Y + E L TDL + + S       RPL    +Q FLYQ+ + +   H  GV+HRDLKP N
Sbjct:   26 DIYFITELLGTDLHRLLSS-------RPLERQFVQYFLYQMLRALKFVHPAGVVHRDLKPSN 80

HSP 2 Score: 44.2838 bits (103), Expect = 2.811e-13
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 1 
Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMV 684
            APE++L    Y +AVD+WSVGCIFAEM+
Sbjct:  115 APEIMLTWQEYDSAVDIWSVGCIFAEMI 142
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: MPK3_ORYSJ (Mitogen-activated protein kinase 3 OS=Oryza sativa subsp. japonica GN=MPK3 PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 3.050e-13
Identity = 63/211 (29.86%), Postives = 90/211 (42.65%), Query Frame = 3 
Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYRGSRGASWFHSLLHCC 644
            KY  ++ +G G YG V  + ++ T + VA+KK     D        LRE+ LL+ L             H +     +   P    +F        ++YLV+E +DTDL + I S      P+ L     Q FL+QL +G+ + HS  +LHRDLKP N               GL R  +   +  T  +VT WYR          LL CC
Sbjct:   31 KYVPIKPIGRGAYGIVCSSINRETNEKVAIKKIHNVFDNRVDALRTLRELKLLRHL------------RHENVIALKDIMMPVHRRSF-------KDVYLVYELMDTDLHQIIKS------PQGLSNDHCQYFLFQLLRGLKYLHSAEILHRDLKPGN-LLVNANCDLKICDFGLARTNSSKGQFMTEYVVTRWYRAP-------ELLLCC 208
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: STK36_PONAB (Serine/threonine-protein kinase 36 OS=Pongo abelii GN=STK36 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.504e-13
Identity = 49/160 (30.63%), Postives = 77/160 (48.12%), Query Frame = 3 
Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQN 485
            MEKY  LE +GEG++G+VYK + K + Q+VALK            P   R    L+ L + + ++R L   ++   L S          F T       + +V +Y + +L + ++   K      LP   +Q+   QL   + + HSH +LHRD+KPQN
Sbjct:    1 MEKYHVLEMIGEGSFGRVYKGRRKYSAQVVALK----------FIPKLGRSEKELRNLQREIEIMRGLRHPNIVHMLDS----------FETD----KEVVVVTDYAEGELLQILEDDGK------LPEDQVQAIAAQLVSALYYLHSHRILHRDMKPQN 130

HSP 2 Score: 26.5646 bits (57), Expect = 3.504e-13
Identity = 12/27 (44.44%), Postives = 16/27 (59.26%), Query Frame = 1 
Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEM 681
            +PE L+    Y    D+WSVGCI  E+
Sbjct:  168 SPE-LVEERPYDHTADLWSVGCILYEL 193
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: MK08_RAT (Mitogen-activated protein kinase 8 OS=Rattus norvegicus GN=Mapk8 PE=1 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 3.607e-13
Identity = 43/160 (26.88%), Postives = 71/160 (44.38%), Query Frame = 3 
Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQN 485
            +++Y+ L+ +G G  G V  A D    + VA+KK       +     A RE+ L++ ++    ++ LL V     SL+                    ++Y+V E +D +L + I           L    +   LYQ+  G+ H HS G++HRDLKP N
Sbjct:   23 LKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHK-NIIGLLNVFTPQKSLEEFQ-----------------DVYIVMELMDANLCQVIQME--------LDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSN 156

HSP 2 Score: 37.3502 bits (85), Expect = 3.607e-13
Identity = 18/28 (64.29%), Postives = 20/28 (71.43%), Query Frame = 1 
Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMV 684
            APEV+LG   Y   VD+WSVGCI  EMV
Sbjct:  193 APEVILGMG-YKENVDLWSVGCIMGEMV 219
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: JNK_DROME (Stress-activated protein kinase JNK OS=Drosophila melanogaster GN=bsk PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 3.618e-13
Identity = 46/160 (28.75%), Postives = 68/160 (42.50%), Query Frame = 3 
Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKS--NLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQN 485
            +Y  L  +G G  G V  A D  T Q VA+KK             A RE  L+++++    ++ LL                   +AF    N +   ++YLV E +D +L + I           L    +   LYQ+  G+ H HS G++HRDLKP N
Sbjct:   23 RYINLRPIGSGAQGIVCAAYDTITQQNVAIKKLSRPFQNVTHAKRAYREFKLMKLVNHK-NIIGLL-------------------NAFTPQRNLEEFQDVYLVMELMDANLCQVIQMD--------LDHDRMSYLLYQMLCGIKHLHSAGIIHRDLKPSN 154

HSP 2 Score: 37.7354 bits (86), Expect = 3.618e-13
Identity = 17/29 (58.62%), Postives = 22/29 (75.86%), Query Frame = 1 
Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVK 687
            APEV+LG   Y+  VD+WSVGCI  EM++
Sbjct:  191 APEVILGMG-YTENVDIWSVGCIMGEMIR 218
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: HOG1_WALMU (Mitogen-activated protein kinase HOG1 (Fragment) OS=Wallemia muriae GN=HOG1 PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 3.651e-13
Identity = 25/62 (40.32%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:  300 NLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQN 485
            ++Y + E L TDL + + S       RPL    +Q FLYQ+ + +   H  GV+HRDLKP N
Sbjct:   27 DIYFITELLGTDLHRLLSS-------RPLERQFVQYFLYQMLRALKFVHPAGVVHRDLKPSN 81

HSP 2 Score: 43.8986 bits (102), Expect = 3.651e-13
Identity = 17/28 (60.71%), Postives = 23/28 (82.14%), Query Frame = 1 
Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMV 684
            APE++L    Y +AVD+WS+GCIFAEM+
Sbjct:  116 APEIMLTWQEYDSAVDIWSIGCIFAEMI 143
[Close]
BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: MPK17_ORYSJ (Mitogen-activated protein kinase 17 OS=Oryza sativa subsp. japonica GN=MPK17 PE=2 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 3.983e-13
Identity = 62/209 (29.67%), Postives = 90/209 (43.06%), Query Frame = 3 
Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAF---TVPLKSYTHEIVTLWYRGSRGASWFHS 629
            +Y+  E +G+G+YG V  A D  TG+ VA+KK     D        LRE+ LL++L             H D     +   P S   F        ++Y++FE +++DL + I ++        L P   Q FLYQL +G+ + H+  V HRDLKP+N               GL R     T     +T  + T WYR       F S
Sbjct:  104 RYKVSEVIGKGSYGVVAAAVDTQTGERVAIKKINDVFDHVSDATRILREIKLLRLL------------RHPDIVEIKHIMLPPSRREF-------RDIYVIFELMESDLHQVIKAN------DDLTPEHHQFFLYQLLRGMKYIHAASVFHRDLKPKN-ILANADCKLKVCDFGLARVSFNDTPSAIFWTDYVATRWYRAPELCGSFFS 286
The following BLAST results are available for this feature:
BLAST of CF417199 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 480
Match NameE-valueIdentityDescription
SPM1_SCHPO2.775e-1327.67Mitogen-activated protein kinase spm1 OS=Schizosac... [more]
MK08_MOUSE2.783e-1326.88Mitogen-activated protein kinase 8 OS=Mus musculus... [more]
HOG1_WALSE2.811e-1340.32Mitogen-activated protein kinase HOG1 (Fragment) O... [more]
HOG1_WALIC2.811e-1340.32Mitogen-activated protein kinase HOG1 (Fragment) O... [more]
MPK3_ORYSJ3.050e-1329.86Mitogen-activated protein kinase 3 OS=Oryza sativa... [more]
STK36_PONAB3.504e-1330.63Serine/threonine-protein kinase 36 OS=Pongo abelii... [more]
MK08_RAT3.607e-1326.88Mitogen-activated protein kinase 8 OS=Rattus norve... [more]
JNK_DROME3.618e-1328.75Stress-activated protein kinase JNK OS=Drosophila ... [more]
HOG1_WALMU3.651e-1340.32Mitogen-activated protein kinase HOG1 (Fragment) O... [more]
MPK17_ORYSJ3.983e-1329.67Mitogen-activated protein kinase 17 OS=Oryza sativ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-129 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-002_E10 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417199 ID=CF417199; Name=CF417199; organism=Citrus sinensis; type=EST; length=697bp
AGAGGATGGAGAAGTACGAGAAGCTAGAGAAGGTGGGAGAAGGAACGTAC
GGGAAAGTGTACAAGGCAAAGGACAAAGCGACCGGGCAGTTGGTTGCTCT
GAAGAAAACAAGACTCGAAATGGATGAAGAAGGCATCCCCCCGACCGCTC
TCCGCGAAGTGTCCCTCCTCCAGATGCTCTCTCAATCCCTTTACGTCGTC
CGCCTCCTCTGCGTCGAACACGTGGACTCCTCTCTCAAATCCAACAACCC
CAGCCCCGACTCCGCTTCCGCCTTCGCCACCTCCAACAACGGCAAGTCTA
ATCTCTATCTCGTGTTTGAATACCTCGACACCGATCTAAAGAAATTCATC
GACTCGCACCGCAAGGGCCCCAATCCGAGGCCGCTGCCCCCCACTCTCAT
TCAGAGCTTCTTGTACCAGCTTTGCAAAGGCGTCGCCCACTGCCACAGCC
ACGGTGTCCTTCATCGCGACCTGAAGCCGCAGAATCTTCTCCTTGATAAG
GACAAGGGGATTCTCAAGATCGCCGATCTTGGCCTCGGACGCGCCTTCAC
CGTTCCTCTCAAAAGCTATACCCACGAGATTGTTACTCTCTGGTATAGGG
GCTCCCGAGGTGCTTCTTGGTTCCACTCATTACTCCACTGCTGTCGACAT
GTGGTCCGTTGGTTGCATTTTCGCTGAGATGGTTAAAAGGCAGGTTT
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