CF417870 | Citrus Genome Database

Overview


Name	CF417870
Unique Name	CF417870
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	705

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: TIP31_ARATH (Aquaporin TIP3-1 OS=Arabidopsis thaliana GN=TIP3-1 PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.396e-14
Identity = 45/123 (36.59%), Postives = 59/123 (47.97%), Query Frame = 3

Query:  339 WLAXCLGAICGCGLVKAFQKSYYXXYGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSAT-DPKRXAXDSHVPVLAPLPIGFAVFMVHLATIPVTGTGIXPARSLGAAVIYSKEKAWDDQWI 704
            W+A  LGAI  C L++                LA G     GL  EII TF LVY V+S   DPKR +    + ++APL IG  V    L   P +G  + PAR+ G A++  +   W D WI
Sbjct:  115 WIAQLLGAILACLLLRLTTNGMRPV----GFRLASGVGAVNGLVLEIILTFGLVYVVYSTLIDPKRGS----LGIIAPLAIGLIVGANILVGGPFSGASMNPARAFGPALVGWR---WHDHWI 226

HSP 2 Score: 37.7354 bits (86), Expect = 1.396e-14
Identity = 20/57 (35.09%), Postives = 31/57 (54.39%), Query Frame = 1

Query:  178 GGVGILGIAWAFGGMIFVLVYCXAGISGGHINPXXTFGLFLARQGVTGASRNVHGWL 348
            GG+ ++ +A AF   +F  V     +SGGH+NP  TFG  +   G   A R ++ W+
Sbjct:   64 GGLILVALAHAFA--LFAAVSAAINVSGGHVNPAVTFGALVG--GRVTAIRAIYYWI 116

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: AQP5_RAT (Aquaporin-5 OS=Rattus norvegicus GN=Aqp5 PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.617e-14
Identity = 40/113 (35.40%), Postives = 60/113 (53.10%), Query Frame = 3

Query:  345 AXCLGAICGCGLVKAFQKSYYXXYGGGA-NELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRXAXDSHVPVLAP-LPIGFAVFMVHLATIPVTGTGIXPARSLGAAVIYSK 677
            A  +GAI G G++  +  +     G  A N L +  + G  +  E+I TF L   +FS+TD +R +     PV +P L IG +V + HL  I  TG  + PARS G AV+ ++
Sbjct:   93 AQLVGAIAGAGIL--YWLAPLNARGNLAVNALNNNTTPGKAMVVELILTFQLALCIFSSTDSRRTS-----PVGSPALSIGLSVTLGHLVGIYFTGCSMNPARSFGPAVVMNR 198

HSP 2 Score: 34.6538 bits (78), Expect = 1.617e-14
Identity = 18/40 (45.00%), Postives = 21/40 (52.50%), Query Frame = 1

Query:  190 ILGIAWAFGGMIFVLVYCXAGISGGHINPXXTFGLFLARQ 309
            IL I+ AFG  I  L      +SGGHINP  T  L +  Q
Sbjct:   42 ILQISIAFGLAIGTLAQALGPVSGGHINPAITLALLIGNQ 81

HSP 3 Score: 28.8758 bits (63), Expect = 1.617e-14
Identity = 11/35 (31.43%), Postives = 24/35 (68.57%), Query Frame = 3

Query:   39 EELTQWSFYRATIAEFIATLLFLYITVLTVIGYKS 143
            +E+   +F++A  AEF+ATL+F++  + + + + S
Sbjct:    3 KEVCSLAFFKAVFAEFLATLIFVFFGLGSALKWPS 37

HSP 4 Score: 21.1718 bits (43), Expect = 1.617e-14
Identity = 8/18 (44.44%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  308 KVSLVRAVMYMAGSXFGS 361
            ++SL+RAV Y+A    G+
Sbjct:   81 QISLLRAVFYVAAQLVGA 98

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: AQP5_PIG (Aquaporin-5 OS=Sus scrofa GN=AQP5 PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.725e-14
Identity = 41/119 (34.45%), Postives = 60/119 (50.42%), Query Frame = 3

Query:  342 LAXCLGAICGCGLVKAFQKSYYXXYGGGA------NELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRXAXDSHVPVLAP-LPIGFAVFMVHLATIPVTGTGIXPARSLGAAVIYSK 677
            +A  +GAI G G++       Y    G A      N L +  + G  +  E+I TF L   +FS+TD +R +     PV +P L IG +V + HL  I  TG  + PARS G AV+ ++
Sbjct:   92 VAQLVGAIAGAGIL-------YGLAPGNARGNLAVNSLNNNTTPGQAVVVEMILTFQLALCIFSSTDSRRTS-----PVGSPALSIGLSVTLGHLVGIYFTGCSMNPARSFGPAVVMNR 198

HSP 2 Score: 35.8094 bits (81), Expect = 1.725e-14
Identity = 19/40 (47.50%), Postives = 21/40 (52.50%), Query Frame = 1

Query:  190 ILGIAWAFGGMIFVLVYCXAGISGGHINPXXTFGLFLARQ 309
            IL IA AFG  I  L      +SGGHINP  T  L +  Q
Sbjct:   42 ILQIALAFGLAIGTLAQALGPVSGGHINPAITLALLVGNQ 81

HSP 3 Score: 28.4906 bits (62), Expect = 1.725e-14
Identity = 11/35 (31.43%), Postives = 23/35 (65.71%), Query Frame = 3

Query:   39 EELTQWSFYRATIAEFIATLLFLYITVLTVIGYKS 143
            +E+   +F +A  AEF+ATL+F++  + + + + S
Sbjct:    3 KEVCSLAFLKAVFAEFLATLIFVFFGLASALKWPS 37

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: AQY1_YEAST (Aquaporin-1 OS=Saccharomyces cerevisiae GN=AQY1 PE=1 SV=2)

HSP 1 Score: 60.4622 bits (145), Expect = 2.344e-14
Identity = 36/80 (45.00%), Postives = 44/80 (55.00%), Query Frame = 3

Query:  426 ANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRXAXDSHVPVLAPLPIGFAVFMVHLATIPVTGTGIXPARSLGAAV 665
            AN L  G S   GL  E+ GT +L  TV      KR         +A LPIG ++F+ H+A    TGTG+ PARSLGAAV
Sbjct:  165 ANSLGLGCSRTRGLFLEMFGTAILCLTVLMTAVEKRETN-----FMAALPIGISLFIAHVALTAYTGTGVNPARSLGAAV 239

HSP 2 Score: 37.3502 bits (85), Expect = 2.344e-14
Identity = 22/63 (34.92%), Postives = 33/63 (52.38%), Query Frame = 1

Query:  190 ILGIAWAFGGMIFVLVYCXAGISGGHINPXXTFGLFLARQGVTGASRNVHGWLXVWEPYVAAG 378
            ++ IA  FG  +   ++C AG+SGG +NP  +  L LAR      +R V  W+      +AAG
Sbjct:   91 LIMIAIGFGFSVMFSIWCFAGVSGGALNPAVSLSLCLAR--AVSPTRCVVMWVSQIVAGMAAG 151

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: TIP22_ARATH (Probable aquaporin TIP2-2 OS=Arabidopsis thaliana GN=TIP2-2 PE=1 SV=2)

HSP 1 Score: 61.6178 bits (148), Expect = 3.068e-14
Identity = 42/111 (37.84%), Postives = 58/111 (52.25%), Query Frame = 3

Query:  339 WLAXCLGAICGCGLVKAFQKSYYXXYGGGANELADGYSTGTGLGAEIIGTFVLVYTVFS-ATDPKRXAXDSHVPVLAPLPIGFAVFMVHLATIPVTGTGIXPARSLGAAVI 668
            W+A CLG+I  C L+            G    +A G     G+  EI+ TF LVYTV++ A DPK+ +  +    +AP+ IGF V    LA  P +G  + PARS G AV+
Sbjct:  105 WIAQCLGSIVACLLLVFVTNGESVPTHG----VAAGLGAIEGVVMEIVVTFALVYTVYATAADPKKGSLGT----IAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVV 207

HSP 2 Score: 35.8094 bits (81), Expect = 3.068e-14
Identity = 21/51 (41.18%), Postives = 28/51 (54.90%), Query Frame = 1

Query:  142 ARLTPTTAVMRCGGVGILGIAWAFGGMIFVLVYCXAGISGGHINPXXTFGL 294
            A+LT   A+      G++ +A A    +FV V   A ISGGH+NP  T GL
Sbjct:   43 AKLTSDAAL---DPAGLVAVAVAHAFALFVGVSIAANISGGHLNPAVTLGL 90

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: TIP23_ARATH (Aquaporin TIP2-3 OS=Arabidopsis thaliana GN=TIP2-3 PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 5.179e-14
Identity = 41/111 (36.94%), Postives = 58/111 (52.25%), Query Frame = 3

Query:  339 WLAXCLGAICGCGLVKAFQKSYYXXYGGGANELADGYSTGTGLGAEIIGTFVLVYTVFS-ATDPKRXAXDSHVPVLAPLPIGFAVFMVHLATIPVTGTGIXPARSLGAAVI 668
            W+A CLG+I  C L+            G    ++ G     G+  EI+ TF LVYTV++ A DPK+ +  +    +AP+ IGF V    LA  P +G  + PARS G AV+
Sbjct:  105 WIAQCLGSIVACLLLVFVTNGKSVPTHG----VSAGLGAVEGVVMEIVVTFALVYTVYATAADPKKGSLGT----IAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVV 207

HSP 2 Score: 35.8094 bits (81), Expect = 5.179e-14
Identity = 18/36 (50.00%), Postives = 22/36 (61.11%), Query Frame = 1

Query:  187 GILGIAWAFGGMIFVLVYCXAGISGGHINPXXTFGL 294
            G++ IA A    +FV V   A ISGGH+NP  T GL
Sbjct:   55 GLVAIAIAHAFALFVGVSIAANISGGHLNPAVTLGL 90

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: TIP22_ORYSJ (Probable aquaporin TIP2-2 OS=Oryza sativa subsp. japonica GN=TIP2-2 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 5.179e-14
Identity = 42/110 (38.18%), Postives = 55/110 (50.00%), Query Frame = 3

Query:  339 WLAXCLGAICGCGLVKAFQKSYYXXYGGGANELADGYSTGTGLGAEIIGTFVLVYTVFS-ATDPKRXAXDSHVPVLAPLPIGFAVFMVHLATIPVTGTGIXPARSLGAAV 665
            W+A  LGAI G  LV+         +G        G     G+  EII TF LVYTV++ A DPK+ +  +    +AP+ IGF V    L   P +G  + PARS G AV
Sbjct:  106 WIAQLLGAIVGAVLVQFCTGVATPTHG------LSGVGAFEGVVMEIIVTFGLVYTVYATAADPKKGSLGT----IAPIAIGFIVGANILVAGPFSGGSMNPARSFGPAV 205

HSP 2 Score: 41.2022 bits (95), Expect = 5.179e-14
Identity = 20/41 (48.78%), Postives = 25/41 (60.98%), Query Frame = 1

Query:  187 GILGIAWAFGGMIFVLVYCXAGISGGHINPXXTFGLFLARQ 309
            G++ +A   G  +FV V   A ISGGH+NP  TFGL L  Q
Sbjct:   56 GLVAVAVCHGFGLFVAVAIGANISGGHVNPAVTFGLALGGQ 96

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: AQP5_SHEEP (Aquaporin-5 OS=Ovis aries GN=AQP5 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 6.248e-14
Identity = 42/113 (37.17%), Postives = 58/113 (51.33%), Query Frame = 3

Query:  342 LAXCLGAICGCGLVKAFQKSYYXXYGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRXAXDSHVPVLAP-LPIGFAVFMVHLATIPVTGTGIXPARSLGAAVIYSK 677
            +A  +GAI G  ++      Y        N L +  + G  + AE+I TF L   VFS+TD +R +     PV +P L IG +V + HL  I  TG  + PARS G AVI S+
Sbjct:   92 VAQLVGAIAGAAILYGLAP-YNARSNLAVNALNNNTTAGQAVVAEMILTFQLALCVFSSTDSRRTS-----PVGSPALSIGLSVTLGHLVGIYFTGCSMNPARSFGPAVIMSR 198

HSP 2 Score: 32.3426 bits (72), Expect = 6.248e-14
Identity = 15/40 (37.50%), Postives = 21/40 (52.50%), Query Frame = 1

Query:  190 ILGIAWAFGGMIFVLVYCXAGISGGHINPXXTFGLFLARQ 309
            +L I+ AFG  I  +      +SGGH+NP  T  L +  Q
Sbjct:   42 VLQISLAFGLAIGTMAQALGPVSGGHMNPAITLALLVGNQ 81

HSP 3 Score: 26.9498 bits (58), Expect = 6.248e-14
Identity = 11/35 (31.43%), Postives = 23/35 (65.71%), Query Frame = 3

Query:   39 EELTQWSFYRATIAEFIATLLFLYITVLTVIGYKS 143
            +E+   +F +A  AEF+ATL+F++  + + + + S
Sbjct:    3 KEVCSAAFLKAVFAEFLATLIFVFFGLGSALKWPS 37

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: TIP1_MEDTR (Probable aquaporin TIP-type OS=Medicago truncatula GN=AQP1 PE=1 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 8.741e-14
Identity = 41/122 (33.61%), Postives = 61/122 (50.00%), Query Frame = 3

Query:  342 LAXCLGAICGCGLVKAFQKSYYXXYGGGANELADGYSTGTGLGAEIIGTFVLVYTVFS-ATDPKRXAXDSHVPVLAPLPIGFAVFMVHLATIPVTGTGIXPARSLGAAVIYSKEKAWDDQWI 704
            +A  LG+I    L+     S    +G     L++G   G  L  EI+ TF LVYTV++ A DPK+     ++ ++AP+ IGF V    L     TG  + PA S G AV+     +W + W+
Sbjct:  108 IAQLLGSIVASALLVFVTASSVPAFG-----LSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKK----GNIGIIAPIAIGFIVGANILVGGAFTGASMNPAVSFGPAVV---SWSWSNHWV 217

HSP 2 Score: 37.7354 bits (86), Expect = 8.741e-14
Identity = 17/38 (44.74%), Postives = 23/38 (60.53%), Query Frame = 1

Query:  187 GILGIAWAFGGMIFVLVYCXAGISGGHINPXXTFGLFL 300
            G++  + A    +FV V   A ISGGH+NP  TFG F+
Sbjct:   57 GLISASIAHAFALFVAVSVGANISGGHVNPAVTFGAFV 94

[Close]

BLAST of CF417870 vs. ExPASy Swiss-Prot
Match: AQP2_HUMAN (Aquaporin-2 OS=Homo sapiens GN=AQP2 PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.044e-13
Identity = 39/120 (32.50%), Postives = 57/120 (47.50%), Query Frame = 3

Query:  345 AXCLGAICGCGLVKAFQKSYYXXYGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRXAXDSHVPVLAPLPIGFAVFMVHLATIPVTGTGIXPARSLGAAVIYSKEKAWDDQWI 704
            A  LGA+ G  L+     +         N L++  + G  +  E+  T  LV  +F++TD +R       P    L IGF+V + HL  I  TG  + PARSL  AV+  K   +DD W+
Sbjct:   92 AQLLGAVAGAALLHEITPADIRG-DLAVNALSNSTTAGQAVTVELFLTLQLVLCIFASTDERRGEN----PGTPALSIGFSVALGHLLGIHYTGCSMNPARSLAPAVVTGK---FDDHWV 203

HSP 2 Score: 30.8018 bits (68), Expect = 1.044e-13
Identity = 17/32 (53.12%), Postives = 18/32 (56.25%), Query Frame = 1

Query:  190 ILGIAWAFGGMIFVLVYCXAGISGGHINPXXT 285
            +L IA AFG  I  LV     ISG HINP  T
Sbjct:   41 VLQIAMAFGLGIGTLVQALGHISGAHINPAVT 72

HSP 3 Score: 28.8758 bits (63), Expect = 1.044e-13
Identity = 13/23 (56.52%), Postives = 17/23 (73.91%), Query Frame = 3

Query:   42 ELTQWSFYRATIAEFIATLLFLY 110
            EL   +F RA  AEF+ATLLF++
Sbjct:    3 ELRSIAFSRAVFAEFLATLLFVF 25

The following BLAST results are available for this feature:

BLAST of CF417870 vs. ExPASy Swiss-Prot

BLAST of CF417870 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 98


Match Name	E-value	Identity	Description
TIP31_ARATH	1.396e-14	36.59	Aquaporin TIP3-1 OS=Arabidopsis thaliana GN=TIP3-1...	[more]
AQP5_RAT	1.617e-14	35.40	Aquaporin-5 OS=Rattus norvegicus GN=Aqp5 PE=2 SV=1	[more]
AQP5_PIG	1.725e-14	34.45	Aquaporin-5 OS=Sus scrofa GN=AQP5 PE=2 SV=1	[more]
AQY1_YEAST	2.344e-14	45.00	Aquaporin-1 OS=Saccharomyces cerevisiae GN=AQY1 PE...	[more]
TIP22_ARATH	3.068e-14	37.84	Probable aquaporin TIP2-2 OS=Arabidopsis thaliana ...	[more]
TIP23_ARATH	5.179e-14	36.94	Aquaporin TIP2-3 OS=Arabidopsis thaliana GN=TIP2-3...	[more]
TIP22_ORYSJ	5.179e-14	38.18	Probable aquaporin TIP2-2 OS=Oryza sativa subsp. j...	[more]
AQP5_SHEEP	6.248e-14	37.17	Aquaporin-5 OS=Ovis aries GN=AQP5 PE=2 SV=1	[more]
TIP1_MEDTR	8.741e-14	33.61	Probable aquaporin TIP-type OS=Medicago truncatula...	[more]
AQP2_HUMAN	1.044e-13	32.50	Aquaporin-2 OS=Homo sapiens GN=AQP2 PE=1 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_115000-800 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-011_B01 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417870 ID=CF417870; Name=CF417870; organism=Citrus sinensis; type=EST; length=705bp
ATTACCACGACCCTCCTCCAGCTCCATTGATCGATGCCGAGGAGCTCACC
CAATGGTCATTTTACAGGGCTACTATAGCTGAGTTCATCGCCACGCTCTT
GTTTTTGTACATTACTGTGCTGACAGTGATTGGTTACAAGAGCCAGACTG
ACGCCAACCACGGCGGTGATGAGATGTGGTGGTGTTGGCATTCTGGGCAT
CGCTTGGGCCTTTGGTGGCATGATCTTTGTTCTTGTTTACTGCNCTGCTG
GTATCTCTGGNGGACACATTAACCCGNCGGNGACATTTGGGCTGTTCTTG
GCGCGGCAAGGTGTCACTGGTGCGAGCCGTAATGTACATGGCTGGCTCAN
TGTTTGGGAGCCATATGTGGCTGCGGGCTCGTTAAGGCTTTCCAGAAGTC
TTACTACNCNANATACGGCGGNGGAGCCAATGAGCTCGCTGATGGGTACA
GCACTGGCACTGGATTGGGTGCTGAGATCATCGGTACCTTTGTTCTTGTC
TACACTGTTTTCTCCGCCACTGATCCCAAGAGANATGCTANGGACTCCCA
CGTTCCCGTCTTGGCGCCATTGCCNATTGGATTTGCTGTGTTCATGGTTC
ACTTGGCTACGATCCCTGTCACTGGAACTGGCATCNACCCCGCTAGAAGT
TTGGGAGCTGCAGTGATTTACAGCAAGGAGAAGGCTTGGGATGATCAATG
GATCT