CF504235 | Citrus Genome Database

Overview


Name	CF504235
Unique Name	CF504235
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	540

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: PPK14_SCHPO (Serine/threonine-protein kinase ppk14 OS=Schizosaccharomyces pombe GN=ppk14 PE=1 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 3.240e-18
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EY+APE+IKG GH SAVDWWT GI  YE+
Sbjct:  387 EYIAPEVIKGCGHTSAVDWWTLGILFYEM 415

HSP 2 Score: 48.1358 bits (113), Expect = 3.240e-18
Identity = 25/69 (36.23%), Postives = 39/69 (56.52%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPE---SPVVSFSARDLIKGLLVKEPQNRLACRRGA 367
            LG  +   L+  TPFKG     T  N++ + + FPE   +P +S   ++LI+ LLVK+  +RL  + GA
Sbjct:  408 LGILFYEMLYATTPFKGKNRNMTFSNILHKDVIFPEYADAPSISSLCKNLIRKLLVKDENDRLGSQAGA 476

HSP 3 Score: 29.6462 bits (65), Expect = 3.240e-18
Identity = 11/26 (42.31%), Postives = 17/26 (65.38%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISLCKPARDPE 449
            ++K HPFF+ V WAL+   +P   P+
Sbjct:  478 DVKLHPFFKNVQWALLRHTEPPIIPK 503

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: OXI1_ARATH (Serine/threonine-protein kinase OXI1 OS=Arabidopsis thaliana GN=OXI1 PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 2.090e-15
Identity = 18/29 (62.07%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EY+APE+I G+GH  AVDWW+ G+ LYE+
Sbjct:  241 EYVAPEVISGDGHDFAVDWWSLGVVLYEM 269

HSP 2 Score: 44.669 bits (104), Expect = 2.090e-15
Identity = 23/60 (38.33%), Postives = 33/60 (55.00%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRL 349
            LG      L+G TPF+GS  + T Y ++ +P   P     + S RDLI+ LL K+P  R+
Sbjct:  262 LGVVLYEMLYGATPFRGSNRKETFYRILSKP---PNLTGETTSLRDLIRRLLEKDPSRRI 318

HSP 3 Score: 26.1794 bits (56), Expect = 2.090e-15
Identity = 10/18 (55.56%), Postives = 13/18 (72.22%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISL 425
            EIK H FF+GV+W  + L
Sbjct:  322 EIKGHDFFRGVDWEKVIL 339

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: KPK1_ARATH (Serine/threonine-protein kinase AtPK1/AtPK6 OS=Arabidopsis thaliana GN=ATPK1 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 4.575e-14
Identity = 19/30 (63.33%), Postives = 25/30 (83.33%), Query Frame = 1

Query:  103 TEYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            TEY+APEI++G+GH  A DWW+ GI LYE+
Sbjct:  295 TEYMAPEIVRGKGHDKAADWWSVGILLYEM 324

HSP 2 Score: 35.039 bits (79), Expect = 4.575e-14
Identity = 21/60 (35.00%), Postives = 32/60 (53.33%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRL 349
            +G      L GK PF GS+ +     +V   ++ P+   +S  A  ++KGLL KEP+ RL
Sbjct:  317 VGILLYEMLTGKPPFLGSKGKIQ-QKIVKDKIKLPQ--FLSNEAHAILKGLLQKEPERRL 373

HSP 3 Score: 28.8758 bits (63), Expect = 4.575e-14
Identity = 13/30 (43.33%), Postives = 18/30 (60.00%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISL--CKPARDPETS 455
            EIKQH +F+G+NW  +      P+  PE S
Sbjct:  382 EIKQHKWFKGINWKKLEAREVMPSFKPEVS 411

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: PKGA_DICDI (Probable serine/threonine-protein kinase pkgA OS=Dictyostelium discoideum GN=pkgA PE=3 SV=1)

HSP 1 Score: 43.8986 bits (102), Expect = 7.306e-14
Identity = 16/28 (57.14%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYE 189
            +YLAPEI+ G GHG++ DW++ G+ LYE
Sbjct: 1144 DYLAPEILLGIGHGASADWFSLGVILYE 1171

HSP 2 Score: 40.817 bits (94), Expect = 7.306e-14
Identity = 23/60 (38.33%), Postives = 34/60 (56.67%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRL 349
            LG     +L G +PF GS  + T  N++ + + +PE   +S  ARDLI  LL  +P+ RL
Sbjct: 1165 LGVILYEFLCGVSPFNGSSVQETFQNILQRNISWPED--MSPEARDLIDKLLALDPRQRL 1222

HSP 3 Score: 30.8018 bits (68), Expect = 7.306e-14
Identity = 12/26 (46.15%), Postives = 16/26 (61.54%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISLCKPARDPE 449
            EIK HPFF+ +NW  I   +P   P+
Sbjct: 1229 EIKSHPFFKSINWKTILTQEPYFKPK 1254

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: KPK19_ARATH (Serine/threonine-protein kinase AtPK19 OS=Arabidopsis thaliana GN=ATPK19 PE=2 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 7.724e-13
Identity = 19/30 (63.33%), Postives = 25/30 (83.33%), Query Frame = 1

Query:  103 TEYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            TEY+APEI++G+GH  A DWW+ GI LYE+
Sbjct:  301 TEYMAPEIVRGKGHDKAADWWSVGILLYEM 330

HSP 2 Score: 35.8094 bits (81), Expect = 7.724e-13
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRL 349
            +G      L GK PF GS+ +     +V   ++ P+   +S  A  L+KGLL KEP+ RL
Sbjct:  323 VGILLYEMLTGKPPFLGSKGKIQ-QKIVKDKIKLPQ--FLSNEAHALLKGLLQKEPERRL 379

HSP 3 Score: 23.8682 bits (50), Expect = 7.724e-13
Identity = 7/13 (53.85%), Postives = 11/13 (84.62%), Query Frame = 3

Query:  372 EIKQHPFFQGVNW 410
            EIK+H +F+ +NW
Sbjct:  388 EIKKHKWFKAINW 400

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: GAD8_SCHPO (Serine/threonine-protein kinase gad8 OS=Schizosaccharomyces pombe GN=gad8 PE=1 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 5.954e-12
Identity = 17/29 (58.62%), Postives = 22/29 (75.86%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EYLAPE++ G G+   VDWWT G+ LYE+
Sbjct:  393 EYLAPELLLGHGYTKVVDWWTLGVLLYEM 421

HSP 2 Score: 36.1946 bits (82), Expect = 5.954e-12
Identity = 19/60 (31.67%), Postives = 29/60 (48.33%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRL 349
            LG      + G  PF           ++  PLRFP++  +   A+DL+ GLL + P+ RL
Sbjct:  414 LGVLLYEMITGLPPFYDENINEMYRKILQDPLRFPDN--IDEKAKDLLSGLLTRAPEKRL 471

HSP 3 Score: 25.7942 bits (55), Expect = 5.954e-12
Identity = 8/13 (61.54%), Postives = 10/13 (76.92%), Query Frame = 3

Query:  372 EIKQHPFFQGVNW 410
            EIK HPFF  ++W
Sbjct:  478 EIKNHPFFDDIDW 490

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: PK3_DICDI (Protein kinase 3 OS=Dictyostelium discoideum GN=pkgC PE=3 SV=2)

HSP 1 Score: 50.8322 bits (120), Expect = 1.256e-11
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EYLAPEII G GHG A DWW+ GI L+E+
Sbjct:  670 EYLAPEIITGAGHGKAADWWSVGILLFEM 698

HSP 2 Score: 33.4982 bits (75), Expect = 1.256e-11
Identity = 20/65 (30.77%), Postives = 32/65 (49.23%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRRG 364
            +G      L G++PF  S       +++   LR P    +S  A+DL++ LLV +P  RL   +G
Sbjct:  691 VGILLFEMLTGRSPFLASNRNDMYKSMIQGNLRMPM--FLSSDAQDLLEKLLVPDPNKRLGSTQG 753

HSP 3 Score: 23.483 bits (49), Expect = 1.256e-11
Identity = 7/16 (43.75%), Postives = 11/16 (68.75%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALI 419
            EI  HPFF+ + W ++
Sbjct:  756 EISSHPFFELIPWRML 771

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: Y0670_DICDI (Probable serine/threonine-protein kinase DDB_G0288795 OS=Dictyostelium discoideum GN=DDB_G0288795 PE=2 SV=1)

HSP 1 Score: 43.8986 bits (102), Expect = 2.130e-11
Identity = 16/29 (55.17%), Postives = 20/29 (68.97%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EY+APEII G G+  +VDWW  G   YE+
Sbjct:  336 EYVAPEIINGTGYSKSVDWWALGTLFYEM 364

HSP 2 Score: 33.8834 bits (76), Expect = 2.130e-11
Identity = 18/60 (30.00%), Postives = 30/60 (50.00%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRL 349
            LG+ +   + G  PF   +       ++  PL+FP  P +S +A  L+  LL ++P  RL
Sbjct:  357 LGTLFYEMIAGLPPFYSEDPHEMTKLILSSPLKFP--PQISKNAASLVSLLLNRDPSKRL 414

HSP 3 Score: 29.261 bits (64), Expect = 2.130e-11
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 3

Query:  357 DEEQPEIKQHPFFQGVNWA 413
            + +  EIK HPFF+ VNW+
Sbjct:  418 ESDVEEIKAHPFFKNVNWS 436

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: KS6A4_HUMAN (Ribosomal protein S6 kinase alpha-4 OS=Homo sapiens GN=RPS6KA4 PE=1 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 3.521e-11
Identity = 20/30 (66.67%), Postives = 25/30 (83.33%), Query Frame = 1

Query:  106 EYLAPEIIKGE-GHGSAVDWWTFGIFLYEL 192
            EY+APEII+ + GHG AVDWW+ GI L+EL
Sbjct:  202 EYMAPEIIRSKTGHGKAVDWWSLGILLFEL 231

HSP 2 Score: 29.261 bits (64), Expect = 3.521e-11
Identity = 21/62 (33.87%), Postives = 30/62 (48.39%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRF--PESPVVSFSARDLIKGLLVKEPQNRL 349
            LG      L G +PF     R T   V  + L+   P  P +   A+DL++ LL K+P+ RL
Sbjct:  224 LGILLFELLTGASPFTLEGERNTQAEVSRRILKCSPPFPPRIGPVAQDLLQRLLCKDPKKRL 285

HSP 3 Score: 29.261 bits (64), Expect = 3.521e-11
Identity = 9/20 (45.00%), Postives = 15/20 (75.00%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISLCK 431
            E++ HPFFQG++W  ++  K
Sbjct:  294 EVRNHPFFQGLDWVALAARK 313

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: AUR2_ARATH (Serine/threonine-protein kinase Aurora-2 OS=Arabidopsis thaliana GN=AUR2 PE=2 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 4.490e-11
Identity = 27/64 (42.19%), Postives = 37/64 (57.81%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRR 361
            LG     +L+G  PF+  E+  T   +V   L+FP  P+VS SA+DLI  +LVKE   RLA  +
Sbjct:  200 LGILCYEFLYGVPPFEAREHSETYKRIVQVDLKFPPKPIVSSSAKDLISQMLVKESTQRLALHK 263

HSP 2 Score: 33.113 bits (74), Expect = 4.490e-11
Identity = 12/28 (42.86%), Postives = 19/28 (67.86%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYE 189
            +YL PE+++   H ++VD W+ GI  YE
Sbjct:  179 DYLPPEMVESVEHDASVDIWSLGILCYE 206

The following BLAST results are available for this feature:

BLAST of CF504235 vs. ExPASy Swiss-Prot

BLAST of CF504235 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 25


Match Name	E-value	Identity	Description
PPK14_SCHPO	3.240e-18	72.41	Serine/threonine-protein kinase ppk14 OS=Schizosac...	[more]
OXI1_ARATH	2.090e-15	62.07	Serine/threonine-protein kinase OXI1 OS=Arabidopsi...	[more]
KPK1_ARATH	4.575e-14	63.33	Serine/threonine-protein kinase AtPK1/AtPK6 OS=Ara...	[more]
PKGA_DICDI	7.306e-14	57.14	Probable serine/threonine-protein kinase pkgA OS=D...	[more]
KPK19_ARATH	7.724e-13	63.33	Serine/threonine-protein kinase AtPK19 OS=Arabidop...	[more]
GAD8_SCHPO	5.954e-12	58.62	Serine/threonine-protein kinase gad8 OS=Schizosacc...	[more]
PK3_DICDI	1.256e-11	68.97	Protein kinase 3 OS=Dictyostelium discoideum GN=pk...	[more]
Y0670_DICDI	2.130e-11	55.17	Probable serine/threonine-protein kinase DDB_G0288...	[more]
KS6A4_HUMAN	3.521e-11	66.67	Ribosomal protein S6 kinase alpha-4 OS=Homo sapien...	[more]
AUR2_ARATH	4.490e-11	42.19	Serine/threonine-protein kinase Aurora-2 OS=Arabid...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-724 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-16_A01 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504235 ID=CF504235; Name=CF504235; organism=Citrus sinensis; type=EST; length=540bp
AAGCACAGAAGCCTAAGAATGAAATGGGGAACCAAGTGAGCCCATTGCCA
GAACTTATTGCTGAGCCAACCAATGCCCGCTCCATGTCTTTTGTGGGAAC
ACACTGAGTACCTGGCACCGGAAATCATCAAAGGTGAAGGGCATGGCAGT
GCTGTTGATTGGTGGACTTTTGGGATCTTTCTATATGAGCTACCTGTTCG
GTAAAACTCCTTTTAAAGGATCTGAGAATAGGGCCACCTTGTATAATGTT
GTGGGGCAACCGTTGCGATTTCCAGAGTCACCTGTTGTTAGTTTTTCAGC
AAGGGATTTGATAAAGGGCTTACTAGTGAAAGAGCCCCAGAATAGATTAG
CCTGCAGACGAGGAGCAACCCGAGATCAAGCAACATCCCTTCTTTCAAGG
TGTAAATTGGGCATTGATTTCGTTGTGCAAGCCCGCCAGAGATCCCGAAA
CCAGTTGAGATTGTCCATAGTCCAGCCCCAGCATTATCAACTAAGGCAAA
AGCTGTTGCTGCTGGTAATGCTGATCATAATAATTATTTG