CF504354

Overview
NameCF504354
Unique NameCF504354
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length401
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN2_ORYSJ (GTP-binding nuclear protein Ran-2 OS=Oryza sativa subsp. japonica GN=RAN2 PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 5.922e-58
Identity = 87/89 (97.75%), Postives = 88/89 (98.88%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDF TNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVT+RLTYKNVPTWHRDLCR
Sbjct:   25 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFTTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRDLCR 113

HSP 2 Score: 50.0618 bits (118), Expect = 5.922e-58
Identity = 23/24 (95.83%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:   62 MALPNQQTVDYPSFKLVIVGDGGT 133
            MALPNQ TVDYPSFKLVIVGDGGT
Sbjct:    1 MALPNQGTVDYPSFKLVIVGDGGT 24
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN2_ORYSI (GTP-binding nuclear protein Ran-2 OS=Oryza sativa subsp. indica GN=RAN2 PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 5.922e-58
Identity = 87/89 (97.75%), Postives = 88/89 (98.88%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDF TNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVT+RLTYKNVPTWHRDLCR
Sbjct:   25 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFTTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRDLCR 113

HSP 2 Score: 50.0618 bits (118), Expect = 5.922e-58
Identity = 23/24 (95.83%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:   62 MALPNQQTVDYPSFKLVIVGDGGT 133
            MALPNQ TVDYPSFKLVIVGDGGT
Sbjct:    1 MALPNQGTVDYPSFKLVIVGDGGT 24
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN1B_LOTJA (GTP-binding nuclear protein Ran1B (Fragment) OS=Lotus japonicus GN=RAN1B PE=2 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 3.398e-52
Identity = 89/89 (100.00%), Postives = 89/89 (100.00%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR
Sbjct:   13 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 101

HSP 2 Score: 26.5646 bits (57), Expect = 3.398e-52
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 2 
Query:   98 SFKLVIVGDGGT 133
            +FKLVIVGDGGT
Sbjct:    1 NFKLVIVGDGGT 12
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN1A_LOTJA (GTP-binding nuclear protein Ran1A (Fragment) OS=Lotus japonicus GN=RAN1A PE=2 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 3.398e-52
Identity = 89/89 (100.00%), Postives = 89/89 (100.00%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR
Sbjct:   13 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 101

HSP 2 Score: 26.5646 bits (57), Expect = 3.398e-52
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 2 
Query:   98 SFKLVIVGDGGT 133
            +FKLVIVGDGGT
Sbjct:    1 NFKLVIVGDGGT 12
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN3_ORYSJ (GTP-binding nuclear protein Ran-3 OS=Oryza sativa subsp. japonica GN=RAN3 PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 5.672e-49
Identity = 77/89 (86.52%), Postives = 84/89 (94.38%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRH+TGEFEK+YEPTIGVEV PLDF T+ GK+RF CWDTAGQEKFGGLRDGYYIHG CAIIMFDVT+RLTYKNVPTWH+D+CR
Sbjct:   29 GKTTFVKRHITGEFEKRYEPTIGVEVRPLDFHTSRGKVRFCCWDTAGQEKFGGLRDGYYIHGHCAIIMFDVTSRLTYKNVPTWHKDICR 117

HSP 2 Score: 38.891 bits (89), Expect = 5.672e-49
Identity = 16/27 (59.26%), Postives = 21/27 (77.78%), Query Frame = 2 
Query:   53 NQLMALPNQQTVDYPSFKLVIVGDGGT 133
            ++  ALP+   V YPSFKL++VGDGGT
Sbjct:    2 SRAQALPDPAAVGYPSFKLILVGDGGT 28
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN3_ORYSI (GTP-binding nuclear protein Ran-3 OS=Oryza sativa subsp. indica GN=RAN3 PE=2 SV=2)

HSP 1 Score: 176.407 bits (446), Expect = 5.672e-49
Identity = 77/89 (86.52%), Postives = 84/89 (94.38%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRH+TGEFEK+YEPTIGVEV PLDF T+ GK+RF CWDTAGQEKFGGLRDGYYIHG CAIIMFDVT+RLTYKNVPTWH+D+CR
Sbjct:   29 GKTTFVKRHITGEFEKRYEPTIGVEVRPLDFHTSRGKVRFCCWDTAGQEKFGGLRDGYYIHGHCAIIMFDVTSRLTYKNVPTWHKDICR 117

HSP 2 Score: 38.891 bits (89), Expect = 5.672e-49
Identity = 16/27 (59.26%), Postives = 21/27 (77.78%), Query Frame = 2 
Query:   53 NQLMALPNQQTVDYPSFKLVIVGDGGT 133
            ++  ALP+   V YPSFKL++VGDGGT
Sbjct:    2 SRAQALPDPAAVGYPSFKLILVGDGGT 28
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN4_ARATH (GTP-binding nuclear protein Ran-4 OS=Arabidopsis thaliana GN=RAN4 PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 5.019e-44
Identity = 68/89 (76.40%), Postives = 77/89 (86.52%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTF+KRHLTGEFE   EPT+GV+++PLDFFTN GKIRF CWDTAGQEK+ GL+D YYIHGQCAIIMFDVTAR TY N+  W+RDL R
Sbjct:   25 GKTTFLKRHLTGEFEHNTEPTLGVDIYPLDFFTNRGKIRFECWDTAGQEKYSGLKDAYYIHGQCAIIMFDVTARHTYMNIDRWYRDLRR 113

HSP 2 Score: 45.4394 bits (106), Expect = 5.019e-44
Identity = 20/24 (83.33%), Postives = 22/24 (91.67%), Query Frame = 2 
Query:   62 MALPNQQTVDYPSFKLVIVGDGGT 133
            MALPNQQ VD P+FKL+IVGDGGT
Sbjct:    1 MALPNQQNVDLPTFKLLIVGDGGT 24
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: GSP2_YEAST (GTP-binding nuclear protein GSP2/CNR2 OS=Saccharomyces cerevisiae GN=GSP2 PE=1 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.448e-43
Identity = 74/89 (83.15%), Postives = 80/89 (89.89%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  TIGVEVHPL F+TN G+I+F  WDTAGQEKFGGLRDGYYI+ QCAIIMFDVT+R+TYKNVP WHRDL R
Sbjct:   25 GKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGEIKFDVWDTAGQEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVR 113

HSP 2 Score: 34.6538 bits (78), Expect = 1.448e-43
Identity = 14/24 (58.33%), Postives = 18/24 (75.00%), Query Frame = 2 
Query:   62 MALPNQQTVDYPSFKLVIVGDGGT 133
            M+ P Q   + P+FKLV+VGDGGT
Sbjct:    1 MSAPAQNNAEVPTFKLVLVGDGGT 24
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN_CAEEL (GTP-binding nuclear protein ran-1 OS=Caenorhabditis elegans GN=ran-1 PE=1 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 4.524e-42
Identity = 76/89 (85.39%), Postives = 79/89 (88.76%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  T+GVEVHPL F TN G+IRF  WDTAGQEKFGGLRDGYYI GQCAIIMFDVTAR+TYKNVP WHRDL R
Sbjct:   21 GKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGQIRFNVWDTAGQEKFGGLRDGYYIQGQCAIIMFDVTARVTYKNVPNWHRDLAR 109

HSP 2 Score: 28.4906 bits (62), Expect = 4.524e-42
Identity = 11/13 (84.62%), Postives = 13/13 (100.00%), Query Frame = 2 
Query:   95 PSFKLVIVGDGGT 133
            P+FKLV+VGDGGT
Sbjct:    8 PTFKLVLVGDGGT 20
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: GSP1_YEAST (GTP-binding nuclear protein GSP1/CNR1 OS=Saccharomyces cerevisiae GN=GSP1 PE=1 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 7.680e-42
Identity = 74/89 (83.15%), Postives = 80/89 (89.89%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  TIGVEVHPL F+TN G+I+F  WDTAGQEKFGGLRDGYYI+ QCAIIMFDVT+R+TYKNVP WHRDL R
Sbjct:   24 GKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGEIKFDVWDTAGQEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVR 112

HSP 2 Score: 28.8758 bits (63), Expect = 7.680e-42
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 2 
Query:   89 DYPSFKLVIVGDGGT 133
            + P+FKLV+VGDGGT
Sbjct:    9 EVPTFKLVLVGDGGT 23
The following BLAST results are available for this feature:
BLAST of CF504354 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 55
Match NameE-valueIdentityDescription
RAN2_ORYSJ5.922e-5897.75GTP-binding nuclear protein Ran-2 OS=Oryza sativa ... [more]
RAN2_ORYSI5.922e-5897.75GTP-binding nuclear protein Ran-2 OS=Oryza sativa ... [more]
RAN1B_LOTJA3.398e-52100.00GTP-binding nuclear protein Ran1B (Fragment) OS=Lo... [more]
RAN1A_LOTJA3.398e-52100.00GTP-binding nuclear protein Ran1A (Fragment) OS=Lo... [more]
RAN3_ORYSJ5.672e-4986.52GTP-binding nuclear protein Ran-3 OS=Oryza sativa ... [more]
RAN3_ORYSI5.672e-4986.52GTP-binding nuclear protein Ran-3 OS=Oryza sativa ... [more]
RAN4_ARATH5.019e-4476.40GTP-binding nuclear protein Ran-4 OS=Arabidopsis t... [more]
GSP2_YEAST1.448e-4383.15GTP-binding nuclear protein GSP2/CNR2 OS=Saccharom... [more]
RAN_CAEEL4.524e-4285.39GTP-binding nuclear protein ran-1 OS=Caenorhabditi... [more]
GSP1_YEAST7.680e-4283.15GTP-binding nuclear protein GSP1/CNR1 OS=Saccharom... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-843 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-17_D02 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504354 ID=CF504354; Name=CF504354; organism=Citrus sinensis; type=EST; length=401bp
ATTCCCCGACACTGAGCGCTCTCTCGCTTAAATTCAAAATCCAAACGCAA
CAAATCAACTCATGGCTCTGCCGAATCAGCAAACTGTTGATTATCCAAGC
TTCAAACTTGTAATTGTCGGCGATGGTGGCACCTGGGAAGACTACCTTTG
TGAAGAGGCATCTTACTGGTGAATTCGAGAAGAAATATGAACCGACCATT
GGTGTGGAAGTTCATCCATTGGACTTCTTTACCAACTGTGGAAAAATACG
ATTCTATTGCTGGGACACTGCCGGACAAGAGAAGTTTGGTGGTCTTAGAG
ATGGATACTACATCCATGGGCAATGTGCTATCATCATGTTTGATGTTACT
GCCCGCTTGACGTATAAGAATGTTCCGACGTGGCACCGTGATCTCTGCAG
G
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