CF504354

Overview
NameCF504354
Unique NameCF504354
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length401
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN_DROME (GTP-binding nuclear protein Ran OS=Drosophila melanogaster GN=ran PE=1 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 2.215e-41
Identity = 73/89 (82.02%), Postives = 78/89 (87.64%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRH+TGEFEKKY  T+GVEVHPL F TN G IRF  WDTAGQEKFGGLRDGYYI GQCA+IMFDVT+R+TYKNVP WHRDL R
Sbjct:   22 GKTTFVKRHMTGEFEKKYVATLGVEVHPLIFHTNRGAIRFNVWDTAGQEKFGGLRDGYYIQGQCAVIMFDVTSRVTYKNVPNWHRDLVR 110

HSP 2 Score: 29.6462 bits (65), Expect = 2.215e-41
Identity = 12/19 (63.16%), Postives = 15/19 (78.95%), Query Frame = 2 
Query:   77 QQTVDYPSFKLVIVGDGGT 133
            Q+  D P+FK V+VGDGGT
Sbjct:    3 QEGQDIPTFKCVLVGDGGT 21
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN_ONCVO (GTP-binding nuclear protein Ran OS=Onchocerca volvulus GN=ran-1 PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 3.760e-41
Identity = 74/89 (83.15%), Postives = 78/89 (87.64%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTG+ EKKY  T+GVEVHPL F TN G+IRF  WDTAGQEKFGGLRDGYYI GQCAIIMFDVTAR+TYKNVP WHRDL R
Sbjct:   21 GKTTFVKRHLTGDPEKKYVATLGVEVHPLIFHTNRGQIRFNVWDTAGQEKFGGLRDGYYIQGQCAIIMFDVTARVTYKNVPNWHRDLAR 109

HSP 2 Score: 30.4166 bits (67), Expect = 3.760e-41
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 2 
Query:   89 DYPSFKLVIVGDGGT 133
            D P+FKLV+VGDGGT
Sbjct:    6 DIPTFKLVLVGDGGT 20
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: GSP1_ASHGO (GTP-binding nuclear protein GSP1/Ran OS=Ashbya gossypii GN=GSP1 PE=3 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 3.761e-41
Identity = 73/89 (82.02%), Postives = 79/89 (88.76%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  TIGVEVHPL F+TN G+I+F  WDTAGQEKFGGLRDGYYI+ QC IIMFDVT+R+TYKNVP WHRDL R
Sbjct:   19 GKTTFVKRHLTGEFEKKYIATIGVEVHPLAFYTNFGEIKFDVWDTAGQEKFGGLRDGYYINAQCGIIMFDVTSRITYKNVPNWHRDLVR 107

HSP 2 Score: 28.8758 bits (63), Expect = 3.761e-41
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 2 
Query:   89 DYPSFKLVIVGDGGT 133
            + P+FKLV+VGDGGT
Sbjct:    4 EVPTFKLVLVGDGGT 18
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: GSP1_NEUCR (GTP-binding nuclear protein GSP1/Ran OS=Neurospora crassa GN=gsp-1 PE=2 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 4.899e-41
Identity = 73/89 (82.02%), Postives = 79/89 (88.76%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  T+GVEVHPL F TN G+I+F  WDTAGQEKFGGLRDGYYI+GQC IIMFDVT+R+TYKNVP WHRDL R
Sbjct:   19 GKTTFVKRHLTGEFEKKYMATLGVEVHPLGFSTNFGQIQFDVWDTAGQEKFGGLRDGYYINGQCGIIMFDVTSRITYKNVPNWHRDLTR 107

HSP 2 Score: 28.4906 bits (62), Expect = 4.899e-41
Identity = 11/13 (84.62%), Postives = 13/13 (100.00%), Query Frame = 2 
Query:   95 PSFKLVIVGDGGT 133
            P+FKLV+VGDGGT
Sbjct:    6 PTFKLVLVGDGGT 18
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN_BRUMA (GTP-binding nuclear protein Ran OS=Brugia malayi PE=2 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 6.384e-41
Identity = 73/89 (82.02%), Postives = 77/89 (86.52%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTG+FEKKY  T+GVEVHPL F TN G+IRF  WDTAGQEKFGGLRDGYYI GQCA  MFDVTAR+TYKNVP WHRDL R
Sbjct:   21 GKTTFVKRHLTGDFEKKYVATLGVEVHPLIFHTNRGQIRFNVWDTAGQEKFGGLRDGYYIQGQCAFNMFDVTARVTYKNVPNWHRDLVR 109

HSP 2 Score: 30.4166 bits (67), Expect = 6.384e-41
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 2 
Query:   89 DYPSFKLVIVGDGGT 133
            D P+FKLV+VGDGGT
Sbjct:    6 DIPTFKLVLVGDGGT 20
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: GSP1_CANGA (GTP-binding nuclear protein GSP1/Ran OS=Candida glabrata GN=GSP1 PE=3 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 8.320e-41
Identity = 73/89 (82.02%), Postives = 78/89 (87.64%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  TIGVEVHPL F TN G+I+F  WDTAGQEKFGGLRDGYYI+ QC IIMFDVT+R+TYKNVP WHRDL R
Sbjct:   19 GKTTFVKRHLTGEFEKKYIATIGVEVHPLAFHTNFGEIKFDVWDTAGQEKFGGLRDGYYINAQCGIIMFDVTSRITYKNVPNWHRDLVR 107

HSP 2 Score: 29.261 bits (64), Expect = 8.320e-41
Identity = 11/17 (64.71%), Postives = 15/17 (88.24%), Query Frame = 2 
Query:   83 TVDYPSFKLVIVGDGGT 133
            + + P+FKLV+VGDGGT
Sbjct:    2 SAEVPTFKLVLVGDGGT 18
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: GSP1_CANAL (GTP-binding nuclear protein GSP1/Ran OS=Candida albicans GN=GSP1 PE=3 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 3.124e-40
Identity = 71/89 (79.78%), Postives = 79/89 (88.76%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  T+GVEV+PL F TN G+++F  WDTAGQEKFGGLRDGYYI+GQC IIMFDVT+R+TYKNVP WHRDL R
Sbjct:   19 GKTTFVKRHLTGEFEKKYIATLGVEVNPLGFHTNFGELKFDVWDTAGQEKFGGLRDGYYINGQCGIIMFDVTSRITYKNVPNWHRDLVR 107

HSP 2 Score: 28.8758 bits (63), Expect = 3.124e-40
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 2 
Query:   89 DYPSFKLVIVGDGGT 133
            + P+FKLV+VGDGGT
Sbjct:    4 EVPTFKLVLVGDGGT 18
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN_XENTR (GTP-binding nuclear protein Ran OS=Xenopus tropicalis GN=ran PE=2 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 1.991e-39
Identity = 73/89 (82.02%), Postives = 77/89 (86.52%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  T+GVEVHPL F TN G I+F  WDTAGQEKFGGLRDGYYI  QCAIIMFDVT+R+TYKNVP WHRDL R
Sbjct:   22 GKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVR 110

HSP 2 Score: 25.409 bits (54), Expect = 1.991e-39
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 2 
Query:  101 FKLVIVGDGGT 133
            FKLV+VGDGGT
Sbjct:   11 FKLVLVGDGGT 21
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN_XENLA (GTP-binding nuclear protein Ran OS=Xenopus laevis GN=ran PE=1 SV=2)

HSP 1 Score: 157.918 bits (398), Expect = 1.991e-39
Identity = 73/89 (82.02%), Postives = 77/89 (86.52%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  T+GVEVHPL F TN G I+F  WDTAGQEKFGGLRDGYYI  QCAIIMFDVT+R+TYKNVP WHRDL R
Sbjct:   22 GKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVR 110

HSP 2 Score: 25.409 bits (54), Expect = 1.991e-39
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 2 
Query:  101 FKLVIVGDGGT 133
            FKLV+VGDGGT
Sbjct:   11 FKLVLVGDGGT 21
[Close]
BLAST of CF504354 vs. ExPASy Swiss-Prot
Match: RAN_RAT (GTP-binding nuclear protein Ran OS=Rattus norvegicus GN=Ran PE=1 SV=3)

HSP 1 Score: 157.918 bits (398), Expect = 1.991e-39
Identity = 73/89 (82.02%), Postives = 77/89 (86.52%), Query Frame = 3 
Query:  135 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR 401
            GKTTFVKRHLTGEFEKKY  T+GVEVHPL F TN G I+F  WDTAGQEKFGGLRDGYYI  QCAIIMFDVT+R+TYKNVP WHRDL R
Sbjct:   22 GKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVR 110

HSP 2 Score: 25.409 bits (54), Expect = 1.991e-39
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 2 
Query:  101 FKLVIVGDGGT 133
            FKLV+VGDGGT
Sbjct:   11 FKLVLVGDGGT 21
The following BLAST results are available for this feature:
BLAST of CF504354 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 55
Match NameE-valueIdentityDescription
RAN_DROME2.215e-4182.02GTP-binding nuclear protein Ran OS=Drosophila mela... [more]
RAN_ONCVO3.760e-4183.15GTP-binding nuclear protein Ran OS=Onchocerca volv... [more]
GSP1_ASHGO3.761e-4182.02GTP-binding nuclear protein GSP1/Ran OS=Ashbya gos... [more]
GSP1_NEUCR4.899e-4182.02GTP-binding nuclear protein GSP1/Ran OS=Neurospora... [more]
RAN_BRUMA6.384e-4182.02GTP-binding nuclear protein Ran OS=Brugia malayi P... [more]
GSP1_CANGA8.320e-4182.02GTP-binding nuclear protein GSP1/Ran OS=Candida gl... [more]
GSP1_CANAL3.124e-4079.78GTP-binding nuclear protein GSP1/Ran OS=Candida al... [more]
RAN_XENTR1.991e-3982.02GTP-binding nuclear protein Ran OS=Xenopus tropica... [more]
RAN_XENLA1.991e-3982.02GTP-binding nuclear protein Ran OS=Xenopus laevis ... [more]
RAN_RAT1.991e-3982.02GTP-binding nuclear protein Ran OS=Rattus norvegic... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-843 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-17_D02 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504354 ID=CF504354; Name=CF504354; organism=Citrus sinensis; type=EST; length=401bp
ATTCCCCGACACTGAGCGCTCTCTCGCTTAAATTCAAAATCCAAACGCAA
CAAATCAACTCATGGCTCTGCCGAATCAGCAAACTGTTGATTATCCAAGC
TTCAAACTTGTAATTGTCGGCGATGGTGGCACCTGGGAAGACTACCTTTG
TGAAGAGGCATCTTACTGGTGAATTCGAGAAGAAATATGAACCGACCATT
GGTGTGGAAGTTCATCCATTGGACTTCTTTACCAACTGTGGAAAAATACG
ATTCTATTGCTGGGACACTGCCGGACAAGAGAAGTTTGGTGGTCTTAGAG
ATGGATACTACATCCATGGGCAATGTGCTATCATCATGTTTGATGTTACT
GCCCGCTTGACGTATAAGAATGTTCCGACGTGGCACCGTGATCTCTGCAG
G
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