CF504368

Overview
NameCF504368
Unique NameCF504368
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length336
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_MOUSE (Cytoplasmic aconitate hydratase OS=Mus musculus GN=Aco1 PE=1 SV=2)

HSP 1 Score: 100.523 bits (249), Expect = 5.907e-24
Identity = 43/80 (53.75%), Postives = 56/80 (70.00%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+P MFK  Y+ I   N +WN L+ P+ KLY+W+P STYI  PP+F+ +T+D      + DAY LLN GDS+TTDHISPA
Sbjct:  604 VIPGMFKEVYQKIETVNKSWNALAAPSEKLYAWNPKSTYIKSPPFFESLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPA 683

HSP 2 Score: 29.6462 bits (65), Expect = 5.907e-24
Identity = 14/33 (42.42%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      +  P   YL  RG+  R+FNSYGS
Sbjct:  679 HISPAGNIARNSPAARYLTNRGLTPREFNSYGS 711
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_CHICK (Cytoplasmic aconitate hydratase OS=Gallus gallus GN=ACO1 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.867e-23
Identity = 43/80 (53.75%), Postives = 54/80 (67.50%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+P MFK  Y+ I   N  WN L  P+ KLY+W+P STYI  PP+F  +T+       ++DAY LLNFGDS+TTDHISPA
Sbjct:  604 VIPGMFKEVYQKIETVNEAWNALDAPSDKLYTWNPKSTYIKSPPFFDGLTLALQTPKTIEDAYVLLNFGDSVTTDHISPA 683

HSP 2 Score: 29.6462 bits (65), Expect = 2.867e-23
Identity = 14/33 (42.42%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      +  P   YL  RG+  R+FNSYGS
Sbjct:  679 HISPAGNIARNSPAARYLTSRGLTPREFNSYGS 711
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_RABIT (Cytoplasmic aconitate hydratase OS=Oryctolagus cuniculus GN=ACO1 PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.854e-23
Identity = 42/80 (52.50%), Postives = 55/80 (68.75%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+P MF   Y+ I   N +WN L+ P+ KLY W+P STYI  PP+F+++T+D      + DAY LLN GDS+TTDHISPA
Sbjct:  604 VIPGMFTEVYQKIETVNASWNALAAPSDKLYLWNPKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPA 683

HSP 2 Score: 29.6462 bits (65), Expect = 4.854e-23
Identity = 14/33 (42.42%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      +  P   YL  RG+  R+FNSYGS
Sbjct:  679 HISPAGNIARNSPAARYLTNRGLTPREFNSYGS 711
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_HUMAN (Cytoplasmic aconitate hydratase OS=Homo sapiens GN=ACO1 PE=1 SV=3)

HSP 1 Score: 95.1301 bits (235), Expect = 2.354e-22
Identity = 41/80 (51.25%), Postives = 55/80 (68.75%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+P MFK  Y+ I   N +WN L+ P+ KL+ W+  STYI  PP+F+++T+D      + DAY LLN GDS+TTDHISPA
Sbjct:  604 VIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPA 683

HSP 2 Score: 29.6462 bits (65), Expect = 2.354e-22
Identity = 14/33 (42.42%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      +  P   YL  RG+  R+FNSYGS
Sbjct:  679 HISPAGNIARNSPAARYLTNRGLTPREFNSYGS 711
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON_BACSU (Aconitate hydratase OS=Bacillus subtilis GN=citB PE=1 SV=4)

HSP 1 Score: 82.8037 bits (203), Expect = 2.676e-20
Identity = 34/81 (41.98%), Postives = 51/81 (62.96%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            +V P++F+  YE +   N  WN++      LY WD +STYI  PP+F++M+++P     +K    +  FGDS+TTDHISPA
Sbjct:  616 TVTPELFRKEYETVFDDNKRWNEIETTDEALYKWDNDSTYIQNPPFFEEMSVEPGKVEPLKGLRVVGKFGDSVTTDHISPA 696

HSP 2 Score: 35.039 bits (79), Expect = 2.676e-20
Identity = 17/33 (51.52%), Postives = 18/33 (54.55%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      K  P   YL E+GV  RDFNSYGS
Sbjct:  692 HISPAGAIGKDTPAGKYLQEKGVSPRDFNSYGS 724
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_CAEEL (Probable cytoplasmic aconitate hydratase OS=Caenorhabditis elegans GN=aco-1 PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 3.482e-20
Identity = 40/80 (50.00%), Postives = 49/80 (61.25%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V P  F+  Y  I  G+  W QL  PA KLY WD  STYI + P+F  MT + P    + +A+ LLN GDS+TTDHISPA
Sbjct:  602 VKPQFFREVYANIELGSTEWQQLECPAVKLYPWDDASTYIKKVPFFDGMTSELPSQSDIVNAHVLLNLGDSVTTDHISPA 681

HSP 2 Score: 31.5722 bits (70), Expect = 3.482e-20
Identity = 15/33 (45.45%), Postives = 19/33 (57.58%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S     +KT P   +L  RGV  RDFN+YG+
Sbjct:  677 HISPAGSISKTSPAARFLAGRGVTPRDFNTYGA 709
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON_LEGPH (Aconitate hydratase OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) GN=acn PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.059e-18
Identity = 34/76 (44.74%), Postives = 49/76 (64.47%), Query Frame = 3 
Query:   18 MFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            MF+  Y  + KG+  W  +   + + Y W+P+STYI  PP+F+++++ P     +K AY L  FGDSITTDHISPA
Sbjct:  605 MFRKEYAEVFKGDAHWQAIQTSSGQTYEWNPDSTYIQHPPFFENLSLKPEPLKPIKQAYVLALFGDSITTDHISPA 680

HSP 2 Score: 30.0314 bits (66), Expect = 1.059e-18
Identity = 12/21 (57.14%), Postives = 15/21 (71.43%), Query Frame = 2 
Query:  269 PLQSYLLERGVERRDFNSYGS 331
            P   YL  +GV+ +DFNSYGS
Sbjct:  688 PAGLYLKSKGVDEKDFNSYGS 708
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON_CORJK (Aconitate hydratase OS=Corynebacterium jeikeium (strain K411) GN=acn PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.789e-18
Identity = 36/81 (44.44%), Postives = 49/81 (60.49%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            S+  D++   Y  + +G+  W  L VP+ K + WDP STYI + PYF  M+ +P   + VK A  L   GDS+TTDHISPA
Sbjct:  638 SITKDLYAEDYANVFEGDERWRSLDVPSGKTFDWDPKSTYIRKAPYFDGMSKEPEAVNDVKGARVLALLGDSVTTDHISPA 718

HSP 2 Score: 28.1054 bits (61), Expect = 1.789e-18
Identity = 13/33 (39.39%), Postives = 16/33 (48.48%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S         P   YL   GVER+D+NS G+
Sbjct:  714 HISPASTIKPGTPAAQYLDANGVERKDYNSLGA 746
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACON_COREF (Aconitate hydratase OS=Corynebacterium efficiens GN=acn PE=2 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 5.113e-18
Identity = 33/77 (42.86%), Postives = 50/77 (64.94%), Query Frame = 3 
Query:   15 DMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            +++++ Y  + KG+  W +L +P+ K + WD NSTYI + PYF  MT +P     +++A  L   GDS+TTDHISPA
Sbjct:  645 ELYEADYADVFKGDKQWQELDIPSGKTFEWDENSTYIRKAPYFDGMTAEPQPVTDIENARVLAKLGDSVTTDHISPA 721

HSP 2 Score: 28.1054 bits (61), Expect = 5.113e-18
Identity = 14/33 (42.42%), Postives = 16/33 (48.48%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S         P   YL   GVER+D+NS GS
Sbjct:  717 HISPASSIKPGTPAAQYLDAHGVERQDYNSLGS 749
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: IREB2_RAT (Iron-responsive element-binding protein 2 OS=Rattus norvegicus GN=Ireb2 PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 1.460e-17
Identity = 37/80 (46.25%), Postives = 50/80 (62.50%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+  MFK+  E +  GN  WN L  P S L+ WD  STYI  P +F  +T +P  +  +++A+ LL  GDS+TTDHISPA
Sbjct:  679 VILSMFKALKEKVEMGNKRWNSLDAPDSVLFPWDVKSTYIRCPSFFDKLTKEPAASQPIENAHVLLYLGDSVTTDHISPA 758

HSP 2 Score: 26.5646 bits (57), Expect = 1.460e-17
Identity = 12/33 (36.36%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      ++     YL  RG+  R+FNSYG+
Sbjct:  754 HISPAGSIARSSAAAKYLTNRGLTPREFNSYGA 786
The following BLAST results are available for this feature:
BLAST of CF504368 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48
Match NameE-valueIdentityDescription
ACOC_MOUSE5.907e-2453.75Cytoplasmic aconitate hydratase OS=Mus musculus GN... [more]
ACOC_CHICK2.867e-2353.75Cytoplasmic aconitate hydratase OS=Gallus gallus G... [more]
ACOC_RABIT4.854e-2352.50Cytoplasmic aconitate hydratase OS=Oryctolagus cun... [more]
ACOC_HUMAN2.354e-2251.25Cytoplasmic aconitate hydratase OS=Homo sapiens GN... [more]
ACON_BACSU2.676e-2041.98Aconitate hydratase OS=Bacillus subtilis GN=citB P... [more]
ACOC_CAEEL3.482e-2050.00Probable cytoplasmic aconitate hydratase OS=Caenor... [more]
ACON_LEGPH1.059e-1844.74Aconitate hydratase OS=Legionella pneumophila subs... [more]
ACON_CORJK1.789e-1844.44Aconitate hydratase OS=Corynebacterium jeikeium (s... [more]
ACON_COREF5.113e-1842.86Aconitate hydratase OS=Corynebacterium efficiens G... [more]
IREB2_RAT1.460e-1746.25Iron-responsive element-binding protein 2 OS=Rattu... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-857 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-17_E07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504368 ID=CF504368; Name=CF504368; organism=Citrus sinensis; type=EST; length=336bp
CCAGTGTGTTGCCAGACATGTTCAAAAGTACCTATGAGGCTATCACAAAG
GGAAATCCCACGTGGAATCAGTTATCTGTACCAGCTAGTAAACTCTATTC
TTGGGACCCTAACTCAACCTACATTCATGAACCTCCTTACTTCAAGGACA
TGACTATGGATCCTCCTGGAGCCCATGGAGTTAAGGATGCCTACTGCTTG
CTGAACTTTGGTGACAGTATCACTACAGATCACATTTCTCCGGCGAGGAA
GTATCCACAAAGACAGTCCCACTGCAAAGTTACCTGCTAGAGCGTGGTGT
GGAACGCAGGGACTTCAATTCTTATGGTAGTCCGTC
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