CF504496 | Citrus Genome Database

Overview


Name	CF504496
Unique Name	CF504496
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	587

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8_PIG (26S protease regulatory subunit 8 OS=Sus scrofa GN=PSMC5 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.826e-15
Identity = 25/47 (53.19%), Postives = 42/47 (89.36%), Query Frame = 2

Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y+ +GG+ KQ+ +I+E++ELP++HP+LF+++G+  PKG+LLYGPPG+
Sbjct:  148 YEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGT 194

HSP 2 Score: 31.9574 bits (71), Expect = 2.826e-15
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  195 GKTLLARAVAHHTDCTFIRVSGSELVQK 222

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8_MOUSE (26S protease regulatory subunit 8 OS=Mus musculus GN=Psmc5 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.826e-15
Identity = 25/47 (53.19%), Postives = 42/47 (89.36%), Query Frame = 2

Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y+ +GG+ KQ+ +I+E++ELP++HP+LF+++G+  PKG+LLYGPPG+
Sbjct:  148 YEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGT 194

HSP 2 Score: 31.9574 bits (71), Expect = 2.826e-15
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  195 GKTLLARAVAHHTDCTFIRVSGSELVQK 222

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8_HUMAN (26S protease regulatory subunit 8 OS=Homo sapiens GN=PSMC5 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.826e-15
Identity = 25/47 (53.19%), Postives = 42/47 (89.36%), Query Frame = 2

Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y+ +GG+ KQ+ +I+E++ELP++HP+LF+++G+  PKG+LLYGPPG+
Sbjct:  148 YEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGT 194

HSP 2 Score: 31.9574 bits (71), Expect = 2.826e-15
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  195 GKTLLARAVAHHTDCTFIRVSGSELVQK 222

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8_BOVIN (26S protease regulatory subunit 8 OS=Bos taurus GN=PSMC5 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.826e-15
Identity = 25/47 (53.19%), Postives = 42/47 (89.36%), Query Frame = 2

Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y+ +GG+ KQ+ +I+E++ELP++HP+LF+++G+  PKG+LLYGPPG+
Sbjct:  148 YEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGT 194

HSP 2 Score: 31.9574 bits (71), Expect = 2.826e-15
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  195 GKTLLARAVAHHTDCTFIRVSGSELVQK 222

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS7_SCHPO (26S protease regulatory subunit 7 homolog OS=Schizosaccharomyces pombe GN=rpt1 PE=1 SV=2)

HSP 1 Score: 67.781 bits (164), Expect = 3.666e-15
Identity = 28/50 (56.00%), Postives = 40/50 (80.00%), Query Frame = 2

Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            +V Y DVGG ++Q+ ++RE+VELPL  P+ F  +G+ PPKGI+LYGPPG+
Sbjct:  175 DVTYGDVGGCKEQIERLREVVELPLLSPERFVKLGIDPPKGIMLYGPPGT 224

HSP 2 Score: 32.7278 bits (73), Expect = 3.666e-15
Identity = 16/28 (57.14%), Postives = 19/28 (67.86%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL ARAVAN T A F  + G E++ K
Sbjct:  225 GKTLCARAVANRTDATFIRVIGSELVQK 252

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8_NAEFO (26S protease regulatory subunit 8 homolog OS=Naegleria fowleri PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.671e-15
Identity = 24/53 (45.28%), Postives = 45/53 (84.91%), Query Frame = 2

Query:  344 RLDEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            ++ +  Y+ VGG+ +Q+ +I+E++ELP++HP+LF+S+G+  PKG+++YGPPG+
Sbjct:  149 KVPDATYEMVGGLDEQIKEIKEVIELPIKHPELFESLGIAQPKGVIMYGPPGT 201

HSP 2 Score: 31.9574 bits (71), Expect = 3.671e-15
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  202 GKTLLARAVAHHTDCTFIRVSGSELVQK 229

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS6B_ARATH (26S protease regulatory subunit 6B homolog OS=Arabidopsis thaliana GN=RPT3 PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 3.672e-15
Identity = 29/50 (58.00%), Postives = 39/50 (78.00%), Query Frame = 2

Query:  353 EVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            +V Y+D+GG   Q  +IRE VELPL H +L+K IG+ PP+G+LLYGPPG+
Sbjct:  151 DVSYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGT 200

HSP 2 Score: 30.8018 bits (68), Expect = 3.672e-15
Identity = 14/28 (50.00%), Postives = 19/28 (67.86%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKT++A+AVAN T A F  + G E + K
Sbjct:  201 GKTMLAKAVANHTTAAFIRVVGSEFVQK 228

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PSMR_SULSO (Proteasome-activating nucleotidase OS=Sulfolobus solfataricus GN=pan PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.675e-15
Identity = 28/49 (57.14%), Postives = 44/49 (89.80%), Query Frame = 2

Query:  356 VGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            V Y ++GG+ +Q+ ++RE+VELPL++P++F+ IGV+PPKG+LLYGPPG+
Sbjct:  131 VTYSEIGGLEEQIRELREVVELPLKNPEIFREIGVEPPKGVLLYGPPGT 179

HSP 2 Score: 26.9498 bits (58), Expect = 3.675e-15
Identity = 12/28 (42.86%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKT++A+AVA E+ A F  +   E   K
Sbjct:  180 GKTMLAKAVATESNAVFIHVVASEFAQK 207

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8_DROME (26S protease regulatory subunit 8 OS=Drosophila melanogaster GN=Pros45 PE=1 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 4.773e-15
Identity = 26/47 (55.32%), Postives = 41/47 (87.23%), Query Frame = 2

Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y+ VGG+ KQ+ +I+E++ELP++HP+LF ++G+  PKG+LLYGPPG+
Sbjct:  147 YEMVGGLDKQIKEIKEVIELPVKHPELFDALGIAQPKGVLLYGPPGT 193

HSP 2 Score: 31.5722 bits (70), Expect = 4.773e-15
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  194 GKTLLARAVAHHTECTFIRVSGSELVQK 221

[Close]

BLAST of CF504496 vs. ExPASy Swiss-Prot
Match: PRS8_MANSE (26S protease regulatory subunit 8 OS=Manduca sexta PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 4.773e-15
Identity = 26/47 (55.32%), Postives = 41/47 (87.23%), Query Frame = 2

Query:  362 YDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGS 502
            Y+ VGG+ KQ+ +I+E++ELP++HP+LF ++G+  PKG+LLYGPPG+
Sbjct:  144 YEMVGGLDKQIKEIKEVIELPVKHPELFDALGIAQPKGVLLYGPPGT 190

HSP 2 Score: 31.5722 bits (70), Expect = 4.773e-15
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  504 GKTLIARAVANETGAFFFCINGPEIMSK 587
            GKTL+ARAVA+ T   F  ++G E++ K
Sbjct:  191 GKTLLARAVAHHTECTFIRVSGSELVQK 218

The following BLAST results are available for this feature:

BLAST of CF504496 vs. ExPASy Swiss-Prot

BLAST of CF504496 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 155


Match Name	E-value	Identity	Description
PRS8_PIG	2.826e-15	53.19	26S protease regulatory subunit 8 OS=Sus scrofa GN...	[more]
PRS8_MOUSE	2.826e-15	53.19	26S protease regulatory subunit 8 OS=Mus musculus ...	[more]
PRS8_HUMAN	2.826e-15	53.19	26S protease regulatory subunit 8 OS=Homo sapiens ...	[more]
PRS8_BOVIN	2.826e-15	53.19	26S protease regulatory subunit 8 OS=Bos taurus GN...	[more]
PRS7_SCHPO	3.666e-15	56.00	26S protease regulatory subunit 7 homolog OS=Schiz...	[more]
PRS8_NAEFO	3.671e-15	45.28	26S protease regulatory subunit 8 homolog OS=Naegl...	[more]
PRS6B_ARATH	3.672e-15	58.00	26S protease regulatory subunit 6B homolog OS=Arab...	[more]
PSMR_SULSO	3.675e-15	57.14	Proteasome-activating nucleotidase OS=Sulfolobus s...	[more]
PRS8_DROME	4.773e-15	55.32	26S protease regulatory subunit 8 OS=Drosophila me...	[more]
PRS8_MANSE	4.773e-15	55.32	26S protease regulatory subunit 8 OS=Manduca sexta...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-985 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-19_A03 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504496 ID=CF504496; Name=CF504496; organism=Citrus sinensis; type=EST; length=587bp
CCCCCGGGCTGCAGGGTCGTGAGGTCGAACTTGAGGGTTAGACTCGGAGA
TGTTGTTTCTGTGCACCAGTGCGCTGATGTCAAATACGGAAAGCGTGTGC
ACATATTACCTGTGGATGACACAATTGAAGGGGTTACCTGGAAATCTCTT
TGATGCATACTTGAAACCTTATTTCACGGAAGCTTATCGACCAGTGAGGA
AGGGTGATCTATTCCTTGTTAGAGGTGGAATGAGAAGTGTAGAGTTCAAG
GTTATTGAGACTGACCCTCCTGAATACTGTGTGGTTGCCCCTGATACTGA
GATCTTCTGTGAGGGAGAGCCAGTGAGAAGGGAAGATGAGAATCGATTAG
ATGAGGTTGGCTATGATGATGTTGGTGGGGTTAGAAAGCAGATGGCTCAG
ATTCGAGAATTAGTAGAACTTCCATTAAGGCACCCGCAGCTCTTTAAGTC
AATTGGTGTGAAGCCACCGAAAGGAATTTTGCTTTATGGACCTCCTGGAT
CTTGGGAAGACACTGATTGCCCGTGCTGTTGCAAATGAAACCGGTGCTTT
CTTCTTTTGTATAAATGGACCTGAGATCATGTCAAAA