CF504529

Overview
NameCF504529
Unique NameCF504529
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length473
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: ATG8_YEAST (Autophagy-related protein 8 OS=Saccharomyces cerevisiae GN=ATG8 PE=1 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 1.886e-40
Identity = 77/102 (75.49%), Postives = 92/102 (90.20%), Query Frame = 3 
Query:   54 RPESARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFG 359
            + ES RI +++ +RIPVI EKAE+SDIP+IDK+KYLVPADLTVGQFVYV+RKRI L  EKAIFIFV + LPPTAA+MSAIY+E+KD+DGFLY+TYSGENTFG
Sbjct:   15 KAESERIADRFKNRIPVICEKAEKSDIPEIDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHKDKDGFLYVTYSGENTFG 116

HSP 2 Score: 23.8682 bits (50), Expect = 1.886e-40
Identity = 9/15 (60.00%), Postives = 13/15 (86.67%), Query Frame = 2 
Query:   14 KSSFKLEHPLERRQA 58
            KS+FK E+P E+R+A
Sbjct:    2 KSTFKSEYPFEKRKA 16
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: ATG8_YEAS7 (Autophagy-related protein 8 OS=Saccharomyces cerevisiae (strain YJM789) GN=ATG8 PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 1.886e-40
Identity = 77/102 (75.49%), Postives = 92/102 (90.20%), Query Frame = 3 
Query:   54 RPESARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFG 359
            + ES RI +++ +RIPVI EKAE+SDIP+IDK+KYLVPADLTVGQFVYV+RKRI L  EKAIFIFV + LPPTAA+MSAIY+E+KD+DGFLY+TYSGENTFG
Sbjct:   15 KAESERIADRFKNRIPVICEKAEKSDIPEIDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHKDKDGFLYVTYSGENTFG 116

HSP 2 Score: 23.8682 bits (50), Expect = 1.886e-40
Identity = 9/15 (60.00%), Postives = 13/15 (86.67%), Query Frame = 2 
Query:   14 KSSFKLEHPLERRQA 58
            KS+FK E+P E+R+A
Sbjct:    2 KSTFKSEYPFEKRKA 16
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: ATG8_CANGA (Autophagy-related protein 8 OS=Candida glabrata GN=ATG8 PE=3 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 2.456e-40
Identity = 75/102 (73.53%), Postives = 91/102 (89.22%), Query Frame = 3 
Query:   54 RPESARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFG 359
            + ES RI EK+ +RIPVI EKAE+SDIP++DK+KYLVPADLTVGQFVYV+RKRI L  EKAIFIFV + LPPTA++MS +Y+E+KD+DGFLY+TYSGENTFG
Sbjct:   15 KAESERISEKFQNRIPVICEKAEKSDIPEVDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTASLMSQVYQEHKDKDGFLYVTYSGENTFG 116

HSP 2 Score: 25.0238 bits (53), Expect = 2.456e-40
Identity = 10/15 (66.67%), Postives = 13/15 (86.67%), Query Frame = 2 
Query:   14 KSSFKLEHPLERRQA 58
            KSSFK E+P E+R+A
Sbjct:    2 KSSFKSEYPFEKRKA 16
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: ATG8_PICAN (Autophagy-related protein 8 OS=Pichia angusta GN=ATG8 PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 3.197e-40
Identity = 76/102 (74.51%), Postives = 91/102 (89.22%), Query Frame = 3 
Query:   54 RPESARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFG 359
            + E++RIR K+ DRIPVI EK E SDIP+IDK+KYLVP+DLTVGQFVYV+RKRI+L +EKAIFIFV +ILPPTA++MS IYE+ KDEDGFLY+ YSGENTFG
Sbjct:   15 KAEASRIRGKFLDRIPVICEKVEESDIPEIDKRKYLVPSDLTVGQFVYVIRKRIQLPSEKAIFIFVNDILPPTASLMSTIYEQYKDEDGFLYILYSGENTFG 116

HSP 2 Score: 25.0238 bits (53), Expect = 3.197e-40
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 2 
Query:   14 KSSFKLEHPLERRQA 58
            +S FK EHP ERR+A
Sbjct:    2 RSQFKDEHPFERRKA 16
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: ATG8_PICST (Autophagy-related protein 8 OS=Pichia stipitis GN=ATG8 PE=3 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 4.157e-40
Identity = 74/100 (74.00%), Postives = 89/100 (89.00%), Query Frame = 3 
Query:   60 ESARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFG 359
            E+ RI +++ DR+PVI EK E SDIP+IDK+KYLVP DL+VGQFVYV+RKRIKL +EKAIFIFV +ILPPTAA++S IYEE+KDEDGFLY+ YSGENTFG
Sbjct:   17 EATRIAQRFKDRVPVICEKVENSDIPEIDKRKYLVPVDLSVGQFVYVIRKRIKLPSEKAIFIFVNDILPPTAALISTIYEEHKDEDGFLYVLYSGENTFG 116

HSP 2 Score: 25.409 bits (54), Expect = 4.157e-40
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 2 
Query:   14 KSSFKLEHPLERRQA 58
            +S FK EHP E+RQA
Sbjct:    2 RSQFKDEHPFEKRQA 16
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: ATG8_DEBHA (Autophagy-related protein 8 OS=Debaryomyces hansenii GN=ATG8 PE=3 SV=2)

HSP 1 Score: 158.303 bits (399), Expect = 2.035e-39
Identity = 74/102 (72.55%), Postives = 90/102 (88.24%), Query Frame = 3 
Query:   54 RPESARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFG 359
            + E+ RI +++ DR+PVI EKAE SDI +IDK+KYLVP DL+VGQFVYV+RKRIKL +EKAIFIF+ +ILPPTAA+MS IYEE+KDEDGFLY+ YSGENTFG
Sbjct:   15 KAEATRITQRFNDRLPVICEKAENSDIQEIDKRKYLVPGDLSVGQFVYVIRKRIKLPSEKAIFIFINDILPPTAALMSTIYEEHKDEDGFLYVLYSGENTFG 116

HSP 2 Score: 23.8682 bits (50), Expect = 2.035e-39
Identity = 9/15 (60.00%), Postives = 12/15 (80.00%), Query Frame = 2 
Query:   14 KSSFKLEHPLERRQA 58
            +S FK EHP E+R+A
Sbjct:    2 RSQFKDEHPFEKRKA 16
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: ATG8_DICDI (Autophagy-related protein 8 OS=Dictyostelium discoideum GN=atg8 PE=1 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 2.874e-38
Identity = 80/112 (71.43%), Postives = 92/112 (82.14%), Query Frame = 3 
Query:   33 NTHSKGGRPESA-RIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRI-KLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFGA 362
            N H    R E A RIR KY DRIPVIVEKA RSD PDIDKKKYLVPAD+TVG+FVY +RK + K+SAEKAI++FV N +PPTAA++S IYE  KDEDGFLY+TYSGENTFG+
Sbjct:    9 NDHPLDKRREVAERIRSKYLDRIPVIVEKAPRSDAPDIDKKKYLVPADITVGKFVYEIRKHMTKVSAEKAIYLFVNNTIPPTAALISQIYERYKDEDGFLYITYSGENTFGS 120

HSP 2 Score: 22.7126 bits (47), Expect = 2.874e-38
Identity = 8/13 (61.54%), Postives = 12/13 (92.31%), Query Frame = 2 
Query:   17 SSFKLEHPLERRQ 55
            SSFK +HPL++R+
Sbjct:    5 SSFKNDHPLDKRR 17
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: ATG8_ASHGO (Autophagy-related protein 8 OS=Ashbya gossypii GN=ATG8 PE=3 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 2.876e-38
Identity = 71/103 (68.93%), Postives = 92/103 (89.32%), Query Frame = 3 
Query:   54 RPESARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFGA 362
            + ES RI  ++ +R+PVI E+AE+SDIP++D++KYLVPADLTVGQFVYV+R+RIKL AEKAIFIFV + LPPTAA+M A+Y+E+KD+DGFLY+ YSGENTFG+
Sbjct:   14 KAESERICSQFENRVPVICERAEKSDIPEVDRRKYLVPADLTVGQFVYVIRRRIKLPAEKAIFIFVNDTLPPTAALMFAVYQEHKDKDGFLYVKYSGENTFGS 116

HSP 2 Score: 23.0978 bits (48), Expect = 2.876e-38
Identity = 9/14 (64.29%), Postives = 12/14 (85.71%), Query Frame = 2 
Query:   17 SSFKLEHPLERRQA 58
            SSFK E+P E+R+A
Sbjct:    2 SSFKSEYPFEKRKA 15
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: ATG8_KLULA (Autophagy-related protein 8 OS=Kluyveromyces lactis GN=ATG8 PE=3 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 6.352e-38
Identity = 73/102 (71.57%), Postives = 90/102 (88.24%), Query Frame = 3 
Query:   54 RPESARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFG 359
            + ES RI +K+ +RIPVI E+  +SDIPDIDK+KYLVP DLTVGQFVYV+RKRIKL AEKAIFIFV + LPPTAA+MS+IY+++KD+DGFLY++YS ENTFG
Sbjct:   15 KAESERIVQKFHNRIPVICERGGKSDIPDIDKRKYLVPGDLTVGQFVYVIRKRIKLPAEKAIFIFVNDTLPPTAALMSSIYQQHKDKDGFLYVSYSSENTFG 116

HSP 2 Score: 22.7126 bits (47), Expect = 6.352e-38
Identity = 9/15 (60.00%), Postives = 12/15 (80.00%), Query Frame = 2 
Query:   14 KSSFKLEHPLERRQA 58
            KS+FK E P E+R+A
Sbjct:    2 KSAFKSEFPFEKRKA 16
[Close]
BLAST of CF504529 vs. ExPASy Swiss-Prot
Match: GBRL2_RAT (Gamma-aminobutyric acid receptor-associated protein-like 2 OS=Rattus norvegicus GN=Gabarapl2 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 4.380e-33
Identity = 64/100 (64.00%), Postives = 82/100 (82.00%), Query Frame = 3 
Query:   60 ESARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFG 359
            ESA+IR KYPDR+PVIVEK   S I DIDK+KYLVP+D+TV QF++++RKRI+L +EKAIF+FV   +P ++  M  +YE+ KDEDGFLY+ YSGENTFG
Sbjct:   17 ESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGFLYVAYSGENTFG 116
The following BLAST results are available for this feature:
BLAST of CF504529 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 83
Match NameE-valueIdentityDescription
ATG8_YEAST1.886e-4075.49Autophagy-related protein 8 OS=Saccharomyces cerev... [more]
ATG8_YEAS71.886e-4075.49Autophagy-related protein 8 OS=Saccharomyces cerev... [more]
ATG8_CANGA2.456e-4073.53Autophagy-related protein 8 OS=Candida glabrata GN... [more]
ATG8_PICAN3.197e-4074.51Autophagy-related protein 8 OS=Pichia angusta GN=A... [more]
ATG8_PICST4.157e-4074.00Autophagy-related protein 8 OS=Pichia stipitis GN=... [more]
ATG8_DEBHA2.035e-3972.55Autophagy-related protein 8 OS=Debaryomyces hansen... [more]
ATG8_DICDI2.874e-3871.43Autophagy-related protein 8 OS=Dictyostelium disco... [more]
ATG8_ASHGO2.876e-3868.93Autophagy-related protein 8 OS=Ashbya gossypii GN=... [more]
ATG8_KLULA6.352e-3871.57Autophagy-related protein 8 OS=Kluyveromyces lacti... [more]
GBRL2_RAT4.380e-3364.00Gamma-aminobutyric acid receptor-associated protei... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-018 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-19_D02 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504529 ID=CF504529; Name=CF504529; organism=Citrus sinensis; type=EST; length=473bp
AGAAGCTATGGCCAAGAGCTCCTTCAAGCTTGAACACCCACTCGAAAGGA
GGCAGGCCTGAATCTGCCCGTATCAGGGAGAAGTATCCTGATAGAATACC
AGTGATTGTGGAGAAGGCTGAAAGGAGTGACATACCTGATATTGACAAAA
AAAAATACCTGGTTCCTGCTGATCTGACTGTTGGACAATTTGTTTATGTG
GTCCGGAAGAGGATAAAGCTCAGTGCTGAGAAGGCCATATTCATATTTGT
CAAGAACATTTTGCCACCCACTGCTGCAATGATGTCTGCAATTTATGAGG
AAAACAAAGATGAGGATGGCTTCCTTTACATGACTTACAGTGGGGAGAAC
ACATTCGGGGCATCTTTCTAAGGGAATCAACTGAACTTCTGTAGATTAAC
TCTGAAAAGACTTGATGGAAATATGTAAATCCTTTGCTATTTGCTTTCAA
CTGAGGGAGCACTTGGAAAGCAT
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