CF504605

Overview
NameCF504605
Unique NameCF504605
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length431
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_BACSU (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus subtilis GN=pckA PE=3 SV=3)

HSP 1 Score: 130.568 bits (327), Expect = 2.990e-38
Identity = 63/102 (61.76%), Postives = 73/102 (71.57%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I LS EKEP I+NAI+FG+VLENVV DE TRE +Y D   TENTRAAYPI  I N   P +  HP  ++ L  DAFGVLPP+SKL   Q MYHF+SGYT+ L
Sbjct:  275 IHLSEEKEPQIFNAIRFGSVLENVVVDEDTREANYDDSFYTENTRAAYPIHMINNIVTPSMAGHPSAIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKL 376

HSP 2 Score: 47.7506 bits (112), Expect = 2.990e-38
Identity = 21/42 (50.00%), Postives = 27/42 (64.29%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE G+  P  TFS CFG+ F+ L    YA ML +K+ +HG
Sbjct:  377 AGTERGVTSPETTFSTCFGSPFLPLPAHVYAEMLGKKIDEHG 418
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_FUSNN (Phosphoenolpyruvate carboxykinase [ATP] OS=Fusobacterium nucleatum subsp. nucleatum GN=pckA PE=3 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 3.895e-38
Identity = 57/102 (55.88%), Postives = 76/102 (74.51%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I+L  E EP+I+ AIKFG+++ENVV D  TR++ Y D S+T NTR  YPI+YIPNA++  VG  PK VI L  D+FGVLPP+S+L+    MYHF++G+TA L
Sbjct:  270 INLKEESEPEIYRAIKFGSLVENVVVDPITRKIQYEDASITPNTRVGYPIDYIPNAELSGVGGIPKVVIFLTADSFGVLPPISRLSQEAAMYHFVTGFTAKL 371

HSP 2 Score: 54.299 bits (129), Expect = 3.895e-38
Identity = 23/41 (56.10%), Postives = 30/41 (73.17%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+KEP  TFS CFG  F+ + P+ YA ML E+++KH
Sbjct:  372 AGTELGVKEPVPTFSTCFGEPFMPMDPSVYAEMLGERLKKH 412
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_SILPO (Phosphoenolpyruvate carboxykinase [ATP] OS=Silicibacter pomeroyi GN=pckA PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 5.074e-38
Identity = 57/101 (56.44%), Postives = 78/101 (77.23%), Query Frame = 3 
Query:    3 IDLSREKEPDIW-NAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+L+ E EP+I+    KF  V+EN+VFDE T+E+D+ D S+T N R AYP+EYI NA +  +G HPKN+I+L CDAFGVLPP+++L  AQ MYHF+SG+T+
Sbjct:  279 INLNPEAEPEIYATTTKFATVIENMVFDEETKELDFDDDSLTANMRCAYPLEYISNASVSALGGHPKNIIMLTCDAFGVLPPIARLTPAQAMYHFLSGFTS 379

HSP 2 Score: 48.9062 bits (115), Expect = 5.074e-38
Identity = 21/42 (50.00%), Postives = 27/42 (64.29%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE G+ EP  TFS CFGA F+   P  Y  +L +K+ +HG
Sbjct:  382 AGTERGVTEPQPTFSTCFGAPFMPRRPEVYGNLLRDKIARHG 423
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_SULDN (Phosphoenolpyruvate carboxykinase [ATP] OS=Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) GN=pckA PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 5.074e-38
Identity = 58/100 (58.00%), Postives = 74/100 (74.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+L    EP+I+NAIK GA+LENVV ++   EVDY+D S TENTR +YPIE+I N +      HP+N+I L+ DAFG+LPPVSKL   Q MY+F+SGYTA
Sbjct:  273 INLDGNNEPEIFNAIKSGALLENVVVNDKG-EVDYNDGSKTENTRVSYPIEHIKNHETSLSAGHPENIIFLSADAFGILPPVSKLTREQAMYYFLSGYTA 371

HSP 2 Score: 61.2326 bits (147), Expect = 5.074e-38
Identity = 27/41 (65.85%), Postives = 32/41 (78.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE GI +P ATFS+CFG AF+ LHPT YA +L EK+ KH
Sbjct:  374 AGTERGITKPVATFSSCFGEAFLPLHPTVYAKLLGEKIDKH 414
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_GEOTN (Phosphoenolpyruvate carboxykinase [ATP] OS=Geobacillus thermodenitrificans (strain NG80-2) GN=pckA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 6.610e-38
Identity = 61/102 (59.80%), Postives = 75/102 (73.53%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            I+LSREKEP I++AI FGAVLENV+  + TR  DY D ++TENTRAAYP++ I N   P V  HP  ++ L  DAFGVLPP+SKL   Q MYHF+SGYT+ L
Sbjct:  275 INLSREKEPQIFDAIGFGAVLENVILHDATRVPDYDDGTLTENTRAAYPLQAIKNIVDPSVAGHPSTIVFLTADAFGVLPPISKLTREQAMYHFLSGYTSKL 376

HSP 2 Score: 49.6766 bits (117), Expect = 6.610e-38
Identity = 22/41 (53.66%), Postives = 28/41 (68.29%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+ EP ATFS CFGA F+     +YA ML +K+ +H
Sbjct:  377 AGTERGVTEPEATFSTCFGAPFLPRPAVEYAEMLGQKIAEH 417
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_RHOS4 (Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) GN=pckA PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 8.611e-38
Identity = 58/101 (57.43%), Postives = 78/101 (77.23%), Query Frame = 3 
Query:    3 IDLSREKEPDIW-NAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+LS E EP+I+    KF  V+EN+V+DE T E+D++D S+T NTR AYP++YI NA    +G HPKNVI+L CDAFGVLPP+++L  AQ MYHF+SG+T+
Sbjct:  279 INLSAEAEPEIYATTSKFATVVENMVYDEETLELDFNDDSLTANTRCAYPLDYISNASESGLGGHPKNVIMLTCDAFGVLPPIARLTPAQAMYHFLSGFTS 379

HSP 2 Score: 51.6026 bits (122), Expect = 8.611e-38
Identity = 23/42 (54.76%), Postives = 27/42 (64.29%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE G+ EP  TFS CFGA F+   P  Y  +L EK+ KHG
Sbjct:  382 AGTERGVTEPQPTFSTCFGAPFMPRRPEVYGKLLQEKIAKHG 423
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_RHOS1 (Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) GN=pckA PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 8.611e-38
Identity = 58/101 (57.43%), Postives = 78/101 (77.23%), Query Frame = 3 
Query:    3 IDLSREKEPDIW-NAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+LS E EP+I+    KF  V+EN+V+DE T E+D++D S+T NTR AYP++YI NA    +G HPKNVI+L CDAFGVLPP+++L  AQ MYHF+SG+T+
Sbjct:  279 INLSAEAEPEIYATTSKFATVVENMVYDEETLELDFNDDSLTANTRCAYPLDYISNASESGLGGHPKNVIMLTCDAFGVLPPIARLTPAQAMYHFLSGFTS 379

HSP 2 Score: 51.6026 bits (122), Expect = 8.611e-38
Identity = 23/42 (54.76%), Postives = 27/42 (64.29%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTE G+ EP  TFS CFGA F+   P  Y  +L EK+ KHG
Sbjct:  382 AGTERGVTEPQPTFSTCFGAPFMPRRPEVYGKLLQEKIAKHG 423
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_GRAFK (Phosphoenolpyruvate carboxykinase [ATP] OS=Gramella forsetii (strain KT0803) GN=pckA PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.122e-37
Identity = 57/100 (57.00%), Postives = 72/100 (72.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+LS E EPDI+ AIK GA+LENV  D +  +VD+ D ++T+NTR +YPI +I N + P  G +PKN+  L  DAFGVLPP+SKL   Q  YHFISGYTA
Sbjct:  280 INLSEENEPDIYKAIKPGAILENVALDANG-DVDFEDVTITQNTRVSYPINHINNIQKPSTGENPKNIFFLTADAFGVLPPISKLTPGQAAYHFISGYTA 378

HSP 2 Score: 57.7658 bits (138), Expect = 1.122e-37
Identity = 25/39 (64.10%), Postives = 32/39 (82.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQ 421
            AGTE G+ EP  +FSACFGA F+ LHPT+YA ML++KM+
Sbjct:  381 AGTEAGVDEPVPSFSACFGAPFMPLHPTEYAEMLSKKMK 419
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_LEPBP (Phosphoenolpyruvate carboxykinase [ATP] OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) GN=pckA PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.122e-37
Identity = 54/100 (54.00%), Postives = 71/100 (71.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+L  + EP+I+ AI+  A+LENVV+D  T++VDYS  + TENTR +YPI +I N +      HP  VI L  DA+GVLP VSKL++ Q MYHF+SGYTA
Sbjct:  276 INLDPKTEPEIYAAIRRDALLENVVYDATTKKVDYSSAAKTENTRVSYPIFHIDNIQPGSKAGHPNTVIFLTYDAYGVLPAVSKLSIEQAMYHFLSGYTA 375

HSP 2 Score: 67.3958 bits (163), Expect = 1.122e-37
Identity = 30/41 (73.17%), Postives = 34/41 (82.93%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+KEP ATFSACFG AF+ LHPT YA +L EKM+KH
Sbjct:  378 AGTERGVKEPQATFSACFGQAFMTLHPTYYAKLLGEKMKKH 418
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_LEPBA (Phosphoenolpyruvate carboxykinase [ATP] OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames) GN=pckA PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.122e-37
Identity = 54/100 (54.00%), Postives = 71/100 (71.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+L  + EP+I+ AI+  A+LENVV+D  T++VDYS  + TENTR +YPI +I N +      HP  VI L  DA+GVLP VSKL++ Q MYHF+SGYTA
Sbjct:  276 INLDPKTEPEIYAAIRRDALLENVVYDATTKKVDYSSAAKTENTRVSYPIFHIDNIQPGSKAGHPNTVIFLTYDAYGVLPAVSKLSIEQAMYHFLSGYTA 375

HSP 2 Score: 67.3958 bits (163), Expect = 1.122e-37
Identity = 30/41 (73.17%), Postives = 34/41 (82.93%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+KEP ATFSACFG AF+ LHPT YA +L EKM+KH
Sbjct:  378 AGTERGVKEPQATFSACFGQAFMTLHPTYYAKLLGEKMKKH 418
The following BLAST results are available for this feature:
BLAST of CF504605 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284
Match NameE-valueIdentityDescription
PCKA_BACSU2.990e-3861.76Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu... [more]
PCKA_FUSNN3.895e-3855.88Phosphoenolpyruvate carboxykinase [ATP] OS=Fusobac... [more]
PCKA_SILPO5.074e-3856.44Phosphoenolpyruvate carboxykinase [ATP] OS=Silicib... [more]
PCKA_SULDN5.074e-3858.00Phosphoenolpyruvate carboxykinase [ATP] OS=Sulfuri... [more]
PCKA_GEOTN6.610e-3859.80Phosphoenolpyruvate carboxykinase [ATP] OS=Geobaci... [more]
PCKA_RHOS48.611e-3857.43Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodoba... [more]
PCKA_RHOS18.611e-3857.43Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodoba... [more]
PCKA_GRAFK1.122e-3757.00Phosphoenolpyruvate carboxykinase [ATP] OS=Gramell... [more]
PCKA_LEPBP1.122e-3754.00Phosphoenolpyruvate carboxykinase [ATP] OS=Leptosp... [more]
PCKA_LEPBA1.122e-3754.00Phosphoenolpyruvate carboxykinase [ATP] OS=Leptosp... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-094 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-1_C03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504605 ID=CF504605; Name=CF504605; organism=Citrus sinensis; type=EST; length=431bp
GCATTGATCTCTCAAGGGAGAAGGAGCCTGATATCTGGAATGCCATCAAG
TTTGGCGCTGTGTTGGAAAATGTCGTGTTTGATGAGCATACCAGAGAGGT
GGATTATTCTGACAAATCTGTCACAGAGAACACTCGTGCTGCCTACCCCA
TTGAGTATATCCCCAATGCAAAGATACCATGCGTTGGTCCTCATCCCAAG
AATGTCATCCTTTTGGCATGTGATGCTTTTGGTGTACTCCCACCAGTGAG
CAAGCTGAACCTGGCACAAACAATGTACCATTTCATCAGTGGCTACACTG
CTTTGCTGGAACAGAGGATGGTATTAAGGAGCCAACGGCAACATTTTCAG
CTTGCTTTGGTGCAGCATTTATCATGCTGCATCCTACCAAGTATGCAGCA
ATGCTGGCTGAGAAGATGCAGAAGCATGGTG
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