CF504653 | Citrus Genome Database

Overview


Name	CF504653
Unique Name	CF504653
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	331

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: MK07_MOUSE (Mitogen-activated protein kinase 7 OS=Mus musculus GN=Mapk7 PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.227e-20
Identity = 36/72 (50.00%), Postives = 52/72 (72.22%), Query Frame = 3

Query:    6 TGEKVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPC-RREFKDIYVVFELMESDLDQVI 218
            TG++VAIKK+   F+ V++A R LRE+K+L+  KH +I+ IK I+ P     EF+ +YVV +LMESDL Q+I
Sbjct:   77 TGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILKPTVPYGEFRSVYVVLDLMESDLHQII 148

HSP 2 Score: 47.7506 bits (112), Expect = 1.227e-20
Identity = 20/32 (62.50%), Postives = 25/32 (78.12%), Query Frame = 2

Query:  215 YKANVFHRDLKPKNILANADCKLKICDFGLAR 310
            + A V HRDLKP N+L N +C+LKI DFG+AR
Sbjct:  174 HSAQVIHRDLKPSNLLVNENCELKIGDFGMAR 205

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: NLK_MOUSE (Serine/threonine protein kinase NLK OS=Mus musculus GN=Nlk PE=1 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 2.721e-20
Identity = 30/70 (42.86%), Postives = 48/70 (68.57%), Query Frame = 3

Query:    9 GEKVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPCRREFKDIYVVFELMESDLDQVI 218
            G++VA+KKM  VF+++    R+ RE+K+L   KH +++    I+ PP    F++IYVV ELM+SDL ++I
Sbjct:  161 GKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKII 230

HSP 2 Score: 53.1434 bits (126), Expect = 2.721e-20
Identity = 22/37 (59.46%), Postives = 28/37 (75.68%), Query Frame = 2

Query:  215 YKANVFHRDLKPKNILANADCKLKICDFGLARVSFTD 325
            + A + HRD+KP N+L N++C LKICDFGLARV   D
Sbjct:  256 HSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELD 292

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: NLK_HUMAN (Serine/threonine protein kinase NLK OS=Homo sapiens GN=NLK PE=1 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 2.721e-20
Identity = 30/70 (42.86%), Postives = 48/70 (68.57%), Query Frame = 3

Query:    9 GEKVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPCRREFKDIYVVFELMESDLDQVI 218
            G++VA+KKM  VF+++    R+ RE+K+L   KH +++    I+ PP    F++IYVV ELM+SDL ++I
Sbjct:  161 GKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKII 230

HSP 2 Score: 53.1434 bits (126), Expect = 2.721e-20
Identity = 22/37 (59.46%), Postives = 28/37 (75.68%), Query Frame = 2

Query:  215 YKANVFHRDLKPKNILANADCKLKICDFGLARVSFTD 325
            + A + HRD+KP N+L N++C LKICDFGLARV   D
Sbjct:  256 HSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELD 292

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: NLK2_XENTR (Serine/threonine protein kinase NLK2 OS=Xenopus tropicalis GN=nlk.2 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.016e-19
Identity = 29/70 (41.43%), Postives = 48/70 (68.57%), Query Frame = 3

Query:    9 GEKVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPCRREFKDIYVVFELMESDLDQVI 218
            G++VA+KKM  VF+++    R+ RE+K+L   KH +++    I+ PP    F++IYV+ ELM++DL +VI
Sbjct:   90 GKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPQIDCFEEIYVITELMQTDLHKVI 159

HSP 2 Score: 53.1434 bits (126), Expect = 1.016e-19
Identity = 22/37 (59.46%), Postives = 28/37 (75.68%), Query Frame = 2

Query:  215 YKANVFHRDLKPKNILANADCKLKICDFGLARVSFTD 325
            + A + HRD+KP N+L N++C LKICDFGLARV   D
Sbjct:  185 HSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELD 221

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: NLK2_XENLA (Serine/threonine protein kinase NLK2 OS=Xenopus laevis GN=nlk.2 PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.016e-19
Identity = 29/70 (41.43%), Postives = 48/70 (68.57%), Query Frame = 3

Query:    9 GEKVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPCRREFKDIYVVFELMESDLDQVI 218
            G++VA+KKM  VF+++    R+ RE+K+L   KH +++    I+ PP    F++IYV+ ELM++DL +VI
Sbjct:   83 GKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPQIDCFEEIYVITELMQTDLHKVI 152

HSP 2 Score: 53.1434 bits (126), Expect = 1.016e-19
Identity = 22/37 (59.46%), Postives = 28/37 (75.68%), Query Frame = 2

Query:  215 YKANVFHRDLKPKNILANADCKLKICDFGLARVSFTD 325
            + A + HRD+KP N+L N++C LKICDFGLARV   D
Sbjct:  178 HSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELD 214

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: SPK1_SCHPO (Mitogen-activated protein kinase spk1 OS=Schizosaccharomyces pombe GN=spk1 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.019e-19
Identity = 35/72 (48.61%), Postives = 49/72 (68.06%), Query Frame = 3

Query:    6 TGEKVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPCRREFKDIYVVFELMESDLDQVIK 221
            +G KVA+KK+   F H     R LREIKLLR  +H +I+ I  I+ PP  +E +D+Y+V ELME+DL +VI+
Sbjct:   61 SGLKVAVKKIHP-FNHPVFCLRTLREIKLLRHFRHENIISILDILPPPSYQELEDVYIVQELMETDLYRVIR 131

HSP 2 Score: 50.447 bits (119), Expect = 1.019e-19
Identity = 21/35 (60.00%), Postives = 27/35 (77.14%), Query Frame = 2

Query:  206 RSSYKANVFHRDLKPKNILANADCKLKICDFGLAR 310
            ++ + A V HRDLKP N+L NA+C LK+ DFGLAR
Sbjct:  152 KAMHSAGVVHRDLKPSNLLLNANCDLKVADFGLAR 186

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: MK01_XENLA (Mitogen-activated protein kinase 1 OS=Xenopus laevis GN=mapk1 PE=1 SV=3)

HSP 1 Score: 59.3066 bits (142), Expect = 1.020e-19
Identity = 31/69 (44.93%), Postives = 46/69 (66.67%), Query Frame = 3

Query:   15 KVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPCRREFKDIYVVFELMESDLDQVIK 221
            +VAIKK+   FEH +   R LREIK+L   KH +I+ I  I+  P   + KD+Y+V +LME+DL +++K
Sbjct:   53 RVAIKKISP-FEHQTYCQRTLREIKILLRFKHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLK 120

HSP 2 Score: 56.6102 bits (135), Expect = 1.020e-19
Identity = 25/37 (67.57%), Postives = 28/37 (75.68%), Query Frame = 2

Query:  215 YKANVFHRDLKPKNILANADCKLKICDFGLARVSFTD 325
            + ANV HRDLKP N+L N  C LKICDFGLARV+  D
Sbjct:  144 HSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPD 180

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: NLK_CAEEL (Serine/threonine kinase NLK OS=Caenorhabditis elegans GN=lit-1 PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.707e-19
Identity = 27/71 (38.03%), Postives = 50/71 (70.42%), Query Frame = 3

Query:    6 TGEKVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPCRREFKDIYVVFELMESDLDQVI 218
            +G++VA+KKM  VF++++   R+ REIK+L   +H +++ +  I+ P     F+++YV+ ELM+SDL ++I
Sbjct:  262 SGKRVALKKMPNVFQNLASCKRVFREIKMLSSFRHDNVLSLLDILQPANPSFFQELYVLTELMQSDLHKII 332

HSP 2 Score: 52.373 bits (124), Expect = 1.707e-19
Identity = 21/32 (65.62%), Postives = 27/32 (84.38%), Query Frame = 2

Query:  215 YKANVFHRDLKPKNILANADCKLKICDFGLAR 310
            + AN+ HRD+KP N+L N++C LKICDFGLAR
Sbjct:  358 HTANILHRDIKPGNLLVNSNCILKICDFGLAR 389

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: MK01_HUMAN (Mitogen-activated protein kinase 1 OS=Homo sapiens GN=MAPK1 PE=1 SV=3)

HSP 1 Score: 58.151 bits (139), Expect = 2.244e-19
Identity = 30/69 (43.48%), Postives = 46/69 (66.67%), Query Frame = 3

Query:   15 KVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPCRREFKDIYVVFELMESDLDQVIK 221
            +VAIKK+   FEH +   R LREIK+L   +H +I+ I  I+  P   + KD+Y+V +LME+DL +++K
Sbjct:   50 RVAIKKISP-FEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLK 117

HSP 2 Score: 56.6102 bits (135), Expect = 2.244e-19
Identity = 25/37 (67.57%), Postives = 28/37 (75.68%), Query Frame = 2

Query:  215 YKANVFHRDLKPKNILANADCKLKICDFGLARVSFTD 325
            + ANV HRDLKP N+L N  C LKICDFGLARV+  D
Sbjct:  141 HSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPD 177

[Close]

BLAST of CF504653 vs. ExPASy Swiss-Prot
Match: MK01_BOVIN (Mitogen-activated protein kinase 1 OS=Bos taurus GN=MAPK1 PE=2 SV=3)

HSP 1 Score: 58.151 bits (139), Expect = 2.244e-19
Identity = 30/69 (43.48%), Postives = 46/69 (66.67%), Query Frame = 3

Query:   15 KVAIKKMKEVFEHVSDATRILREIKLLRMLKHPDIVEIKHIMLPPCRREFKDIYVVFELMESDLDQVIK 221
            +VAIKK+   FEH +   R LREIK+L   +H +I+ I  I+  P   + KD+Y+V +LME+DL +++K
Sbjct:   50 RVAIKKISP-FEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLK 117

HSP 2 Score: 56.6102 bits (135), Expect = 2.244e-19
Identity = 25/37 (67.57%), Postives = 28/37 (75.68%), Query Frame = 2

Query:  215 YKANVFHRDLKPKNILANADCKLKICDFGLARVSFTD 325
            + ANV HRDLKP N+L N  C LKICDFGLARV+  D
Sbjct:  141 HSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPD 177

The following BLAST results are available for this feature:

BLAST of CF504653 vs. ExPASy Swiss-Prot

BLAST of CF504653 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 197


Match Name	E-value	Identity	Description
MK07_MOUSE	1.227e-20	50.00	Mitogen-activated protein kinase 7 OS=Mus musculus...	[more]
NLK_MOUSE	2.721e-20	42.86	Serine/threonine protein kinase NLK OS=Mus musculu...	[more]
NLK_HUMAN	2.721e-20	42.86	Serine/threonine protein kinase NLK OS=Homo sapien...	[more]
NLK2_XENTR	1.016e-19	41.43	Serine/threonine protein kinase NLK2 OS=Xenopus tr...	[more]
NLK2_XENLA	1.016e-19	41.43	Serine/threonine protein kinase NLK2 OS=Xenopus la...	[more]
SPK1_SCHPO	1.019e-19	48.61	Mitogen-activated protein kinase spk1 OS=Schizosac...	[more]
MK01_XENLA	1.020e-19	44.93	Mitogen-activated protein kinase 1 OS=Xenopus laev...	[more]
NLK_CAEEL	1.707e-19	38.03	Serine/threonine kinase NLK OS=Caenorhabditis eleg...	[more]
MK01_HUMAN	2.244e-19	43.48	Mitogen-activated protein kinase 1 OS=Homo sapiens...	[more]
MK01_BOVIN	2.244e-19	43.48	Mitogen-activated protein kinase 1 OS=Bos taurus G...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_120000-142 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-1_G08 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504653 ID=CF504653; Name=CF504653; organism=Citrus sinensis; type=EST; length=331bp
CTCATACTGGAGAGAAAGTTGCTATTAAGAAAATGAAAGAAGTATTTGAG
CATGTGTCGGATGCCACACGCATTTTAAGAGAGATCAAGCTCCTTCGGAT
GCTGAAACATCCCGACATTGTGGAAATCAAGCATATAATGCTTCCTCCAT
GTCGGAGAGAATTTAAAGATATTTATGTTGTTTTCGAGCTGATGGAATCT
GACCTCGATCAAGTTATAAAGCAAATGTTTTTCATCGTGATTTGAAGCCA
AAAAATATTCTCGCGAATGCGGATTGCAAACTGAAGATATGTGATTTTGG
CCTTGCACGTGTATCATTTACTGATACCCCT