CF509642 | Citrus Genome Database

Overview


Name	CF509642
Unique Name	CF509642
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	433

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_PIG (NADP-dependent malic enzyme (Fragment) OS=Sus scrofa GN=ME1 PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.291e-21
Identity = 33/53 (62.26%), Postives = 37/53 (69.81%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGT NE+LLKD  YIGLRQRR  G EY + LDEFM AV   YG   LIQ
Sbjct:  177 VILDVGTENEELLKDPLYIGLRQRRVRGPEYDDFLDEFMEAVSSKYGMNCLIQ 229

HSP 2 Score: 41.5874 bits (96), Expect = 2.291e-21
Identity = 19/28 (67.86%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFAN NAF LL KY   +  FNDDIQ
Sbjct:  230 FEDFANINAFRLLKKYQNQYCTFNDDIQ 257

HSP 3 Score: 31.187 bits (69), Expect = 2.291e-21
Identity = 14/29 (48.28%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L++   LF GAGEA  GIA LI + + K+
Sbjct:  278 LSDQTILFQGAGEAALGIAHLIVMAMEKE 306

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_COLLI (NADP-dependent malic enzyme OS=Columba livia GN=ME1 PE=1 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.970e-21
Identity = 32/53 (60.38%), Postives = 37/53 (69.81%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGT+NE LLKD  YIGLR +R  GQ Y +LLDEFM AV   YG   LIQ
Sbjct:  180 VMLDVGTDNETLLKDPLYIGLRHKRIRGQAYDDLLDEFMEAVTSRYGMNCLIQ 232

HSP 2 Score: 41.5874 bits (96), Expect = 2.970e-21
Identity = 19/28 (67.86%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFAN NAF LL KY   +  FNDDIQ
Sbjct:  233 FEDFANANAFRLLHKYRNKYCTFNDDIQ 260

HSP 3 Score: 33.8834 bits (76), Expect = 2.970e-21
Identity = 15/29 (51.72%), Postives = 20/29 (68.97%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L++H  LF GAGEA  GIA LI + + K+
Sbjct:  281 LSDHTVLFQGAGEAALGIANLIVMAMQKE 309

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_ANAPL (NADP-dependent malic enzyme OS=Anas platyrhynchos GN=ME1 PE=1 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.970e-21
Identity = 32/53 (60.38%), Postives = 37/53 (69.81%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGT+NE LLKD  YIGLR +R  GQ Y +LLDEFM AV   YG   LIQ
Sbjct:  180 VMLDVGTDNEALLKDPLYIGLRHKRIRGQAYDDLLDEFMEAVTSRYGMNCLIQ 232

HSP 2 Score: 41.5874 bits (96), Expect = 2.970e-21
Identity = 19/28 (67.86%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFAN NAF LL KY   +  FNDDIQ
Sbjct:  233 FEDFANANAFRLLHKYRNKYCTFNDDIQ 260

HSP 3 Score: 33.8834 bits (76), Expect = 2.970e-21
Identity = 15/29 (51.72%), Postives = 20/29 (68.97%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L++H  LF GAGEA  GIA LI + + K+
Sbjct:  281 LSDHTVLFQGAGEAALGIANLIVMAMEKE 309

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOM_HUMAN (NAD-dependent malic enzyme, mitochondrial OS=Homo sapiens GN=ME2 PE=1 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 8.371e-21
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGT+N  LLKD FY+GL Q+R   Q+Y +L+DEFM A+   YG   LIQ
Sbjct:  201 VCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQ 253

HSP 2 Score: 42.3578 bits (98), Expect = 8.371e-21
Identity = 18/28 (64.29%), Postives = 19/28 (67.86%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDF NHNAF  L KY   +  FNDDIQ
Sbjct:  254 FEDFGNHNAFRFLRKYREKYCTFNDDIQ 281

HSP 3 Score: 35.8094 bits (81), Expect = 8.371e-21
Identity = 15/26 (57.69%), Postives = 20/26 (76.92%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEI 379
            ++EH+ LFLGAGEA  GIA LI + +
Sbjct:  302 ISEHKILFLGAGEAALGIANLIVMSM 327

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_MOUSE (NADP-dependent malic enzyme OS=Mus musculus GN=Me1 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 8.377e-21
Identity = 33/53 (62.26%), Postives = 35/53 (66.04%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            IT+DVGT NE+LLKD  YIGLR RR  G EY   LDEFM A    YG   LIQ
Sbjct:  191 ITLDVGTENEELLKDPLYIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQ 243

HSP 2 Score: 42.3578 bits (98), Expect = 8.377e-21
Identity = 19/28 (67.86%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFAN NAF LL KY   +  FNDDIQ
Sbjct:  244 FEDFANRNAFRLLNKYRNKYCTFNDDIQ 271

HSP 3 Score: 30.4166 bits (67), Expect = 8.377e-21
Identity = 13/29 (44.83%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L++   LF GAGEA  GIA L+ + + K+
Sbjct:  292 LSDQTVLFQGAGEAALGIAHLVVMAMEKE 320

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAON_HUMAN (NADP-dependent malic enzyme, mitochondrial OS=Homo sapiens GN=ME3 PE=2 SV=2)

HSP 1 Score: 63.5438 bits (153), Expect = 1.084e-20
Identity = 29/53 (54.72%), Postives = 38/53 (71.70%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGTNNE+LL+D  YIGL+ +R  G+ Y +LLDEFM AV   +G   LIQ
Sbjct:  226 VLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQ 278

HSP 2 Score: 41.9726 bits (97), Expect = 1.084e-20
Identity = 19/28 (67.86%), Postives = 21/28 (75.00%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFAN NAF LL KY   + +FNDDIQ
Sbjct:  279 FEDFANANAFRLLNKYRNKYCMFNDDIQ 306

HSP 3 Score: 34.6538 bits (78), Expect = 1.084e-20
Identity = 16/37 (43.24%), Postives = 22/37 (59.46%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEET 412
            L+ H F+F GAGEA  GIA L+ + + K+     E T
Sbjct:  327 LSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEAT 363

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOM_MOUSE (NAD-dependent malic enzyme, mitochondrial OS=Mus musculus GN=Me2 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.085e-20
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGT+N  LLKD FY+GL Q+R   Q Y +L+DEFM A+   YG   LIQ
Sbjct:  201 VCIDVGTDNMALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQ 253

HSP 2 Score: 42.3578 bits (98), Expect = 1.085e-20
Identity = 18/28 (64.29%), Postives = 19/28 (67.86%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDF NHNAF  L KY   +  FNDDIQ
Sbjct:  254 FEDFGNHNAFRFLRKYQQKYCTFNDDIQ 281

HSP 3 Score: 36.1946 bits (82), Expect = 1.085e-20
Identity = 16/26 (61.54%), Postives = 20/26 (76.92%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEI 379
            ++EH+ LFLGAGEA  GIA LI L +
Sbjct:  302 VSEHKILFLGAGEAALGIANLIVLSM 327

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_RAT (NADP-dependent malic enzyme OS=Rattus norvegicus GN=Me1 PE=1 SV=2)

HSP 1 Score: 67.781 bits (164), Expect = 1.407e-20
Identity = 33/53 (62.26%), Postives = 35/53 (66.04%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            IT+DVGT NE+LLKD  YIGLR RR  G EY   LDEFM A    YG   LIQ
Sbjct:  191 ITLDVGTENEELLKDPLYIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQ 243

HSP 2 Score: 41.2022 bits (95), Expect = 1.407e-20
Identity = 19/28 (67.86%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFAN NAF LL KY   +  FNDDIQ
Sbjct:  244 FEDFANLNAFRLLNKYRNKYCTFNDDIQ 271

HSP 3 Score: 30.8018 bits (68), Expect = 1.407e-20
Identity = 14/29 (48.28%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L++   LF GAGEA  GIA LI + + K+
Sbjct:  292 LSDQTVLFQGAGEAALGIAHLIVMAMEKE 320

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAON_MOUSE (NADP-dependent malic enzyme, mitochondrial OS=Mus musculus GN=Me3 PE=1 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.820e-20
Identity = 30/53 (56.60%), Postives = 39/53 (73.58%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGTNNE+LL+D  YIGL+ +R  G+EY +LLDEFM AV   +G   LIQ
Sbjct:  226 VLLDVGTNNEELLRDPLYIGLKHQRVRGEEYDDLLDEFMQAVTDKFGINCLIQ 278

HSP 2 Score: 41.9726 bits (97), Expect = 1.820e-20
Identity = 19/28 (67.86%), Postives = 21/28 (75.00%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFAN NAF LL KY   + +FNDDIQ
Sbjct:  279 FEDFANANAFRLLNKYRNKYCMFNDDIQ 306

HSP 3 Score: 30.8018 bits (68), Expect = 1.820e-20
Identity = 13/29 (44.83%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+ H F+F GA EA  GIA L+ + + K+
Sbjct:  327 LSNHVFVFQGADEAAMGIAHLLVMALEKE 355

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOM_ASCSU (NAD-dependent malic enzyme, mitochondrial (Fragment) OS=Ascaris suum PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.354e-20
Identity = 30/53 (56.60%), Postives = 37/53 (69.81%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGTNN  LL D FYIGLR +R  G++Y  LLD FM A  + YG+K LIQ
Sbjct:  255 VLLDVGTNNMDLLNDPFYIGLRHKRVRGKDYDTLLDNFMKACTKKYGQKTLIQ 307

HSP 2 Score: 41.5874 bits (96), Expect = 2.354e-20
Identity = 19/28 (67.86%), Postives = 21/28 (75.00%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFAN NAF LL KY   + +FNDDIQ
Sbjct:  308 FEDFANPNAFRLLDKYQDKYTMFNDDIQ 335

HSP 3 Score: 31.5722 bits (70), Expect = 2.354e-20
Identity = 12/29 (41.38%), Postives = 21/29 (72.41%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            +++ ++LF GAG A TGIAE+I  ++  +
Sbjct:  356 VSQEKYLFFGAGAASTGIAEMIVHQMQNE 384

The following BLAST results are available for this feature:

BLAST of CF509642 vs. ExPASy Swiss-Prot

BLAST of CF509642 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 133


Match Name	E-value	Identity	Description
MAOX_PIG	2.291e-21	62.26	NADP-dependent malic enzyme (Fragment) OS=Sus scro...	[more]
MAOX_COLLI	2.970e-21	60.38	NADP-dependent malic enzyme OS=Columba livia GN=ME...	[more]
MAOX_ANAPL	2.970e-21	60.38	NADP-dependent malic enzyme OS=Anas platyrhynchos ...	[more]
MAOM_HUMAN	8.371e-21	52.83	NAD-dependent malic enzyme, mitochondrial OS=Homo ...	[more]
MAOX_MOUSE	8.377e-21	62.26	NADP-dependent malic enzyme OS=Mus musculus GN=Me1...	[more]
MAON_HUMAN	1.084e-20	54.72	NADP-dependent malic enzyme, mitochondrial OS=Homo...	[more]
MAOM_MOUSE	1.085e-20	52.83	NAD-dependent malic enzyme, mitochondrial OS=Mus m...	[more]
MAOX_RAT	1.407e-20	62.26	NADP-dependent malic enzyme OS=Rattus norvegicus G...	[more]
MAON_MOUSE	1.820e-20	56.60	NADP-dependent malic enzyme, mitochondrial OS=Mus ...	[more]
MAOM_ASCSU	2.354e-20	56.60	NAD-dependent malic enzyme, mitochondrial (Fragmen...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_125000-150 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-7_F07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF509642 ID=CF509642; Name=CF509642; organism=Citrus sinensis; type=EST; length=433bp
ATAACCGTTGATGTGGGTACAAACAATGAGCAATTGTTGAAGGATGAATT
CTACATTGGTCTACGACAGAGGAGGGCAACCGGACAGGAATATGCTGAAC
TTCTAGATGAATTCATGAGTGCTGTCAAGCAAAATTATGGAGAAAAAGTT
CTTATACAGGTTAGTTTGAGGATTTCGCAAACCACAATGCTTTTGAGTTG
CTTGCAAAATATGGCACAACTCATCTTGTCTTCAATGATGATATACAGCG
TCTGTTGTTCTTGCTGGGGTAGTTGCAGCACTGAAGTTGATTGGTGGCAC
ACTGGCCGAGCATAGATTTTTGTTTCTTGGAGCTGGGGAGGCTGGGACTG
GTATAGCTGAACTTATTGCGCTTGAGATATCAAAGCAGACTAAAGCTCCT
GTGGAAGAGACCCCGGAAGAAGATATGTCTTGT