CF509642 | Citrus Genome Database

Overview


Name	CF509642
Unique Name	CF509642
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	433

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SALDC (NAD-dependent malic enzyme OS=Salmonella dublin (strain CT_02021853) GN=sfcA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 5.791e-15
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFIQAVKQRWPDILL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.791e-15
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLTRYRDEICSFNDDIQ 272

HSP 3 Score: 32.3426 bits (72), Expect = 5.791e-15
Identity = 14/29 (48.28%), Postives = 20/29 (68.97%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE I  +  ++
Sbjct:  293 LSEQKIVFLGAGSAGCGIAEQIIAQTQRE 321

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SALCH (NAD-dependent malic enzyme OS=Salmonella choleraesuis GN=sfcA PE=3 SV=2)

HSP 1 Score: 54.6842 bits (130), Expect = 5.791e-15
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFIQAVKQRWPDILL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.791e-15
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLTRYRDEICSFNDDIQ 272

HSP 3 Score: 32.3426 bits (72), Expect = 5.791e-15
Identity = 14/29 (48.28%), Postives = 20/29 (68.97%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE I  +  ++
Sbjct:  293 LSEQKIVFLGAGSAGCGIAEQIIAQTQRE 321

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SALAR (NAD-dependent malic enzyme OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) GN=sfcA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 5.791e-15
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFIQAVKQRWPDILL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.791e-15
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLTRYRDEICSFNDDIQ 272

HSP 3 Score: 32.3426 bits (72), Expect = 5.791e-15
Identity = 14/29 (48.28%), Postives = 20/29 (68.97%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE I  +  ++
Sbjct:  293 LSEQKIVFLGAGSAGCGIAEQIIAQTQRE 321

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SALA4 (NAD-dependent malic enzyme OS=Salmonella agona (strain SL483) GN=sfcA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 5.791e-15
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+ AVKQ + + +L
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRHPRITDNEYYAFVDEFIQAVKQRWPDILL 243

HSP 2 Score: 33.4982 bits (75), Expect = 5.791e-15
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLTRYRDEICSFNDDIQ 272

HSP 3 Score: 32.3426 bits (72), Expect = 5.791e-15
Identity = 14/29 (48.28%), Postives = 20/29 (68.97%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L+E + +FLGAG AG GIAE I  +  ++
Sbjct:  293 LSEQKIVFLGAGSAGCGIAEQIIAQTQRE 321

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO12_PHOPR (NAD-dependent malic enzyme 2 OS=Photobacterium profundum GN=sfcA2 PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 7.496e-15
Identity = 25/46 (54.35%), Postives = 32/46 (69.57%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNY 138
            I +DVGTNN QLL D  Y+G R  R TGQEY + ++EF+ AVK  +
Sbjct:  190 IVLDVGTNNTQLLSDPMYMGWRHPRITGQEYDDFVEEFIQAVKSRW 235

HSP 2 Score: 33.113 bits (74), Expect = 7.496e-15
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  242 FEDFAQKNAMPLLNRYKDKVCCFNDDIQ 269

HSP 3 Score: 31.5722 bits (70), Expect = 7.496e-15
Identity = 14/26 (53.85%), Postives = 18/26 (69.23%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEI 379
            L+E R  F+GAG AG GIAE I  ++
Sbjct:  290 LSEQRITFVGAGSAGCGIAEAIVAQM 315

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_ERWT9 (NAD-dependent malic enzyme OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=sfcA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 9.693e-15
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGTNN+QLL D  Y+G R  R TG+EY   ++EF+ AVK  +   VL+Q
Sbjct:  193 VVLDVGTNNQQLLNDPLYMGWRHPRITGEEYDNFVNEFIQAVKSRW-PNVLLQ 244

HSP 2 Score: 33.4982 bits (75), Expect = 9.693e-15
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  245 FEDFAQKNAMPLLERYRDEVCCFNDDIQ 272

HSP 3 Score: 31.5722 bits (70), Expect = 9.693e-15
Identity = 14/29 (48.28%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L E + +FLGAG AG GIAE I  ++  +
Sbjct:  293 LCEQKVVFLGAGSAGCGIAEQIIAQMKSE 321

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SHEPA (NAD-dependent malic enzyme OS=Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 9.695e-15
Identity = 22/51 (43.14%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            IT+DVGT+N  LL+D  Y+G+R  R  G+EY E ++ FM AV + + + ++
Sbjct:  190 ITLDVGTDNPHLLEDPMYMGMRSPRIGGEEYTEFVEAFMQAVHRRWPDALI 240

HSP 2 Score: 35.4242 bits (80), Expect = 9.695e-15
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPLLERYKDQYCCFNDDIQ 269

HSP 3 Score: 32.7278 bits (73), Expect = 9.695e-15
Identity = 15/26 (57.69%), Postives = 18/26 (69.23%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEI 379
            L+E R  FLGAG AG GIAE I  ++
Sbjct:  290 LSEQRITFLGAGSAGCGIAEAIVAQM 315

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO11_PHOPR (NAD-dependent malic enzyme 1 OS=Photobacterium profundum GN=sfcA1 PE=3 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 1.254e-14
Identity = 23/51 (45.10%), Postives = 34/51 (66.67%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            + +DVGTNN Q L D  Y+G R  R +GQEY   +DEF+ AVK+ + + ++
Sbjct:  190 VVLDVGTNNPQRLSDPMYMGWRHTRISGQEYDNFVDEFIQAVKRRWPDALI 240

HSP 2 Score: 33.8834 bits (76), Expect = 1.254e-14
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y      FNDDIQ
Sbjct:  242 FEDFAQKNAMPLLERYKNKICCFNDDIQ 269

HSP 3 Score: 31.9574 bits (71), Expect = 1.254e-14
Identity = 15/26 (57.69%), Postives = 18/26 (69.23%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEI 379
            L+E R  FLGAG AG GIAE I  ++
Sbjct:  290 LSEQRISFLGAGSAGCGIAEAIIAQM 315

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SHELP (NAD-dependent malic enzyme OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=sfcA PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 1.254e-14
Identity = 23/51 (45.10%), Postives = 36/51 (70.59%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            IT+DVGT+N  LL+D  Y+G R +R  G+EYAE ++ FM AV + + + ++
Sbjct:  190 ITLDVGTDNPHLLEDPMYMGWRNQRIGGEEYAEFVEAFMEAVHRRWPDALI 240

HSP 2 Score: 35.4242 bits (80), Expect = 1.254e-14
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPLLERYKDRYCCFNDDIQ 269

HSP 3 Score: 31.187 bits (69), Expect = 1.254e-14
Identity = 14/26 (53.85%), Postives = 18/26 (69.23%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEI 379
            L++ R  FLGAG AG GIAE I  ++
Sbjct:  290 LSQQRIAFLGAGSAGCGIAEAIIAQM 315

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAO1_SHEHH (NAD-dependent malic enzyme OS=Shewanella halifaxensis (strain HAW-EB4) GN=sfcA PE=3 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 1.254e-14
Identity = 21/51 (41.18%), Postives = 35/51 (68.63%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVL 153
            +T+DVGT+N  LL+D  Y+G+R  R  G+EY E ++ FM AV + + + ++
Sbjct:  190 VTLDVGTDNPHLLEDPMYMGMRSPRIGGEEYKEFVEAFMQAVNRRWPDALI 240

HSP 2 Score: 35.4242 bits (80), Expect = 1.254e-14
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFA  NA  LL +Y   +  FNDDIQ
Sbjct:  242 FEDFAQKNAMPLLERYKDQYCCFNDDIQ 269

HSP 3 Score: 32.7278 bits (73), Expect = 1.254e-14
Identity = 15/26 (57.69%), Postives = 18/26 (69.23%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEI 379
            L+E R  FLGAG AG GIAE I  ++
Sbjct:  290 LSEQRITFLGAGSAGCGIAEAIVAQM 315

The following BLAST results are available for this feature:

BLAST of CF509642 vs. ExPASy Swiss-Prot

BLAST of CF509642 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 133


Match Name	E-value	Identity	Description
MAO1_SALDC	5.791e-15	49.02	NAD-dependent malic enzyme OS=Salmonella dublin (s...	[more]
MAO1_SALCH	5.791e-15	49.02	NAD-dependent malic enzyme OS=Salmonella choleraes...	[more]
MAO1_SALAR	5.791e-15	49.02	NAD-dependent malic enzyme OS=Salmonella arizonae ...	[more]
MAO1_SALA4	5.791e-15	49.02	NAD-dependent malic enzyme OS=Salmonella agona (st...	[more]
MAO12_PHOPR	7.496e-15	54.35	NAD-dependent malic enzyme 2 OS=Photobacterium pro...	[more]
MAO1_ERWT9	9.693e-15	49.06	NAD-dependent malic enzyme OS=Erwinia tasmaniensis...	[more]
MAO1_SHEPA	9.695e-15	43.14	NAD-dependent malic enzyme OS=Shewanella pealeana ...	[more]
MAO11_PHOPR	1.254e-14	45.10	NAD-dependent malic enzyme 1 OS=Photobacterium pro...	[more]
MAO1_SHELP	1.254e-14	45.10	NAD-dependent malic enzyme OS=Shewanella loihica (...	[more]
MAO1_SHEHH	1.254e-14	41.18	NAD-dependent malic enzyme OS=Shewanella halifaxen...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_125000-150 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-7_F07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF509642 ID=CF509642; Name=CF509642; organism=Citrus sinensis; type=EST; length=433bp
ATAACCGTTGATGTGGGTACAAACAATGAGCAATTGTTGAAGGATGAATT
CTACATTGGTCTACGACAGAGGAGGGCAACCGGACAGGAATATGCTGAAC
TTCTAGATGAATTCATGAGTGCTGTCAAGCAAAATTATGGAGAAAAAGTT
CTTATACAGGTTAGTTTGAGGATTTCGCAAACCACAATGCTTTTGAGTTG
CTTGCAAAATATGGCACAACTCATCTTGTCTTCAATGATGATATACAGCG
TCTGTTGTTCTTGCTGGGGTAGTTGCAGCACTGAAGTTGATTGGTGGCAC
ACTGGCCGAGCATAGATTTTTGTTTCTTGGAGCTGGGGAGGCTGGGACTG
GTATAGCTGAACTTATTGCGCTTGAGATATCAAAGCAGACTAAAGCTCCT
GTGGAAGAGACCCCGGAAGAAGATATGTCTTGT