CF832535

Overview
NameCF832535
Unique NameCF832535
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length651
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_THEEB (Ribosome-recycling factor OS=Thermosynechococcus elongatus (strain BP-1) GN=frr PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 2.119e-42
Identity = 83/143 (58.04%), Postives = 113/143 (79.02%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N A+LD++ V+YYG+   L+S+A ++TPD++++LIQPYD+S+L SIEKAI  SDLG+TPNNDG  +RL +P LT+ERRKEL K  AK AE GKVA+RNIRRDA+   +K  K  +L+ED VKDL   +QKLTD+Y+ KID++
Sbjct:   29 ANAALLDRVMVDYYGTETPLRSLANVSTPDATTILIQPYDRSTLASIEKAIQLSDLGLTPNNDGSSVRLNIPPLTTERRKELVKTAAKIAEGGKVAIRNIRRDAIDHIKKAGKAGELTEDEVKDLQEKVQKLTDKYIGKIDAL 171          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_SYNY3 (Ribosome-recycling factor OS=Synechocystis sp. (strain PCC 6803) GN=frr PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 2.119e-42
Identity = 83/143 (58.04%), Postives = 110/143 (76.92%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N ++LD+I VEYYG+   LKS+A I TPD+S+++IQP+D  S+ +IEKAI  SDLG+TPNNDG+VIRL +P LT+ERRKEL KV  K AEEGKVA+RNIRRDA+    K +K   +SED  +DL  ++QKLTD+  K+ID +
Sbjct:   29 ANASLLDRITVEYYGAETPLKSLATIGTPDASTIVIQPFDMGSIGTIEKAISLSDLGLTPNNDGKVIRLNIPPLTAERRKELVKVAGKLAEEGKVAIRNIRRDAVDEVRKQEKNSDISEDEARDLQEEIQKLTDQSTKRIDEL 171          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_CYAP7 (Ribosome-recycling factor OS=Cyanothece sp. (strain PCC 7424) GN=frr PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.614e-42
Identity = 80/143 (55.94%), Postives = 111/143 (77.62%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N A+LD++ V+YYG+   LKS+A I TPD++++ IQPYD+ S+  IEKAI  SD+G+TPNNDG++IRL +P LT+ERRKEL K+  K AEEGKVA+RNIRRDA+ +  K +K  +LSED  ++L  ++QK+TDEY  KID +
Sbjct:   29 ANSALLDRVMVDYYGTETPLKSLANITTPDATTIAIQPYDRGSMGQIEKAIQLSDIGLTPNNDGQMIRLNIPPLTTERRKELVKLAGKLAEEGKVAIRNIRRDAIDSVRKQEKNHELSEDESRNLQDEIQKVTDEYTAKIDEL 171          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_NOSP7 (Ribosome-recycling factor OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=frr PE=3 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 6.165e-42
Identity = 80/143 (55.94%), Postives = 113/143 (79.02%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N ++LDK+ V+YYGSP  LKS+A I+TPD+S++LIQPY++++L  IEKAI  SD+G+TP+NDG VIRL +P LTS+RRKE  K+  K AEEG+VA+RNIRRDA+ +  K +K  ++S+D  KD   +LQKLT+EY  +ID++
Sbjct:   29 ANASLLDKVLVDYYGSPTPLKSLANISTPDASTILIQPYERNTLNIIEKAISLSDVGLTPSNDGSVIRLNIPPLTSDRRKEFVKMATKYAEEGRVAIRNIRRDAIDSIRKQEKASEISKDESKDQQDNLQKLTNEYTSRIDAL 171          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_GEOSW (Ribosome-recycling factor OS=Geobacillus sp. (strain WCH70) GN=frr PE=3 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.794e-41
Identity = 79/146 (54.11%), Postives = 115/146 (78.77%), Query Frame = -2
Query:  213 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 650
            +NP++L+K+ V+YYG P  +  +A I+ P++  L+IQPYDKS +K IEKAI+SSDLG+TP+NDG VIR+T+P LT ERR+EL K+V K +E+ KVAVRNIRRDA    +KL+K  +++ED ++  + D+QKLTD+++ KID+I K+
Sbjct:   32 ANPSLLEKVTVDYYGMPTPIIQLASISVPEARLLVIQPYDKSVIKDIEKAILSSDLGLTPSNDGSVIRITIPPLTEERRRELVKLVKKYSEDAKVAVRNIRRDANDELKKLEKNGEITEDELRGYTDDVQKLTDDHIAKIDAITKE 177          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_BACSK (Ribosome-recycling factor OS=Bacillus clausii (strain KSM-K16) GN=frr PE=3 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.794e-41
Identity = 80/146 (54.79%), Postives = 112/146 (76.71%), Query Frame = -2
Query:  213 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 650
            +NPA+LD++ VEYYG+   L  +A +  P++  LLI PYDK+++ SIEKAI+ SDLG+TP+NDG+VIR+ +P LT ERRK+L ++V+K AEE KVAVRNIRRDA    +KLQK+ +++ED ++  + D+QKLTD Y+ KID   KQ
Sbjct:   32 ANPALLDRVTVEYYGAQTPLNQLASVTVPEARLLLITPYDKTAVASIEKAILKSDLGLTPSNDGQVIRIAIPPLTEERRKDLVRLVSKTAEESKVAVRNIRRDANDELKKLQKDGEMTEDELRSATDDIQKLTDTYVAKIDEKAKQ 177          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_TRIEI (Ribosome-recycling factor OS=Trichodesmium erythraeum (strain IMS101) GN=frr PE=3 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 3.996e-41
Identity = 80/143 (55.94%), Postives = 109/143 (76.22%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            +N ++LD++ VEYYGSP  LKS+A I  PD+S++ IQPYDK+SL  IEKAI  SD+G+TPNNDG +IRL +P LT+ERR+E+ K  AK AEEGKV++RNIRRDA+ +  K +K   +S+D  +DL   +QK TD+Y+ KID +
Sbjct:   29 ANASLLDRVMVEYYGSPTQLKSMANIGIPDASTITIQPYDKTSLGLIEKAINMSDVGLTPNNDGSIIRLNIPALTAERRQEMVKKTAKLAEEGKVSIRNIRRDAVDSIRKQEKNSDISKDESRDLQDKVQKKTDKYIAKIDEL 171          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_BACLD (Ribosome-recycling factor OS=Bacillus licheniformis (strain DSM 13 / ATCC 14580) GN=frr PE=3 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 5.219e-41
Identity = 80/145 (55.17%), Postives = 110/145 (75.86%), Query Frame = -2
Query:  216 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYK 650
            +NP++LDK+ VEYYG+   L  IA I  P++  L+I PYDK++L  IEKAI  +DLG+TP+NDG +IR+T+P LT ERRKEL+K+V K +E+ KVAVRNIRRDA    +KL+K  +++ED ++  + D+QKLTDEY+ KID I K
Sbjct:   32 ANPSLLDKVAVEYYGAQTPLNQIASITVPEARMLVITPYDKTALGDIEKAIQKADLGVTPSNDGNIIRITIPPLTEERRKELAKLVKKYSEDAKVAVRNIRRDANDDLKKLEKNGEMTEDELRSSTEDVQKLTDEYVSKIDEITK 176          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_BACHD (Ribosome-recycling factor OS=Bacillus halodurans GN=frr PE=3 SV=3)

HSP 1 Score: 167.548 bits (423), Expect = 5.219e-41
Identity = 80/141 (56.74%), Postives = 113/141 (80.14%), Query Frame = -2
Query:  228 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKID 650
            +NPAMLD+I VEYYG+   L  +A I+ P++  L+IQP+DKSS+  IE+AI  SDLG+TP+NDG VIR+T+P LT ERR++L+K+V K AEE KVAVRNIRRDA    +K QK+ +L+ED+++ ++ D+QKLTD+Y+++ID
Sbjct:   32 ANPAMLDRITVEYYGAETPLNQLATISVPEARLLVIQPFDKSSISDIERAIQKSDLGLTPSNDGTVIRITIPPLTEERRRDLTKLVKKSAEEAKVAVRNIRRDANDDLKKRQKDGELTEDDLRRVTEDVQKLTDKYIEQID 172          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_SYNP2 (Ribosome-recycling factor OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=frr PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 8.903e-41
Identity = 80/145 (55.17%), Postives = 107/145 (73.79%), Query Frame = -2
Query:  216 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYK 650
            ++ ++LD+I VEYYG+   LKS+A I+TPDSS+++IQP+D+ SL  +E+AI  SDLG+TPNNDG  IRL +P LT ERR+EL K   K AEEGKVA+RNIRRDA+    K +K   +SED  + L  D+QK+TDE+  KID + K
Sbjct:   29 ASASLLDRITVEYYGAETPLKSLASISTPDSSTIMIQPFDRGSLSDVERAISMSDLGLTPNNDGTNIRLNIPPLTKERRQELVKTAGKLAEEGKVALRNIRRDAIDDVRKQEKNSDISEDESRSLQDDIQKVTDEFTTKIDDLLK 173          
The following BLAST results are available for this feature:
BLAST of CF832535 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RRF_THEEB2.119e-4258.04Ribosome-recycling factor OS=Thermosynechococcus e... [more]
RRF_SYNY32.119e-4258.04Ribosome-recycling factor OS=Synechocystis sp. (st... [more]
RRF_CYAP73.614e-4255.94Ribosome-recycling factor OS=Cyanothece sp. (strai... [more]
RRF_NOSP76.165e-4255.94Ribosome-recycling factor OS=Nostoc punctiforme (s... [more]
RRF_GEOSW1.794e-4154.11Ribosome-recycling factor OS=Geobacillus sp. (stra... [more]
RRF_BACSK1.794e-4154.79Ribosome-recycling factor OS=Bacillus clausii (str... [more]
RRF_TRIEI3.996e-4155.94Ribosome-recycling factor OS=Trichodesmium erythra... [more]
RRF_BACLD5.219e-4155.17Ribosome-recycling factor OS=Bacillus licheniformi... [more]
RRF_BACHD5.219e-4156.74Ribosome-recycling factor OS=Bacillus halodurans G... [more]
RRF_SYNP28.903e-4155.17Ribosome-recycling factor OS=Synechococcus sp. (st... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02A06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02A06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832535 ID=CF832535; Name=CF832535; organism=Citrus sinensis; type=EST; length=651bp
TTTTTTTTTTTTTTTTTACAAAGAGTCTAGAATAACTAATTTCCCCCTTT
CATTTCATAACAAAATATCCACCCGTGTCTCCCATTATTATCAAAAAATG
CCATTCTATGGGCAAACCAAAAAATAAAGAGTGCATTCTGAGATTCACTA
GAGAGCTCACCCTTACATTTGTGTGTAAACAATCATTAAACCTTCAACAA
CTCCTTCTCTTTCTGTTTGTAGATGCTATCAATCTTCTTCATATACTCAT
CTGTTAGTTTCTGTAAATCACTTGACAAGTCCTTCACATTGTCTTCAGAG
AGCTTCTTCTCCTTCTGAAGTTTTTCATAAGCTTTTAACGCATCTCTTCT
TATATTCCTAACTGCCACCTTCCCTTCTTCAGCCTGTTTGGCCACAACCT
TTGACAGTTCCTTCCTCCTTTCGGATGTCAATTGAGGAAGGGTTAACCGT
ATGACTTCACCATCATTGTTTGGGGTCATGCCAAGATCAGAGCTCACTAT
GGCCTTCTCTATAGACTTTAAGCTGGATTTGTCATATGGTTGAATCAATA
GAGAACTTGAATCAGGGGTATTGATTTGAGCTATGCTCTTCAAACTTACT
GGGCTTCCATAGTATTCCACCTCAATCTTATCTAGCATAGCTGGATTCGA
T
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